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Increased expression of PELP1 in human sperm is correlated with decreased semen quality

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Asian Journal of Andrology 2018年第5期20卷 425-431页

作者： Izabela Skibinska Miroslaw Andrusiewicz Michal Soin magdalena Jendraszak Paulina Urbaniak Piotr Jedrzejczak Malgorzata Kotwicka Department of Cell Biology Faculty of Health Sciences Poznan University of Medical Sciences Rokietnicka 5D Poznan 60-806 Poland Division of Infertility and Reproductive Endocrinology Faculty of Medicine I Poznan University of Medical Sciences Polna 33 Poznan 60-535 Poland.

Proline-, glutamic acid-, and leucine-rich protein 1 （PELP1） is a scaffolding protein involved in both genomic and nongenomic estrogen signal transduction pathways. To date, the role of PELP1 protein has yet to be characterized in human sperm and has not been associated with sperm parameters. To confirm the presence of PELP1 in human sperm, fresh semen samples were obtained from 178 donors. The study was designed to establish both mRNA and protein presence, and protein cellular localization. Additionally, the number of PELP1-positive spermatozoa was analyzed in men with normal and abnormal semen parameters. Sperm parameters were assessed according to the World Health Organization （WHO） 2010 standards. The presence of PELP1 in spermatozoa was investigated using four precise, independent techniques. The qualitative presence of transcripts and protein was assessed using reverse transcription-polymerase chain reaction （RT-PCR） and western blot protocols, respectively. The cellular localization of PELP1 was investigated by immunocytochemistry. Quantitative analysis of PELP1-positive cells was done by flow cytometry. PELP1 mRNA and protein was confirmed in spermatozoa. Immunocytochemical analysis identified the presence of PELP1 in the midpieces of human sperm irrespective of sperm parameters. Becton Dickinson fluorescence-activated cell sorting （FACSCalibur^Tm） analysis revealed a significantly lower number of PELP1-positive cells in males with normal semen parameters versus abnormal samples （42.78% ± 11.77% vs 61.05% ± 21.70%, respectively; P = 0.014）. The assessment of PELP1 may be a time-saving method used to obtain information about sperm quality. The results of our study suggest that PEPL1 may be utilized as an indicator of sperm quality; thereby, PELP1 may be an additional biomarker useful in the evaluation of male infertility.

关键词： male infertility proline glutamic acid- and leucine-rich protein 1 semen parameters sperm quality spermatozoa

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The Bauhaus

The Bauhaus

Mitogen-Activated Protein Kinase 4 Is a Salicylic Acid-Independent Regulator of Growth But Not of Photosynthesis in Arabidopsis

作者： (author.)Droste magdalena

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Molecular Plant 2014年第7期7卷 1151-1166页

作者： Piotr Gawronski Damian Witon Kateryna Vashutin magdalena Bederska Blaiej Betlihski Anna Rusaczonek Stanislaw Karpinski Department of Plant Genetics Breeding and Biotechnology Faculty of Horticulture and Landscape Architecture Warsaw University ofLife Sciences--SGGW Nowoursynowska 159 Warsaw 02-776 Poland Department of Botany Faculty of Agriculture and Biology Warsaw University of Life Sciences--SGGW Nowoursynowska 159 Warsaw02-776 Poland

Mitogen-activated protein kinase （MAPK） pathways regulate signal transduction from different cellular com- partments and from the extracellular environment to the nucleus in all eukaryotes. One of the best-characterized MAPKs in Arabidopsis thaliana is MPK4, which was shown to be a negative regulator of systemic-acquired resistance. The mpk4 mutant accumulates salicylic acid （SA）, possesses constitutive expression of pathogenesis-related （PR） genes, and has an extremely dwarf phenotype. We show that suppression of SA and phylloquinone synthesis in chloroplasts by knocking down the IC51 gene （by crossing it with the icsl mutant） in the mpk4 mutant background did not revert mpk4-impaired growth. However, it did cause changes in the photosynthetic apparatus and severely impaired the quantum yield of pho- tosystem Ih Transmission microscopy analysis revealed that the chloroplasts＇ structure was strongly altered in the mpk4 and mpk4/icsl double mutant. Analysis of reactive oxygen species （ROS）-scavenging enzymes expression showed that suppression of SA and phylloquinone synthesis in the chloroplasts of the mpk4 mutant caused imbalances in ROS homeo- stasis which were more pronounced in mpk4/icsl than in mpk4. Taken together, the presented results strongly suggest that MPK4 is an ROS/hormonal rheostat hub that negatively, in an SA-dependent manner, regulates immune defenses, but at the same time positively regulates photosynthesis, ROS metabolism, and growth. Therefore, we concluded that MPK4 is a complex regulator of chloroplastic retrograde signaling for photosynthesis, growth, and immune defenses in Arabidopsis.

