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Genome-wide CRISPR screening reveals key genes and pathways associated with 20-hydroxyecdysone signal transduction in the silkworm(Bombyx mori)

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Insect Science 2024年第1期31卷 47-58页

作者： Hao Sun Jingya Chen Ruolin Wang Dan Liu Na Zhang Tong Zhang Ling Jia Sanyuan ma Qingyou xia State Key Laboratory of Silkworm Genome Biology Southwest UniversityChongqingChina Biological Science Research Center Southwest UniversityChongqingChina

Metamorphosis is a complex developmental process involving multiple pathways and a large number of genes that are regulated by juvenile hormone(JH)and 20-hydroxyecdysone(20E).Despite important progress in understanding various aspects of silkworm biology,the hormone signaling pathway in the silkworm remains poorly ***-wide screening using clustered regularly interspaced short palindromic repeats(CRISPR)/CRISPR-associated protein 9(Cas9)-based libraries has recently emerged as a novel method for analyzing genome function,enabling further research into essential genes,drug targets,and virus-host ***,we constructed a genome-wide CRISPR/Cas9-based library of the silkworm(Bombyx mori)and successfully revealed the genes involved in biotic or abiotic stress factor *** this study,we used our silkworm CRISPR library and large-scale genome-wide screening to analyze the key genes in the silkworm 20E signaling pathway and their mechanisms of *** annotation showed that 20E regulates key proteins in processes that mainly occur in the cytoplasm and *** enrichment analysis showed that 20E can activate phosphorylation and may affect innate immunity,interfere with intracellular nutrition and energy metabolism,and eventually cause cell *** screening results were experimentally validated by generating cells with knockout alleles of the relevant genes,which had increased tolerance to *** findings provide a panoramic overview of signaling in response to 20E in the silkworm,underscoring the utility of genome-wide CRISPR mutant libraries in deciphering hormone signaling pathways and the mechanisms that regulate metamorphosis in insects.

关键词： CRISPR/Cas9 CRISPR library screening ecdysone 20E signal transduction silkworm whole genome editing

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Investigating Sulfur Chemistry in the HD163296 Disk

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Research in Astronomy and Astrophysics 2024年第7期24卷 215-225页

作者： Rong ma Donghui Quan Yan Zhou Jarken Esimbek Dalei Li xiaohu Li xia Zhang Juan Tuo Yanan Feng Xinjiang Astronomical Observatory Chinese Academy of SciencesUrumqi 830011China University of Chinese Academy of Sciences Beijing 100049China Research Center for Intelligent Computing Platforms Zhejiang LaboratoryHangzhou 311100China BinZhou University Binzhou 256600China Key Laboratory of Radio Astronomy Chinese Academy of SciencesUrumqi 830011China Xinjiang Key Laboratory of Radio Astrophysics Urumqi 830011China

Sulfur chemistry in the formation process of low-mass stars and planets remains poorly *** protoplanetary disks are the birthplace of planets and its distinctive environment provides an intriguing platform for investigating models of sulfur *** analyzed the Atacama Large Millimeter/submillimeter Array observations of CS 7-6 transitions in the HD 163296 disk and performed astrochemical modeling to explore its sulfur *** simulated the distribution of sulfur-containing molecules and compared it with observationally deduced fractional column *** have found that the simulated column density of CS is consistent with the observationally deduced fractional column densities,while the simulated column density of C_(2)S is lower than the observationally deduced upper limits on column *** results indicate that we have a good understanding of the chemical properties of CS and C_(2)S in the *** also investigated the influence of the C/O ratio on sulfur-containing molecules and found that the column densities of SO,SO_(2),and H_2S near the centra star are dependent on the C/O ***,we found that the N[CS]/N[SO]ratio can serve as a promising indicator of the disk’s C/O ratio in HD ***,the disk of HD 163296 provides a favorable environmen for the detection of sulfur-containing molecules.

