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Evaluating the efficacy of an attenuated Streptococcus equi ssp. zooepidemicus vaccine produced by multi-gene deletion in pathogenicity island SeseCisland_4

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Journal of Integrative Agriculture 2019年第5期18卷 1093-1102页

作者： ma Fang WANG Guang-yu ZHOU Hong ma zhe LIN Hui-xing FAN Hong-jie MOE Joint International Research Laboratory of Animal Health and Food Safety College of Veterinary Medicine Nanjing Agricultural University National Center of Meat Quality and Safety Control Nanjing Agricultural University Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses Yangzhou University

Streptococcus equi ssp. zooepidemicus(SEZ) is a pathogen associated with a wild range of animal species. Frequent outbreaks have occurred in recent years in pigs, horses, goats and dogs which is liable to infect humans. There is a lack of efficient vaccines against this disease and the occurrence of antibiotic resistance may render drug therapies ineffective. In this study, gene deletion mutant(ΔSEZ) in pathogenicity islands SeseCisland_4 was constructed. The mutant ΔSEZ had a 52-fold decrease in 50% lethal dose(LD_(50)) and had less capacity to adhere epithelial cells. Importantly, immunization of mice with attenuated vaccine ΔSEZ at the dose of 10~2 colony-forming units(CFU) mL^(–1) elicited a significant humoral antibody response, with an antibody titer of 1:12 800. Therefore, 10~2 CFU mL^(–1) might be used as the appropriate immune dose for the attenuated vaccine ΔSEZ, which provided mice with efficient protection against virulent SEZ. In addition, the hyperimmune sera against 10~2 CFU m L–1 attenuated vaccine ΔSEZ could confer significant protection against virulent SEZ infection in the passive immunization experiment and exhibited efficient bactericidal activity in the whole blood assay. Meanwhile, no viable bacteria was detected in blood when mice were immunized with ΔSEZ at the dose of 10~2 CFU mL^(–1) via hypodermic injection. Thereafter, the mutant ΔSEZ at the dose of 10~2 CFU mL^(–1) could confer significant protection in mice and had less negative effects on host, which could be an effective attenuated vaccine candidate for the prevention of SEZ.

关键词： Streptococcus equi ssp.zooepidemicus zoonosis attenuated vaccine immune dose

Block Copolymer Solid Electrolytes Based on Comb-Like Poly(ethylene glycol)Plasticized Poly(ionic liquid)s for Lithium-Ion Batteries

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Chinese Journal of Chemistry 2023年第19期41卷 2493-2501页

作者： Runtao Zhao Jixing Yang Bin Wang zhe ma Li Pan Yuesheng Li School of materials Science and Engineering Tianjin Key Laboratory of Composite and Functional MaterialsTianjin UniversityTianjin300072 China

Electrolytes based on poly(ionic liquid)s(PILs)have attracted great attention in the fields of next-generation solid lithium-ion ***,the low ionic conductivity prevents their practical ***,we report novel solid electrolytes based on block copolymers composed of PILs and comb-like poly(ethylene glycol)(PEG),which were synthesized via ring-opening metathesis polymerization of 3-(2-(bicyclo[2.2.1]hept-5-en-2-yl)ethyl)-1-butyl-1H-imidazol-3-ium bis((trifluoromethyl)sulfonyl)amide and poly(ethylene glycol monomethyl ether)bicyclo[2.2.1]***-like PEG acts as plasticizers in block copolymers dominated by PILs to promote the mobility of PILs *** of the copolymer composition and length of the comb-like PEG chain on ionic conductivity were *** optimized electrolyte delivers the highest ionic conductivity of 1.5×10^(–5)S·cm^(–1)at 30℃,and robust electrochemical stability up to 4.6 V.A solid-state Li/LiFePO4 cell using the optimized electrolyte demonstrates good cycle performance at 0.2 C with high capacity retention of 92%after 70 cycles at 50℃.

