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HdeA在细菌外膜囊泡环境下的原位NMR研究

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波谱学杂志 2024年第1期41卷 1-8页

作者：王欢陶志清姜国胜张许王冠禾立春刘买利潍坊医学院山东潍坊261053 中国科学院大学北京100049 波谱与原子分子物理国家重点实验室武汉磁共振中心中国科学院精密测量科学与技术创新研究院湖北武汉430071

HdeA是一种定位于细菌周质的分子伴侣,在维持蛋白质稳态中起着重要的作用.以往对HdeA的研究主要是在体外条件下进行,限制了人们对HdeA在天然环境下发挥作用机制的理解.细菌外膜囊泡是细菌自发分泌到胞外环境的外膜囊泡,其内容物与周质... 详细信息

HdeA是一种定位于细菌周质的分子伴侣,在维持蛋白质稳态中起着重要的作用.以往对HdeA的研究主要是在体外条件下进行,限制了人们对HdeA在天然环境下发挥作用机制的理解.细菌外膜囊泡是细菌自发分泌到胞外环境的外膜囊泡,其内容物与周质环境相似.本研究将HdeA富集到细菌外膜囊泡(OMVs)中,通过核磁共振波谱研究HdeA在OMVs中的构象变化.结果表明,HdeA在其原位环境中表现出酸依赖性的构象变化.在低pH条件下HdeA主要通过S15、W16、T17、S27、T32、E36、G54、T57、C66、Q71、F74及D83等残基启动其分子伴侣功能.此外本研究也为原位研究其它周质分子伴侣的功能提供了新方法.

关键词：原位核磁共振波谱 HdeA 外膜囊泡分子伴侣

Structural and functional imaging of brains

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Science China Chemistry 2023年第2期66卷 324-366页

作者： Zhichao liu Ying Zhu Liming Zhang Weiping Jiang Yawei liu Qiaowei Tang Xiaoqing Cai Jiang Li Lihua Wang Changlu Tao Xianzhen Yin Xiaowei Li Shangguo Hou Dawei Jiang Kai liu Xin Zhou Hongjie Zhang maili liu Chunhai Fan Yang Tian Shanghai Key Laboratory of Green Chemistry and Chemical Processes School of Chemistry and Molecular EngineeringEast China Normal UniversityShanghai200241China Interdisciplinary Research Center Shanghai Synchrotron Radiation FacilityZhangjiang LaboratoryShanghai Advanced Research InstituteChinese Academy of SciencesUniversity of Chinese Academy of SciencesShanghai201210China State Key Laboratory of Magnetic Resonance and Atomic and Molecular Physics Chinese Academy of SciencesWuhan National Laboratory for OptoelectronicsWuhan430071China State Key Laboratory of Rare Earth Resource Utilization Changchun Institute of Applied ChemistryChinese Academy of SciencesChangchun130022China Department of Chemistry Tsinghua UniversityBeijing100084China School of Chemistry and Chemical Engineering Frontiers Science Center for Transformative MoleculesInstitute of Translational MedicineShanghai Jiao Tong UniversityShanghai200240China Interdisciplinary Center for Brain Information Brain Cognition and Brain Disease InstituteShenzhen-Hong Kong Institute of Brain Science-Shenzhen Fundamental Research InstitutionsFaculty of Life and Health SciencesShenzhen Institute of Advanced TechnologyChinese Academy of SciencesShenzhen518055China Lingang Laboratory Shanghai201602China School of Biomedical Engineering Shanghai Jiao Tong UniversityShanghai200240China Institute of Systems and Physical Biology Shenzhen Bay LaboratoryShenzhen518055China Department of Nuclear Medicine Union HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhan430022China

Analyzing the complex structures and functions of brain is the key issue to understanding the physiological and pathological *** neuronal morphology and local distribution of neurons/blood vessels in the brain have been known,the subcellular structures of cells remain challenging,especially in the live *** addition,the complicated brain functions involve numerous functional molecules,but the concentrations,distributions and interactions of these molecules in the brain are still poorly *** this review,frontier techniques available for multiscale structure imaging from organelles to the whole brain are first overviewed,including magnetic resonance imaging(MRI),computed tomography(CT),positron emission tomography(PET),serial-section electron microscopy(ss EM),light microscopy(LM)and synchrotron-based X-ray microscopy(XRM).Specially,XRM for three-dimensional(3D)imaging of large-scale brain tissue with high resolution and fast imaging speed is ***,the development of elegant methods for acquisition of brain functions from electrical/chemical signals in the brain is *** particular,the new electrophysiology technologies for neural recordings at the single-neuron level and in the brain are also *** also focus on the construction of electrochemical probes based on dual-recognition strategy and surface/interface chemistry for determination of chemical species in the brain with high selectivity and long-term stability,as well as electrochemophysiological microarray for simultaneously recording of electrochemical and electrophysiological signals in the ***,the recent development of brain MRI probes with high contrast-to-noise ratio(CNR)and sensitivity based on hyperpolarized techniques and multi-nuclear chemistry is ***,multiple optical probes and instruments,especially the optophysiological Raman probes and fiber Raman photometry,for imaging and biosensing in live brain are emphas

