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The Motor Kinesin 4II Is Important for Growth and Chloroplast Light Avoidance in the Moss <i>Physcomitrella patens</i>

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American Journal of Plant Sciences 2017年第4期8卷 791-809页

作者： Daniel macVeigh-Fierro Erkan Tüzel Luis Vidali Department of Biology and Biotechnology Worcester Polytechnic Institute Worcester MA USA Department of Physics Worcester Polytechnic Institute Worcester MA USA

Understanding how plant cells adapt dynamically to changes in the environment is a fundamental problem of plant biology. Under many conditions, plant cells respond to environmental changes by modifying their intracellular organization. A critical example of intracellular reorganization is chloroplast photo-relocation, which is required for optimal energy harvesting and avoiding photodamage. A key system responsible for the spatial organization of intracellular components is the microtubule cytoskeleton and its associated motor proteins, kinesins. Here we tested the hypothesis that members of the kinesin 4II subfamily are important for chloroplast photo-relocation in the moss Physcomitrella patens. Most land plants, including P. patens, use an actin cytoskeleton-dependent mechanism to transport chloroplasts in response to light. In addition to the actin-based system, P. patens can also transport chloroplasts via a microtubule-dependent mechanism, which is absent in flowering plants. Here, we used a P. patens line that contains an inducible RNAi system to silence all three kinesin 4-II genes present in this moss and evaluated their participation in the microtubule-dependent chloroplast light avoidance response. Because we found a significant effect on cell growth when kinesin 4IIs are silenced, we took advantage of the inducible system to establish a reproducible and quantitative assay to evaluate chloroplast photo-relocation in full-grown cells. Using a laser scanning confocal-based chloroplast light avoidance response assay, we found a reduction in chloroplast motility when kinesin 4IIs were silenced. Hence, in addition to identifying a role for kinesin 4II proteins in protonemal cell growth, our results strongly support the hypothesis that these kinesins play an important role in the chloroplast light avoidance response.

关键词： Microtubules Cytoskeleton Chloroplast Motility Polarization Photo-Relocation

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Characteristics of recent temperate glacier fluctuations in the Parlung Zangbo River basin,southeast Tibetan Plateau

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Chinese Science Bulletin 2010年第20期55卷 2097-2102页

作者： YANG Wei YAO TanDong XU BaiQing ma LingLong WANG ZhiHui WAN Ming Key Laboratory of Tibetan Environment Changes and Land Surface Processes Institute of Tibetan Plateau Research Chinese Academy of Sciences Beijing 100085 China State Key Laboratory of Cryosphere Chinese Academy of Sciences Lanzhou 730000 China Beibei Meteorological Bureau Chongqing 400700 China

Little is known about recent variation of temperate glaciers on the Tibetan Plateau,although they are of particular theoretical and practical interests in terms of climate change and water *** study of glacier mass balance and terminus variation for six temperate glaciers in the Parlung Zangbo River basin,southeast Tibetan Plateau,shows the facts of the overall negative mass balance and the continued terminus retreat from 2005/2006 to 2007/*** mass balances of smaller glaciers were more negative compared to larger *** the trend of glacier mass balance in the Hengduan Mountains,the Himalayas and glacier shrinkage in southeast Tibetan Plateau,the mass loss and recession of temperate glacier in this region are expected if the current climate condition continues.

关键词：青藏高原东南部冰川波动流域冰川物质平衡特征喜马拉雅山脉水资源供应西藏高原

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Hierarchical peony-like FeCo-NC with conductive network and highly active sites as efficient electrocatalyst for rechargeable Zn-air battery

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Nano Research 2020年第4期13卷 1090-1099页

作者： Yiyan Wang Anuj Kumar mang ma Yin Jia Yu Wang Ying Zhang Guoxin Zhang Xiaoming Sun Zifeng Yan State Key Laboratory of Heavy Oil Processing China University of Petroleum(East China)Qingdao 266580China Department of Electrical Engineering and Automation Shandong University of Science and TechnologyQingdao 266590China Department of Chemistry Institute of Humanities and applied ScienceGLA UniversityMathura-281406India

