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c-Myc调控葡萄糖转运蛋白4激活酪氨酸激酶/信号转导与转录激活因子促进三阴性乳腺癌机制研究

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中华实验外科杂志 2024年第4期41卷 674-678页

作者：张莹范静婧吴中语马伟强马斌林新疆医科大学附属肿瘤医院新疆乳腺甲状腺肿瘤临床医学研究中心乌鲁木齐830011

目的探讨c-Myc调控葡萄糖转运蛋白4(GLUT4)激活酪氨酸激酶(JAK)/信号转导与转录激活因子(STAT)信号通路促进三阴性乳腺癌(TNBC)进展机制。方法通过生信分析GLUT4、c-Myc关联性及GLUT4与乳腺癌临床病理特征间关系。收集三阴性乳腺癌患者... 详细信息

目的探讨c-Myc调控葡萄糖转运蛋白4(GLUT4)激活酪氨酸激酶(JAK)/信号转导与转录激活因子(STAT)信号通路促进三阴性乳腺癌(TNBC)进展机制。方法通过生信分析GLUT4、c-Myc关联性及GLUT4与乳腺癌临床病理特征间关系。收集三阴性乳腺癌患者临床样本,免疫组织化学检测GLUT4和c-Myc表达水平。构建敲除c-Myc的MDA-MB-231细胞株,通过实时定量反转录聚合酶链反应(RT-qPCR)及蛋白质印迹法(Western blot)检测GLUT4表达水平。构建敲除GLUT4和同时敲除GLUT4及c-Myc的MDA-MB-231细胞株,细胞计数试剂盒(CCK-8)检测增殖,流式细胞仪检测凋亡,Transwell检测迁移和侵袭。Western blot进一步分析c-Myc调控GLUT4对JAK/STAT的影响。正态分布计量资料比较采用独立样本t检验,多组资料组间比较采用方差分析。结果生信分析结果显示,在乳腺癌中GLUT4明显下调(P<0.01)且与乳腺癌TNM分期、病理分级、PAM50分型等相关。免疫组织化学评分结果显示,c-Myc在TNBC癌组织中表达显著高于癌旁组织(7.233比4.567,t=4.551,P<0.01),而GLUT4显著低表达(4.800比7.933,t=5.702,P<0.01)。在MDA-MB-231细胞中敲除c-Myc,RT-qPCR结果显示,c-Myc敲除组GLUT4表达量高于阴性对照组(2.486±0.273比1.070±0.140,F=55.005,P<0.01),Western blot结果显示,c-Myc敲除组GLUT4表达量高于阴性对照组(0.691±0.041比0.431±0.055,F=29.027,P<0.01)。构建GLUT4低表达的MDA-MB-231细胞株,RT-qPCR结果显示,GLUT4沉默组表达量低于阴性对照组,差异有统计学意义(0.507±0.076/0.137±0.025/0.317±0.085比1.150±0.200,F=45.016,P<0.01)。CCK-8结果显示,组细胞存活率高于阴性对照组(128.614±13.780比95.875±9.130,F=26.927,P<0.01),继续敲除c-Myc后细胞存活率低于阴性对照组(70.977±5.906比95.875±9.130,F=26.927,P<0.01)。流式细胞术结果显示,同时敲除GLUT4及c-Myc后细胞凋亡高于阴性对照组(12.170±1.283比3.263±0.691,F=102.946,P<0.01)。Transwell结果显示,敲除GLUT4后细胞迁移数高于阴性对照组[(237.333±14.364)个比(156.667±10.693)个,F=91.074,P<0.01]、侵袭数高于阴性对照组[(257.000±9.539)个比(152.333±5.132)个,F=90.437,P<0.01],细胞迁移侵袭能力增强,而同时敲除c-Myc和GLUT4后细胞迁移数低于阴性对照组[(67.000±14.177)个比(156.667±10.693)个,F=91.074,P<0.01]、侵袭数低于阴性对照组[(91.667±13.650)个比(152.333±5.132)个,F=90.437,P<0.01],细胞迁移侵袭能力减弱。Western blot结果显示,敲除GLUT4后c-Myc(0.814±0.060比0.447±0.028)、p-JAK2(0.629±0.038比0.380±0.030)、p-STAT3(0.698±0.029比0.288±0.016)表达量均高于相应阴性对照组(F=17.541,P<0.01),而继续敲低c-Myc后,GLUT4表达量高于单纯敲低GLUT4组(0.618±0.029比0.529±0.049,F=68.772,P<0.05),p-JAK2(0.510±0.033比0.629±0.038)、p-STAT3(0.410±0.050比0.698±0.029)表达量低于单纯敲低GLUT4组(F=68.772,P<0.01)。结论在三阴性乳腺癌中,c-Myc可抑制GLUT4表达并通过调控JAK/STAT信号通路影响肿瘤细胞的增殖侵袭和凋亡。

