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Expression Characteristics and Putative Functions of KIF3A/KIF3B During Spermiogenesis of Phascolosoma esculenta

Journal of Ocean University of China 2022年第4期21卷 998-1016页

作者： DU Chen mu danli GAO Xinming LUO Shengyu WANG Jianping JIN Shan ZHU Junquan Key Laboratory of Applied Marine Biotechnology by the Ministry of Education Ningbo UniversityNingbo 315211China Ningbo Academy of Oceanology and Fisheries Ningbo 315012China

The microtubule(MT)-associated proteins KIF3A and KIF3B are ubiquitously expressed in a wide range of *** study investigated the functions of these proteins in spermiogenesis,which involves various MT-dependent processes,in Phasco-losoma *** cloned the complete cDNA of Pe-KIF3A/*** predictions showed that Pe-KIF3A/3B are composed of a highly conserved motor domain,a coiled-coil domain,and a tail ***-time quantitative PCR showed that Pe-kif3a/3b are expressed in all tissues evaluated,with the highest levels in sperm *** in situ hybridization and immunofluo-rescence were employed to analyze the dynamic expression patterns of KIF3A/3B during ***-KIF3A/3B consistently co-localized with MTs at all stages of spermiogenesis,indicating their potential functions in cargo ***-KIF3A/3B co-localized with mitochondria,suggesting that they may mediate mitochondrial *** late-stage spermatids and mature sperm,co-localization was detected in the midpiece,suggesting that Pe-KIF3A/3B could facilitate midpiece *** co-localization of Pe-KIF3A and Pe-KIF3B at all stages of spermiogenesis suggests their function as the heterodimeric *** on the ob-served temporal and spatial expression patterns of Pe-KIF3A/3B,MTs,and mitochondria in our study,we suggest that heterodimer KIF3AB has a potential role in acrosome biogenesis,sperm head remodeling,enflagellation,and mitochondrial migration during spermiogenesis in *** addition,our study on the morphological characteristics of spermatogenic cells provided fundamental data on the reproductive biology of ***.

关键词： Phascolosoma esculenta spermiogenesis kinesin 3A/3B heterodimerization

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Molecular Characterization, Tissue Distribution and Localization of Larimichthys crocea Kif3a and Kif3b and Expression Analysis of Their Genes During Spermiogenesis

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Journal of Ocean University of China 2019年第6期18卷 1451-1469页

作者： mu danli DU Chen FU Suyan WANG Jingqian HOU Congcong TANG Daojun ZHU Junquan The Key Laboratory of Applied Marine Biotechnology of Ministry of Education School of Marine SciencesNingbo University

KIF3A and KIF3B are two N-terminal motor proteins belonging to the kinesin-II superfamily that play essential roles in *** understand the roles played by KIF3 A/3B during spermatogenesis of large yellow croaker Larimichthys crocea,we studied the testis characteristics at different developmental stages of ***,and determined the spatiotemporal expression patterns of kif3a and kif3b during *** real-time PCR(qR T-PCR)showed that the overall trends of kif3 a/3 b m RNA abundance during testis development are *** stage Ⅱ to stage V,kif3a/3b m RNA abundances first increased and then fell after reaching a peak at stage ***,the m RNA abundances of both genes at stage V were higher than those at stages Ⅱ and Ⅲ.In addition,it is worth of noting that kif3 b m RNA abundance was higher than that of kif3a at all *** in situ hybridization results revealed that kif3a/3b m RNA abundance dynamics were consistent with the migration of mitochondria,the deformation of nucleus,and the formation of *** m RNA hybridization signals of both genes first appeared either around the nuclear periphery or on the side of the nuclei,then appeared at one side of nuclei,and finally were mainly on the tail during *** findings contributed to better understanding the molecular mechanisms of spermiogenesis in fish;and suggested that KIF3A and KIF3B may participate in the intracellular transport of mitochondria,nuclear deformation,and the formation of tail during the spermiogenesis in ***.

关键词： Larimichthys crocea kif3a/3b spermiogenesis expression pattern

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Highly sensitive wearable sensor based on a flexible multi-layer graphene film antenna

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Science Bulletin 2018年第9期63卷 574-579页

作者： danli Tang Qianlong Wang Zhe Wang Quantao Liu Bin Zhang Daping He Zhi Wu Shichun mu Hubei Engineering Research Center of Rf-Microwave Technology and Application School of ScienceWuhan University of technologyWuhan 430070China Shenzhen Institute of Advanced Graphene Application and Technology(SIAGAT) Shenzhen 518106China State Key Laboratory of Advanced Technology for Materials Synthesis and Processing Wuhan University of TechnologyWuhan 430070China State Key Laboratory of Silicate Materials for Architectures Wuhan University of TechnologyWuhan 430070China

The use of advanced carbon nanomaterials for flexible antenna sensors has attracted great attention due to their outstanding electromechanical properties. However, carbon nanomaterial based composites have yet to overcome drawbacks, such as low conductivity and toughness. In this work, a flexible multi-layer graphene film(FGF) with a high conductivity of 10~6 S/m for antenna based wearable sensors is investigated. A 1.63 GHz FGF antenna sensor exhibits significantly high strain sensitivity of 9.8 for compressive bending and 9.36 for tensile bending, which is super than the copper antenna sensor(5.39 for compressive bending and 4.05 for tensile bending). Moreover, the FGF antenna sensor shows very good mechanical flexibility, reversible deformability and structure stability, and thus is well suited for applications like wearable devices and wireless strain sensing.

