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Sequential use of transarterial chemoembolization and percutaneous cryosurgery for hepatocellular carcinoma

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World Journal of Gastroenterology 2009年第29期15卷 3664-3669页

作者： ke-Cheng Xu li-Zhi Niu Qiang Zhou Yi-Ze Hu De-Hong Guo Zheng-Ping liu Bing Lan Feng mu Ying-Fei li Jian-Sheng Zuo Cryosurgery Center for Cancer Fuda CancerHospital Guangzhou 510300 Guangdong Province China Department of Hepato-biliary Surgery SecondAffiliated Hospital Guangzhou Medical College Guangzhou510300 Guangdong Province China OncologyDepartment Fuda Hospital Guangzhou 510300 GuangdongProvince China Department of Gastroenterology NanfangHospital Nanfang Medical University Guangzhou 510300Guangdong Province China

AIM: To evaluate the efficacy of sequential use of transarterial chemoembolization (TACE) and percutaneous cryosurgery for unresectable hepatocellular carcinoma (HCC). METHODS: Four hundred and twenty patients were enrolled in this study. The patients, who were considered to have unresectable tumors due to their location or size or comorbidity, were divided into sequential TACE-cryosurgery (sequential) group (n = 290) and cryosurgery alone (cryoalone) group (n = 130). Patients in the sequential group tended to have larger tumors and a greater number of tumors than those in the cryo-alone group. tumors larger than 10 cm in diameter were only seen in the sequential group. TACE was performed with the routine technique and percutaneous cryosurgery was conducted under the guidance of ultrasound 2-4 wk after TACE. RESULTS: During a mean follow-up period of 42 ± 17 mo (range, 24-70 mo), the local recurrence rateat the ablated area was 17% for all patients, 11% and 23% for patients in sequential group and cryoalone groups, respectively (P = 0.001). The overall 1-, 2-, 3-, 4and 5-year survival rate was 72%, 57%, 47%, 39% and 31%, respectively. The 1and 2-year survival rates (71% and 61%) in sequential group were similar to those (73% and 54%) in cryo-alone group (P = 0.69 and 0.147), while the 4and 5-year survival rates were 49% and 39% in sequential group, higher than those (29% and 23%) in cryo-alone group (P = 0.001). Eighteen patients with large HCC (> 5 cm in diameter) survived for more than 5 years after sequential TACE while no patient with large HCC (> 5 cm in diameter) survived more than 5 years after cryosurgery. The overall complication rate was 24%, and the complication rates were 21% and 26% for the sequential and cryo-alone groups, respectively (P = 0.06). The incidence of hepatic bleeding was higher in cryo-alone group than in sequential group (P = 0.02). liver crack only occurred in two patients of the cryoalone group. CONCLUSION: Pre-cryosurgical TACE can in

关键词： Hepatocellular carcinoma Cryosurgery Transarterial chemoembolization Cryoablation Treatment

23价肺炎球菌多糖疫苗在≥2岁健康人群中安全性和免疫原性Ⅳ期临床试验评价

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中国疫苗和免疫 2024年第2期30卷 184-190页

作者：钱小爱刘凯杨北方王硕柯华柯昌显李锦霞张治国穆飞军张馨月周婷婷童叶青官旭华魏晟郑景山张险峰湖北省疾病预防控制中心湖北武汉430079 北京民海生物科技有限公司北京102600 十堰市郧阳区疾病预防控制中心湖北十堰442500 荆州市监利市疾病预防控制中心湖北荆州433300 华中科技大学同济医学院公共卫生学院湖北武汉430030

目的评价≥2岁健康人群接种23价肺炎球菌多糖疫苗(Pneumococcal polysaccharide vaccine,PPV23)的安全性和免疫原性。方法采用开放性Ⅳ期临床试验,招募2-5岁、6-15岁、16-59岁和≥60岁健康受试者,观察1剂次PPV23接种后30 d内不良事件,... 详细信息