关键词： Solid polymer electrolytes Li-ion batteries Polymerized ionic liquids Poly(ethylene glycol) Block copolymers Electrostatic interactions

The vital role of CovS in the establishment of Streptococcus equi *** virulence

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Journal of Integrative Agriculture 2023年第2期22卷 568-584页

作者： XU Bin ma zhe ZHOU Hong LIN Hui-xing FAN Hong-jie MOE Joint International Research Laboratory of Animal Health and Food Safety College of Veterinary MedicineNanjing Agricultural UniversityNanjing 210095P.R.China Key Laboratory of Veterinary Biological Engineering and Technology of Ministry of Agriculture and Rural Affairs National Research Center of Veterinary Biologicals Engineering and TechnologyInstitute of Veterinary MedicineJiangsu Academy of Agricultural SciencesNanjing 210014P.R.China Jiangsu Co-innovation Center for the Prevention and Control of Important Animal Infectious Diseases and Zoonoses Yangzhou UniversityYangzhou 225009P.R.China

Streptococcus equi ***(SEZ)is an important zoonotic ***,a virulence-attenuated strain M35246 derived from natural variation of wild-type SEZ ATCC35246 was found.M35246 showed a deletion of 25contiguous genes as well as a loss-of-function mutation in ***,a 25-gene-deleted strain(ΔPI),a covS-mutant strain(Mcov S),and relevant complementary strains were constructed and investigated.M35246 and Mcov S were significantly less encapsulated and exhibited poorer anti-phagocytic capacity compared to wild-type *** was significantly more sensitive toβ-lactams,aminoglycosides,macrolides,and lincosamides than wild-type SEZ.M35246,McovS,andΔPI exhibited an increase in median lethal dose(LD_(50))in mice by 10~5,10~5,and 5 times when compared to wild-type SEZ,*** M35246 nor McovS were isolated from mice 48 h after being challenged with approximately 2000 times the LD_(50)of wild-type *** analysis showed that 668 significantly differentially expressed genes existed between McovS and wild-type *** virulence factor-encoding genes and anabolicrelated genes in McovS that were involved in anti-phagocytosis,capsule formation,pathogenicity,and antibiotic resistance were downregulated significantly relative to the wild-type *** study revealed that the CovS plays a vital role in the establishment of SEZ virulence.

关键词： Streptococcus equi subsp.zooepidemicus covS natural variation virulence regulation

Partitioning behavior and lattice misfit of γ/γ’phases in Ni-based superalloys with different Mo additions

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Rare Metals 2021年第4期40卷 920-927页

作者： zhe ma Yan-Ling Pei Liang Luo Lu Qin Shu-Suo Li Sheng-Kai Gong School of materials Science and Engineering Beihang UniversityBeijing 100191China

A low-density single crystal(LDS) alloy with the composition of high Mo content was *** extra 1.5 wt% Mo was added in the Alloy A with the composition of Ni-6.5 Al-8.0 Mo-2.4 Cr-6.2 Ta-4.9 Co-1.5 Re-(0.01-0.05) Y(wt%) to study the influence of Mo on the lattice parameter and partitioning *** electron microscope(SEM) with energy-dispersive spectrometer(EDS),transmission electron microscopy(TEM)and high-temperature X-ray diffraction(HT-XRD) were used to observe the microstructure,analyze the elemental content and measure the lattice parameter of the *** natural lattice misfit was calculated by lattice constants which were measured by HT-XRD at the temperature from 25 to 1150℃,and the results showed that the lattice misfit would be more and more negative with temperature *** was found that 1.5 wt% addition of Mo will increase the absolute value of the lattice misfit of γ/γ’phases and the volume fraction of γ’,and at the same time,influence the elemental distribution in γ and γ’ phases,especially Re and *** has a higher partitioning ratio(k) after the addition of Mo.

