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番茄糖基转移酶基因SlUDP提高拟南芥镉胁迫耐性的作用研究

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植物遗传资源学报 2024年第8期25卷 1396-1408页

作者：张美玉马玉芳罗宇芳耿鑫鑫郭静张欣欣陈超金晓霞哈尔滨师范大学生命科学与技术学院/黑龙江省道地野生药材种质资源研究中心

镉（Cd）胁迫严重限制植物生长，因此鉴定与植物镉胁迫耐受性相关的基因尤为重要。课题组前期通过转录组数据筛选获得番茄UDP-糖基转移酶基因（SlUDP）响应植株镉胁迫反应。本研究克隆SlUDP基因编码区全长序列，该基因在叶片和果实中表... 详细信息

镉（Cd）胁迫严重限制植物生长，因此鉴定与植物镉胁迫耐受性相关的基因尤为重要。课题组前期通过转录组数据筛选获得番茄UDP-糖基转移酶基因（SlUDP）响应植株镉胁迫反应。本研究克隆SlUDP基因编码区全长序列，该基因在叶片和果实中表达量较高，受镉胁迫诱导上调表达。酵母耐镉性分析表明，转入SlUDP基因提高了酵母镉胁迫的耐受性。进一步获得SlUDP过表达拟南芥株系，CdCl2胁迫下（40、60、80μmol/L），与野生型相比，过表达拟南芥株系的子叶失绿程度下降；发芽率、根长和种子存活率提高，而丙二醛含量下降，可溶性糖含量、超氧化物歧化酶活性、过氧化物酶活性增加，且金属离子转运蛋白基因ZIP1、IRT1、CSD1和COPT2的表达水平显著高于野生型。结果表明，SlUDP过表达株系通过调节抗氧化酶系统，提高植株清除活性氧能力，降低膜脂过氧化程度，提高金属离子转运等方面提高植株耐镉性。本研究为糖基转移酶基因在植物耐受镉胁迫中的作用研究提供一定的理论依据，并为园艺植物抗性分子育种提供了候选基因。

关键词：番茄镉糖基转移酶基因抗氧化酶系统金属转运基因

Promoting effect of polyoxyethylene octylphenol ether on Cu/ZnO catalysts for low-temperature methanol synthesis

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Journal of Natural Gas Chemistry 2009年第3期18卷 375-378页

作者： Ling Liu Tiansheng Zhao Qingxiang ma yufang Shen Key Laboratory of Energy Resources ＆ Chemical Engineering of Ningxia Ministry-Province Co-Cultivated State Key Laboratory Base Ningxia University Yinchuan 750021 Ningxia China

Cu/ZnO catalysts were prepared by the co-precipitation method with the addition of OP-10 （polyoxyethylene octylphenol ether） and were chemically and structurally characterized by means of XRD, BET, H2-TPR, CO-TPD and N20-titration. The effect of OP-10 addition on the activity of Cu/ZnO for the slurry phase methanol synthesis at 150℃ was evaluated. The results showed that Cu/ZnO prepared with addition of 8% OP-10 （denoted as C8） exhibited the promoted activity for the methanol synthesis. The conversion of CO and the STY （space time yield） of methanol were 42.5% and 74.6% higher than those of Cu/ZnO prepared without addition of OP-10 （denoted as CO）, respectively. The precursor of C8 contained more aurichalcite and rosasite, and the concerted effect of Cu-Zn in C8 was found to be stronger than that in CO. Compared with CO, C8 showed smaller particle size, lower reduction temperature and larger BET and Cu surface areas.

