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Compressive Strength of Metakaolin-Based Geopolymers: Influence of KOH Concentration, Temperature, Time and Relative Humidity

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materials Sciences and Applications 2016年第11期7卷 772-791页

作者： Tania Ariadna Garcí a-Mejí a ma. de Lourdes Chávez-Garcí a Facultad de Quí mica UNAM Universidad Nacional Aut&oacute noma de Mé xico Ciudad de M&eacute xico M&eacute xico

The influence of KOH concentration (8 and 12 M) and curing conditions as temperature (40℃ and 60℃), time (7 and 28 days) and relative humidity (85% and 95% RH), on compressive strength of metakaolin-based geopolymers (MK-based GP) was evaluated. Derived from the experimental design technique, and using a factorial design 2K with two replications in the center point, eighteen experiments were conducted. The results reveal that the best performance conditions of geopolymerization to develop a higher compressive strength of 20 MPa are 12 M KOH to 60℃ and 85% RH at 28 curing days. With these conditions, the value of relative humidity of 85%, promotes high strength compact samples, and a maximum of 42 MPa at 90 days. The results of significant, compressive design of GP showed that KOH concentration and curing relative humidity were the most important factors, followed by curing time and temperature. The GP were characterized by XRD, and their evolution on compression strength was followed by SEM.

关键词： Geopolymers Metakaolin Relative Humidity Potassium Hydroxide Design of Experiments

Virulence factors of Enterococcus strains isolated from patients with inflammatory bowel disease

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World Journal of Gastroenterology 2013年第23期19卷 3562-3572页

作者： Edyta Golińska Anna Tomusiak Tomasz Gosiewski Gra yna Wiecek Agnieszka machul Diana Mikoajczyk magorzata Bulanda Piotr B Heczko magdalena Strus Department of Microbiology Jagiellonian University Medical College31-121 Cracow Poland

AIM: To determine the features of Enterococcus that contribute to the development and maintenance of the inflammatory process in patients with inflammatory bowel disease (IBD). METHODS: Multiplex polymerase chain reaction (PCR) was applied to assess the presence of genes that encode virulence factors [surface aggregating protein (asa1), gelatinase (gelE), cytolysin (cylA), extracellular surface protein (esp) and hyaluronidase (hyl)] in the genomic DNA of 28 strains of Enterococcus isolated from the intestinal tissues of children with IBD (n =16) and of children without IBD (controls; n = 12). Additionally, strains with confirmed presence of the gelE gene were tested by PCR for the presence of quorum sensing genes (fsrA, fsrB, fsrC) that control the gelatinase production. Gelatinase activity was tested on agar plates containing 1.6% gelatin. We also analysed the ability of Enterococcus strains to release and decompose hydrogen peroxide (using Analytical Merckoquant peroxide test strips) and tested their ability to adhere to Caco-2 human gut epithelium cells and form biofilms in vitro. RESULTS: A comparison of the genomes of Enterococcus strains isolated from the inflamed mucosa of patients with IBD with those of the control group showed statistically significant differences in the frequency of theasa1 gene and thegelE gene. Furthermore, the cumulative occurrence of different virulence genes in the genome of a single strain ofEnterococcus isolated from the IBD patient group is greater than in a strain from the control group, although no significant difference was found. Statistically significant differences in the decomposition of hydrogen peroxide and adherence to the Caco-2 epithelial cell line between the strains from the patient group and control group were demonstrated. The results also showed that profuse biofilm production was more frequent amongEnterococcus strains isolated from children with IBD than in control strains. CONCLUSION: Enterococcus strains that a

关键词： Enterococcus Virulence factors Inflammatory bowel disease Hydrogen peroxide Biofilm

Alzheimer’s disease and type 2 diabetes mellitus:Pathophysiologic and pharmacotherapeutics links

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World Journal of Diabetes 2021年第6期12卷 745-766页

作者： Milagros Rojas Mervin Chávez-Castillo Jordan Bautista Ángel Ortega manuel Nava Juan Salazar Edgar Díaz-Camargo Oscar Medina Joselyn Rojas-Quintero Valmore Bermúdez Endocrine and Metabolic Diseases Research Center School of MedicineUniversity of ZuliaMaracaibo 4004Venezuela Universidad Simón Bolívar Facultad de Ciencias Jurídicas y SocialesCúcuta 540006Colombia Pulmonary and Critical Care Medicine Department Brigham and Women's HospitalHarvard Medical SchoolBostonMA 02155United States Universidad Simón Bolívar Facultad de Ciencias de la SaludBarranquilla 080001Colombia