关键词： chloroplast ultrastructure growth and photosynthesis regulation phylloquinone reactive oxygen species(ROS) retrograde signaling and salicylic acid (SA).

High-precision geochronological constraints on the duration of ‘Dinosaur Pompeii’ and the Yixian Formation

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National Science Review 2021年第6期8卷 117-119页

作者： Yuting Zhong magdalena H.Huyskens Qing-Zhu Yin Yaqiong Wang Qiang Ma Yi-Gang Xu State Key Laboratory of Isotope Geochemistry Guangzhou Institute of GeochemistryChinese Academy of Sciences CAS Center for Excellence in Deep Earth Science Department of Earth and Planetary Sciences University of California at Davis State Key Laboratory of Palaeobiology and Stratigraphy Nanjing Institute of Geology and Palaeontology and Center for Excellence in Life and PaleoenvironmentChinese Academy of Sciences State Key Laboratory of Geological Processes and Mineral Resources School of Earth SciencesChina University of Geosciences

The Early Cretaceous Jehol Biota,renowned for its exceptionally wellpreserved volcanic-influenced ecosystem, was buried in lacustrine and occasionally fluvial sediments. This includes, notably, the Huajiying, Yixian and Jiufotang Formations in northern Hebei and western Liaoning and equivalent ash-interbedding sediments in neighboring areas [1–3](Fig. 1). It harbors many evolutionarily significant taxonomies, e.g. feathered dinosaurs,early birds, mammals and flowering plants, representing one of the most diversified terrestrial biotas of the Mesozoic [1,2,4].

关键词： JEHOL BIOTA

The impact of having an oxygen-rich microporous surface in carbon electrodes for high-power aqueous supercapacitors

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Journal of Energy Chemistry 2021年第2期30卷 36-48,I0002页

作者： Servann Herou Maria Crespo Ribadeneyr Philipp Schlee Hui Luo Liviu Cristian Tanase Christine Roβberg magdalena Titirici Chemical Engineering Department Imperial College LondonImperial College RdKensingtonLondon SW72AZUK National Institute of Materials Physics Atomiştilor 405A077125 Măgurele–IlfovRomania Fraunhofer-Zentrum für Chemisch-Biotechnologische Prozesse CBP Am Haupttor06237 LeunaGermany

The growth of electrical transportation is crucially important to mitigate rising climate change concerns regarding materials *** are high-power devices,particularly suitable for public transportation since they can easily store breaking energy due to their high-rate charging ***,they can function with two carbon electrodes,which is an advantage due to the abundance of carbon in biomass and other waste materials(i.e.,plastic waste).Newly developed supercapacitive nanocarbons display extremely narrow micropores(100 kW kg^(-1)),using fourty times less activating agent than traditionnal chemically activated *** also demonstrate that the affinity between the carbon and the electrolyte is of paramount importance to maintain rapid ionic diffusion in narrow ***,this facile synthesis method shows that low-cost and bio-based free-standing electrode materials with reliable supercapacitive performances can be used in electrochemistry.

关键词： Porous carbon materials Lignin Supercapacitors Oxygen groups

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Myosin *** Regulates Protonemal Patterning and Developmental Timing in the Moss Physcomitrella patens

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Molecular Plant 2011年第5期4卷 909-921页

作者： Shu-Zon Wu Julie A. Ritchie Ai-Hong Pan Ralph S. Quatrano magdalena Bezanilla Department of Biology University of Massachusetts Amherst MA 01003 USA Department of Biology Washington University in St Louis St Louis MO 63130 USA

Plants have two classes of myosins. While recent work has focused on class XI myosins showing that myosin XI is responsible for organelle motility and cytoplasmic streaming, much less is known about the role of myosin VIII in plant growth and development. We have used a combination of RNAi and insertional knockouts to probe myosin VIII function in the moss Physcomitrella patens. We isolated Amyo8ABCDE plants demonstrating that myosin VIII is not required for plant viability. However, myosin VIII mutants are smaller than wild-type plants in part due to a defect in cell size. Additionally, Amyo8ABCDE plants produce more side branches and form gametophores much earlier than wild-type plants. In the ab- sence of nutrient media, Amyo8ABCDE plants exhibit significant protonemal patterning defects, including highly curved protonemal filaments, morphologically defective side branches, as well as an increase in the number of branches. Exogenous auxin partially rescues protonemal defects in Amyo8ABCDE plants grown in the absence of nutrients. This result, together with defects in protonemal branching, smaller caulonemal ceils, and accelerated development in the Amyo8ABCDE plants, suggests that myosin VIII is involved in hormone homeostasis in P patens.