目的评价≥2岁健康人群接种23价肺炎球菌多糖疫苗(Pneumococcal polysaccharide vaccine,PPV23)的安全性和免疫原性。方法采用开放性Ⅳ期临床试验,招募2-5岁、6-15岁、16-59岁和≥60岁健康受试者,观察1剂次PPV23接种后30 d内不良事件,分析发生率;检测接种前和接种后30 d血清标本23种肺炎链球菌(Streptococcus pneumoniae,Spn)血清型IgG抗体,评价接种后抗体阳转率、几何平均浓度(Geometric mean concentration,GMC)和几何平均增长倍数(Geometric mean increase,GMI)。结果在2997例受试者中,PPV23接种后与疫苗有关的不良事件发生率为10.84%(325例),其中1级、2级、3级、4级不良事件分别为9.94%、0.77%、0.13%、0.00%;接种部位疼痛、发热分别为4.50%、1.40%。在273例受试者中,PPV23接种后23种Spn血清型IgG抗体阳转率在92.83%-99.66%之间,GMC在2.00-63.34μg/mL之间,GMI在6.82-171.24之间。结论≥2岁健康人群接种1剂次PPV23具有良好的安全性和免疫原性。

关键词： 23价肺炎球菌多糖疫苗安全性免疫原性 Ⅳ期临床试验

Preparation of AlN film grown on sputter-deposited and annealed AlN buffer layer via HVPE

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Chinese Physics B 2021年第3期30卷 430-435页

作者： Di-Di li Jing-Jing Chen Xu-Jun Su Jun Huang mu-Tong Niu Jin-Tong Xu ke Xu Suzhou Institute of Nano-tech and Nano-bionics Chinese Academy of SciencesSuzhou 215123China Suzhou Nanowin Science and Technology Co. Ltd.Suzhou 215123China School of Physical Science and Technology ShanghaiTech UniversityShanghai 201210China key Laboratory of Infrared Imaging Materials and Detectors Shanghai Institute of Technical PhysicsChinese Academy of SciencesShanghai 200083China

AlN films grown on sputter-deposited and annealed AlN buffer layer by high temperature hydride vapor phase epitaxy(HVPE)have been fabricated and structurally *** crystalline quality and surface morphology of as-grown AlN films with various V/III ratios were studied and *** XRD results showed that the crystalline quality of the AlN film could be optimized when the growth V/III ratio was *** the same time,the full width at half-maximum(FWHM)values of(0002)-and(10¯12)-plane were 64 arcsec and 648 arcsec,*** revealed by AFM,the AlN films grown with higher V/III ratios of 150 and 300 exhibited apparent hillock-like surface structure due to the low density of screw threading dislocation(TD).The defects microstructure and strain field around the HVPE-AlN/sputtered-AlN/sapphire interfaces have been investigated by transmission electron microscopy(TEM)technique combined with geometric phase analysis(GPA).It was found that the screw TDs within AlN films intend to turn into loops or half-loops after originating from the AlN/sapphire interface,while the edge ones would bend first and then reacted with others within a region of 400 nm above the ***,part of the edge TDs propagated to the surface *** GPA analysis indicated that the voids extending from sapphire to HVPE-AlN layer were beneficial to relax the interfacial strain of the best quality AlN film grown with a V/III ratio of 150.

关键词： hydride vapor phase epitaxy(HVPE) AlN threading dislocation(TD) sputter-deposition

CRISPR-Cas9技术的原理及其在猪研究中的应用

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生命科学 2018年第6期30卷 690-700页

作者：李晓开龙科任麦苗苗王讯四川农业大学动物科技学院动物遗传育种研究所成都611130 畜禽遗传资源发掘与创新利用四川省重点实验室成都611130

成簇的规律间隔的短回文重复序列(clustered regularly interspaced short palindromic repeats,CRISPR)及其相关蛋白(CRISPR-associated proteins,Cas)是细菌和古生菌中抵抗外源病毒或质粒入侵的获得性免疫系统,目前已经被广泛应用于动物基因组编辑。现回顾CRISPR-Cas9系统的发展历程并比较其与锌指核酸酶(ZFNs)、类转录激活因子效应物核酸酶(TALENs)技术的优势,详细介绍了CRISPR-Cas9系统的组成成分和各组分的功能以及其编辑基因组的原理,着重梳理了CRISPR-Cas9系统在猪生产性能、抗病育种、人类模式动物构建和异种器官移植方面的最新研究进展,以期为CRISPR-Cas9系统的进一步应用提供参考。

