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botryosphaeria, Neofusicoccum, Neoscytalidium and Pseudofusicoccum species associated with mango in brazil

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Fungal Diversity 2013年第4期61卷 195-208页

作者： marília W.marques Nelson b.Lima marcos A.de Morais jr sami j.michereff Alan j.L.Phillips marcos P.S.Câmara Departamento de Agronomia Universidade Federal Rural de Pernambuco52171-900 RecifeBrazil Departamento de genética Universidade Federal de Pernambuco50732-970 RecifeBrazil Centro de Recursos Microbiológicos Departamento de Ciências da VidaFaculdade de Ciências e TecnologiaUniversidade Nova de Lisboa2829-516 CaparicaPortugal

Members of the botryosphaeriaceae are well known fungi associated with dieback,canker and fruit rot on various hosts worldwide,including *** aim of this study was identify a large collection of botryosphaeriaceae species associated with dieback and stem-end rot of mango in the semi-arid region of Northeastern brazil,and compare the species in relation to mycelial growth,pathogenicity and virulence.A total of 115 isolates were sampled and based on morphology and DNA sequence data(iTS and EF1-α)seven taxa were identified,namely,botryosphaeria dothidea,***,Fusicoccum fabicercianum,Neofusicoccum parvum,*** ***,Neoscytalidium dimidiatum and Pseudofusicoccum *** and *** were the most commonly isolated species,which represented 37%and 33%of all isolates *** is reported for the first time in association with mango diseases *** were significant differences among the species obtained in this study in relation to optimum temperature for mycelial growth and mycelial growth *** species were pathogenic on mango *** were significant differences in virulence among the species,with *** and *** being the most virulent species,while *** was the least virulent.

关键词： botryosphaeriaceae Dieback Stem-end rot mangifera indica Pathogenicity Virulence Phylogeny

Five Colletotrichum species are responsible for mango anthracnose in northeastern brazil

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Fungal Diversity 2013年第4期61卷 75-88页

作者： Nelson b.Lima marcus Vinicius de A.batista marcos A.De Morais jr ma.ia.a.g.b.rb.sa.sa.i j.michereff Kevin D.Hyde marcos P.S.Câmara Departamento de Agronomia Universidade Federal Rural de Pernambuco52171-900 RecifeBrazil Departamento de genética Universidade Federal de Pernambuco50732-970 RecifeBrazil Embrapa Semiárido 56302-970 PetrolinaBrazil institute of Excellence in Fungal Research Mae Fah Luang UniversityChiang Rai 57100Thailand

Colletotrichum species are the most important and widespread form of decay affecting mango fruit *** this study,Colletotrichum species associated with fruit anthracnose isolated from mango in northeastern brazil were subject to molecular and morphological *** partial sequences of the glyceraldehyde-3-phosphate dehydrogenase gene of 143 Colletotrichum isolates was amplified,as an initial measure of genetic diversity.A subset of 47 isolates,selected to represent the range of genetic diversity and geographic origin,were further sequenced using the partial actin,β-tubulin,calmodulin,glutamine synthetase genes and rDNA-iTS *** multilocus sequence analysis,together with a critical examination of the phenotypic characters,revealed four previously described species(Colletotrichum asianum,Colletotrichum fructicola,Colletotrichum tropicale and Colletotrichum karstii)and one new *** new species is introduced as Colletotrichum dianesei and formally described,illustrated and compared with similar *** *** and *** have previously been reported from mango,while the other species represent the first report associated with the mango fruits *** species are reported for the first time associated with the mango fruits in brazil.

关键词： Multilocus phylogeny Morphology New species Plant disease

Species of Lasiodiplodia associated with mango in brazil

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Fungal Diversity 2013年第4期61卷 181-193页

作者： marília W.marques Nelson b.Lima marcos Antônio de Morais jr maria Angélica g.barbosa breno O.Souza sami j.michereff Alan j.L.Phillips marcos P.S.Câmara Departamento de Agronomia Universidade Federal Rural de Pernambuco52171-900 RecifeBrazil Departamento de genética Universidade Federal de Pernambuco50732-970 RecifeBrazil Embrapa Semi-Árido 56302-970 PetrolinaBrazil Centro de Recursos Microbiológicos Departamento de Ciências da VidaFaculdade de Ciências e TecnologiaUniversidade Nova de Lisboa2829-516 CaparicaPortugal

mango(mangifera indica)is a major tropical fruit species cultivated in *** objective of this study was to identify species of Lasiodiplodia associated with dieback and stem-end rot of mango in the semi-arid region of Northeastern brazil,and compare the species in relation to mycelial growth,pathogenicity and virulence.A total of 120 isolates of Lasiodiplodia were used and identifications were made using a combination of morphology and phylogenetic analysis based on partial translation elongation factor 1-αsequence(EF1-α)and internal transcribed spacers(iTS).The following species were identified:Lasiodiplodia crassispora,***,***,***,***,*** and Lasiodiplodia sp..Lasiodiplodia theobromae was the most frequently isolated species,which represented 41%of all the *** this species had been previously reported on mango in brazil,while the other species represent the first report associated with mango tree diseases in this *** crassispora is reported for the first time associated with mango diseases *** were significant differences in mycelial growth rates among the Lasiodiplodia species and also in the optimum temperature for *** species of Lasiodiplodia were pathogenic on mango *** were significant differences in virulence among the species,wherein *** and Lasiodiplodia *** the most virulent,while the least virulent were ***,***,*** and ***.

关键词： botryosphaeriaceae mangifera indica iTS EF1-α Phylogeny Virulence

Species of Lasiodiplodia associated with papaya stem-end rot in brazil

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Fungal Diversity 2014年第4期67卷 127-141页

作者： mariote S.b.Netto iraíldes P.Assuncao gaus S.A.Lima marília W.marques Waléria g.Lima jean H.A.Monteiro Valdir de Queiroz balbino sami j.michereff Alan j.L.Phillips marcos P.S.Camara Departamento de Agronomia Universidade Federal Rural de Pernambuco52171-900 RecifePernambucoBrazil Centro de Ciencias Agrárias Universidade Federal de Alagoas57100-000 Rio LargoAlagoasBrazil Departamento de genética Universidade Federal de Pernambuco50732-970 RecifeBrazil Centro de Recursos Microbiológicos Departamento de Ciencias da VidaFaculdade de Ciencias e TecnologiaUniversidade Nova de Lisboa2829-516 CaparicaPortugal

This study aims to identify and characterize species of Lasiodiplodia associated with stem-end rot of papaya in six different populations in the Northeast of *** identifications were made using a combination of morphology together with a phylogenetic analysis based on partial translation elongation factor 1-αsequence(EF-1α)and internal transcribed spacers(iTS).Five species of Lasiodiplodia were identified:Lasiodiplodia brasiliense ***.,***,*** ***.,*** and *** *** had previously been reported in papaya,while all the other species are reported for the first time in association with this host in brazil and *** theobromae was the most prevalent *** species of Lasiodiplodia were pathogenic on papaya fruit,with *** being the most virulent.

关键词： botryosphaeriaceae Carica papaya Fruit rot EF1-α iTS Phylogeny Virulence