关键词： Cell division cell expansion cell morphogenesis cytoskeleton development.

Analytical and numerical studies for seiches in a closed basin with various geometric shapes of longitudinal section

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Theoretical & Applied Mechanics Letters 2021年第3期11卷 151-156页

作者： I.magdalena N.Karima H.Q.Rif’atin Faculty of Mathematics and Natural Sciences Bandung Institute of TechnologyBandungIndonesia

Seiches are long-period standing waves with a unique period called a natural resonant period,during which the phenomenon of resonance *** occurrence of resonance in coastal areas can cause destruction to surrounding natural and man-made *** determining the resonant period of a given basin,we can pinpoint the conditions that allow waves to achieve *** this study,a mathematical model is developed from the shallow water equations to examine seiches and resonances in various types of closed *** developed model is solved analytically using the separation of variables method to determine the seiches’fundamental resonant *** between the analytical solutions and experimental measurements for resonant periods are also *** is shown that the analytical resonant period confirms the experimental data for closed basin of various geometric *** a finite volume method on a staggered grid,the model is solved numerically to simulate the wave profile when resonance phenomenon *** those numerical simulations,we also obtain the fundamental resonant period for each basin which agrees with the derived analytical period.

关键词： Seiches Fundamental resonant period Closed basin Shallow water equations Finite volume method

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^(13)C-methacetin breath test reproducibility study reveals persistent CYP1A2 stimulation on repeat examinations

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World Journal of Gastroenterology 2011年第45期17卷 4979-4986页

作者： Anna Kasicka-Jonderko Anna Nita Krzysztof Jonderko magdalena Kamińska Barbara Bońska-Fajfrowska Department of Basic Biomedical Science School of Pharmacy Medical University of Silesia

AIM: To find the most reproducible quantitative parameter of a standard 13C-methacetin breath test (13C-MBT). METHODS: Twenty healthy volunteers (10 female, 10 male) underwent the 13C-MBT after intake of 75 mg 13C-methacetin p.o. on three occasions. Short-and medium-term reproducibility was assessed with paired examinations taken at an interval of 2 and 18 d (medians), respectively. RESULTS: The reproducibility of the 1-h cumulative 13C recovery (AUC0-60), characterized by a coefficient of variation of 10%, appeared to be considerably better than the reproducibility of the maximum momentary 13C recovery or the time of reaching it. Remarkably, as opposed to the short gap between consecutive examinations, the capacity of the liver to handle 13C-methacetin increased slightly but statistically significantly when a repeat dose was administered after two to three *** the AUC0-60, the magnitude of this fixed bias amounted to 7.5%. Neither the time gap between the repeat examinations nor the gender of the subjects affected the 13C-MBT reproducibility. CONCLUSION: 13C-MBT is most reproducibly quantified by the cumulative 13C recovery, but the exactitude thereof may be modestly affected by persistent stimulation of CYP1A2 on repeat examinations.

关键词： CYP1A2 重复性检查试验呼气主战坦克考试评估定量参数

Targeting EZH2 for the treatment of soft tissue sarcomas

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Journal of Cancer Metastasis and Treatment 2021年第1期7卷 226-241页

作者： magdalena Karolak Ian Tracy Janet Shipley ZoëS Walters Translational Epigenomics Team Cancer SciencesFaculty of MedicineUniversity of SouthamptonSouthampton SO166YDUK Sarcoma Molecular Pathology Team Divisions of Molecular Pathology and Cancer TherapeuticsThe Institute of Cancer ResearchLondon SM25NGUK.

Soft tissue sarcomas(STS)are a heterogenous group of rare malignancies of mesenchymal origin,affecting both children and *** majority of STS have a poor prognosis and advanced stage at the time of *** treatments for STS largely constitute tumour resection with chemotherapy and/or radiotherapy,and there has been little significant advancement in the application of novel therapies for treatment of these *** current multimodal approach to therapy often leads to long-term side effects,and for some patients,resistance to cytotoxic agents is associated with local recurrence and/or *** is,therefore,a need for novel therapeutic strategies for the treatment of *** advances in epigenetics have implicated the histone methyltransferase,EZH2,in the development and progression of diseases such as breast cancer,lymphoma and more recently *** we will review the current literature for EZH2 in STS,including high expression of EZH2 in STS and correlation of this with specific features of malignancy(metastasis,histological grade,and prognosis).The effects of targeting EZH2 using RNA interference and small molecule inhibitors will also be reviewed and the potential for the use of EZH2 inhibition in therapeutic strategies for STS patients will be discussed.

关键词： EZH2 epigenetics therapy