关键词：三阴性乳腺癌 c-Myc 葡萄糖转运蛋白4 酪氨酸激酶/信号转导与转录激活因子信号通路

基于“土虚木郁”探讨腹泻型肠易激综合征与溃疡性结肠炎共同发病机制及证治

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辽宁中医杂志 2024年第4期51卷 54-57页

作者：李璇李中玉马金鑫陈婷王凤云唐旭东北京中医药大学研究生院北京100029 中国中医科学院西苑医院北京100091 北京大学中医药临床医学院北京100091 中国中医科学院北京100700

腹泻型肠易激综合征(IBS-D)与溃疡性结肠炎(UC)是临床较为常见的消化系统疾病,二者虽在疾病性质上有所不同,但在临床症状表现、发病机制及治疗策略等方面有诸多类似之处。基于对中医古籍的研究及临证经验的总结,众多中医学者认为“土虚... 详细信息

腹泻型肠易激综合征(IBS-D)与溃疡性结肠炎(UC)是临床较为常见的消化系统疾病,二者虽在疾病性质上有所不同,但在临床症状表现、发病机制及治疗策略等方面有诸多类似之处。基于对中医古籍的研究及临证经验的总结,众多中医学者认为“土虚木郁”是此二者疾病共同的发病机制,治疗时从肝脾论治往往可取得满意的临床疗效。多项现代医学研究表明中医“土虚木郁”与脑肠互动异常、肠道菌群-胆汁酸轴失衡等IBS-D和UC共同的病理生理学改变密切相关。该文基于中医“异病同治”理念及现代医学研究进展,以“土虚木郁”为切入点,探讨IBS-D和UC共同的发病机制及临床证治。

关键词：腹泻型肠易激综合征溃疡性结肠炎土虚木郁病因病机健脾疏肝

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Single-electron charging and ultrafast dynamics of bimetallic Au_(144−x)Agx(PET)_(60) nanoclusters

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Nano Research 2022年第9期15卷 8573-8578页

作者： Xiangsha Du Hedi ma Xinwen Zhang Meng Zhou zhongyu Liu He Wang Gangli Wang Rongchao Jin Department of Chemistry Carnegie Mellon UniversityPittsburghPennsylvania 15213USA Department of Chemistry Georgia State UniversityAtlantaGeorgia 30302USA Department of Physics University of MiamiCoral GablesFlorida 33146USA Department of Chemical Physics University of Science and Technology of ChinaHefei 230026China

Alloying is an important strategy in tailoring the functionality of *** metal nanoclusters(NCs),the introduction of a heterometal leads to alloy nanoclusters that often outperform the homometal ones in terms of the physical and chemical *** this work,a series of four M_(144)(PET)60 alloy NCs(where,M=Au/Ag,PET=–SCH_(2)CH_(2)Ph)are synthesized and *** silver doping into the homogold template(Au_(144))leads to more prominent optical absorption features in the steady-state spectrum in the visible *** transient absorption spectroscopy reveals the effect of Ag doping on the electronic relaxation dynamics compared to Au_(144)and the pump fluence independent *** results reflect a narrowing of HOMO–LUMO gap(E_(g))induced by Ag doping.A temperature dependence of the single-electron charging is also observed for the series of alloy NCs,in which the E_(g)values of the alloy NCs enlarge as the temperature decreases,which is characteristic of semiconducting behavior.

关键词： 144-atom alloy nanoclusters Ag doping single-electron charging electron dynamics energy gap

超声早期诊断强直性脊柱炎外周附着点病变的作用

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中国现代医生 2021年第16期59卷 121-123,129页

作者：孟庆鑫马忠宇李丹于涛陈芗茹李冬梅牡丹江医学院附属第二医院超声科黑龙江牡丹江157011 牡丹江医学院附属第二医院骨科黑龙江牡丹江157011 牡丹江医学院附属第二医院心电室黑龙江牡丹江157011

目的观察超声早期诊断强直性脊柱炎外周附着点病变的临床价值。方法选取2018年1月至2019年12月我院收治的30例强直性脊柱炎患者作为观察组,并将同期在我院常规体检的20名健康志愿者作为对照组,采用高频超声及能量多普勒对两组受试者双下... 详细信息

