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Engineered Regeneration 2022年第3期3卷 283-291页

作者： Anju ms Athira RK Ramesh Babu V Anil Kumar PR Naresh Kasoju Division of Tissue Culture Department of Applied BiologyBiomedical Technology WingSree Chitra Tirunal Institute for Medical Science and TechnologyThiruvananthapuram 695012KeralaIndia Division of Precision Fabrication Department of Medical Devices EngineeringBiomedical Technology WingSree Chitra Tirunal Institute for Medical Science and TechnologyThiruvananthapuram 695012KeralaIndia

Temporary storage/shipping of cell/tissue engineering products from bench to bedside is a key aspect of re-generative *** current proof-of-concept study presents a multipurpose device for temporary storage/shipping of cell culture dishes containing cell/tissue *** device,made with readily available raw ma-terials,contains three elements viz.a specialized lid,polymeric plates having grooves and a set of nuts and *** part of the performance evaluation,the device was first subjected to a simulated storage/shipping process,wherein the leak-proof and aseptic containment of the contents was ***,the setup was used for temporary storage/shipping of dishes having(a)L929 cell monolayers cultured on treated surfaces,(b)SIRC,HaCaT and A549 cell sheets cultured on thermo-responsive surfaces,(c)HOS-cell encapsulated agar gels and(d)HOS-cell seeded silk fibroin *** results showed that the health of cell monolayers/cell sheets/tissue constructs after the process was comparable to that before the *** device was scalable,simple to handle,can be made for a single or multi-use purpose,and can be resizable to load other culture *** design of the storage/shipping device described in this report thus offers versatile features and applications.

关键词： Tissue engineering Scaffolds Tissue equivalents Translational medicine

Residual effects of TMOF-Bti formulations against 1^(st) instar Aedes aegypti Linnaeus larvae outside laboratory

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Asian Pacific Journal of Tropical Biomedicine 2012年第4期2卷 315-319页

作者： Saiful AN Lau ms Sulaiman S Hidayatulfathi O Biomedical Science Programme Faculty of Health ScienceUniversiti Kebangsaan MalaysiaKampus Kuala LumpurJalan Raja Muda Abdul Aziz

Objective:To evaluate the effectiveness and residual effects of trypsin modulating ecstatic factor-Bacillus thuringiensis israeliensis(TMOF-Bti) formulations against Aedes aegypti(Ae. aegypti)(L) larvae at UKM Campus Kuala ***:Twenty first instar *** larvae were added in each bucket containing 4 L of water supplied with crushed dried-leaf powder as their source of *** of TMOF-Bti in rice husk formulation with the following weights viz 10,25,50 and 100 mg,respectively in duplicate was distributed in the buckets;while TMOF-Bti in wettable powder formulation each weighing viz 2,5,10 and 20 mg,respectively in duplicate was also placed in the *** control buckets run in duplicate with 4 L of water and 20 first instar *** *** buckets were covered with mosquito *** mortality was recorded after 24 hours and weekly for five weeks.A new batch of 20 1^(st) instar larvae *** was introduced into each bucket weekly without additional TMOF-Bti rice husk formulation or wettable *** experiment was repeated for four ***:The result of the study showed that all formulations were very effective on the first two weeks by giving 100% larval mortality for all concentrations *** TMOF(2%) + Bti(2%) had a good residual effect until the end of 3^(rd) week,TMOF(4%) + Bti(4%) until 4^(th) week,wettable powder TMOF(20%) + Bti(20%) until the third ***:From the results it can be concluded that the TMOF-Bti formulations can be utilized in dengue vector control.

关键词： TMOF-Bti formulations 1^(st) instar Aedes aegypti Outside laboratory Residual effect Larvae Dengue Dengue vector control Mosquito Larval mortality

P-wave velocities of alkaline olivine basalt at high pressure and temperature and its controlling factors

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Chinese Science Bulletin 1997年第9期42卷 761-764页

作者： Song, ms Xie, HS Zhang, YM Hou, W Xu, J Xu, YS Earth’s Interior Material Laboratory Institute of Geochemistry Chinese Academy of Sciences Guiyang China Institute of Earth Science Academia Sinica Taibei China

SEISMIC wave inversion is one of the most important means for us to recognize the compositionand structure of the Earth’s *** the experimental techniques of elastic wave ve-locity measurement at in situ high pressure... 详细信息

关键词： high pressure and temperature alkaline olvine basalt P-wave velocity softening effect phase transformation.