关键词： Ni-based superalloys Mo Lattice misfit High-temperature X-ray diffraction(HT-XRD) Partitioning behavior

Doublet luminescence due to coexistence of excitons and electron-hole plasmas in optically excited CH_(3)NH_(3)PbBr_(3)single crystal

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Chinese Physics B 2022年第4期31卷 597-602页

作者： Jie Wang Guang-zhe ma Lu Cao Min Gao Dong Shi School of Optoelectronic Science and Engineering University of Electronic Science and Technology of ChinaChengdu 610054China School of materials and Energy University of Electronic Science and Technology of ChinaChengdu 610054China

Doublet luminescence from hybrid metal trihalide perovskite semiconductors is observed along with materials processing when high-quality single crystals are ***,the underlying physical mechanism remains poorly ***,we report controllable solution-processed crystallization that affords high-quality CH3 NH3 PbBr3 single crystals with atomically flat pristine ***-face photoluminescence(PL)shows doublet luminescence components with variable relative intensities depending on the crystal surface *** further find that the low-energy PL component with asymmetric spectral line-shape becomes predominant when the atomically flat crystal surfaces are passivated in the ion-abundant saturated solutions,while poor-quality single crystal with visually rough surface only gives the high-energy PL with symmetric *** asymmetric spectral line-shape of the low-energy PL matches perfectly with the simulated bandedge ***,the low-energy PL component is attributable to the intrinsic bandedge emission from the crystal bulk while the high-energy one to surface-specific *** fitting to the absorption data and multi-exponential fitting to the time-resolved photoluminescence traces jointly indicate the coexistence of excitons and electron-hole plasmas in optically excited CH_(3)NH_(3)PbBr_(3)single crystals,thereby catching the physical merit that leads to the occurrence of doublet luminescence.

关键词： semiconductor crystal surface luminescence

The Influence of Various Cationic Group on Polynorbornene Based Anion Exchange Membranes with Hydrophobic Large Steric Hindrance Arylene Substituent

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Chinese Journal of Polymer Science 2023年第2期41卷 278-287页

作者： Wei Wang Da-Fu Cao Xiao-Wei Sun Li Pan zhe ma Yue-Sheng Li Tianjin Key Laboratory of Composite and Functional materials School of Materials Science and EngineeringTianjin UniversityTianjin300350China

A series of brominated polynorbornene derivatives,including bulky steric hydrophobic groups and highly physical and chemical stable backbones,were synthesized via ring-opening metathesis polymerization and post-functionalized with trimethylammonium(QA),N-methylimidazole(Im),N-methylpyrrolidinium(Pyr)or N-methylpiperidinium(Pip)to construct the entire anion exchange membranes(AEMs).Benefited from prominent phase-separated morphology,PBO-x%-y(x=66,68,70;y=QA,Im,Pyr,Pip)AEMs with ion exchange capacity(IEC)approaching 2.0 meq·g^(−1)exhibited super high hydroxide ***,PBO-70%-Pip possessed the highest hydroxide conductivity of 137.3 mS·cm^(−1)at 80℃Moreover,all membranes exhibit low swelling ratio(SR)(the SR of PBO-66%-QA was just 8.6%at 80℃).That is,bulky steric hydrophobic groups play a crucial role in balancing the high hydroxide conductivity and low SR in ***,three AEMs(PBO-66%-QA,PBO-68%-Pyr,PBO-70%-Pip)showed good alkaline stability after immersion into 1.0 mol/L NaOH aqueous solution at 80℃for 480 h without any degradation.

关键词： Anion exchange membrane Steric hindrance Phase separation Ion conductivity Swelling ratio

Influences of Graupel Microphysics on Cma-GFS Simulation of Summer Regional Precipitation

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Journal of Meteorological Research 2024年第1期38卷 27-38页

作者： zhe LI Qijun LIU Zhanshan ma Cma Earth System Modeling and Prediction Centre China Meteorological Administration(CMA)Beijing 100081