目的观察超声早期诊断强直性脊柱炎外周附着点病变的临床价值。方法选取2018年1月至2019年12月我院收治的30例强直性脊柱炎患者作为观察组,并将同期在我院常规体检的20名健康志愿者作为对照组,采用高频超声及能量多普勒对两组受试者双下肢9个附着点进行检测,比较肌腱端异常声像指标。结果30例强直性脊柱炎患者540个外周附着点共有180处(33.3%)存在病变,显著高于对照组的30处(8.3%),差异有统计学意义(P<0.05);最易受累位置为股四头肌肌腱附着点、跟腱以及髌腱止点;骨赘是下肢附着点中最为常见的病变,占12.0%(65/540),其次为滑囊炎,占10.2%(55/540);观察组存在临床表现肌腱附着点均表现为超声阳性;两组右侧冈上肌肌腱止点肌腱厚度比较,差异有统计学意义(P<0.05)。结论超声早期诊断强直性脊柱炎,能够有效检出外周附着点异常病变,能够为临床治疗方案的制订提供可靠的参考,具有较高临床价值。

关键词：超声诊断强直性脊柱炎外周附着点病变骨赘临床价值

Characterization of the copper-containing amine oxidase from Trifolium pratense seedlings

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Nuclear Science and Techniques 2016年第3期27卷 138-144页

作者： Yong-Ming Zhang Guo-Long Li Rui-Bo zhongyu-Xing ma Pei Gong Feng Zhang College of Life Science and Technology Inner Mongolia Normal UniversityHohhot 010022China Sugar beet Physiological Institute Inner Mongolia Agricultural UniversityHohhot 010019China Agricultrual Nanocenter School of Life SciencesInner Mongolia Agricultural UniversityHohhot 010018China

We studied the kinetic characterizations of the Trifolium pratense seedlings copper-containing amine oxidase(TPAO) by using various amine-containing *** catalyzing rate for all of amine-containing substrates can be ordered as diamines > polyamines > aromatic monoamines,and it shows an apparent trend in each category of substrates such as the longer the carbon chain,the lower the V_(max) is,so does the V_(max)/K_m values but is opposite for the K_m value of *** distinct differences between the kinetic parameters for different amine-containing substrates indicated that the rate-determining step of the catalytic reaction strongly depends on the substrate's chemical *** is concluded that both pH and ionic strength can affect the catalytic activity of TPAO via influencing the coulomb interaction-mediated enzyme-substrate docking processes,which can be attributed to the potential of charged groups from both substrates and the activity sites of TPAO by the regulation of ionic strength.

关键词：胺氧化酶含铜幼苗红三叶表征库仑相互作用催化速率离子强度

Behaviors and kinetics of toluene adsorption-desorption on activated carbons with varying pore structure

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Journal of Environmental Sciences 2018年第5期30卷 104-114页

作者： Xi Yang Honghong Yi Xiaolong Tang Shunzheng Zhao zhongyu Yang Yueqiang ma Tiecheng Feng Xiaoxu Cui School of Energy and Environmental Engineering University of Science and Technology BeijingBeijing 100083China Beijing Key Laboratory of Resource-oriented Treatment of industrial Pollutants Beijing 100083China

This work was undertaken to investigate the behaviors and kinetics of toluene adsorption and desorption on activated carbons with varying pore structure. Five kinds of activated carbon from different raw materials were selected. Adsorption isotherms and breakthrough curves for toluene were measured. Langmuir and Freundlich equations were fitted to the equilibrium data, and the Freundlich equation was more suitable for simulating toluene adsorption. The process consisted of monolayer, multilayer and partial active site adsorption types. The effect of the pore structure of the activated carbons on toluene adsorption capacity was investigated. The quasi-first-order model was more suitable for describing the process than the quasi-second-order model. The adsorption data was also modeled by the internal particle diffusion model and it was found that the adsorption process could be divided into three stages. In the external surface adsorption process, the rate depended on the specific surface area. During the particle diffusion stage, pore structure and volume were the main factors affecting adsorption rate. In the final equilibrium stage, the rate was determined by the ratio of meso-and macro-pores to total pore volume. The rate over the whole adsorption process was dominated by the toluene concentration. The desorption behavior of toluene on activated carbons was investigated,and the process was divided into heat and mass transfer parts corresponding to emission and diffusion mechanisms, respectively. Physical adsorption played the main role during the adsorption process.