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Measurement of electrical conductivity of 0.001 mol NaCl solution under high pressures

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Chinese Science Bulletin 1997年第18期42卷 1563-1566页

作者： Zheng, HF Xie, HS Xu, YS Song, ms Guo, J Zhang, YM Department of Geology Peking University Beijing China Institute of Geochemistry Chinese Academy of Sciences Guiyang China

The experiments were carried out in a YJ-3000 ton press fitted with a wedge-type cubic anvil. The samples were pressed using pyropgyllite as the pressure medium. The conductance cell for the experiments was made of Te... 详细信息

关键词： electrical conductivity high pressure NaCI solution.

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Prevalence and antimicrobial resistance pattern of bacterial strains isolated from patients with urinary tract infection in Messalata Central Hospital, Libya

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Asian Pacific Journal of Tropical Medicine 2016年第8期9卷 749-754页

作者： Mahmoud A.Mohammed Tarig ms Alnour Osama M.Shakurfo Mariam M.Aburass Department of Medical Laboratory Technology Faculty of Medical Technologies-Messalata Almergib University Department of Food Hygiene and Control Faculty of Veterinary Medicine Mansoura University Department of Medical Laboratory Technology Faculty of Medical Technologies-MessalataAlmergib University Department of Medical Laboratory Technology Faculty of Applied Medical Sciences University of Tabuk

Objective: To investigate the prevalence of urinary tract infection among patients at Messalata Central Hospital, Libya, to identify the causative bacteria, and to explore their resistance pattern to antimicrobials. Methods: A total number of 1 153 urine samples were collected from patients, who attended daily to Messalata Central Hospital, Libya, in a study extended for one year. Antimicrobial susceptibility testing and isolates typing were done using Phoenix BD(BD diagnostic). Resistance was confirmed manually using agar disk diffusion method. Results: Of the 1 153 urine samples tested, 160(13.9%) samples were positive, from which 17 different, solely Gram negative, uropathogens were identified. Escherichia coli were the most prevalent(55.6%) bacteria, followed by Klebsiella pneumoniae subspecies pneumoniae(16.3%), Proteus mirabilis(6.3%), Pseudomonas aeruginosa(5.6%), Enterobacter cloacae and Klebsiella oxytoca(2.5%, each), Citrobacter koseri and Providencia rettgeri(1.9%, each), Acinetobacter baumannii, Enterobacter aerogenes and Proteus vulgaris(1.3%, each), and Aeromonas caviae, Citrobacter freundii, Cronobacter sakazakii, Enterobacter amnigenus biogroup 2, Pseudomonas putida and Serratia marcescens(0.6%, each). The isolated uropathogens showed increased levels of resistance ranged from 10.5% to 64.5%, with an overall resistance of 28.9%. Amikacin was the most effective antimicrobial followed by Imipenem and Meropenem(0%, 0.6% and 2.5% resistance, respectively); while, Cephalothin and Ampicillin were the least(80.6% and 90.0% resistance, respectively) effective. Conclusions: The obtained results emphasized the emergence of highly resistant bacteria to most of tested antimicrobials and raise the alarm for physicians to change their treatment pattern depending on antimicrobial susceptibility results.

关键词： Urinary tract infection(UTI) Uropathogens Significant Bacteruria Antimicrobial susceptibility testing(AST) Antimicrobial resistance Enterobacteriaceae

Patterns of cancer recurrence in localized resected hepatocellular carcinoma

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Hepatobiliary & Pancreatic Diseases International 2015年第3期14卷 269-275页

作者： Aryavarta ms Kumar Elisha T Fredman Christopher Coppa Galal El-Gazzaz Federico N Aucejo May Abdel-Wahab Department of Radiation Oncology 9500 Euclid AveT28 Cleveland ClinicCleveland OH 44195 USA Department of Radiology 9500 Euclid Ave Hb6 Cleveland ClinicCleveland OH 44195 USA Department of Hepato-pancreato-biliary & Transplant Surgery 9500 Euclid Ave A100Cleveland OH 44195 USA

BACKGROUND： Tumor resection in non-metastatic hepato- cellular carcinoma （HCC） patients with adequate liver reserve offers a potential cure, but has a high 5-year recurrence rate. We analyzed the patterns of cancer... 详细信息

关键词： hepatocellular carcinoma patterns of failure partial hepatectomy

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Rotula aquatica Lour. inhibits growth and biofilm formation of clinically isolated uropathogenic Escherichia coli