关键词： surfactant Cu/ZnO low-temperature methanol synthesis

基于SNP芯片的固原鸡遗传结构与保种效果分析

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浙江农业学报 2024年第7期36卷 1530-1536页

作者：张海洋黄宣周薇向鑫马玉芳尹兆正浙江大学动物科学学院浙江杭州310058 宁夏好水川食品有限公司宁夏固原756099

了解固原鸡的保种效果,为更好地保护和利用固原鸡这一重要地方种质资源提供参考和帮助。从固原鸡群体中随机采集公鸡、母鸡各30只血样,采用“京芯一号”55K芯片技术对固原鸡进行保种分析。结果显示,固原鸡群体有效含量为8.9,多态标记比... 详细信息

了解固原鸡的保种效果,为更好地保护和利用固原鸡这一重要地方种质资源提供参考和帮助。从固原鸡群体中随机采集公鸡、母鸡各30只血样,采用“京芯一号”55K芯片技术对固原鸡进行保种分析。结果显示,固原鸡群体有效含量为8.9,多态标记比例为0.828,期望杂合度为0.360,观测杂合度为0.359,说明该群体遗传变异较小,整齐度较高;G矩阵表明该群体中存在近亲交配的趋势;在检测到的1431个长纯合片段(runs of homozygosity,ROH)中,ROH长度在50~100 Mb的个体数量最多,占比达36.67%,该群体平均近交系数为0.11。结合基因组亲缘关系分析和聚类分析结果,现有30只公鸡样本可以划分为10个家系,其中25只母鸡分别划入不同家系,另外5只母鸡和所检测的公鸡亲缘关系系数均小于0.1,亲缘关系都比较远,因此,将它们归入了“其他”分类中。综上所述,该保种固原鸡群体遗传变异较小,选育程度较高,为降低群体的近交增量,后期的保种过程中需考虑亲缘关系再进行配种。

关键词：固原鸡保种分析 SNP芯片亲缘关系

口服太子参皂苷对鸡免疫功能和炎症反应的影响

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江西农业大学学报 2024年第4期46卷 980-990页

作者：廖丽华郑娱许高琳陈沛予马玉芳王全溪福建农林大学动物科学学院/中西兽医结合与动物保健福建省高等学校重点实验室/福建省兽医中药与动物保健重点实验室福建福州350002

【目的】旨在研究口服太子参皂苷(RP)对鸡免疫功能和炎症反应的影响,为使用RP作为免疫增强剂提供科学依据,对家禽养殖业的疾病预防和健康维护具有实际应用价值。【方法】试验以鸡毒支原体(MG)抗原、抗体均为阴性的30日龄健康河田鸡为研... 详细信息

【目的】旨在研究口服太子参皂苷(RP)对鸡免疫功能和炎症反应的影响,为使用RP作为免疫增强剂提供科学依据,对家禽养殖业的疾病预防和健康维护具有实际应用价值。【方法】试验以鸡毒支原体(MG)抗原、抗体均为阴性的30日龄健康河田鸡为研究对象,根据初始体质量平均分为5组,空白组不给药及疫苗,疫苗对照组口服灭菌生理盐水7 d后免疫MG弱毒疫苗,100,20,5 mg/kg RP试验组分别连续口服RP 7 d后免疫MG弱毒疫苗,一免后28 d进行二免,试验期49 d。采用间接ELISA法检测血清中MG特异性抗体消长规律及抗体阳性率和细胞因子(IFN-γ、IL-4、IL-5、IL-1α、CCL4、IL-17A、IL-6)含量。【结果】(1)RP组与疫苗对照组相比,口服RP能有效提高一免及二免后7 d的MG特异性抗体滴度及抗体阳性率(P<0.05),其中以RP高剂量(100 mg/kg)效果最佳。(2)细胞因子表达水平中,与疫苗对照组相比,RP高剂量(100 mg/kg)和中剂量(20 mg/kg)组血清中免疫因子(IFN-γ、IL-4、IL-5)的含量在一免后3 d呈现显著上升(P<0.05),一免后7 d无明显差异;(3)与疫苗对照组相比,RP组炎症细胞因子(IL-1α、CCL4、IL-17A、IL-6)血清中的含量显著降低(P<0.05),高剂量组(100 mg/kg)下降最为显著。【结论】首免前口服太子参皂苷(RP)能够提高河田鸡的免疫功能,并降低由MG弱毒疫苗引起的炎症反应,其中100 mg/kg RP效果最佳。