关键词： CRISPR-Cas9 基因组编辑原理猪

A novel frameshift mutation in CX46 associated with hereditary dominant cataracts in a Chinese family

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International Journal of Ophthalmology(English edition) 2017年第5期10卷 684-690页

作者： Xiu-Kun Cui ke-ke Zhu Zheng Zhou Si-Min Wan Yi Dong Xuan-Ce Wang Jing li Jing Zhang Hong-Mei mu Lei Qin Yan-Zhong Hu Henan Intemational Union Laboratory of Antibody Medicine Department of Cell biology and Genetics Henan University School of Medicine Kaifeng 475004 Henan Province China Kaifeng key Lab of Cataract and Myopia Institute of Eye Diseases Kaifeng Centre Hospital Kaifeng 475000 Henan Province China Department of Cardiovascular Medicine Kaifeng Centre Hospital Kaifeng 475000 Henan Province China

AIM：To investigate the genetic mutations that are associated the hereditary autosomal dominant cataract in a Chinese ***：A Chinese family consisting of 20 cataract patients（including 9 male and 11 female） and 2 unaffected individuals from 5 generations were diagnosed to be a typical autosomal dominant cataract pedigree. Genomic DNA samples were extracted from the peripheral blood cells of the participants in this pedigree. Exon sequence was used for genetic mutation screening. In silico analysis was used to study the structure characteristics of connexin 46（CX46） mutant. Immunoblotting was conduceted for testing the expression of ***：To determine the involved genetic mutations, 11 well-known cataract-associated genes（cryaa, cryab, crybb1, crybb2, crygc, crygd, Gja3, Gja8, Hsf4, Mip and Pitx3） were chosen for genetic mutation test by using exon sequencing. A novel cytosine insertion at position 1195 of CX46 c DNA（c.1194_1195ins C） was found in the samples of 5 tested cataract patients but not in the unaffected 2 individuals nor in normal controls, which resulted in 30 amino acids more extension in CX46Cterminus（cx46fs400） compared with the wild-type CX46. In silico protein structure analysis indicated that the mutant showed distinctive hydrophobicity and protein secondary structure compared with the wild-type CX46. The immunoblot results revealed that CX46 protein, which expressed in the aging cataract lens tissues, was absence in the proband lens. In contrast, CX50, alpha A-crystallin and alphaB-crystallin expressed equally in both proband and aging cataract tissues. Those results revealed that the cx46fs400 mutation could impair CX46 protein expression. CONCLUSION：The insertion of cytosine at position 1195 of CX46 cD NA is a novel mutation site that is associated with the autosomal dominant cataracts in this Chinese family. The C-terminal frameshift mutation is involved in regulating CX46 protein expression.

关键词： connexin 46 cataract congenital ocular lens autosome dominant heredity genetic mutation

黑果小檗内生真菌假丝酵母菌次生代谢产物研究

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中成药 2024年第9期46卷 3000-3005页

作者：郭铭焯麻淑芳王世苗冯亚萍欧阳艳庞克坚焦子伟木古丽木哈西杨新洲伊犁师范大学新疆伊宁835000 中南民族大学药学院湖北武汉430074

目的研究黑果小檗内生真菌假丝酵母菌Candida sp.的次生代谢产物。方法假丝酵母菌发酵提取物次级代谢产物的乙酸乙酯部位、石油醚部位采用硅胶、Sephadex LH-20、制备液相色谱进行分离纯化,根据理化性质及波谱数据鉴定所得化合物的结构... 详细信息