Carbon materials featuring hierarchical pores and atomically dispersed metal sites are promising catalysts for energy storage and conversion ***,we developed a facile strategy to construct functional carbon materials with a fluffy peony-like structure and dense binary FeCo-Nx active sites(termed as f-FeCo-CNT).By regulating the metal content in precursors,a three-dimensional(3D)interconnected conductive carbon nanotubes network was in-situ formed throughout the atomically dispersed FeCo-NC matrix during *** advantage of rich pore hierarchy and co-existence of highly active FeCo-Nx sites and beneficial FeCo alloy nanoparticles,the f-FeCo-CNT material exhibited excellent bifunctional performance towards oxygen reduction reaction/oxygen evolution reactions(ORR/OER)with respect to the atomically dispersed FeCo-NC(SA-f-FeCo-NC)and commercial Pt/C+Ru02 mixture,surpassing the SA-f-FeCo-NC with a 20 mV higher ORR half-wave potential and a 100 mV lower OER overpotential(at 10.0 ma/cm^2).Remarkably,the f-FeCo-CNT-assembled Zn-air battery(ZAB)possessed a maximum specific power of 195.8 mW/cm^2,excellent rate capability,and very good cycling stability at large current density of 20.0 ma/cm^*** work provides a facile and feasible synthetic strategy of constructing low-cost cathode materials with excellent comprehensive ZAB performance.

关键词： atom ically dispersed catalyst hierarchical structure carbon nanotube oxygen electrocatalyst rechargeable zinc-air battery

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Insulin glargine 300 Units/mL Effectiveness in Patients with T2DM Uncontrolled by Basal Insulin in Real-Life Settings in the Czech Republic

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Journal of Diabetes Mellitus 2020年第3期10卷 109-123页

作者： martin Prázný Milan Flekač Petr Jelínek Jana mašková Department of Internal Medicine General Faculty Hospital Prague Czech Republic Faculty of Medicine Charles University Prague Czech Republic Sanofi Prague Czech Republic NEOX Clinical Research Prague Czech Republic

Introduction: To evaluate the clinical effectiveness of gla-300 units/mL (gla-300) in the treatment of patients with type 2 diabetes (T2DM) uncontrolled by basal insulin in real-life clinical settings in the Czech Rep... 详细信息

Introduction: To evaluate the clinical effectiveness of Gla-300 units/mL (Gla-300) in the treatment of patients with type 2 diabetes (T2DM) uncontrolled by basal insulin in real-life clinical settings in the Czech Republic (TOPAZ study). Methods: TOPAZ was a prospective, multi-center, non-interventional, 6-month study. Of the 312 patients screened, 289 were evaluated at month 6. The primary objective was the change of HbA1c after 6 months. The proportion of patients with HbA1c Results: HbA1c decreased significantly after 6 months (mean change 0.9% ± 1.1% DCCT [−9.9 ± 11.6 mmol/mol], p −1.8 ± 3.1 mmol/L) as well as the incidence of hypoglycemia decreased by 49% (p 0.0001) while no weight gain was observed. No significant safety signals were identified.

关键词： Fasting Plasma Glucose Glycated Hemoglobin Hypoglycemia Insulin glargine (gla-300 Units/Ml) Real Life Data Type 2 Diabetes (T2DM)

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整体性分析和逆向邓氏灰色关联分析模型

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Journal of Southeast University(English Edition) 2023年第3期39卷 248-255页

作者：王文平马政东南大学经济管理学院南京211189 东南大学国家发展与政策研究院南京211189 中国电信研究院战略发展研究所上海200122

为了从整体上识别和量化系统中的逆向关联关系,建立了一个逆向邓氏灰色关联分析模型.基于灰关联四公理,构建包含逆向关联关系的公理体系;引入关联方向向量,建立逆向邓氏灰色关联分析模型,并采用遗传算法识别因子的关联方向;以江苏创新... 详细信息

为了从整体上识别和量化系统中的逆向关联关系,建立了一个逆向邓氏灰色关联分析模型.基于灰关联四公理,构建包含逆向关联关系的公理体系;引入关联方向向量,建立逆向邓氏灰色关联分析模型,并采用遗传算法识别因子的关联方向;以江苏创新链质量的关键影响因素识别为例,检验所提出模型的合理性与有效性.案例分析结果表明,逆向邓氏灰色关联分析模型将过程特性下的知识创新环节识别为江苏创新链质量的逆向关联因素,且关联度为-0.815.对比其他4种典型灰色关联分析模型,其对关联方向和强度的判断更加合理.所提出模型在保证整体性分析的同时,可以有效识别并量化逆向关联关系,拓宽了邓氏灰关联分析模型的应用范围.

