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Cdx1和Cdx2基因表达异常与先天性结肠性肠炎的关系

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中华小儿外科杂志 2003年第5期24卷 406-408页

作者：李龙 Vincent Lui mai har sham 谭广北京大学第一医院小儿外科 100034 香港大学玛丽医院小儿外科香港大学生物化学系

目的结肠炎是先天性巨结肠患儿常见的并发症,组织学研究表明巨结肠性肠炎患儿肠粘膜内的内分泌细胞减少和粘膜内分泌型 IgA不足。而Cdx1和Cdx2基因调控结肠粘膜上皮细胞的分化和增殖,本研究的目的是明确Cdx1和Cdx2基因表达与先天性巨... 详细信息

目的结肠炎是先天性巨结肠患儿常见的并发症,组织学研究表明巨结肠性肠炎患儿肠粘膜内的内分泌细胞减少和粘膜内分泌型 IgA不足。而Cdx1和Cdx2基因调控结肠粘膜上皮细胞的分化和增殖,本研究的目的是明确Cdx1和Cdx2基因表达与先天性巨结肠性肠炎之间的关系。方法采用半定量RT-PCR反应和原位杂交方法,11例先天性巨结肠患儿(年龄1～30个月,4例合并结肠炎)的无神经节细胞段、移行段和扩张段的手术切除标本为病例组,12例年龄相符(1～30个月)的非巨结肠患儿的远端结肠标本为对照组,观察Cdx1和Cdx2基因在其中的表达情况。结果Cdx1和Cdx2基因在对比组和先天性巨结肠的结肠粘膜上皮细胞均有表达,但是此二基因在先天性巨结肠性肠炎患儿的标本中表达明显减弱。结论本研究结果表明Cdx1和Cdx2基因表达减弱与先天性巨结肠性肠炎的病因密切相关。

关键词： Cdx1 Cdx2 基因表达异常先天性结肠性肠炎

T淋巴细胞在脓毒症大鼠血清及肺部的分布特征

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中国急救医学 2024年第5期44卷 443-448页

作者：赵继伟陈彤桦麦皓尘陈雪茹张萌中山大学孙逸仙纪念医院全科医学科二科广东广州510000 中山大学孙逸仙纪念医院深汕中心医院全科医学科广东汕尾516621

目的研究T淋巴细胞在脓毒症大鼠血清及肺部的分布特征。方法20只SPF级SD大鼠随机分为假手术组(sham)24 h、sham 48 h、脓毒症组(CLP)24 h和CLP 48 h。采用盲肠结扎穿孔术(cecal ligation and puncture,CLP)建立脓毒症模型。分别在造模... 详细信息

目的研究T淋巴细胞在脓毒症大鼠血清及肺部的分布特征。方法20只SPF级SD大鼠随机分为假手术组(sham)24 h、sham 48 h、脓毒症组(CLP)24 h和CLP 48 h。采用盲肠结扎穿孔术(cecal ligation and puncture,CLP)建立脓毒症模型。分别在造模后24、48 h留取各组大鼠外周血、支气管肺泡灌洗液(bronchoalveolar lavage fluid,BALF),并进行CD3^(+)T及CD4^(+)T淋巴细胞流式检测。留取肺组织苏木精-伊红(HE)染色光镜观察大鼠肺组织病理变化,并行肺损伤病理评分。结果与sham组相应时间点比较,CLP组血清CD3^(+)T及CD4^(+)T淋巴细胞比例在CLP后24 h及48 h均减低(均Psham组和CLP组血清CD3^(+)及CD4^(+)T淋巴细胞在CLP后48 h较24 h均有不同程度增生,但CLP组增生程度较sham组减低(均Psham 24 h比较,CLP 24 h大鼠BALF中CD3^(+)及CD4^(+)T淋巴细胞比例明显减低(均P<0.05);与CLP 24 h比较,CLP 48 h BALF中CD3^(+)及CD4^(+)T淋巴细胞比例明显升高(P<0.05)。病理观察显示,各组大鼠均有不同程度肺损伤改变,CLP 24 h组大鼠肺损伤评分更高(P<0.05),可见大量炎症细胞浸润、肺间质充血水肿、肺泡出血等改变。结论大鼠血清及肺部T淋巴细胞在脓毒症中后期增生被抑制;血清CD3^(+)及CD4^(+)T淋巴细胞水平从一定程度上可以反映肺组织水平;脓毒症肺脏损伤情况越重,BALF中T淋巴细胞增生趋于减低。