目的研究黑果小檗内生真菌假丝酵母菌Candida sp.的次生代谢产物。方法假丝酵母菌发酵提取物次级代谢产物的乙酸乙酯部位、石油醚部位采用硅胶、Sephadex LH-20、制备液相色谱进行分离纯化,根据理化性质及波谱数据鉴定所得化合物的结构。结果从中分离得到18个化合物,分别鉴定为1-苯基-1,2-乙二醇(1)、4-羟基苯乙醇(2)、4-羟基苯甲酸(3)、对羟基苯乙酸(4)、间羟基苯乙酸(5)、3-甲基亚砜基丙酸(6)、苯乙酸(7)、(S)-N-nitroso-1-amino-p-hydroxy phenylethanol(8)、2-苯乙酰胺(9)、对羟基苯甲醛(10)、2-(4-羟苯基)-乙酸乙酯(11)、邻苯二甲酸二丁酯(12)、5,5′-dimethoxybiphenyl-2,2′-diol(13)、3-吲哚甲醛(14)、N-乙酰-L-苯丙氨酸(15)、9-hydroxy-10 E,12Z-octadecadienoic acid(16)、9-hydroxy-10 E,12E-octadecadienoic acid(17)、(6E)-5-methylene-6-tetradecenoic acid(18)。结论化合物1、3~8、10~18为首次从假丝酵母菌真菌中分离得到。

关键词：黑果小檗假丝酵母菌内生真菌次生代谢产物分离鉴定

2-羟基-3-甲氧基苯基荧光酮与钛显色反应的研究及应用

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冶金分析 2005年第2期25卷 10-12页

作者：常世科汤家华李在均潘教麦巢湖学院化学系华东师范大学化学系安徽巢湖238000 江南大学化学与材料工程学院上海200062 江苏无锡214036

研究了2 羟基3 甲氧基苯基荧光酮(HMPF)与钛的显色反应,建立了一种直接测定钢铁中微量钛的光度分析方法。在硫酸介质中,表面活性剂OP作用下,2 羟基3 甲氧基苯基荧光酮与钛形成一种红色络合物,络合物的组成为钛∶HMPF=1∶3(物质的量比),... 详细信息

研究了2 羟基3 甲氧基苯基荧光酮(HMPF)与钛的显色反应,建立了一种直接测定钢铁中微量钛的光度分析方法。在硫酸介质中,表面活性剂OP作用下,2 羟基3 甲氧基苯基荧光酮与钛形成一种红色络合物,络合物的组成为钛∶HMPF=1∶3(物质的量比),其最大吸收峰位于536nm,表观摩尔吸光系数为1 9×105,络合物在7h内稳定。在25mL溶液中,钛质量在0～3μg范围内符合比尔定律。显色反应有良好的选择性,方法简单、灵敏,可不经分离直接测定钢铁中微量钛,结果满意。

关键词： 2羟基3甲氧基苯基荧光酮分光光度法钛钢铁

High levels of circulating GM-CSF^(+) CD4^(+) T cells are predictive of poor outcomes in sepsis patients: a prospective cohort study

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Cellular & Molecular Immunology 2019年第6期16卷 602-610页

作者： Huihuang Huang Siyu Wang tianjun Jiang Rong Fan Zheng Zhang Jinsong mu ke li Yonggang Wang Lei Jin Fang lin Jie Xia lijian Sun Biao Xu Chengcheng Ji Jing Chen Juan Chang Bo tu Bing Song Chao Zhang Fu-Sheng Wang Ruonan Xu Treatment and Research Center for Infectious Disease Beijing 302 HospitalBeijingChina Clinical Research and Management Center Beijing 302 HospitalBeijingChina Intensive Care Center Beijing 302 HospitalBeijingChina Treatment and Research Center for liver Failure Beijing 302 HospitalBeijingChina Department of Infectious Diseases The Affiliated Southwest Hospital of the Third Military Medical UniversityChongqingChina Medical Department Army General Hospital of PLABeijingChina

