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American Journal of Plant Sciences 2015年第9期6卷 1444-1465页

作者： Santhiya Kothandaram Prabhakar Deonikar Mrudhuula Mohan Vyshali venugopal V. A. Shiva Ayyadurai Systems Biology Group International Center for Integrative Systems Cambridge MA USA

An integrative computational, in silico, model of C1 metabolism is developed from molecular pathway systems identified from a recent, comprehensive systematic bioinformatics review of C1 metabolism. C1 metabolism is essential for all organisms to provide one-carbon units for methylation and other types of modifications, as well as for nucleic acid, amino acid, and other biomolecule syntheses. C1 metabolism consists of three important molecular pathway systems: 1) methionine biosynthesis, 2) methylation cycle, and 3) formaldehyde detoxification. Each of the three molecular pathway systems is individually modeled using the CytoSolve?? Collaboratory?, a proven and scalable computational systems biology platform for in silico modeling of complex molecular pathway systems. The individual models predict the temporal behavior of formaldehyde, formate, sarcosine, glutathione (GSH), and many other key biomolecules involved in C1 metabolism, which may be hard to measure experimentally. The individual models are then coupled and integrated dynamically using CytoSolve to produce, to the authors’ knowledge, the first comprehensive computational model of C1 metabolism. In silico modeling of the individual and integrated C1 metabolism models enables the identification of the most sensitive parameters involved in the detoxification of formaldehyde. This integrative model of C1 metabolism, giving its systems-based nature, can likely serve as a platform for: 1) generalized research and study of C1 metabolism, 2) hypothesis generation that motivates focused and specific in vitro and in vivo testing in perhaps a more efficient manner, 3) expanding a systems biology understanding of plant bio-molecular systems by integrating other known molecular pathway systems associated with C1 metabolism, and 4) exploring and testing the potential effects of exogenous inputs on the C1 metabolism system.

关键词： In Silico Modeling C1 Metabolism CytoSolve Computational Systems Biology Bioinformatics Molecular Pathway Formaldehyde Detoxification Maize Methionine Biosynthesis Activated Methyl Cycle Folate-Mediated Pathways

Integrative Modeling of Oxidative Stress and C1 Metabolism Reveals Upregulation of Formaldehyde and Downregulation of Glutathione

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American Journal of Plant Sciences 2015年第9期6卷 1527-1542页

作者： Mrudhuula Mohan Santhiya Kothandaram Vyshali venugopal Prabhakar Deonikar V. A. Shiva Ayyadurai Systems Biology Group International Center for Integrative Systems Cambridge MA USA

This research provides, to the authors’ knowledge, the first integrative model of oxidative stress and C1 metabolism in plants. Increased oxidative stress can cause irreversible damage to photosynthetic components and is harmful to plants. Perturbations at the genetic level may increase oxidative stress and upregulate antioxidant systems in plants. One of the key mechanisms involved in oxidative stress regulation is the ascorbate-glutathione cycle which operates in chloroplasts as well as the mitochondria and is responsible for removal of reactive oxygen species (ROS) generated during photosynthetic operations and respiration. In this research, the complexity of molecular pathway systems of oxidative stress is modeled and then integrated with a previously developed in silico model of C1 metabolism system. This molecular systems integration provides two important results: 1) demonstration of the scalability of the CytoSolve®?Collaboratory™, a computational systems biology platform that allows for modular integration of molecular pathway models, by coupling the in silico model of oxidative stress with the in silico model of C1 metabolism, and 2) derivation of new insights on the effects of oxidative stress on C1 metabolism relative to formaldehyde (HCHO), a toxic molecule, and glutathione (GSH), an important indicator of oxidative homeostasis in living systems. Previous in silico modeling of C1 metabolism, without oxidative stress, observed complete removal of formaldehyde via formaldehyde detoxification pathway and no change in glutathione concentrations. The results from this research of integrative oxidative stress with C1 metabolism, however, demonstrate significant upregulation of formaldehyde concentrations, with concomitant downregulation and depletion of glutathione. Sensitivity analysis indicates that kGSH-HCHO, the rate constant of GSH-HCHO binding, VSHMT, the rate of formation of sarcosine from glycine, and , the rate of superoxide formation signifi