关键词： Protoplanetary Disks-Astrochemistry-Molecular Processes

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数值模拟在陶瓷粉末压制成型中的应用

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人工晶体学报 2007年第2期36卷 415-418,423页

作者：王次明杨正方马红霞天津大学材料学院先进陶瓷与加工技术教育部重点实验室天津300072 中材人工晶体研究院北京100018

本文介绍了陶瓷粉末成型过程有限元数值模拟所需用的Drucker-Prager-Cap模型以及材料屈服准则,分别概述了数值模拟方法在陶瓷粉末干压法成型、冷等静压法成型中的应用进展及存在的问题,并介绍了有限元分析在成型件开裂方面的研究进展。

关键词：数值模拟陶瓷粉末干压成型冷等静压

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Ultra-homogeneous dense Ag nano layer enables long lifespan solid-state lithium metal batteries

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Journal of Energy Chemistry 2024年第9期96卷 110-119页

作者： Yaning Liu Tianqi Yang Ruyi Fang Chengwei Lu Ruojian ma Ke Yue Zhen xiao xiaozheng Zhou Wenkui Zhang Xinping He Yongping Gan Jun Zhang Xinhui xia Hui Huang Xinyong Tao Yang xia College of materials Science and Engineering Zhejiang University of TechnologyHangzhou 310014ZhejiangChina Key Laboratory of Rare Earth Optoelectronic materials and Devices of Zhejiang Province Institute of Optoelectronic Materials and DevicesChina Jiliang UniversityHangzhou 310018ZhejiangChina

The unstable electrolyte/lithium(Li)anode interface has been one of the key challenges in realizing high energy density solid-state lithium metal batteries(LMBs)***,a dense and uniform silver(Ag)nano interlayer with a thickness of∼35 nm is designed accurately by magnetron sputtering technology to optimize the electrolyte/Li anode *** Ag nano layer reacts with Li metal anode to in-situ form Li-Ag alloy,thus enhancing the physical interfacial contact,and further improving the interfacial wettability and *** particular,the Li-Ag alloy is inclined to form AgLi phase proved by cryo-TEM and DFT,effectively preventing SN from continuously“attacking”the Li metal anode due to the lower adsorption of succinonitrile(SN)molecules on AgLi than that of pure Li metal,thereby significantly reinforcing the interfacial ***,the enhanced physical and chemical stability of electrolyte/Li anode interface promotes the homogeneous deposition of Li^(+)and inhibits the dendrite *** Li-symmetric cell maintains stable operation for up to 1700 h and the cycling stability of LiFePO_(4)|SPE|Li full cell is remarkably improved at room temperature(capacity retention rate of 91.9%for 200 cycles).This work opens an effective way for accurate and controllable interface design of long lifespan solid-state LMBs.

关键词： Silvernano layer Poly(ethylene oxide) Solid polymer electrolyte Succinonitrile Lithium metal battery

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Sequence-Controlled Synthesis of Alternating Poly(ester amide)s using Water as Control Agent in One-Pot

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Chinese Journal of Polymer Science 2024年第8期42卷 1015-1020页

作者： Tao Liu Fang-Fang Niu Kan Cheng Yu-Min xia Bo-Mou ma Xue-Li Wang Jian-Yong Yu Yong He State Key Laboratory for Modification of Chemical Fibers and Polymer materials College of Materials Science and EngineeringDonghua UniversityShanghai 201620China Innovation Center for Textile Science and Technology Donghua UniversityShanghai 201620China

Poly(ester amide)s(PEAs)attract much attention as a new kind of biodegradable ***,the synthesis of PEAs with sequence-regulated chain structures is still complicated due to the multistep polymerization,high monomer purity,as well as the usage of organic solvents,which greatly inhibits its development and ***,a one-pot strategy without solvent was developed to synthesize the alternating PEA fromα,ω-amino alcohol and dicarboxylic acid by sequential polycondensation,where water was used as the controlling agent for amidation and ***,the amidation and esterification were controlled to proceed in turn by adding or removing water,realizing the one-pot preparation of alternating *** resultant PEA is characterized by^1H-and^(13)C-NMR,and its chain structure is confirmed to be highly quasi-alternating similar to alternating PEAs prepared by classical methods The evaluations from DSC and Dma demonstrate that the properties of quasi-alternating PEA(T_(m)=102℃,ΔH_(m)=30J·g^(-1))are far superior to those of random PEA(T_(m)=72℃,ΔH_(m)<1 J·g^(-1)).