The horizontal resolution of global numerical weather prediction models is continuously developing. However, due to the imperfect precipitation simulation/forecast of these models, the demand for considering riming particles in cloud microphysical schemes in these models is increasing. This study employed the latest versions of global atmospheric reanalysis data (ERA5), the satellite retrieval data of the Global Precipitation Observation Program (GPM),and station precipitation observations to explore the impacts of adding graupel to the cloud microphysical scheme in the China Meteorological Administration-Global Forecast System (Cma-GFS) on summer regional precipitation simulations in four Chinese climate zones. The results verify that the new graupel scheme can enable Cma-GFS to decently predict global graupel distribution, especially in tropical and midlatitude regions. The addition of graupel in the cloud microphysics increases the precipitation simulation in North China, while that in Southwest China is weakened and dispersed. Moreover, graupel scheme increases the precipitation simulations of almost all *** increase in light rain is obvious, and the absolute value of heavy rain is strengthened. This may be because graupel quickly melts into rain after falling out of the zero-temperature layer due to its large mass and fast falling speed, increasing surface precipitation. In summary, the addition of graupel in the cloud microphysical scheme can improve Cma-GFS’s underestimation of strong precipitation.

关键词： graupel cloud microphysical scheme precipitation China Meteorological Administration-Global Forecast System(Cma-GFS) climate zone

立德树人背景下课程思政元素融入《动物药理学》的教学实践及探索

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现代畜牧科技 2024年第6期 156-158页

作者：云巾宴云鹏马哲李春花尹柏双吉林农业科技学院动物科技学院吉林吉林132101 长春中医药大学护理学院吉林长春130117 吉林省人民医院全科医学科(特诊科) 吉林长春130021

以落实立德树人的育人目标实施课程思政是《动物药理学》的教学任务之一。以《高等学校课程思政建设指导纲要》为指导,挖掘出《动物药理学》教材中的思政元素,分别契合3个思政教育主题:以科技强国为己任,做科技强国践行者;坚持文化自信... 详细信息

以落实立德树人的育人目标实施课程思政是《动物药理学》的教学任务之一。以《高等学校课程思政建设指导纲要》为指导,挖掘出《动物药理学》教材中的思政元素,分别契合3个思政教育主题:以科技强国为己任,做科技强国践行者;坚持文化自信,奔赴崭新征程;珍爱生命,远离毒品。为发挥药理学课程对当代大学生的价值引领作用提供教学参考,为动物医学专业培养符合社会需求的德才兼备的专业人才。

关键词：动物药理学课程思政挖掘实践探索

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Effect of Linear and Ring-like Co-units on the Temperature Dependence of Nucleation and Growth in Ⅱ-Ⅰ Phase Transition of Butene-1 Copolymers

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Chinese Journal of Polymer Science 2018年第11期36卷 1269-1276页

作者： Ya-Hui Lou Yi-Long Liao Li Pan Bin Wang Yue-Sheng Li zhe ma Tianjin Key Laboratory of Composite and Functional materials and School of materials Science and Engineering Tianjin UniversityTianjin 300072China

The phase transition from tetragonal form II to hexagonal form I was studied for the butene-1/ethylene and butene-1/1,5- hexadiene random copolymers, which have comparable molecular weight but distinct linear ethylene and ringlike methylene-1,3- cyclopentane （MCP） structural co-units, respectively. It is known that this solid phase transition follows the nucleation-growth mechanism, so the stepwise annealing protocol was utilized to investigate the influences of co-units on the optimal nucleation and growth temperatures. Compared with optimal nucleation and growth temperatures of-10 and 3 5 ℃, respectively, in polybutene-1 homopolymer, two butene-1/ethylene copolymers with 1.5 mol% and 4.3 mol% co-units have the slightly lower optimal nucleation temperature of-15 ℃ but much higher optimal growth temperature of 50 ℃. Clearly, the effect of ethylene co-unit is more significant on varying optimal temperature for growth than for nucleation. Furthermore, when the incorporated co-unit is ringlike MCP, the optimal nucleation temperature is -15 ℃ for 2.15 mol% co-units, the same with above BE copolymers, but -13 ℃ for a very low concentration of 0.65 mol%. Interestingly, the optimal growth temperature of butene-1/1,5-hexadiene copolymers with 0.65 mo1%-2.15 mol% MCP co- units increases to 55 ℃, which is also independent on co-unit concentration. These obtained values of optimal temperatures provide crucial parameters for rapid II-I phase transition.

关键词： Butene-1 copolymer Co-unit type Phase transformation Nucleation Crystal growth