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Study of the prevalence and association of ocular chlamydial conjunctivitis in women with genital infection by Chlamydia trachomatis, Mycoplasma genitalium and Candida albicans attending outpatient clinic

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International Journal of Ophthalmology(English edition) 2016年第8期9卷 1176-1186页

作者： Rania Abdelmonem Khattab maha Mohssen abdelfattah Microbiology and Immunology Department Faculty of Pharmacy Cairo University Microbiology and Immunology Department Research Institute of Ophthalmology

AIMTo determine the association between chlamydial conjunctivitis and genital infection by Chlamydia trachomatis, Mycoplasma genitalium and Candida albicans, in addition to the possible relationship between cultured bacterial pathogens and oculogenital chlamydial *** study was performed on 100 (50 symptomatic and 50 asymptomatic) women attending the Gynecological and Obstetric outpatient clinic of Alzahra hospital, Alazhar University. Simultaneously a conjunctival swab was taken from these patients. Polymerase chain reaction (PCR) was done on DNA extracted from both vaginal and conjunctival swab samples. Culture for both vaginal and conjunctival swabs was also *** albicans was the predominant organism isolated by culture in 20% and 40% of conjunctival and vaginal swabs respectively. By the PCR method, ocular Chlamydia trachomatis was present in 60% of symptomatic women, while genital Chlamydia trachomatis infection was present in 30% of symptomatic women. The results of this method also indicated that 25/50 (50%) vaginal swabs were positive with PCR for Candida albicans versus 15/50 (30%) were PCR positive in conjunctival swab. Mycoplasma genitalium was present in only 10% of vaginal swabs. Concomitant oculogenital PCR positive results for Chlamydia trachomatis and Candida albicans were 30% and 28% *** Chlamydia trachomatis was associated with genital Chlamydia trachomatis in a high percentage of women followed by Candida albicans. Cultured bacterial organisms do not play a role in enhancement of Chlamydia trachomatis infection.

关键词： Chlamydia trachomatis Mycoplasma genitalium Candida albicans vaginal swabs polymerase chain reaction

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Evaluation of patients with dry eye disease for conjunctival Chlamydia trachomatis and Ureaplasma urealyticum

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International Journal of Ophthalmology(English edition) 2016年第10期9卷 1457-1465页

作者： maha Mohssen abdelfattah Rania Abdelmonem Khattab Magda H.Mahran Ebrahim S.Elborgy Microbiology and Immunology Department Research Institute of Ophthalmology Giza12556 Egypt Microbiology and Immunology Department Faculty of Pharmacy Cairo University Kasr Al-Aini 11562 CairoEgypt Ophthalmology Department Research Institute of Ophthalmology Giza 12556 Egypt

AIM：To determine the possibility of the development of dry eye disease（DED） as a result of persistent infection with Chlamydia trachomatis and Ureaplasma urealyticum in the conjunctiva of ***： This study was conducted on 58 patients of age range 20-50 y,diagnosed with DED confirmed by Schirmer I test and tear breakup *** non-dry eye control group included 27 subjects of the same *** specimens were collected as conjunctival scrapings and swabs divided into three groups： the first used for bacterial culture,the second and third taken to detect Chlamydia trachomatis and Ureaplasma urealyticum by direct fluorescent antibody（DFA） assay and polymerase chain reaction（PCR） method. RESULTS： Chlamydia trachomatis was detected in 65.5% and 76% of DED patients by DFA and PCR methods *** urealyticum was found in 44.8% of DED infected patients using the PCR *** organisms were identified in only 37.9% of DED patients found to be *** subjects had a 22%detection rate of Chlamydia trachomatis by DFA assay versus a 7% detection rate by PCR; while Ureaplasma urealyticum was detected in 3.7% of the controls by PCR *** conjunctival culture revealed that gram positive microorganisms represented 75% of isolates with coagulase negative Staphylococci the most common（50%） followed by Staphylococcus aureus（20%）,whereas gram negative microorganisms occurred in 25% of cases,isolating Moraxella *** the most frequent organism. CONCLUSION： Our results tend to point out that Chlamydia trachomatis and Ureaplasma urealyticum were detected in a moderate percentage of patients with DED,and could be a fair possibility for its *** is more reliable in detecting Chlamydia trachomatis than DFA *** presence of isolated conjunctival bacterial microflora can be of some potential value.