关键词：脓毒症大鼠肺泡灌洗液 T淋巴细胞 CD3^(+) CD4^(+)

The Mechanism of Cornus officinalis Total Glycosides and Cornus Polysaccharide on Myocardial Protection in Rats with Acute Myocardial Infarction

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Chinese Medicine 2016年第2期7卷 45-54页

作者： Ke Chen Jianjun Li Kefang Chen Xiangping Hou Huachao mai Xiaojin Xue Department of Traditional Chinese Medicine Sun Yat-sen Memorial Hospital Affiliated to Sun Yat-sen University Guangzhou China

Objective: To investigate effects of Cornus officinalis Total Glycosides (COTG) and Cornus Polysaccharide (CP) on myocardial protection and on expression of mitochondria biogenesis related gene of acute myocardial infarction (AMI) rats, Materials and Methods: Ninety-six SD rats of SPF level were randomly divided into 5 groups: sham operation group, model group, preventive treatment group, COTG treatment group, CP treatment group, and there were 12 cases in each one. By legating the left anterior descending branch of coronary artery method, acute myocardial infarction model was established. The rat of sham operation group and model group was intragastric administered with physiological saline;other groups were given with corresponding drugs. The cardiac function, the myocardial infarct area, the expression of mitochondrial biogenesis genes such as PGC-1α, PGC-1β, NRF-1mRNA and GSK-3β mRNA, GSK-3β Protein Expression were analyzed. Results: The results revealed that compared with model group, myocardial infarction size, LVDs, LVDd, LVESV, LVEDP, and -dp/dt decreased;LVSP increased in preventive treatment group, COTG treatment group, and CP treatment group (p sham operation group, GSK-3 beta mRNA and protein expression increased in model group, preventive treatment group, COTG treatment group, and CP treatment group (p harides can effectively protect myocardial mitochondria of acute myocardial infa

关键词： Cornus officinalis Total Glycosides Cornus Polysaccharide Acute Myocardial Infarction Glycogen Synthase Kinase 3 Beta

丁苯酞预处理对大鼠心肺复苏后脑缺血再灌注损伤的保护作用研究

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中国临床药理学杂志 2020年第15期36卷 2246-2249,2260页

作者：麦叶顾勇海南省中医院急危重症医学海南海口570000 海南省中医院脑病科海南海口570000 南方医科大学南方医院胸外科广东广州510515

目的探究丁苯酞预处理对大鼠心肺复苏后脑缺血再灌注损伤的保护作用。方法将大鼠随机分为假手术组、模型组、对照组和低、中、高剂量实验组,每组12只。于大鼠窒息前10 min,低、中、高剂量实验组分别经股静脉注射丁苯酞0.25,0.50和0.75 ... 详细信息