关键词： Activated carbon Toluene Adsorption Desorption Kinetics

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从三焦理论辨治痛风

辽宁中医药大学学报 2024年第3期26卷 75-79页

作者：刘佳马宝东莫成荣李中宇辽宁中医药大学附属第二医院辽宁沈阳110034 辽宁中医药大学附属医院辽宁沈阳110032

痛风主要临床表现为高尿酸血症、急慢性关节炎、痛风石、痛风性肾病等,严重者可发生关节致残、肾功能不全,其病程长,易反复,且发病率呈上升趋势。中医药治疗痛风在改善患者临床症状、减轻药物不良反应、延缓疾病进展等方面取得了较好的... 详细信息

痛风主要临床表现为高尿酸血症、急慢性关节炎、痛风石、痛风性肾病等,严重者可发生关节致残、肾功能不全,其病程长,易反复,且发病率呈上升趋势。中医药治疗痛风在改善患者临床症状、减轻药物不良反应、延缓疾病进展等方面取得了较好的成绩。该文基于痛风的生理病理以及三焦功能特点,总结痛风发病的主要病机是三焦功能失司,脏腑功能失调,水液代谢失常,湿、热、浊毒、痰、瘀等内流五脏六腑,外阻关节经络,从而引起痛风的发生。在“三焦辨证”理论指导下,三焦并治,从整体出发,根据疾病的主证和兼证,结合患者临床病情变化,辨别上、中、下三焦的主次,以辨证论治来调整脏腑,通利三焦,以此指导临床不同阶段的治疗,为临床痛风的诊治提供一种新的辨证治疗思路。

关键词：三焦理论痛风辨证论治

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续骨伸筋汤治疗儿童尺桡骨远端骨折疗效观察

儿科药学杂志 2024年第8期30卷 40-44页

作者：计忠宇于鸿尹同珍马红如周龙龙张青河北省沧州中西医结合医院河北沧州061001

目的:探讨续骨伸筋汤联合手法复位小夹板固定治疗儿童尺桡骨远端骨折的临床疗效。方法:采用随机数表法,将70例尺桡骨远端干骺端骨折患儿随机分为对照组和干预组各35例,对照组采用手法复位小夹板固定治疗,用4块小夹板固定。干预组在对照... 详细信息

目的:探讨续骨伸筋汤联合手法复位小夹板固定治疗儿童尺桡骨远端骨折的临床疗效。方法:采用随机数表法,将70例尺桡骨远端干骺端骨折患儿随机分为对照组和干预组各35例,对照组采用手法复位小夹板固定治疗,用4块小夹板固定。干预组在对照组治疗基础上联合续骨伸筋汤治疗,先以蒸汽熏蒸,待药液温度适宜时,再用毛巾浸药液敷于患部,每次30 min,每日2次,每2周为1个疗程。从康复疗效指标、腕关节活动度等方面比较两种疗法的差异。结果:(1)对照组、干预组的总有效率分别为80.0%、94.3%,差异无统计学意义(P>0.05)。但干预组疼痛缓解时间、肿胀缓解时间及骨折愈合时间均短于对照组(P<0.01)。(2)治疗后,干预组患儿的疼痛、关节肿胀、肢体萎缩、瘀斑等症状评分均低于对照组(P<0.05);功能活动、自主活动、腕关节功能评分均高于对照组(P<0.05)。(3)拆除小夹板后,两组患儿的腕关节活动度指标均有所提高,但与对照组比较,干预组患儿的腕关节活动度指标,包括背伸度、旋前度、桡偏度、掌屈度、旋后度等均更好(P<0.05)。结论:续骨伸筋汤联合手法复位小夹板固定治疗儿童尺桡骨远端骨折的疗效确切,值得临床推广应用。

关键词：续骨伸筋汤尺桡骨远端骨折手法复位小夹板固定

Injectable hydrogels for spinal cord injury repair

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Engineered Regeneration 2022年第4期3卷 407-419页

作者： Huan Wang Hui Zhang zhongyu Xie Keng Chen Mengjun ma Yuejiao Huang Minli Li Zhaopeng Cai Peng Wang Huiyong Shen The Eighth Affiliated Hospital Sun Yat-Sen UniversityShenzhen 518033China School of Life Science and Technology Southeast UniversityNanjing 210096China State Key Laboratory of Bioelectronics School of Biological Science and Medical EngineeringSoutheast UniversityNanjing 210096China

Spinal cord injury(SCI)often causes severe functional impairment of body,which leads to a huge burden to the patient and the whole *** strategies,especially biomaterials,have been employed for SCI *** various biomaterials,injectable hydrogels have attracted much attention because of their ability to load functional components and be injected into the lesioned area without *** this review,we summarize the recent progress in injectable hydrogels for SCI *** firstly introduce the pathophysiology of SCI,which reveals the mechanism of clinical manifestations and determines the therapeutic ***,we describe the original sources of polymers and the crosslinking manners in forming *** that,we focus on the in vivo therapeutic strategies and effects of injectable ***,the recent challenges and future outlook of injectable hydrogel for SCI repair are concluded and *** believe this review can be helpful and inspire the further development of injectable hydrogels for SCI repair.

关键词： Injectable hydrogel Spinal cord injury Extracellular matrix Neuroprotection Nerve regeneration