关键词： C1 Metabolism Oxidative Stress Formaldehyde Glutathione Formaldehyde Detoxification Methionine Biosynthesis Methylation Cycle Computational Systems Biology CytoSolve Systems Integration Molecular Pathways

Determination of Lisinopril in Bulk and Pharmaceutical Formulations by Cloud Point Extraction—A Green Method

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American Journal of Analytical Chemistry 2020年第8期11卷 289-300页

作者： V. Mallikarjuna Sarma N. V. S. venugopal L. Giribabu Department of Chemistry P. R. Government College (A) Kakinada Andhrapradesh India Department of Chemistry Institute of Science GITAM University Visakhapatnam Andhra Pradesh India Indian Institute of Chemical Technology Hyderabad Telangana India

A sensitive and eco-friendly method was developed for the spectrophotometric determination of Lisinopril (LSP) in bulk and pharmaceutical formulations by cloud point extraction technique. The method was based on the formation of a blue-colored coordination complex between Lisinopril (LSP) and Cobalt Thiocyanate (CTC) at a suitable pH. The Complex in aqueous medium was extracted into surfactant layer by cloud point extraction using a non-ionic surfactant Triton X-114 and then the surfactant layer was dissolved in a suitable volume of ethanol and the amount of Lisinopril was determined spectrophotometrically at a wavelength of 625 nm. The conditions like concentration of the drug, concentration of CTC and of Triton X-114, PH, etc. were optimized by OFAT (One Factor At a Time) method. The linear range of calibration curve was 1 - 6 μg/ml and the linear regression equation with a correlation coefficient of 0.99996 was y = 0.0021 + 0.084x. Preconcentration and enri

关键词： Lisinopril (LSP) Cobalt Thiocyanate (CTC) Coordination Complex Formation Cloud Pint Extraction (CPE) Spectrophotometry

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妊娠性高血压视网膜变化的临床研究（英文）

国际眼科杂志 2017年第6期17卷 1033-1036页

作者： sahana raviraj manipur pavana acharya kavitha chikkanayakanahalli venugopal lakshmi bomalapura ramamurthy 哈桑医学科学研究所眼科印度哈桑卡纳塔克邦573201

目的:研究妊娠性高血压(PIH)患者视网膜变化的患病率,视网膜变化与疾病严重程度的相关性。方法:横断面研究。共收集2014-12/2015-05妊娠性高血压患者100例。所有患者均行眼底检查。单眼或双眼眼底发生变化视为阳性。妊娠性高血压分级为... 详细信息

目的:研究妊娠性高血压(PIH)患者视网膜变化的患病率,视网膜变化与疾病严重程度的相关性。方法:横断面研究。共收集2014-12/2015-05妊娠性高血压患者100例。所有患者均行眼底检查。单眼或双眼眼底发生变化视为阳性。妊娠性高血压分级为轻度子痫前期(血压>140/90~160/90 mmHg),重度子痫前期(血压>160/90 mmHg)和子痫前期(重度子痫前期和抽搐)。结果:患者平均年龄为23.05(19~34)岁。妊娠期为28~41wk。其中62例初产妇以及38例经产妇。轻度子痫前期,重度子痫前期和子痫前期患者占比分别为62%,26%,12%。8例患者发生视网膜变化。观察得出:高血压性视网膜病变Ⅰ级(1%),Ⅱ级(0%),Ⅲ级(2%),Ⅳ级(2%),浆液性视网膜脱离(1%)和中心性浆液性脉络膜视网膜病变(2%)。视网膜变化与PIH严重程度呈正相关,有统计学意义(P=0.0001)。结论:8%的PIH患者视网膜发生变化,视网膜变化与PIH严重程度呈正相关。

关键词：妊娠性高血压视网膜变化高血压性视网膜病变

Studies on the Determination of Compressive Strengths of Different Grades of Rice Husk Ash—An Ecofriendly Concrete

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Journal of Environmental Protection 2013年第4期4卷 329-332页

作者： Patnaikuni Chandan Kumar Nutulapati V. S. venugopal Palli Malleswara Rao Department of Civil Engineering GITAM University Visakhapatnam India

Rice Husk Ash (RHA) Concrete is an eco friendly concrete and has evolved as an innovative technology, capable of achieving the status of being an outstanding advancement in the sphere of concrete technology. The utilization of Rice Husk Ash (RHA) will reduce the dumping of rice husk as well as reduce the construction cost. In this communication investigations for the determination of residual compressive strength of M30 & 40 grades of RHA concretes exposed at different temperatures were carried. Almost all specimens of M30 & 40 grades of RHA concrete and normal concrete exhibited zero strength at a temperature of 1000°C. It shows that concrete cannot withstand a temperature of 1000°C and above. It was also concluded that compressive strength of RHA concrete was higher at °emperature below 500°C and decreases above 500°C.

