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Mitochondrial DNA diversity and origin of indigenous pigs in South China and their contribution to western modern pig breeds

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Journal of Integrative Agriculture 2019年第10期18卷 2338-2350页

作者： WANG Chen CHEN Yao-sheng HAN Jian-lin mo de-lin LI Xiu-jin LIU Xiao-hong State Key Laboratory of Biocontrol School of Life SciencesSun Yat-sen UniversityGuangzhou 510006P.R.China CAAS-ILRI Joint Laboratory on Livestock and Forage Genetic Resources Institute of Animal ScienceChinese Academy of Agricultural SciencesBeijing 100193P.R.China International Livestock Research Institute (ILRI) Nairobi 00100Kenya

Indigenous pigs in South China are valuable genetic resources with many specific and unique characters, which have played an important role in the establishment of some western modern pig breeds. However, the origin and genetic diversity of indigenous pigs in South China have not been fully understood. In the present study, we sequenced 534 novel mitochondrial DNA (mtDNA) D-loop and assembled 54 complete mitogenome sequences for all 17 indigenous pig breeds from Fujian, Guangdong, Guangxi and Hainan in South China. These data were analyzed together with previously published homologous sequences relevant to this study. We found that all 13 coding genes of the mitogenomes were under purifying selection, but ND1 had the most variable sites and CYTB contained the most non-synonymous SNPs. Phylogenetic analysis showed that all indigenous pigs in South China were clustered into the D haplogroup with D1a1, D1b, D1c and D1e sub-haplogroups found to be dominant. Haplotype and nucleotide diversities of D-loop sequences ranged from 0.427 to 0.899 and from 0.00342 to 0.00695, respectively, among which all pigs in Guangdong had the lowest diversity. The estimates of pairwise FST, gene flow (Nm) and genetic distance (Da) indicated that most of these indigenous pig breeds differentiated from each other significantly (Pmong the western modern breeds, Berkshire and Yorkshire had significant Asian matrilineal footprints from indigenous pigs in South China, especially the Spotted pigs distributed in Guangdong and Guangxi. The neutrality test (Fu’s FS) indicated that indigenous pigs from Fujian and Guangxi had gone through recent population expansion events (Pded that indigenous pigs in South China were most likely derived from the Mekong region and the middle and downstream regions of Yangtze River through Guangxi and Fujian. Our findings provide a complete and in-depth insight on the origin and distribution pattern of maternal genetic diversity of indigen

关键词： pig South China mitogenome D-loop genetic diversity

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Effects of back fat,growth rate,and age at first mating on Yorkshire and Landrace sow longevity in China

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Journal of Integrative Agriculture 2016年第12期15卷 2809-2818页

作者： HU Bin mo de-lin WANG Xiao-ying LIU Xiao-hong CHEN Yao-sheng State Key Laboratory of Biocontrol School of Life SciencesSun Yat-Sen University

In the present study, genetic parameters for longevity and genetic correlations with back fat （BF）, average daily gain （ADG）, and first mating age （FMA） were estimated from 19 300 Yorkshire and 18 378 Landrace sows from 12 farms derived from one breeding system. General linear models （GLM） were employed to estimate genetic correlations with heritability （h2）. Longevity was defined by six different parameters （i.e., traits） and heritability ranged from h2=0.13 to 0.16 （P〈0.05） in both swine breeds. Genetic correlations between different longevity definitions were positive and high （r=0.79 to 0.99; P〈0.05）. BF exhibited low positive genetic correlations （r=0.12 to 0.15; P〈0.05） with longevity traits identified in both pig breeds which were dependent on trait definition, whereas ADG and FMA showed weak negative genetic correlations with longevity traits. The influence of BF, ADG, and FMA on longevity traits was examined by dividing the production and reproductive traits into several numerical groups; the influence of each longevity trait was subsequently analyzed among the different groups. The results indicated BF was the best trait to improve longevity among BF, ADG, and FMA, which showed positive effects for almost all longevity traits. For Yorkshire sows, gilts with over 18 mm BF were most desirable; Landrace with 10.6-13 mm BF were suitable for longevity. ADG was not favorable for gilt selection, because the correlation between growth rate and longevity very low. For FMA, our results indicated gilts mating before 230 days exhibited a longer life span. Therefore, we suggest farmers should select for thicker back fat and mate at an earlier age to improve sow longevity.