关键词： Biodegradable macromolecules Polymer architectures Sequence-controlled synthesis One-pot polymerization

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Short-term Association of Meteorological Elements on COVID-19 Pandemic in a Semi-arid City of Northwest China,2021-2022

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Biomedical and Environmental Sciences 2024年第5期37卷 537-542页

作者： FENG Feng Liu ma Yu xia CHENG Bo Wen LIU Zong Rui ZHAO Yu Han YANG Jie College of Atmospheric Sciences Key Laboratory of Semi-Arid Climate ChangeMinistry of EducationLanzhou UniversityLanzhou 730000GansuChina Minhe Meteorological Bureau Minhe 810800QinghaiChina

The coronavirus disease 2019(COVID-19)outbreak in late 2019 significantly impacted global *** virus’s rapid spread,with its long incubation period and high infection level,caused substantial disruption to regular social frameworks,production,and lifestyles^([1]).COVID-19 is primarily transmitted through close contact,long-range contaminants,and airborne routes.

关键词： Northwest COV primarily

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2005-2014年句容市法定传染病流行趋势分析

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现代预防医学 2016年第5期43卷 782-785页

作者：陈少军马红霞南京医科大学公共卫生学院句容市疾病预防控制中心

目的了解2005-2014年句容市法定传染病流行规律,为有效防控疫情提供科学依据。方法对疫情资料进行流行病学描述性分析,采用Excel2003和SPSS19.0统计分析,率的比较用检验,P<0.05为有统计学意义。结果句容市2005-2014年共报告法定传染病25... 详细信息

目的了解2005-2014年句容市法定传染病流行规律,为有效防控疫情提供科学依据。方法对疫情资料进行流行病学描述性分析,采用Excel2003和SPSS19.0统计分析,率的比较用检验,P<0.05为有统计学意义。结果句容市2005-2014年共报告法定传染病25种19657例,年均发病率321.78/10万,呈上升趋势(趋势=463.72,P<0.005);死亡38例,死亡率0.62/10万,病死率0.19%;肠道、呼吸道、自然疫源性及虫媒传染病和血源及性传播疾病的发病率(1/10万)分别为145.38、129.99、1.19和45.08。报告发病数居前五位的病种分别为手足口病、肺结核、流行性腮腺炎、病毒性肝炎、细菌性痢疾,构成比分别为32.77%(6441例)、23.06%(4533例)、11.50%(2260例)、7.69%(1511例)、7.23%(1421例)。男女性别比为1.64:1,发病率分别为418.87/10万、233.29/10万,年龄构成小年龄组高于其它年龄组;职业分布以农民、婴幼儿和学生为主;城区发病率高于农村;时间分布4-7月份和10-12月份呈现两个高峰。结论 2005-2014年句容市法定传染病总体呈上升趋势;传播途径以肠道和呼吸道传播为主;手足口病、肺结核、流行性腮腺炎、病毒性肝炎、细菌性痢疾、梅毒等为主要传染病,应调整防控策略,有效控制传染病流行。

关键词：法定传染病发病率流行趋势

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体育课同班教学对失聪大学生心理健康的影响

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体育学刊 2006年第4期13卷 80-82页

作者：马宏霞中州大学体育部河南郑州450005

采用症状自评量表(SCL-90)和自编问卷测查,发现失聪大学生的心理健康水平明显低于普通大学生。采用失聪生与普通学生同班教学的方式,对失聪大学生实施教育干预,重点以改变其自卑、孤僻、固执、多疑等心理。结果表明,失聪大学生的心理健... 详细信息

采用症状自评量表(SCL-90)和自编问卷测查,发现失聪大学生的心理健康水平明显低于普通大学生。采用失聪生与普通学生同班教学的方式,对失聪大学生实施教育干预,重点以改变其自卑、孤僻、固执、多疑等心理。结果表明,失聪大学生的心理健康水平有提高,并且朝着普通大学生的正常的心理健康方向发展。