At present,Alzheimer’s disease(AD)and type 2 diabetes mellitus(T2DM)are two highly prevalent disorders worldwide,especially among elderly individuals.T2DM appears to be associated with cognitive dysfunction,with a higher risk of developing neurocognitive disorders,including *** diseases have been observed to share various pathophysiological mechanisms,including alterations in insulin signaling,defects in glucose transporters(GLUTs),and mitochondrial dysfunctions in the ***,the aim of this review is to summarize the current knowledge regarding the molecular mechanisms implicated in the association of these pathologies as well as recent therapeutic *** this context,the hyperphosphorylation of tau and the formation of neurofibrillary tangles have been associated with the dysfunction of the phosphatidylinositol 3-kinase and mitogen-activated protein kinase pathways in the nervous tissues as well as the decrease in the expression of GLUT-1 and GLUT-3 in the different areas of the brain,increase in reactive oxygen species,and production of mitochondrial alterations that occur in *** findings have contributed to the implementation of overlapping pharmacological interventions based on the use of insulin and antidiabetic drugs,or,more recently,azeliragon,amylin,among others,which have shown possible beneficial effects in diabetic patients diagnosed with AD.

关键词： Alzheimer’s disease Type 2 diabetes mellitus Oxidative stress Islet amyloid polypeptide Glucagon-like peptide 1 Cognitive dysfunction Hypoglycemic agents

短发夹状RNA干扰对子宫颈癌细胞中Pin1基因表达及细胞增殖和凋亡的影响

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中华妇产科杂志 2006年第6期41卷 417-421页

作者：李红雨朱涛周金华徐茜王世宣白向阳卢运萍马丁华中科技大学同济医学院附属同济医院肿瘤生物医学中心

目的研究短发夹状RNA(shRNA)干扰对宫颈癌细胞中Pin1基因表达及细胞增殖和凋亡的影响。方法构建靶向Pin1基因的shRNA真核表达质粒pSIREN-Pin1,在脂质体介导下转染人宫颈癌细胞系HeLa细胞(HeLa/p-shRNA组),同时以对照质粒pSIREN-Con(HeLa... 详细信息

目的研究短发夹状RNA(shRNA)干扰对宫颈癌细胞中Pin1基因表达及细胞增殖和凋亡的影响。方法构建靶向Pin1基因的shRNA真核表达质粒pSIREN-Pin1,在脂质体介导下转染人宫颈癌细胞系HeLa细胞(HeLa/p-shRNA组),同时以对照质粒pSIREN-Con(HeLa/p-Con组)和无血清培养基转染HeLa细胞(HeLa组)作为对照,分别应用RT-PCR技术及蛋白印迹法检测Pin1mRNA及蛋白表达水平,四甲基偶氮唑蓝(MTT)比色法和软琼脂细胞克隆实验检测细胞增殖状况,流式细胞仪分析细胞凋亡情况。结果转染后48h,HeLa/p-shRNA组Pin1mRNA及蛋白表达水平分别为0·19±0·05和0·33±0·14,HeLa/p-Con组分别为0·84±0·16和0·79±0·17,HeLa组分别为0·89±0·11和0·81±0·15,前组Pin1mRNA及蛋白表达水平分别与后两组比较,差异均有统计学意义(P<0·05);转染pSIREN-Pin1质粒后HeLa细胞中Pin1mRNA及蛋白表达抑制率分别为77%和58%。MTT比色法检测显示,HeLa/p-shRNA组细胞增殖率明显下降(P<0·05)。软琼脂克隆实验显示,HeLa/p-shRNA组细胞克隆小而稀疏,细胞克隆形成率为(12±3)%,明显低于HeLa/p-Con组的(20±5)%和HeLa组的(24±4)%(P<0·05)。流式细胞仪分析显示,HeLa/p-shRNA组细胞凋亡率为(24·3±5·7)%,明显高于HeLa/p-Con组的(5·0±1·4)%和HeLa组的(1·8±0·4)%(P<0·05)。结论shRNA干扰技术能有效抑制靶基因Pin1的表达,进而可抑制宫颈癌细胞增殖并诱导细胞凋亡增加,为宫颈癌的基因研究及治疗提供新思路。