关键词：逆向邓氏灰色关联分析灰色关联公理灰色关联度遗传算法

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Evaluation of trabecular meshwork-specific promoters in vitro and in vivo using scAAV2 vectors expressing C3 transferase

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International Journal of Ophthalmology(English edition) 2023年第8期16卷 1196-1209页

作者： Jun-Kai Tan Ying Xiao Guo Liu Long-Xiang Huang Wen-Hao ma Yan Xia Xi-Zhen Wang Xian-Jun Zhu Su-Ping Cai Xiao-Bing Wu Yun Wang Xu-Yang Liu Xiamen Eye Center Xiamen UniversityXiamen 361004Fujian ProvinceChina Department of Pathology Hospital of Chengdu University of Traditional Chinese MedicineChengdu 610032Sichuan ProvinceChina Sichuan Provincial Key Laboratory for Human Disease Gene Study Sichuan Provincial People’s HospitalUniversity of Electronic Science and Technology of ChinaChengdu 610072Sichuan ProvinceChina The First Affiliated Hospital of Fujian Medical University Fuzhou 350004Fujian ProvinceChina Beijing FivePlus Molecular Medicine Institute Co. Ltd.Beijing 102600China Shenzhen Key Laboratory of Ophthalmology Shenzhen Eye HospitalSchool of OptometryJinan UniversityShenzhen 518040Guangdong ProvinceChina Department of Ophthalmology Shenzhen People’s Hospitalthe 2nd Clinical Medical CollegeJinan UniversityShenzhen 518020Guangdong ProvinceChina

AIM:To evaluate the potential of two trabecular meshwork(TM)-specific promoters,Chitinase 3-like 1(Ch3L1)and matrix gla protein(MGP),for improving specificity and safety in glaucoma gene therapy based on self-complementary AAV2(scAAV2)vector ***:An scAAV2 vector with C3 transferase(C3)as the reporter gene(scAAV2-C3)was *** scAAV2-C3 vectors were driven by Ch3L1(scAAV2-Ch3L1-C3),MGP(scAAV2-MGP-C3),enhanced MGP(scAAV2-eMGP-C3)and cytomegalovirus(scAAV2-CMV-C3),*** cultured primary human TM cells were treated with each vector at different multiplicities of *** in cell morphology were observed by phase contrast *** stress fibers and Rho GTPases/Rho-associated protein kinase pathway-related molecules were assessed by immunofluorescence staining,real-time quantitative polymerase chain reaction and Western *** vector was injected intracamerally into the one eye of each rat at low and high doses *** vivo green fluorescence was visualized by a Micron III Retinal Imaging *** pressure(IOP)was monitored using a rebound *** responses were evaluated by slit-lamp *** histopathology analysis was examined by hematoxylin and eosin ***:In TM cell culture studies,the vectormediated C3 expression induced morphologic changes,disruption of actin cytoskeleton and reduction of fibronectin expression in TM cells by inhibiting the Rho GTPases/Rhoassociated protein kinase signaling *** the same dose,these changes were significant in TM cells treated with scAAV2-CMV-C3 or scAAV2-Ch3L1-C3,but not in cells treated with scAAV2-eMGP-C3 or *** lowinjected dose,the IOP was significantly decreased in the scAAV2-Ch3L1-C3-injected eyes but not in scAAV2-MGPC3-injected and scAAV2-eMGP-C3-injected *** highinjected dose,significant IOP reduction was observed in the scAAV2-eMGP-C3-injected eyes but not in scAAV2-MGP-C3-injected *** t

关键词： self-complementary AAV2 chitinase 3-like 1 matrix gla protein trabecular meshwork C3 transferase

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江苏省碳达峰路径及其对能源与水资源影响的研究

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长江流域资源与环境 2023年第10期32卷 2147-2157页

作者：周元春马姝魏彬文婷南京财经大学经济学院江苏南京210023 南京财经大学绿色经济发展研究院江苏南京210023 南京财经大学工商管理学院江苏南京210023

作为温室气体排放量高且受气候变化影响大的经济大省,江苏省赶在全国之前实现碳达峰是其应承担的责任和义务。基于LEAP和WEAP模型,构建了自下而上的江苏省能源与水资源耦合模型,探索了江苏省潜在的碳达峰路径以及不同路径选择对能源和... 详细信息

作为温室气体排放量高且受气候变化影响大的经济大省,江苏省赶在全国之前实现碳达峰是其应承担的责任和义务。基于LEAP和WEAP模型,构建了自下而上的江苏省能源与水资源耦合模型,探索了江苏省潜在的碳达峰路径以及不同路径选择对能源和水资源的影响。结果显示:(1)在产业结构调整、能源强度降低、推广清洁能源使用以及电力结构优化的综合政策情景下,江苏省可于2028年实现快速碳达峰,主要减排贡献来自于工业和交通运输业。(2)产业结构调整和能源强度降低可带来4380万t标煤的节能效应。(3)气候变化将会加剧水资源在不同部门间的竞争性使用,而产业结构调整、能源强度降低、推广清洁能源使用和电力结构优化等措施可以缓解不同部门的水资源压力,快速达峰情景可以带来11.8%的节水效应。