关键词：太子参皂苷鸡毒支原体弱毒疫苗免疫功能细胞因子

A review of gas-phase chemical mechanisms commonly used in atmospheric chemistry modelling

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Journal of Environmental Sciences 2023年第1期123卷 522-534页

作者： Yanhui Liu Jiayin Li yufang ma Ming Zhou Zhaofeng Tan Limin Zeng Keding Lu Yuanhang Zhang State Key Joint Laboratory of Environmental Simulation and Pollution Control College of Environmental Science and EngineeringPeking UniversityBeijing 100871China Institute of Energy and Climate Research IEK-8:TroposphereForschungszentrum Jülich GmbHJülich 52428Germany

The atmospheric chemical mechanism is an essential component of airshed models used for investigating the chemical behaviors and impacts of *** the first tropospheric chemical mechanism was proposed in the 1960s,various mechanisms including master Chemical Mechanism(MCM),Carbon Bond Mechanism(CBM),Statewide Air Pollution Research Center(SAPRC)and Regional Atmospheric Chemistry Mechanism(RACM)have been developed for different research *** work summarizes the development and applications of these mechanisms,introduces their compositions and lumping methods,and compares the ways the mechanisms treat radicals with box model *** can reproduce urban pollution events with relatively low cost compared to SAPRC and RACM,whereas the chemical behaviors of radicals and the photochemical production of ozone are described in detail in *** photolysis rates of some oxygenated compounds are low in SAPRC07,which may result in underestimation of radical *** an explicit chemical mechanism,MCM describes the chemical processes of primary pollutants and their oxidation products in *** can be used to investigate certain chemical processes;however,due to its large size,it is rarely used in regional model simulations.A box model case study showed that the chemical behavior of OH and HO_(2)radicals and the production of ozone were well described by all *** and SAPRC underestimated the radical levels for different chemical treatments,leading to low ozone production values in both *** and RACM are widely used in box model studies,while CBM and SAPRC are often selected in regional simulations.

关键词： Atmospheric chemistry Gas-phase chemical mechanism Ozone Box model

玉屏风煎剂对德化黑鸡生长性能、免疫调节、血清生化指标的影响

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福建农业学报 2024年第4期39卷 369-377页

作者：林雪玲胡美华林祚贵徐磊刘志军马玉芳福建农业职业技术学院福建福州350007 德化县戴云黑鸡养殖有限公司福建德化362500 中西兽医结合与动物保健福建省高等学校重点实验室福建福州350002

【目的】探讨玉屏风煎剂对德化黑鸡生长性能、免疫调节、血清生化指标的影响,并对其安全性进行观察。【方法】黄芪、白术、防风按质量比2:2:1混合煎制,浓缩成含生药1 g·mL^(-1)的玉屏风煎剂。将30日龄体重相近的德化黑鸡160头随机分为... 详细信息

【目的】探讨玉屏风煎剂对德化黑鸡生长性能、免疫调节、血清生化指标的影响,并对其安全性进行观察。【方法】黄芪、白术、防风按质量比2:2:1混合煎制,浓缩成含生药1 g·mL^(-1)的玉屏风煎剂。将30日龄体重相近的德化黑鸡160头随机分为低剂量组(1.25 mL·头^(-1)·天^(-1))、中剂量组(1.875 mL·头^(-1)·天^(-1))、高剂量组(2.5mL·头^(-1)·天^(-1))和空白对照组(0 mL·头^(-1)·天^(-1)),每组4个重复,每个重复10头,煎制玉屏风饮水给药,饲喂试验为期25 d;试验第25 d采血及十二指肠,检测生长性能、十二指肠形态结构、免疫功能、抗氧化能力、血清生化等相关指标。【结果】与对照组相比,1)中、高剂量组日增重分别提高16.77%(P0.05)。【结论】玉屏风煎剂可以提高德化黑鸡的生长性能,改善肠道形态结构,强化抗氧化能力,增强免疫功能,具有抗炎、维持免疫平稳态的作用,使用安全,最适剂量为2.5 mL·头^(-1)·天^(-1)。