关键词：基因表达 RNA干扰宫颈肿瘤肽基脯氨酰异构酶细胞凋亡

Implementation of ison iazid preventive therapy in southern Lima, Peru: an analysis of health center characteristics

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Infectious Diseases of Poverty 2021年第3期10卷 26-33页

作者： Roberto Zegarra-Chaponan Lily Victoria Bonadonna Courtney M.Yuen martha Brigida martina-Chavez Jhon Zeladita-Huaman Universidad maria Auxiliadora LimaPeru Wayne State University School of Medicine DetroitMlUSA Harvard Medical School BostonMAUSA. Universidad Nacional mayor de San marcos LimaPeru.

Background:Tuberculosis(TB)prevention through the use of preventive treatment is a critical activity in the elimination *** multiple settings,limited staffing has been identified as a barrier to managing preventive treatment for TB *** study aims to determine how health center staffing,service type,and TB caseload affects implementation of isoniazid preventive therapy(IPT)forTB contacts in southern ***:We conducted an ecological study in 2019 in southern Lima,*** the review of medical records,we identified contacts ofTB patients who initiated IPT during 2016-2018,and who were 0-19 years old,the age group eligible for IPT according to Peruvian *** assessed bivariate associations between health center characteristics(numbers of physicians and nurses,types of services available,annual TB caseload)and IPT initiation and completion using binomial logistic ***:Among 977 contacts,69%took more than a week to start IPT and 41%did not complete *** those who successfully completed IPT,58%did not complete full medical *** was no significant difference in IPT completion or adherence based on whether health centers had more physicians and nurses,more comprehensive services,or higherTB *** contacts,female sex was associated with delay in initiating IPT(P=0.005),age 5-19 years old was associated with completion of IPT(P=0.025)and agema,Peru,but insufficient staffing of health centers may not be *** research is needed to identify how IPT implementation can be improved,potentially through improving stafftraining or monitoring and supervision.

关键词： Contacts Chemotherapy Latent tuberculosis Children Adolescent

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类风湿关节炎生物标记物的研究进展

世界临床药物 2017年第10期38卷 719-720,67+71页

作者：徐小涛王生马春梅郑斌颜仁杰刘莉中国医药工业研究总院上海医药工业研究院药理评价研究中心上海200437 中国医药工业研究总院上海医药工业研究院创新药物与制药工艺国家重点实验室上海200437 上海市生物物质成药性评价专业技术服务平台上海200437

类风湿关节炎(RA)是一种以慢性滑膜炎、关节软骨及骨质破坏、关节畸形及功能丧失等为主要特征的自身免疫性疾病,其病因和发病机制尚不明确,尚无特效的治疗方法。生物标记物作为重要的辅助诊疗指标,已广泛应用于RA的诊断、分型以及预后... 详细信息

类风湿关节炎(RA)是一种以慢性滑膜炎、关节软骨及骨质破坏、关节畸形及功能丧失等为主要特征的自身免疫性疾病,其病因和发病机制尚不明确,尚无特效的治疗方法。生物标记物作为重要的辅助诊疗指标,已广泛应用于RA的诊断、分型以及预后评估等诊疗过程中。蛋白类生物标记物是最早应用于临床的RA诊断标记物,炎性细胞因子水平对RA影响的研究也不断取得进展,近年来肠道菌群与RA的相关性也引起研究者的广泛关注。本文简要综述RA的蛋白类生物标记物、炎性细胞因子与RA的关系以及其他标记物的研究进展,旨在为RA的多途径、多因素早期发现RA,精准治疗RA提供理论参考。

关键词：类风湿关节炎(RA) 蛋白类生物标记物炎性细胞因子肠道菌群

SCREENING AND MOLECULAR CHARACTERIZATION OF FUNGI CAPABLE OF LACCASE PRODUCTION FROM DIFFERENT FOREST ECOSYSTEMS OF MICHOACAN STATE,MEXICO

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SCREENING AND MOLECULAR CHARACTERIZATION OF FUNGI CAPABLE OF...