目的探究丁苯酞预处理对大鼠心肺复苏后脑缺血再灌注损伤的保护作用。方法将大鼠随机分为假手术组、模型组、对照组和低、中、高剂量实验组,每组12只。于大鼠窒息前10 min,低、中、高剂量实验组分别经股静脉注射丁苯酞0.25,0.50和0.75 mg;对照组经股静脉注射1×10^4 U·kg^-1乌司他丁注射液;假手术组和模型组均经股静脉注射等量0.9%NaCl。除假手术组外,其余各组均建立大鼠心肺复苏后脑缺血再灌注损伤模型;假手术组仅进行气管插管及动静脉穿刺。用神经功能缺损评分标准评估大鼠神经功能缺损情况,用Tunel染色观察大鼠脑组织神经细胞凋亡情况,用免疫印迹法检测大鼠脑组织中高迁移率族蛋白1(HMGB-1)、晚期糖基化终产物受体(RAGE)和核因子-κB(NF-κB)蛋白的表达情况。结果低、高剂量实验组和模型组的神经功能缺损评分分别为(42.06±3.75),(55.51±5.24)和(32.52±4.51)分,脑组织神经细胞凋亡率分别为(27.72±1.73)%,(18.17±2.46)%和(38.47±2.47)%,HMGB-1分别为(0.78±0.09),(0.41±0.06)和(0.90±0.15),RAGE分别为(0.81±0.13),(0.61±0.08)和(1.12±0.16),核NF-κB分别为(1.52±0.13),(0.78±0.06)和(2.06±0.19),质NF-κB蛋白分别为(0.13±0.04),(0.26±0.04)和(0.09±0.02),低、高剂量实验组的上述指标与模型组比较,差异均有统计学意义(均P<0.05)。结论丁苯酞预处理对心肺复苏后脑缺血再灌注损伤具有保护作用,其可能是通过抑制HMGB-1/RAGE/NF-κB通路,降低脑神经细胞凋亡来实现。

关键词：丁苯酞心肺复苏高迁移率族蛋白1 晚期糖基化终产物核因子-κB

Zuogui Wan(左归丸) improves trabecular bone microarchitecture in ovariectomy-induced osteoporosis rats by regulating orexin-A and orexin receptors

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Journal of Traditional Chinese Medicine 2021年第6期41卷 927-934页

作者： LIU Feixiang TAN Feng FAN Qiaoling TONG Weiwei TENG Zhanli YE Sumin LI Xing ZHANG Mingyue CHAI Yi mai Chongying School of Chinese Medicine School of Integrated Chinese and Western MedicineNanjing University of Traditional MedicineNanjing 210023China

OBJECTIVE:To investigate the protective effects of Zuogui Wan(左归丸,ZGW)on bone loss induced by ovariectomy(OVX)and its mechanism via orexin-A and orexin receptors in the osteoporosis rat ***:Fifty Sprague-Dawley female rats were randomly divided into sham-operated(sham)group and four OVX *** subjected to sham and OVX were treated with the vehicle(OVX,1 mL/100 g weight,n=10),17β-estradiol(E2,50μg·kg-1·d-1),and ZGW at the doses of 2.3(ZGW-L)and 4.6(ZGW-H)g/kg/day lyophilized powder daily for 3 months,*** serum biochemical parameters of 17β-estrogen(17β-E2),tartrate-resistant acid phosphatase(TRACP-5 b)and bone alkaline phosphatase(BALP)were measured by enzyme-linked immunosorbent ***-eosin staining was used to detect the changes in the morphological structure in *** tomography was used to evaluate the bone mineral density and microarchitecture of the distal *** gene or protein expression of orexin-A,orexin receptor 1(OX1R),orexin receptor 2(OX2R),osteoprotegerin(OPG)and receptor activator of nuclear factor-κB ligand(RANKL)were assayed by either quantitative polymerase chain reaction or Western blot ***:Compared with the OVX group,ZGW could reduce the serum level of TRACP-5b and increased the serum levels of BALP and 17β-E2(P<0.01).Meanwhile,ZGW could prevent bone loss and improved bone trabecular microarchitecture by increasing the trabeculae structure thickness and trabecular number,and arranging the trabeculae structure *** with the OVX group,it was upregulated for the orexin-A and OX2R mRNA or protein expression from the hypothalamus and tibiae,and OPG in the tibiae of ZGW groups(P<0.01,<0.05),while downregulated for the OX1R mRNA and protein expression in the tibiae and hypothalamus and RANKL from the tibiae(P<0.01).CONCLUSION:ZGW exhibited a protective effect for PMOP that may be mediated via orexin-A and orexin receptors regulation.