关键词：玉屏风煎剂德化黑鸡生长性能肠道形态免疫功能抗氧化功能安全性

玉屏风加减煎剂对德化黑鸡柔嫩艾美耳球虫的抗感染效果

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福建农业学报 2024年第6期39卷 633-642页

作者：林雪玲林祚贵赖宝色徐磊刘志军马玉芳福建农业职业技术学院福建福州350007 德化县戴云黑鸡养殖有限公司福建德化362500 中西兽医结合与动物保健福建省高等学校重点实验室(福建农林大学) 福建福州350002

【目的】探明玉屏风加减煎剂对柔嫩艾美耳球虫(Eimeria tenella)感染德化黑鸡抗氧化、抗炎、免疫功能的影响及抗球虫效果。【方法】将120只45日龄德化黑鸡随机分为6组,分别是空白对照组(K组)、感染对照组(G组)、常山青蒿低剂量组(CQL组... 详细信息

【目的】探明玉屏风加减煎剂对柔嫩艾美耳球虫(Eimeria tenella)感染德化黑鸡抗氧化、抗炎、免疫功能的影响及抗球虫效果。【方法】将120只45日龄德化黑鸡随机分为6组,分别是空白对照组(K组)、感染对照组(G组)、常山青蒿低剂量组(CQL组)、玉屏风加减低剂量组(YL组)、常山青蒿高剂量组(CQH组)、玉屏风加减高剂量组(YH组),除空白对照组外,其余各组均感染柔嫩艾美耳球虫,24 h后开始连续7 d饮水给药,第8 d测定比较各组抗球虫指数、免疫功能、抗氧化能力等指标的差异。【结果】与感染对照组(G组)相比,中药处理组CQH、YH、CQL、YL的血便、盲肠病变症状依次减轻。除CQH组外,其他组盲肠病变计分、盲肠黏膜下淋巴细胞密度显著或极显著下降(P<0.05或P<0.01);除CQH组OPG显著下降(P<0.05)外,其他3个中药组OPG下降极显著(P<0.01);YL组和YH组盲肠上皮细胞脱落计分极显著降低(P<0.01)。与CQL、CQH组相比,YL、YH组MDA、盲肠病变计分、盲肠上皮细胞脱落计分和OPG下降极显著(P<0.01)。与G组相比,4个中药组IL-6、 IFN-γ、TNF-α、丙二醛(MDA)含量极显著降低(P<0.01),而IL-2含量、 SIgA水平、谷胱甘肽过氧化物酶(GSH-Px)活性、超氧化物歧化酶(SOD)活性、过氧化氢酶(CAT)活性、总抗氧化能力(T-AOC)极显著升高(P<0.01);YL组IL-2含量、 SIgA水平、GSH-Px活性、SOD活性、CAT活性极显著增高(P<0.01),YH组GSH-Px、 SOD活性极显著升高(P<0.01)。与K组相比,G组、CQL组、YL组、CQH组、YH组的相对增重率分别是79.88%、 103.49%、 107.27%、 95.05%、 96.06%,抗球虫指数(anti-coccidialindex,ACI)分别是96、165.5、177、133.5、162.5。【结论】玉屏风加减煎剂对人工感染柔嫩艾美耳球虫德化黑鸡具有更好抗炎、抗氧化、提高免疫功能的免疫调节作用,并相应具有更好的抗球虫效果,且以玉屏风加减低剂量组(YL)抗球虫效果最好。