急性心肌梗死合并肥厚型心肌病患者临床特征和远期预后及危险因素分析

中国菌物学会2016年学术年会

作者： Irum Mukhtar Gerardo Vazquez-marrufo ma.Soledad Vázquez-Garcidue?as marina Arredondo-Santoyo Centro Multidisciplinario de Estudios en Biotecnología CEMB Facultad de Medicina Vterinariay ZootecniaUniversidad Michoacana de San Nicolás de Hidalgo División de Estudios de Posgrado Facultad de Ciencias Médicasy Biológicas “Dr.Ignacio Chávez”Universidad Michoacana de San Nicolás de Hidalgo

The goal of this study was to isolate and molecular identification of basidiomycetes species with laccase activity from soil and vegetative mycelial aggregations in different forest *** species were isolated from soil and mycelia,using different techniques,samples collected from five different geographic regions with dominant vegetation of Pinusspp.,Abiesspp.,and Quercus spp.,in state of Michoacan,*** activity of fungal isolates was analyzed by guaiacol *** number of basidiomycetes with laccase activity were isolated from mycelialagrigations,collected from Atecuaro（pinus and oak forest）,Michoacán,*** of laccase positive fungi was carried out by sequence comparison and phylogenetic analysis of internal transcribed region（ITS1F-5.8S-ITS4B） of r DNA genes with reference taxa.A total of fourteen strains（14） with high laccase activity of basidiomycetes were identified in thirteen genera i.e.,Bjerkandera,Corticiaceae,Coriolopsis,Echinodontium,Ganoderma,Hexagonia,Irpex,Limonomyces,Psathyrella,Peniophora,Phlebiopsis,Trametes and Trichaptum.

关键词： laccase fungi basidomycetes guaiacol phylogenetic ITS region

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中国医药 2018年第12期13卷 1761-1765页

作者：马越刘放张黛刘妍张敬林王德广彭文龙程宇婧赵奇周玉杰首都医科大学附属北京安贞医院心内科北京市心肺血管疾病研究所冠心病精准治疗北京市重点实验室首都医科大学冠心病临床诊疗与研究中心 100029

目的分析急性心肌梗死(AMI)合并肥厚型心肌病(HCM)患者的临床特征、远期预后及相关危险因素。方法连续入选2005年7月至2017年10月在首都医科大学附属北京安贞医院就诊的HCM合并AMI的成年患者78例,按照性别、年龄和入院日期以1:1的比例... 详细信息

目的分析急性心肌梗死(AMI)合并肥厚型心肌病(HCM)患者的临床特征、远期预后及相关危险因素。方法连续入选2005年7月至2017年10月在首都医科大学附属北京安贞医院就诊的HCM合并AMI的成年患者78例,按照性别、年龄和入院日期以1:1的比例匹配单纯AMI组及单纯HCM组,对患者的临床资料进行回顾性分析,包括临床特征、冠状动脉造影检查结果及血运重建情况等。研究终点为主要不良心血管事件(maCE)和其他次要终点事件。结果 HCM合并AMI组左心室射血分数和室间隔厚度均大于单纯AMI组,但均小于单纯HCM组(均P maCE患者19例(27.9%),单纯AMI组11例(16. 7%),单纯HCM组8例(12. 3%)。HCM合并AMI组的远期预后更差,maCE(Log-rank P=0. 030)和次要终点事件(Log-rank P=0.032)发生率均高于其他2组。COX风险比例模型分析显示,远期maCE的危险因素为陈旧性心肌梗死(风险比=5. 873,95%置信区间:1.449～23. 807,P=0. 013)、心力衰竭(风险比=10. 990,95%置信区间:2. 651～45. 570,P=0.001)和冠状动脉累及数目(风险比=86. 343,95%置信区间:4. 138～1801.415,P=0.004)。结论与单纯HCM及单纯AMI患者相比,HCM合并AMI患者的心血管危险因素较多,远期预后较差。陈旧性心肌梗死和心力衰竭对于AMI患者远期maCE的发生有预测价值.而HCM疾病本身不是maCE的危险因素。

关键词：急性心肌梗死肥厚型心肌病远期预后