关键词：传感器天线敏感电影压缩弯曲结构稳定性便携设备 FGF

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Low Complexity multiuser Detection with Recursively Successive Zero-Forcing and SIC Based on Nullspace for multiuser MIMO-OFDM System

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China Communications 2015年第9期12卷 53-63页

作者： Zhang Chengwen Wang Bin Li danli Tan Xuezhi The School of Electronics and Information Engineering Harbin Institute of Technology (HIT)

To improve the spectrum efficiency, this paper considers the multiuser detection with the mu-MIMO technology for multiuser MIMO-OFDM system uplink with the same subcarrier shared by multiple users. A low complexity multiuser detection algorithm with recursively successive zero-forcing and successive interference cancellation(RSZF-SIC) based on nullspace is proposed. The RSZF process based on the block diagonalization(BD) technique eliminates the co-channel interference(CCI) by a recursive method based on the nullspace orthogonal theorem. The SIC process detects the user signals respectively with the reasonable user detection sequence based on the results of the RSZF process. The computational complexity of the proposed algorithm is effectively reduced by reducing the total number of singular value decomposition(SVD) operations and the dimension of the SVD matrix in the recursive procedure. The performance of the proposed algorithm is improved in terms of bit error rate and sum capacity of the system, especially in the highSNR regime.

关键词： MIMO-OFDM系统多用户检测算法递归方法低复杂度 SiC 迫零 MIMO技术串行干扰消除

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基于ZigBee网络的TD-SCDMA智能天线信号强度测量系统

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中国测试 2016年第2期42卷 75-78页

作者：穆维新施俊申金媛刘润杰寇丹丽郑州大学信息工程学院河南郑州450001

由于智能天线的波束成形技术,传统测量电磁辐射的方法已不适用于TD-SCDMA智能天线。该文设计一种TD-SCDMA智能天线的信号强度测量系统,根据智能天线的特点,应用ZigBee网络同时获取多个测量点的信号强度值。另外,还提供一种测量点的布设... 详细信息

由于智能天线的波束成形技术,传统测量电磁辐射的方法已不适用于TD-SCDMA智能天线。该文设计一种TD-SCDMA智能天线的信号强度测量系统,根据智能天线的特点,应用ZigBee网络同时获取多个测量点的信号强度值。另外,还提供一种测量点的布设方案,并使用该方案进行实际测量。实际测量结果显示:设计的测量系统的测量精度高于传统的测量方法,进而可以更准确地进行电磁环境的测评,具有较强的实用价值。

关键词： TD-SCDMA 智能天线 ZigBee网络信号强度测量系统

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高士奇《江村书画目》研究

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南京艺术学院学报（美术与设计） 2010年第6期 26-30页

作者：李万康谭丹莉南京艺术学院人文学院江苏南京210013

《江村书画目》是一本书画方面的珍贵账簿,作者是高士奇,传录者是高舆。整个账簿分十类,其中"进字壹号"和"进字贰号"录有大量赝品,但迄今没有证据表明高氏父子有意以赝品欺骗康熙,所谓高士奇以"以赝品欺罔"的定论是不成立的。在整个《... 详细信息

《江村书画目》是一本书画方面的珍贵账簿,作者是高士奇,传录者是高舆。整个账簿分十类,其中"进字壹号"和"进字贰号"录有大量赝品,但迄今没有证据表明高氏父子有意以赝品欺骗康熙,所谓高士奇以"以赝品欺罔"的定论是不成立的。在整个《江村书画目》中,"永存秘玩上上神品"、"自题上等手卷"是核心,这两类珍贵字画在高士奇去世之后相继失散,多数入宫。从《江村书画目》的备注和购价可以见出,高士奇具有卓越的鉴赏能力和衡价水平。

关键词：高士奇高舆赝品字画价格

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单双弧体部立体定向放射治疗计划对早期肺癌治疗剂量、效率的预测效果比较

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山东医药 2016年第39期56卷 47-49页

作者：张基永洪丹丽吴丽丽陆佳扬汕头大学医学院附属肿瘤医院广东汕头515000 汕头大学医学院第一附属医院

目的比较无均整滤过器模式单、双弧体部立体定向放射治疗计划对早期肺癌的剂量和效率的预测结果。方法选取18例早期肺癌患者,先行胸部CT检查,将CT影像导入Eclipse10.0计划系统,分别设计无均整滤过器模式6 MV X射线双弧体部立体定向放射... 详细信息

目的比较无均整滤过器模式单、双弧体部立体定向放射治疗计划对早期肺癌的剂量和效率的预测结果。方法选取18例早期肺癌患者,先行胸部CT检查,将CT影像导入Eclipse10.0计划系统,分别设计无均整滤过器模式6 MV X射线双弧体部立体定向放射治疗计划(D-RA)和单弧体部立体定向放射治疗计划(S-RA),处方剂量48 Gy,12 Gy/次,照射4次。比较D-RA、S-RA计划95%的处方剂量覆盖靶区体积的百分数(V95%)、90%的处方剂量覆盖靶区体积的百分数(V90%)、105%的处方剂量覆盖靶区体积的百分数(V105%)、适形指数(CI)、均匀指数(HI)、危机器官(OAR)受照剂量、计划设计时间(PT)、机器跳数(mu)、治疗时间(TT)。结果 D-RA、S-RA的V90%、V95%、V105%间比较,P均>0.05。D-RA、S-RA的CI、TT间比较,P0.05。D-RA、S-RA的PT、mu间比较P均>0.05。结论无均整滤过器模式DRA、S-RA计划的照射剂量均能覆盖早期肺癌患者的肿瘤靶区,各OAR受照剂量均满足限量要求。D-RA和S-RA计划的靶区覆盖情况及各OAR受照剂量差异较小。D-RA的适形性和均匀性均优于S-RA,S-RA治疗时间短、机器跳数少。

关键词：单弧体部立体定向放射治疗双弧体部立体定向放射治疗无均整滤过器模式肺癌肺肿瘤

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