关键词：碳达峰协同管理 LEAP-WEAP-JS模型情景分析

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“道德式”凝视对旅游者信用行为的影响机制——基于启动效应的探索性研究

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旅游学刊 2022年第5期37卷 96-109页

作者：张秋实宋瑞马思远中国社会科学院大学研究生院北京102488 中国社会科学院财经战略研究院北京100006 埃克塞特大学商学院英国英格兰埃克塞特C043 SQ

旅游者通过凝视构建和消费旅游目的地,与此同时,其行为举止也暴露在他人的凝视当中,他人可借助“道德式”凝视对旅游者的行为表达无声的评论。在相互凝视过程中,主客体的转变使得权力关系也随之发生变化。文章基于被凝视方的视角,运用... 详细信息

旅游者通过凝视构建和消费旅游目的地,与此同时,其行为举止也暴露在他人的凝视当中,他人可借助“道德式”凝视对旅游者的行为表达无声的评论。在相互凝视过程中,主客体的转变使得权力关系也随之发生变化。文章基于被凝视方的视角,运用实验方法探究“道德式”凝视对旅游者信用行为的影响,研究发现旅游者受到“道德式”凝视后,其公众自我意识和自我问责心理会被唤醒,声誉管理和规范激活机制促使旅游者有意识地内化失信行为意向或修正失信行为。文章是将社会心理学中的启动效应引入对旅游者行为的实证尝试,希望拓展旅游道德伦理学中“旅游凝视”相关理论的维度,为管理和规范旅游者行为提供理论根据。

关键词： “道德式”凝视信用行为公众自我意识自我问责启动效应

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老年糖尿病女性骨质健康状况及干预情况分析

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中国骨质疏松杂志 2012年第12期18卷 1109-1111页

作者：马艳芬刘薇首都医科大学大兴医院专家组北京102600 北京同仁医院内分泌科

目的探讨60岁以上老年女性2型糖尿病(type 2 diabetes mellitus,T2DM)患者与非糖尿病患者骨量、骨代谢标志物骨钙素和骨碱性磷酸酶和血清钙、血清磷及其他骨量相关指标的差异,对糖尿病骨病目前的治疗状况进行分析,为糖尿病骨量异常的治... 详细信息

目的探讨60岁以上老年女性2型糖尿病(type 2 diabetes mellitus,T2DM)患者与非糖尿病患者骨量、骨代谢标志物骨钙素和骨碱性磷酸酶和血清钙、血清磷及其他骨量相关指标的差异,对糖尿病骨病目前的治疗状况进行分析,为糖尿病骨量异常的治疗提供依据。方法用超声测量40例60岁以上老年女性非糖尿病患者及46例老年女性糖尿病患者的跟骨骨密度,并检测两组人群的血清钙、血清无机磷、骨质疏松生化标志物骨钙素和骨碱性磷酸酶。结果糖尿病组的跟骨骨密度,血清钙均低于非糖尿病人群,骨钙素和骨碱性磷酸酶高于非糖尿病组,二者比较有统计学意义。结论 60岁以上老年女性糖尿病患者骨量丢失显著重于同龄非糖尿病人群,对糖尿病性骨量减低的干预和治疗应给予足够重视,以期尽量减少和避免骨折等严重并发症的发生。

关键词：老年女性 2型糖尿病骨密度骨钙素骨碱磷酶

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Mutation breeding inγ-linolenic acid improvement of Cunninghamella echinulata by Pulsed High magnetic Field

Mutation breeding inγ-linolenic acid improvement of Cunning...

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2013年湖北省暨武汉微生物学会会员代表大会暨学术年会

作者： Adnan.B.Al Hawash Xuejiao Zhang Shue Li Li Tan Fuying ma Xiaoyu Zhang Key Laboratory of Molecular Biophysics of MOECollege of Life Science and Technology Huazhong University of Science and Technology

Spore suspension of an oleaginous fungus Cunninghamella echinulata was exposed under different intensity of pulsed high magnetic field for different times. After mutant,spore suspension was spread on PDA plate.64 mutants were isolated and screened by nile red staining and fluorescence microscopic examination.3 mutants were achieved for further fermentation *** biomass content,oil content andγ-linolenic acid(gla) content of three mutants were higher than that of the *** gla content was improved by 34.4%.So,pulsed high magnetic field mutation is an effective method for oleaginous fungi breeding.

关键词： Pulsed high-intensity magnetic field nile red staining γ-linolenic acid Cunninghamella echinulata

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