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Asian Pacific Journal of Tropical Biomedicine 2020年第12期10卷 547-554页

作者： A.Vysakh Sebastian Jose Midhun Ninan Jisha Kuriakose Jayesh V.Vijeesh Mathew Jyothis ms Latha School of Biosciences Mahatma Gandhi UniversityPriyadarshini HillsKottayamKeralaIndia

Objective:To evaluate the anti-bacterial and anti-biofilm activity of ethyl acetate fraction of Rotula aquatica Lour.(EFRA)against clinically isolated uropathogenic Escherichia ***:In vitro antibacterial and anti-biofilm studies were *** antimicrobial activity of EFRA was assayed by the well diffusion *** minimum inhibitory concentration(MIC)and minimum bactericidal concentration(MBC)of the active fraction were determined by Resazurin *** time-kill kinetic assay,acridine orange-ethidium bromide staining,propidium iodide uptake assay,and scanning electron microscopic(SEM)analysis were done to evaluate the efficacy of EFRA in killing uropathogenic Escherichia *** anti-biofilm activity was determined by 3-[4,5-dimethyl-2-thiazolyl]-2,5-diphenyl-2 H-tetrazolium-bromide(MTT)assay and specific biofilm formation ***:The well diffusion assay of EFRA showed a very clear zone of inhibition against Escherichia coli *** MIC and MBC of EFRA were 2.5 mg/m L and 5 mg/m L,*** time-kill kinetic assay,fluorescence microscopic analysis,propidium iodide uptake assay,and SEM analysis displayed the effect of EFRA in killing the *** MTT assay and specific biofilm formation assay showed that EFRA prevented the formation of ***:The results of the present study confirm that EFRA could prevent bacterial growth and inhibit its biofilm formation.

关键词： Rotula aquatica Escherichia coli Urinary tract infections Anti-bacterial activity

Effects of Mirazid~ and myrrh volatile oil on adult Fasciola gigantica under laboratory conditions

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Asian Pacific Journal of Tropical Biomedicine 2012年第11期2卷 875-884页

作者： AM Massoud HA Shalaby RM El Khateeb ms Mahmoud MA Kutkat Tropical Medicine Department Faculty of MedicineAl-Azhar University Parasitology and Animal Diseases Department National Research CenterGizaEgypt Poultry Diseases Department National Research CenterGizaEgypt

Objective:To evaluate the effects of Mirazid and myrrh volatile oil on adult Fasciola gigantica(***) under laboratory ***:The effects of oleoresin extract of myrrh(Mirazid) and myrrh volatile oil on the surface morphology of adult *** following treatment in vitro had been determined by scanning electron *** results were compared with those observed in the fluke tegument following incubation in triclabendazole sulphoxide(TCBZ-SO),active form.(Fasinex,Ciba-Geigy).Results:Observations of the efficacy of Mirazid oleoresin extract and myrrh volatile oil indicated that both products showed dosedependent anthelmintic *** anterior half of the fluke was consistently more severely affected than the posterior *** surface changes induced by Mirazid oleoresin extract were less severe than those observed after exposure to either myrrh volatile oil or *** showed swelling after these treatments,but its level and blebbing were much greater with myrrh volatile oil;in which patches of tegumental sloughing were observed in the apical cone and the posterior mid-body region of *** was not observed after treatment with Mirazid oleoresin ***:The comparatively more disruption,observed in myrrh volatile oil exposed specimens,compared to that exposed to Mirazid oleoresin extract might suggest that the anthelmintic activity of Mirazid oleo resin extract was attributed to its content of volatile ***,increasing the concentration of myrrh volatile oil in Mirazid might possibly help to developing its anthelmintic activity._______________________________________________

关键词： Fasciola gigantica Mirazid Myrrh volatile oil In vitro effect

Getting to Know Animals

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英语大王 2013年第Z2期 10-15页

作者： ms Edit Dobos

Flora is seven and a half years old. She lives with her parents in the city. This summer, she visited the countryside for the first time.* She lived there with Aunt Agnes and Uncle Tony. What adventures(冒险经历)did s... 详细信息

关键词：小助手 Getting to Know Animals

Identification and analysis of genes differentially expressed in the Spodoptera litura fat body in response to the biocontrol fungus,Nomuraea rileyi

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Identification and analysis of genes differentially expresse...