关键词： Rice Husk Ash Compressive Strength Burning

Evaluation of eco-friendly coagulant from <i>Trigonella foenum-graecum</i>seed

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Advances in Biological Chemistry 2012年第1期2卷 58-63页

作者： Chitteti Ramamurthy Malige Uma Maheswari Natarajan Selvaganabathy Muthuvel Suresh Kumar venugopal Sujatha Chinnasamy Thirunavukkarasu Centre for Advanced Studies in Bioinformatics School of Life Sciences Pondicherry University Puducherry India Department of Biochemistry and Molecular Biology School of Life Sciences Pondicherry University Puducherry India Department of Chemistry Periyar University Salem India

The ability of seed extracts of Trigonella foenum-graecum (T. foenum-graecum) and Cuminum cyminum (C. cyminum) to act as natural coagulants was tested using natural turbid water. Seed extracts were prepared using distilled water and NaCl (0.5 M and 1.0 M) solution. Only 1.0 M NaCl extract of T. foenum-graecum had coagulation capability and did not depend on pH values. Further it showed that natural coagulant obtained from T. foenum-graecum is temperature (upto 100?C) and pH stable (pH 4.0 - 10.0). Extract of C. cyminum had very minimal (16 ± 2) coagulation property. The seed extract of T. foenum-graecum showed about 80% coagulation properties, where as the best known natural coagulants such as Strychnos potatorum and Moringa oleifera, and chemical coagulant such as Al2(SO4)3 showed around 90%, 65% and 95% respectively, which are used as standards for the present study. When compared with pond water, T. foenum-graecum extract treated water shows decrease in alkalinity, turbidity, KMnO4 demand and total coli-form. This study reveals that seed extract of T. foenum-graecum can be used as natural water coagulant.

关键词： Natural Coagulants Water Purifier T. foenum-graecum Fenugreek

Free fatty acid-induced miR-22 inhibits gluconeogenesis via SIRT-1-mediated PGC-1α expression in nonalcoholic fatty liver disease

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iLIVER 2023年第1期2卷 1-9页

作者： Ajay K.Yadav Teja Naveen Sata Daksh Verma Amrendra K.Sah Amit K.Mishra Mrinalini Md.Musa Hossain Kishor Pant Senthil K.venugopal Faculty of Life Sciences and Biotechnology South Asian UniversityAkbar BhawanChanakyapuriNew Delhi 110021India The Hormel Institute University of MinnesotaAustinMN55912 USA

Background and aims:Free fatty acids(FFAs)are one of the important regulators of the progression of nonalcoholic fatty liver *** FFAs are shown to modulate the metabolic status of the liver by modulating several cellular pathways in ***,we elucidated the role of miR-22 in modulating FFAs-mediated ***:Huh7 and WRL68 cells were transfected with nonspecific miRNA,miR-22 premiRs or anti-miR-22 oligos followed by incubation with palmitic acid,oleic acid,and linoleic acid(300μM each)for 48 and 72 h after *** expression of miR-22 was performed using real-time polymerase chain reaction and Western blots were performed for SIRT-1,PGC-1α,PEPCK,and *** groups of C57BL/6 mice(6 mice per group)were fed with standard diet,choline sufficient L-amino acid defined diet or choline-deficient L-amino acid defined(CDAA)diet for 6,18,32,or 54 *** content was measured in the *** of miR-22 and the protein expression of gluconeogenic enzymes were analyzed in the tissue ***:Incubation of miR-22-transfected cells with FFAs inhibited the expression of SIRT-1,PGC-1α,PEPCK,and glucose-6-phosphatase,while miR-22 expression was *** changes were reversed when the cells were transfected with anti-miR-22 ***-fed mice showed the significant increase in triglycerides content and miR-22 expression,while there was an inhibition of SIRT-1,PGC-1α,PEPCK,and glucose-6-phosphatase expression in CDAA-fed mice.