关键词： sow longevity back fat average daily gain first mating age

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Investigation of Genetic Variation of B-G Gene with PCR-SSCP and RFLP in Chinese Indigenous Chickens

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Agricultural Sciences in China 2005年第8期4卷 621-628页

作者： XU Ri-fu LI Kui CHEN Guo-hong QIANG-BA Yang-zong XU Hai mo de-lin LI Chang-chun FAN Bin LIU Bang Laboratory of molecular Biology and Animal Breeding College of Animal Science and Technology Huazhong Agricultural University Wuhan 430070 P.R.China Institute of Animal Science Chinese Academy of Agricultural Sciences Beijing 100094 P.R. China department of Animal Science and Technology Yangzhou University Yangzhou 225009 P.R.China department of Animal Husbandry and Veterinary College of Agriculture and Animal Husbandry Tibetan University Linzhi 860000 P. R. China

To reveal genetic variation of MHC B-G gene at Chinese native chickens, two PCR primer pairs were designed to hybridize specifically with conserved sequences surrounding hypervariable regions within the B-G gene and used to amplify two DNA fragments in ten Chinese indigenous chicken breeds and one introduced breed. The fragments were cloned and sequenced to assure that the expected sequences of chicken B-G gene were isolated. Of which the 189 bp fragment encompassing the most variable region within exon 2 of B-G gene was employed for PCR-SSCP assay, this method provided evidence for the presence of at least 56 B-G genotypes in the Chinese chickens sampled. It revealed a high degree of diversity in B-G genes of Chinese local breeds; particularly, high variation of B-G gene was confirmed with the presence of 48 B-G genotypes within Tibetan chicken population. Not only can the B-G genotypes be used to preliminarily screen new B-G alleles, but also they would be utilized to investigate MHC haplotypes and matched unrelated donors for bone marrow transplantation in immune researches. Another fragment of 401 bp size spanning over partial intron 1 and exon 2 of B-G gene was employed for PCR-RFLP analysis with two restriction enzymes of Msp Ⅰ and Tas Ⅰ in the breeds sampled. In this part of the gene, three novel SNPs were detected at the two restriction sites. It was more generally found the transition of two nucleotides of A294G and T295C occurred at Tas Ⅰ restriction site, and consequently led to a non-synonymous substitution of asparagine into serine at position 54 within the deduced amino acid sequence of immunoglobulin variable-region-like domain encoded by the exon 2 of B-G gene. It was observed at rare frequency that an alone mutation of A294G occurring at the site, which also caused the substitution of amino acid, asparagine 54-to-serine; and we haven＇t found only single mutation occurred at position 295 of the restriction site. At the Msp Ⅰ site, the transversion o

关键词： B-G genes PCR-SSCP assay Genetic variation Chinese indigenous chicken Genotype Allele

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全基因组选择在猪育种中的应用

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畜牧兽医学报 2016年第1期47卷 1-9页

作者：王晨秦珂薛明莫德林陈瑶生刘小红中山大学生命科学学院广州510006 全国畜牧总站北京100125

全基因组选择(Genomic selection,GS)是一种全基因组范围内的标记辅助选择方法。利用全基因组遗传标记信息对个体进行遗传评估,能够更加准确地早期预测估计育种值,降低近交系数,大大提高猪育种的遗传进展。随着猪全基因组测序的完成和猪... 详细信息

全基因组选择(Genomic selection,GS)是一种全基因组范围内的标记辅助选择方法。利用全基因组遗传标记信息对个体进行遗传评估,能够更加准确地早期预测估计育种值,降低近交系数,大大提高猪育种的遗传进展。随着猪全基因组测序的完成和猪60kSNP芯片的商业化,全基因组选择已经成为猪育种研究领域的新热点。本文综述了全基因组选择的分析方法、计算方法和影响因素,并阐述了全基因组选择在猪育种中的应用情况和发展趋势。

关键词：全基因组选择猪育种 SNP芯片

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PI3K/AKT抑制剂渥曼青霉素对猪前体脂肪细胞增殖和凋亡的影响

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遗传 2012年第10期34卷 1282-1290页

作者：郭云学莫德林陈瑶生张悦张云肖书奇刘小红中山大学生命科学学院有害生物控制与资源利用国家重点实验室广州510006

为探寻PI3K/AKT抑制剂渥曼青霉素(Wortmannin,WM)对猪前体脂肪细胞增殖和凋亡均无影响的适宜浓度,文章首先分离并验证了猪原代前体脂肪细胞的分化潜能,然后对不同浓度渥曼青霉素处理11 d的细胞采用Annexin V-FITC/PI双标法检测细胞凋亡... 详细信息

为探寻PI3K/AKT抑制剂渥曼青霉素(Wortmannin,WM)对猪前体脂肪细胞增殖和凋亡均无影响的适宜浓度,文章首先分离并验证了猪原代前体脂肪细胞的分化潜能,然后对不同浓度渥曼青霉素处理11 d的细胞采用Annexin V-FITC/PI双标法检测细胞凋亡,并通过凋亡相关基因的表达以及DNA损伤程度进行验证,同时利用甲烷硫代磺酸盐(Methanethiosulfonate,MTS)检测了细胞的增殖活性。结果表明,100 nmol/L渥曼青霉素对猪前体脂肪细胞的增殖和凋亡均无显著影响,而200 nmol/L的渥曼青霉素对猪原代脂肪细胞的增殖活性虽没有显著影响,但对细胞凋亡有显著促进作用。研究发现,处理后促凋亡因子caspase8和TNFR1表达显著上调,非caspase依赖促凋亡因子GZMA表达无显著性差异,而GZMB表达则显著上调,抗凋亡因子Bcl-x1表达显著上调,cFLIP表达则无显著性差异。100 nmol/L的渥曼青霉素对细胞DNA的损伤不显著。因此,100 nmol/L的渥曼青霉素对猪前体脂肪细胞的增殖和凋亡均无显著影响,是在不影响细胞生长的情况下研究PI3K通路对脂肪细胞分化的较为理想的浓度。