关键词： brain structure brain function brain chemistry chemical signal biosensing and bioimaging

Preface to the Special Issue “Celebrating the 80th Birthday of Professor Chaohui Ye”

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Magnetic Resonance Letters 2022年第3期2卷 I0004-I0005页

作者： maili liu Feng Deng Xin Zhou 不详

It’s hard to imagine the unlimitedness of human life until you see it with your own *** Chaohui Ye demonstrates to us how unlimited and meaningful a life can be,by maximizing his talents and efforts in every *** is such a gentleman,a great scientist,an encouraging supervisor,an insightful research group leader and a profound thinker,who inspired and influenced so many,including the authors and guest editors of this special issue.

关键词： meaningful hao insight

Protein dynamics elucidated by NMR technique

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Protein & Cell 2013年第10期4卷 726-730页

作者： Conggang Li Chun Tang maili liu Key Laboratory of Magnetic Resonance in Biological Systems Wuhan Center for Magnetic ResonanceState Key Laboratory of Magnetic Resonance and Atomic and Molecular PhysicsWuhan Institute of Physics and MathematicsChinese Academy of SciencesWuhan 430071China

In 1957,Christian Anfinsen postulated his famous dogma based on the study of ribonuclease(Sela et al.,1957),stating that the amino acid sequence of a protein determines its tertiary structure,a unique and kinetically accessible energy minimum,in a given *** a result,determination of a global energy minimum,or the most populated structure,has been the main focus of structural biology in the ensuing half a *** more and more structures have been determined,people have gain some insights of why protein functions,but the detailed mechanisms and the function process,or how protein functions,remains elusive.

关键词： elucidated structure dynamics

New Researches of State Key Laboratories in Analytical Chemistry

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Science China Chemistry 2016年第7期59卷 781-782页

作者： Huangxian Ju Weihong Tan Lehui Lu maili liu state key laboratory of analytical chemistry for life science the university of michigan in the us the changchun institute of applied chemistry chinese academy of sciences (ciaccas) the changchun institute of applied chemistry chinese academy of sciences (ciaccas)the state key laboratory of electroanalytical chemistry the state key laboratory of magnetic resonance and atomic and molecular physics

In the past two decades,as the eyes of fundamental scientific research and social activities relative to human security and health,analytical chemistry has made great progress in both analytical methodologies and detection instruments by integrating multidisciplinary research *** State Key Laboratories were successively established in this rapidly developing *** order to publish some of the excellent results together,we have organized this special topic with 10 Articles and Reviews from these State Key Laboratories.

关键词：国家重点实验室分析化学社会活动基础科学检测仪器安全性科研

Mechanisms of Chaperones as Active Assistant/Protector for Proteins:Insights from NMR Studies

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Chinese Journal of Chemistry 2020年第4期38卷 406-413页

作者： Yunfei Hu Conggang Li Lichun He Changwen Jin maili liu WuhanHubei 430071China University of Chinese Academy of Sciences Beijing 100049China Beijing Nuclear Magnetic Resonance Center College of Chemistry and Molecular EngineeringCollege of Life SciencesPeking UniversityBeijing 100871China

Molecular chaperones are diverse families of proteins that play key roles in protein *** assist the folding of client proteins or prevent them from rreversible aggregation under stress *** chaper-one families contribute to different aspects of protein homeostasis by inter-acting with a wide range of client *** the vital roles of chaper-ones in cell survival,the molecular mechanisms underlying chaperone func-tions remain elusive,due to the non-specificity of chaperone-client interac-tions and the intrinsic flexibility of the clients.