Granulocyte colony-stimulating factor(GM-CSF),produced by CD4^(+)T cells,has recently been implicated in the pathogenesis of inflammatory diseases,such as multiple sclerosis and juvenile ***,the role of GM-CSF-producing CD4^(+)T cells in sepsis remains *** study reports peripheral changes in GM-CSF-producing CD4^(+)T cells in septic patients and the possible underlying mechanism by which GM-CSF influences the outcome of ***-three septic patients,20 SIRS patients,and 20 healthy controls were enrolled in this study and followed for 28 days to assess *** measured the peripheral frequency of GM-CSF^(+)CD4^(+)T cells and recorded their associated relationship with disease *** data demonstrated that peripheral GM-CSF-producing CD4^(+)T cells were significantly higher in septic patients than in both SIRS patients and healthy *** cells exhibit a memory phenotype and impaired IFN-γ-secreting capacity in sepsis *** a receiver operating curve analysis with 8.01%as a cut-off point,the percentage of GM-CSF^(+)CD4^(+)T cells could predict the outcome of septic *** with the increase in GM-CSF-producing CD4^(+)T cells,inflammatory cytokines IL-1βand IL-6 were also *** an in vitro neutrophil model,we found that GM-CSF inhibited C3aR expression,while inducing IL-8 ***,this effect was transferrable in plasma from sepsis patients and was attenuated by inhibition of GM-CSF using an anti-GM-CSF *** results indicate that GM-CSF-producing CD4^(+)T cells may serve as a marker of sepsis ***,targeting GM-CSF overproduction may benefit sepsis patients.

关键词： GM-CSF CD4^(+)T cells sepsis SIRS

Endoscopic transgastric fenestration versus percutaneous drainage for management of(peri)pancreatic fluid collections adjacent to gastric wall(with video)

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World Journal of Gastroenterology 2023年第40期29卷 5557-5565页

作者： Hong-Mei Zhang Hui-ting ke Md Robin Ahmed Ya-Juan li Ghulam Nabi mu-Han li Ji-Yu Zhang Dan liu li-Xia Zhao Bing-Rong liu Department of Gastroenterology and Hepatology The First Affiliated Hospital of Zhengzhou UniversityZhengzhou 450000Henan ProvinceChina Department of Ultrasound Ezhou Central HospitalEzhou 436099Hubei ProvinceChina Institute of Nature Conservation Polish Academy of SciencesKrakow 31120Poland State key Laboratory of Esophageal Cancer Prevention and Treatment Zhengzhou UniversityZhengzhou 450052Henan ProvinceChina

BACKGROUND Percutaneous drainage(PCD)and endoscopic approaches have largely replaced surgical drainage as the initial approach for(peri)pancreatic fluid collections(PFC)s,while complications associated with endoscopic stent implantation are *** To introduce a novel endoscopic therapy named endoscopic transgastric fenestration(ETGF),which involves resection of tissue by endoscopic accessory between gastric and PFCs without stent implantation,and to evaluate its efficacy and safety compared with PCD for the management of PFCs adjacent to the gastric *** Patients diagnosed with PFCs adjacent to the gastric wall and who subsequently received ETGF or PCD were restrospectively *** for intervention were consistent with related *** analyzed patients baseline characteristics,technical and clinical success rate,recurrence and reintervention rate,procedure-related complications and adverse *** Seventy-two eligible patients were retrospectively identified(ETGF=34,PCD=38)from October 2017 to May *** in the ETGF group had a significantly higher clinical success rate than those in the PCD group(97.1 vs 76.3%,P=0.01).There were no statistically significant differences regarding recurrence,reintervention and incidence of complication between the two *** long-term catheter drainage was very common in the PCD *** Compared with PCD,ETGF has a higher clinical success rate in the management of PFCs adjacent to the gastric *** is an alternative effective strategy for the treatment of PFCs adjacent to the gastric wall.

关键词： (Peri)Pancreatic fluid collections Endoscopic transgastric fenestration Percutaneous drainage