关键词：玉屏风加减煎剂德化黑鸡柔嫩艾美耳球虫免疫调节抗球虫指数

Inhibitory effects of cytosine deaminase gene-transfected bone marrow mesenchymal stem cells on glioma cell proliferation

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Neural Regeneration Research 2011年第16期6卷 1238-1242页

作者： Fei Song Qi Xing Kedong Song Jian Liu Guangchun Ji yufang ma Tianqing Liu Minghai Wei Xuehu ma Dalian R&D Center for Stem Cells and Tissue Engineering Dalian University of Technology Dalian 116024 Liaoning Province China Department of Neurosurgery Second Hospital Affiliated to Dalian Medical University Dalian 116027 Liaoning Province China Department of Anesthesiology First Hospital Affiliated to Dalian Medical University Dalian 116011 Liaoning Province China Department of Biochemistry and Molecular Biology Dalian Medical University Dalian 116044 Liaoning Province China

Bone marrow mesenchymal stem cells were isolated from C57BL mice, transfected with the cytosine deaminase （CD） gene using a lentivirus vector and co-cultured with C6 glioma cells to verify anti-tumor effects of bone marrow mesenchymal stem cells carrying CD genes. C57MSC-CD/eGFP cells converted 5-fluorocytosine to 5-fluorouracil and exhibited significant inhibition of proliferation and apoptosis in C6 glioma cells. C57MSC-CD/eGFP cells were then implanted into rat models of brain C6 glioma. Rats were also intraperitoneally injected with 5-fluorocytosine after 7 days. MSC-CD/eGFP cells were irregularly distributed at the margin of the glioma, as well as encased and reduced the volume of the glioma. CD-transfected bone marrow mesenchymal stem cells inhibit the in vivo growth and in vitro proliferation of glioma.

关键词： magnetic resonance imaging glioma gene therapy cytosine deaminase gene bone marrow mesenchymal stem cells lentivirus

Comparative study of the promotion of porcine fetal fibroblast proliferation by overexpression of two transcriptional variants of SIRT6

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Chinese Science Bulletin 2013年第10期58卷 1169-1174页

作者： XIE XiaoXian HE Kan ZHANG AiLing ZHANG Hao WANG QiShan LI JiaQi ma yufang School of Agriculture and Biology Department of Animal SciencesShanghai Jiao Tong UniversityShanghai Key Laboratory of Veterinary Biotechnology Department of Animal Genetics Breeding and ReproductionCollege of Animal SciencesSouth China Agricultural University Guangdong Provincial Key Laboratory of Agroanimal Genomics and Molecular Breeding College of Animal SciencesSouth China Agricultural University

mammalian Sirtuin 6(SIRT6) plays an important role in the transcriptional silencing of silent mating-type loci,telomeres,and ***,the roles of porcine SIRT6 in cell proliferation are poorly understood,and a better knowledge of these will help improve our understanding of the biological mechanisms of cell growth and *** this study,a novel variant of porcine SIRT6(SIRT6 V2) identified by reverse transcription-polymerase chain reaction and BLAST analysis showed a 124-bp deletion compared to wild-type SIRT6 mRNA(SIRT6 V1).Two recombinant plasmids overexpressing SIRT6 V1 and SIRT6 V2 were produced and their roles in the proliferation of porcine fetal fibroblasts(PFFs) were *** transfected with SIRT6 V1 proliferated significantly faster than those transfected with SIRT6 V2(P<0.01),though both proliferated significantly faster than cells expressing an empty vector(P<0.01).These results might be caused by altered proportions of α helices and β sheets in the SIRT6 V2 structure relative to SIRT6 *** results indicate that overexpression of SIRT6 V1/V2 was involved in promoting PFF *** of a 124-bp sequence attenuated the effects of SIRT6 on cell proliferation,possibly as a result of changes in the proportions of α helices and β sheets in the protein secondary structure.

关键词：成纤维细胞增殖胎儿成纤维细胞 DNA转录过度表达猪变体聚合酶链反应 BLAST