关键词： dry eye disease conjunctiva Chlamydia trachomatis Ureaplasma urealyticum direct fluorescent antibody polymerase chain reaction

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反复使用抗生素患者结膜菌群的变化及耐药模式研究（英文）

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国际眼科杂志 2019年第11期19卷 1828-1833页

作者： Rania Abdelmonem Khattab maha abdelfattah Iman Fahmy 埃及开罗大学药学院微生物与免疫学系11562 眼科研究所微生物科埃及吉萨12556 眼科研究所眼科埃及吉萨12556

目的:观察和分析反复使用抗生素患者结膜菌群的变化。此外,确定这种反复暴露对结膜菌群抵抗模式的影响。方法:研究纳入40例眼科研究所视网膜科患者。所有受试者至少进行了连续4次、每月一次的玻璃体腔注射(IVI),其中一些患者根据需要延... 详细信息

目的:观察和分析反复使用抗生素患者结膜菌群的变化。此外,确定这种反复暴露对结膜菌群抵抗模式的影响。方法:研究纳入40例眼科研究所视网膜科患者。所有受试者至少进行了连续4次、每月一次的玻璃体腔注射(IVI),其中一些患者根据需要延长至6mo或1y。将患者随机分为3组,每组给予氧氟沙星、莫西沙星或头孢他啶任意一种抗生素。使用Kirby-Bauer扩散法对所有细菌进行药敏试验。结果:研究中,以表皮葡萄球菌(51.2%)为主,其次是金黄色葡萄球菌(14%),微球菌(12.8%)和其他凝固酶阴性葡萄球菌(CONS)占13%。结果显示,在4次随访中,与随机接受头孢他啶治疗的患者的基线培养相比,接受头孢他啶治疗的患者眼部表皮葡萄球菌的百分比有所增加。相比之下,与基线相比,金黄色葡萄球菌的百分比没有明显增加。在氟喹诺酮治疗眼中观察到表皮葡萄球菌的百分比从基线增加。与头孢他啶治疗组相比,氟喹诺酮治疗组的金黄色葡萄球菌百分比较基线升高。然而,随着新一代和老一代氟喹诺酮类药物的暴露,眼部菌群组成模式发生了变化。我们注意到,从基线开始,莫西沙星治疗组比氧氟沙星治疗组的表皮葡萄球菌增多。暴露于老一代和新一代氟喹诺酮类药物的金黄色葡萄球菌的模式没有差异。结论:反复使用眼用抗生素不仅会改变正常眼部菌群的组成,还会选择耐药菌株。

关键词：眼部菌群眼用抗生素表皮葡萄球菌耐药性

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Quality Assessment of Drinking Water in Tanta City, Egypt

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Journal of Environmental Science and Engineering(B) 2013年第5期2卷 257-275页

作者： maha abdelfattah khali. Zenhom El-Said Salem Saly Farouk Gheda Moustafa Mohamed E1-Sheekh Botany Department Faculty of Science Tanta University Tanta 31527 Egypt Geology Department Faculty of Science Tanta University Tanta 31527 Egypt

The physico-chemical and biological （algal and bacterial） quality of tap water in Tanta City were elucidated. Samples were collected during December 2011, covering all the water plants and the areal extension of the pipe line network in Tanta. Total dissolved solids content classifies water origin into surface water, shallow and deep groundwater. Fe, Mn and hardness in some samples of groundwater origin are obviously exceeding the WHO （World Health Organization） limit. Mostly, green algae are found （31 species）, followed by diatoms （19 species）, then blue-green algae （13 species）. CCA （Canonical Corresponding Analysis） indicates that green algae are strongly correlated with pH, NH4, alkalinity, Mn and Si; diatoms with EC （electric conductivity）, turbidity, Zn and Si and blue green algae with EC and alkalinity. 50% of samples of surface origin, 80% of samples of both deep and shallow groundwater origins crossed WHO and Egyptian guideline in their content of mean TCC （Total Coliform Count）. CCA estimates strong correlations of TCC with temperature, NH4 and PO4; E. coli with EC; heterotrophic bacteria with turbidity and NO3; Salmonella sp. with Si and SO4 and Pseudomonas aeruginosa with Mn. Finally, the water plants treatment may be not effective and pipes serve as a reservoir for pathogenic microorganisms.

关键词： Water pollution drinking water physicochemical characteristics algae bacteria.

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