关键词： osteoporosis,postmenopausal orexin receptors orexins Zuogui Wan

Exploring the effect of acupuncture plus mild hypothermia on miRNA-204 and its target gene expressions in CIRI rat brain tissues based on MAPK signal pathway

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Journal of Acupuncture and Tuina Science 2021年第5期19卷 338-344页

作者： Tang Yu-lan Liu mai-lan Luo Jian Li Nan Zhang Guo-shan Yu Jie Yang Qian-yun Yang Yan-ping Hunan University of Chinese Medicine Changsha 410208China 不详

Objective:To explore the protective mechanism of acupuncture plus mild hypothermia for cerebral ischemia-reperfusion injury(CIRI)by observing the effects of acupuncture plus mild hypothermia on miRNA-204 and its target gene expressions in CIRI rat brain ***:Sixty Sprague-Dawley rats were divided into a blank control group,a sham operation group,a model group,an acupuncture group,a mild hypothermia group and an acup un cture plus mild hypothermia group accordi ng to the random nu mbertable method(n=10).Except for the blank c on trol group an dthe sham operati on group,rats in the other 4 groups received CIRI *** the model was successfully established,rats in the blank control group were bred routinely for 72 h without any interventions;rats in the sham operation group and the model group were bred routinely for 72 h,and only received bin ding without other interve ntions after surgery;rats in the acup un cture group were bred routinely for 72 h,and received acupuncture at Dazhui(GV 14),Baihui(GV 20)and Shuigou(GV 26)after binding;rats in the mild hypothermia group were bred routinely for 72 h,and received mild hypothermia intervention for 72 h after binding;rats in the acupuncture plus mild hypothermia group were bred routinely for 72 h,followed by receiving acupuncture as in the acup uncture group and mild hypothermia therapy as in the mild hypothermia group after bin *** n eurological impairme nt score,cerebral infarcti on area ratio,the expressions of miRNA-204 and its target genes in eluding Map3k8,Ntrk2 and Ppp3rl in the ischemic hippocampus of each group were observed after 72 h of ***:Before intervention,compared with the bgnk control group and the sham operation group,the neurological impairment scores and the infarction area ratios of the modelled rats were statistically significa ntly increased(all P<***)f indicating that the model was *** intervention,compared with the model group,the neurological

关键词： Acupuncture Therapy Reperfusion Injury Hypothermia,Induced Brain Ischemia MicroRNAs Gene Expression Rats

右腹外侧前额叶对高抑郁水平成年人社会情绪调节的作用:一项tDCS研究

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心理学报 2019年第2期51卷 207-215页

作者：张丹丹刘珍莉陈钰买晓琴深圳大学心理与社会学院深圳市情绪与社会认知科学重点实验室深圳518060 中国人民大学心理学系中国人民大学心理学系实验室北京100872

已有的经颅直流电刺激(transcranialdirectcurrentstimulation,t DCS)研究证明,右腹外侧前额叶(right ventrolateralprefrontalcortex,RVLPFC)是社会情绪调节的重要脑区,激活RVLPFC可显著降低人们对社会性负性情绪体验的强度。社会功能受损是抑郁症患者或抑郁倾向人群的重要特征之一。该群体对社会排斥的敏感性高,且对负性社会情绪体验的情绪调节能力降低。在本研究中,我们采用外显的情绪调节任务,研究了高、低抑郁水平的两组成年人被试在RVLPFC接受阳性t DCS后其情绪调节能力的改变。结果表明,虽然采用t DCS激活RVLPFC可帮助被试通过情绪调节(认知重评)减弱负性情绪体验,但高抑郁水平被试的负性情绪强度下降程度明显小于低抑郁水平被试。另外本文还发现,与源于个人的负性情绪相比,tDCS效应对源于社会的负性情绪(即社会排斥)更强。本研究是采用电或磁刺激提高抑郁人群社会情绪调节能力的首次尝试。实验结果表明,高抑郁水平成年人的RVLPFC仅通过单次、短时间(34min)的t DCS激活,其情绪调节能力并未得到显著提升。这提示对抑郁倾向群体或抑郁症患者的干预或治疗需要多次施加t DCS。