关键词： client Active homeostasis

Simultaneous acquisition of multi-nuclei enhanced NMR/MRI by solution-state dynamic nuclear polarization

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Science China Chemistry 2016年第7期59卷 830-835页

作者： Yugui He Zhen Zhang Jiwen Feng Chongyang Huang Fang Chen Chaoyang liu maili liu State Key Laboratory of Magnet Resonance and Atomic and Molecular Physics Wuhan Institute of Physics and MathematicsChinese Academy of SciencesWuhan 430071China School of Optical and Electronic Information Huazhong University of Science and TechnologyWuhan 430074China University of Chinese Academy of Sciences Beijing 100048China

Dynamic nuclear polarization （DNP） has become a very important hyperpolarization method because it can dramatically increase the sensitivity of nuclear magnetic resonance （NMR） of various molecules. Liquid-state DNP based on Overhauser effect is capable of directly enhancing polarization of all kinds of nuclei in the system. The combination of simultaneous Overhauser multi-nuclei enhancements with the multi-nuclei parallel acquisitions provides a variety of important applications in both MR spectroscopy （MRS） and image （MRI）. Here we present two simple illustrative examples for simultaneously enhanced multi-nuclear spectra and images to demonstrate the principle and superiority. We have observed very large simultaneous DNP enhancements for different nuclei, such as XH and 23Na, 1H and 31p, 19F and 31p, especially for the first time to report sodium ion enhancement in liquid. We have also obtained the simultaneous images of 19H and 31p, 19F and 31p at low field by solution-state DNP for the first time.

关键词： dynamic nuclear polarization Overhauser effect simultaneous acquisition phosphorous and sodium ion DNP spectra phosphorous DNP image

Impact of Magnesium(Ⅱ) on Beryllium Fluorides in Solutions Studied by ^(19)F NMR Spectroscopy

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Chinese Journal of Chemistry 2014年第9期32卷 878-882页

作者： Yixiang liu Xian Mao maili liu Ling Jiang Key Laboratory of Magnetic Resonance in Biological Systems Wuhan Center for Magnetic ResonanceState Key Laboratory of Magnetic Resonance and Atomic and Molecular PhysicsWuhan Institute of Physics and MathematicsChinese Academy of SciencesWuhanHubei 430071China Department of Pharmacology Case Western Reserve University School of MedicineClevelandOH 44106USA Graduate University of Chinese Academy of Sciences Beijing 100049China

Beryllium fluorides are widely used in protein phosphorylation studies to get stable transition state analogs or near attack conformers,which has attracted much ***_(3)^(−) is one of the optimal phosphoryl(PO_(3)^(−))analogs for its identical geometry and charge,and Mg^(2+) naturally participates in the phosphoryl binding in biological *** solutions,BeF_(3)^(−) coexists with other beryllium fluorides(BeF_(4)^(2−),BeF_(2) and BeF^(+))and magnesium fluorides,and there are equilibriums between these *** this article,^(19)F NMR spectroscopy was applied to the investigation of the impact of magnesium(Ⅱ)on beryllium *** has been found that when Mg^(2+) was introduced into the solutions,the chemical shifts,the intensities and the line widths of ^(19)F signals of various beryllium fluoride complexes were *** ionic strength correction,these effects were remarkable only for 2 BeF_(4)^(2−) and BeF_(3)^(−),especially BeF_(4)^(2−),when the concentration of the fluoride ion is relatively *** of the effects is proposed which involves ion pair formation between Mg^(2+) and beryllium fluorides.

关键词： beryllium fluorides magnesium(Ⅱ) ^(19)F NMR ion pair protein phosphorylation

NMR assignments and characterization of the DNA-binding domain of Arabidopsis transcription factor WRKY11--Dedicated to Professor Xiuwen Han on the occasion of her 80th birthday

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Magnetic Resonance Letters 2021年第2期1卷 112-120页

作者： Jiannan Wang Yaling Lin Qinjun Zhu Jingfeng Zhang maili liu Yunfei Hu State Key Laboratory of Magnetic Resonance and Atomic and Molecular Physics Wuhan National Laboratory for OptoelectronicsNational Center for Magnetic Resonance in WuhanKey Laboratory of Magnetic Resonance in Biological SystemsInnovation Academy for Precision Measurement Science and TechnologyChinese Academy of SciencesWuhan430071China University of Chinese Academy of Sciences Beijing100049China

The WRKY proteins are a family of plant-specific transcription factors(TFs)that are widely involved in plant development and anti-stress *** WRKY11(AtWRKY11)functions in regulating plant defense against abiotic stress and belongs to the Ild subgroup of WRKY *** herein report the expres sion,purification and preliminary structural characterization of AtWRKY11 DNA-binding domain(DBD)using solution NMR Almost complete backbone chemical shift assignments of AtWRKY11-DBD have been *** shift-based secondary structure analysis suggests that AtWRKY11-DBD may exhibit local conformational diferences from the X-ray structure of the C-terminal WRKY domain of AtWRKY1,particularly in the β1 and β5 *** current study provides the basis for further structural and interactional studies.

关键词： Transcription factor WRKY DNA-Binding domain W-box Nuclear magnetic resonance(NMR) Protein-DNA interaction