关键词： NASH Gluconeogenesis SIRT-1 CSAA CDAA microRNA-22

Effect of Different Storage Periods on Egg Quality Traits of Ducks

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Journal of Life Sciences 2012年第8期6卷 871-873页

作者： Chinnadurai Pandian Arumugam Sundaresan Kamppasamy Sangilimadan Arcot venugopal Omprakash Mannu Babu Rajamanikam Prabakaran Institute of Poultry Production and Management Tamil Nadu veterinary and Animal Sciences University Chennai 600051 lndia Centre for Animal Production Studies Tamil Nadu Veterinary and Animal Sciences University Chennai 600051 India Tamil Nadu Veterinary and Animal Sciences University Chennai 600051 lndia

The present study was conducted in each 20 eggs stored at 65 °F with 75% relative humidity, for a period of 5 weeks （T-I） 4 weeks （T-2）, 3 weeks （T-3）, 2 weeks （T-4） and 1 week （T-5） with broad end up. The external qualities of eggs like egg weight, length, width and volume were measured. After recording the external qualities the eggs were broken on a glass plate for studying the internal qualities. Storage period had no statistically significant effect on egg weight （g）, egg volume （%）, shell thickness （mm）, shell weight （g） and shell percentage. Egg weight values determined in this present study was between 61.10 and 64.91 g. The egg volume （%）, egg shell thickness （mm）, shell weight （g） and shell percentage for different storage periods were 87.46 and 98.52, 0.28 and 0.34 5.29 and 5.98; and 8.16 and 9.44 respectively. Yolk weight （g）, yolk percentage and albumen percentage was significantly affected （P 〈 0.01） by storage period and the values ranging between 32.20 and 36.40, 48.59 and 58.53 and 32.01 and 43.24 respectively. Storage period had no statistically significant effects on egg shape index and specific gravity. The storage periods on yolk index, yolk colour, albumen index and Haugh unit were statistically significant （P 〈 0.01）. Yolk index value decreased significantly when storage period was lengthened. This study concluded that yolk index, yolk colour, albumen index, albumen percentage and Haugh unit were decreased with increase in storage time.

关键词： Duck egg storage period quality.

Sequential disruption of ALV host receptor genes reveals no sharing of receptors between ALV subgroups A, B, and J

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Journal of Animal Science and Biotechnology 2019年第2期10卷 410-423页

作者： Hong Jo Lee Kyung Je Park Kyung Youn Lee Yongxiu Yao venugopal Nair Jae Yong Han Sciences and Research Institute of Agriculture and Life SciencesSeoul National UniversitySeoul 08826Korea The Pirbright Institute WokingSurrey GU24 0NFUK.

Background: Previously, we showed that targeted disruption of viral receptor genes in avian leukosis virus(ALV)subgroups using clustered regularly interspaced short palindromic repeats(CRISPR)/CRISPR-associated protein 9(Cas9))-based genome editing confers resistance to ALV subgroups B and J. Here, we used the same strategy to target the receptor expressed by ALV subgroup A(TVA) and generate chicken cells resistant to infection by this ***: CRISPR/Cas9-based disruption of exon 2 within the tva gene of DF-1 fibroblasts conferred resistance to infection by ALV subgroup A regardless of whether frameshift mutations were introduced during editing. Conversely,overexpression of the wild-type TVA receptor(wtTVA) by tva-modified DF-1 clones restored susceptibility to ALV subgroup A. The results confirm that exon 2, which contains the low-density lipoprotein receptor class A domain of TVA, is critical for virus entry. Furthermore, we sequentially modified DF-1 cells by editing the tva, tvb, and Na^+/H^+ exchange 1(chNHE1) genes, which are the specific receptors for ALV subgroups A, B, and J, ***: Simultaneous editing of multiple receptors to block infection by different subgroups of ALV confirmed that ALV subgroups A, B, and J do not share host receptors. This strategy could be used to generate cells resistant to multiple viral pathogens that use distinct receptors for cell entry.

关键词： Avian leukosis virus CRISPR/Cas9 Genome editing Host receptor TVA