关键词：抑郁倾向经颅直流电刺激右腹外侧前额叶社会排斥负性情绪

慢性脑缺血大鼠~1H和^(31)P活体核磁共振波谱分析

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中华老年医学杂志 2005年第2期24卷 137-139页

作者：章军建周艳冯越刘买利武汉大学中南医院神经科 430071 中国科学院武汉物理与数学研究所波谱与原子分子物理国家重点实验室

目的　采用1H和3 1P活体核磁共振波谱 (in vivoMRS)检测大鼠在慢性脑缺血状态下脑组织的生化改变。　方法　采用双侧颈总动脉结扎 (two vesselsocclusion ,2 VO)制备大鼠慢性脑缺血大鼠模型。运用in vivoMRS分析技术进行海马CA1区1H谱... 详细信息

目的　采用1H和3 1P活体核磁共振波谱 (in vivoMRS)检测大鼠在慢性脑缺血状态下脑组织的生化改变。　方法　采用双侧颈总动脉结扎 (two vesselsocclusion ,2 VO)制备大鼠慢性脑缺血大鼠模型。运用in vivoMRS分析技术进行海马CA1区1H谱和全脑3 1P谱检测。　结果　2 VO术后 2个月大鼠海马CA1区活体定域1H谱中未出现乳酸 (Lac)信号 ;N 乙酰天冬氨酸 (NAA)含量无明显改变 ;而全脑3 1P谱示磷酸单酯 (PME)含量升高 ,PME/ [PME +磷酸二酯 (PDE) ]在假手术组中为 0 5 4± 0 0 3,2 VO组中为 0 6 9± 0 0 2 ,两组比较差异有统计学意义 (P <0 0 1) ;β 三磷酸腺苷 (β ATP)和磷酸肌酸 (PCr)含量无明显改变。　结论　慢性脑缺血时大鼠脑部的神经元损伤不明显 ,能量代谢处于代偿状态 ,而细胞膜性结构增加。

关键词：慢性脑缺血大鼠模型活体改变含量 MRS 代偿 PCr) 脑部细胞膜

Overview of researches on central action mechanism of needling Zusanli(ST 36)

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Journal of Acupuncture and Tuina Science 2017年第3期15卷 191-198页

作者：刘灿周芝根李波杨茜芸常小荣周杰陈勤刘迈兰杨燕萍 School of Acupuncture Moxibustion&TuinaHunan University of Chinese MedicineChangsha 410208China The First Hospital of Hunan University of Chinese Medicine Changsha 410007China The Third Affiliated Hospital of Zhejiang Chinese Medical University Hangzhou 310053China 不详

The contents about the central action mechanisms of needling Zusanli（ST 36） were sorted by summarizing the relevant literatures published in the past 10 years, and it was found that： by comparing acupuncture at Zusanli（ST 36）, other acupoints or sham points, most studies showed that Zusanli（ST 36） had relatively specific characteristics in the brain function response; studies on the central action mechanisms of Zusanli（ST 36） were mainly focused on sequelae of cerebral apoplexy, pain, gastrointestinal diseases, weight loss and drug addiction withdrawal and so on; acupuncture manipulations, stimulation methods, individual differences, stimulation quantity, and stimulation duration, etc. could also induce different brain function responses; acupuncture at Zusanli（ST 36） had an after-working effect, also called long-term effect, but with poor repeatability. So, it was suggested that the future studies should focus on the factors that affect the clinical efficacies and experimental results, including the compatibility effects of the acupoints, reproducibility of research results, sample size, acupuncture methods, long-term effects and disease entities.

关键词： Acupuncture Therapy Point Zusanli(ST 36) Central Mechanism Functional Magnetic Resonance Imaging Specificity of Acupoints Research on Acupoints