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***-96-based therapy protect microvasculature against oxygen-induced retinopathy:a novel uncovered property of miR-96 in vascular repair

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Annals of Eye Science 2019年第1期 217-217页

作者： Michel Desjarlais Jose Carlos Rivera Isabelle Lahaie maëlle wirt Sylvain Chemtob Department of Ophthalmology Maisonneuve-Rosemont Hospital Research CenterUniversity of MontréalMontréalQCCanada Departments of Pediatrics Ophthalmology and PharmacologyCentre Hospitalier Universitaire Sainte-Justine Research CenterMontréalQCCanada

Background:Ischemic retinopathies(IRs)are ocular disorders associated to microvascular degeneration leading to visual impairments and ***(miRNAs)are a family of non-coding RNAs that regulate a wide range of gene expression involved in various biological process such blood vessel development and pathological ***,the post-transcriptional modulation of miRs and especially,their specific functions in the eyes during IRs remain to be *** aim to evaluate the potential role of miR-96 on microvascular degeneration in a rat model of oxygen-induced retinopathy(OIR).Methods:In vivo:next generation sequencing(NSG)was used to perform a complete miRNAs profiling in the retina and choroid from OIR and normoxia(CTL)*** evaluate the effects of miR-96 on microvasculature,OIR animals were treated with a miR-96 mimic(1 mg/kg)or a control-miR by intravitreal injection before hyperoxia-exposure(80%O2).Immunostaining analysis of retinal flatmounts and cryosections was used to explore the microvascular effects of *** vitro:Human Retinal Microvascular Endothelial Cells(HRMVEC)were subjected or not to hyperoxia(80%O2)and transfected with 50 nM of miR-96 mimic or *** assay was performed(tube formation and migration)and molecular analysis evaluated by qRT-PCR and western ***:NSG and qRT-PCR analyses identified miR-96 as one of most highly expressed miRNAs in retina and choroid during ***,miR-96 showed a strong downregulation in OIR rats,and also in HRMVEC subjected to *** HRMVEC,we found that miR-96 regulates positively the expression of the key pro-angiogenic factors VEGF,FGF-2 and *** better explore the role of miR-96 on HRMVEC angiogenic activity,we performed a gain/loss of function ***,to hyperoxia exposure,we observed a robust angiogenic impairment(tube formation and migration)on HMRVEC transfected with an ***,overexpression of miR-96 completely recued the

关键词： Ischemic retinopaty miRNA angiogenesis vascular repair hyperoxia

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儿童慢性乙型肝炎HBV—DNA的检测

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德国医学 1993年第1期10卷 14-15页

作者： wirt.,S 王来

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Intervention Effects of the School-Based Health Promotion Program“Join the Healthy Boat”on Motor Abilities of Children with Migration Background

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Health 2017年第3期9卷 520-533页

作者： Christine Lammle Olivia Wartha Susanne Kobel Tamara wirt Anne Kelso Claire Kutzner Jürgen M.Steinacker Division of Sports and Rehabilitation Medicine Ulm University Medical CentreUlmGermany

Purpose: Motor abilities of migrant children show worse performance compared with children without migration background. This study investigated whether a one-year intervention, which aimed to increase physical activity (PA), could influence the motor performance of these children positively. Methods: A sub-sample of 525 migrant children (7.1 ± 0.7 years;48.6% male), participated in the study and included an intervention (IG) and control group (CG). The Dordel-Koch-Test was used to assess motor performance. An exploratory factor analysis was performed. Differences between IG and CG were examined using ANCOVA. Results: Boys in the IG showed a significant improvement in the conditional skills (F(1, 201) = 8.02, p ≤ 0.005) and girls showed a tendency towards better results. Additionally, girls showed a significant improvement in flexibility (F(1, 226) = 10.72, p ≤ 0.01) compared with the CG. Conclusion: The intervention affects parts of motor skills of migrant children significantly. Future interventions should aim at a target-group-specific promotion to get a holistic improvement in their motor abilities.

关键词： Physical Activity Prevention School-Based Intervention

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