关键词：同班教学体育课失聪生高校心理健康

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Effectiveness of Histopathological Examination of Ultrasound-guided Puncture Biopsy Samples for Diagnosis of Extrapulmonary Tuberculosis

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Biomedical and Environmental Sciences 2024年第2期37卷 170-177页

作者： GU Wen Fei SHI xia ma Xin YU Jun Lei XU Jin Chuan QIAN Cheng Cheng HU Zhi Dong ZHANG Hui Shanghai Public Health Clinical Center Fudan UniversityShanghai 201508China School of Laboratory Medicine and Life Science Wenzhou Medical UniversityWenzhou 325000ZhejiangChina Department of Laboratory Medicine Zhejiang Putuo HospitalZhoushan 316100ZhejiangChina Zhongshan Hospital Fudan UniversityShanghai 200032China

Objective To evaluate the diagnostic value of histopathological examination of ultrasound-guided puncture biopsy samples in extrapulmonary tuberculosis(EPTB).Methods This study was conducted at the Shanghai Public Health Clinical Center.A total of 115patients underwent ultrasound-guided puncture biopsy,followed by MGIT 960 culture(culture),smear,Gene Xpert MTB/RIF(Xpert),and histopathological *** assays were performed to evaluate their effectiveness in diagnosing EPTB in comparison to two different diagnostic criteria:liquid culture and composite reference standard(CRS).Results When CRS was used as the reference standard,the sensitivity and specificity of culture,smear,Xpert,and histopathological examination were(44.83%,89.29%),(51.72%,89.29%),(70.11%,96.43%),and(85.06%,82.14%),*** on liquid culture tests,the sensitivity and specificity of smear,Xpert,and pathological examination were(66.67%,72.60%),(83.33%,63.01%),and(92.86%,45.21%),*** examination showed the highest sensitivity but lowest ***,we found that the combination of Xpert and histopathological examination showed a sensitivity of 90.80%and a specificity of 89.29%.Conclusion Ultrasound-guided puncture sampling is safe and effective for the diagnosis of *** with culture,smear,and Xpert,histopathological examination showed higher sensitivity but lower *** combination of histopathology with Xpert showed the best performance characteristics.

关键词： Extrapulmonary tuberculosis Diagnosis Biopsy Histopathological examination Puncture samples

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Surface-phonon-polariton-enhanced photoinduced dipole force for nanoscale infrared imaging

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National Science Review 2024年第5期11卷 227-235页

作者： Jian Li Junghoon Jahng Xuezhi ma Jing Liang Xue Zhang Qianhao Min xiao-Liang Wang Shuangjun Chen Eun Seong Lee Xing-Hua xia State Key Lab of Analytical Chemistry for Life Science School of Chemistry and Chemical Engineering Nanjing University Hyperspectral Nano-Imaging Team Korea Research Institute of Standards and Science Institute of materials Research and Engineering Agency for ScienceTechnology and Research College of materials Science and Engineering Nanjing Tech University

The photoinduced dipole force(Pi DF) is an attractive force arising from the Coulombic interaction between the light-induced dipoles on the illuminated tip and the sample. It shows extreme sample-tip distance and refractive index dependence, which is promising for nanoscale infrared(IR) imaging of ultrathin samples. However, the existence of Pi DF in the mid-IR region has not been experimentally demonstrated due to the coexistence of photoinduced thermal force(Pi TF), typically one to two orders of magnitude higher than Pi DF. In this study, we demonstrate that, with the assistance of surface phonon polaritons, the Pi DF of c-quartz can be enhanced to surpass its Pi TF, enabling a clear observation of Pi DF spectra reflecting the properties of the real part of permittivity. Leveraging the detection of the Pi DF of phonon polaritonic substrate, we propose a strategy to enhance the sensitivity and contrast of photoinduced force responses in transmission images, facilitating the precise differentiation of the heterogeneous distribution of ultrathin samples.

关键词： surface phonon polariton photoinduced dipole force nanoscale infrared imaging ultrathin sample

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