关键词：渥曼青霉素脂肪细胞增殖凋亡

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Multiple liver metastases of unknown origin:A case report

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World Journal of Gastrointestinal Oncology 2025年第1期17卷 233-238页

作者： Ying-Jin Wang Ze-Chuan Liu Jian Wang Yin-mo Yang department of Hepatobiliary Pancreatic Surgery Peking University First HospitalBeijing 100034China department of Interventional and Vascular Surgery Peking University First HospitalBeijing 100034China

BACKGROUND The liver is the most common site of digestive system tumor metastasis,but not all liver metastases can be traced back to the primary *** it is unusual,syphilis can impact the liver,manifesting as syphilitic hepatitis with inflammatory nodules,which might be misdiagnosed as *** SUMMARY This case report involves a 46-year-old female who developed right upper abdominal pain and intermittent low fever that persisted for more than three *** definitive diagnosis of a tumor had been made in the past decades,but signs of multiple liver metastases were recognized after a computed tomo-graphy scan without evidence of primary *** positive serological tests for syphilis and a biopsy of the liver nodules,a diagnosis of hepatic syphilis was made and confirmed with follow-up nodule reduction after anti-syphilis *** Clinicians must be aware of the possibility that syphilis can cause hepatic inflam-matory masses,especially when liver metastasis is suspected without evidence of primary lesions.A definitive diagnosis should be established in conjunction with a review of the patient’s medical history for accurate therapeutic intervention.

关键词： Syphilitic hepatitis Liver nodules Liver metastasis Treponema pallidum Case report

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长白猪×蓝塘猪及其杂种基因组DNA甲基化分析

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畜牧兽医学报 2011年第5期42卷 613-620页

作者：肖正中刘小红王翀李加琪莫德林陈瑶生华南农业大学动物科学学院广州510642 广东韶关学院农业科学系韶关512005 中山大学生命科学学院广州510006

本研究旨在分析猪基因组DNA胞嘧啶甲基化模式和程度以及父母代猪与其杂种F1代之间的甲基化差异。应用甲基化敏感扩增多态性(MSAP)技术对6头长白公猪、50头蓝塘母猪及长×蓝51头杂交F1代3个群体共107个个体耳组织基因组DNA胞嘧啶甲基化... 详细信息

本研究旨在分析猪基因组DNA胞嘧啶甲基化模式和程度以及父母代猪与其杂种F1代之间的甲基化差异。应用甲基化敏感扩增多态性(MSAP)技术对6头长白公猪、50头蓝塘母猪及长×蓝51头杂交F1代3个群体共107个个体耳组织基因组DNA胞嘧啶甲基化模式和程度进行评估。应用筛选的20对引物扩增,总共得到1 074条带(CCGG位点),共得到415个甲基化位点(条带),其中79条带为甲基化多态性条带,平均甲基化多态性比率(p)达7.4%。结果显示,3个猪群中DNA甲基化程度高,且亲代和F1代杂种之间的甲基化水平存在差异,父母代及其杂交F1代3个群体基因组DNA甲基化总体水平分别是27.7%、27.8%和25.1%,且3个猪群中CCGG序列发生单链DNA外部胞嘧啶甲基化现象(20.8%)比单双链DNA内部胞嘧啶甲基化现象(17.9%)多。结果提示,使用MSAP技术检测猪种基因组甲基化多态性非常有效,且猪基因组甲基化多态性丰富,杂种F1代与其父母代之间的基因组甲基化水平存在差异。

关键词：猪 DNA 甲基化 MSAP

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Curcumin in gastric cancer treatment:A commentary on mechanistic insights and future directions

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World Journal of Gastrointestinal Oncology 2025年第1期17卷 265-267页

作者： Xin-Yue Wei Wen-Bo Cao Sai-Jun mo Zhi-Yan Sun department of Pathophysiology School of Basic Medical SciencesZhengzhou UniversityZhengzhou 450001Henan ProvinceChina School of Basic Medical Science Zhengzhou UniversityZhengzhou 450001Henan ProvinceChina department of Basic Science of Oncology School of Basic Medical SciencesZhengzhou UniversityZhengzhou 450001Henan ProvinceChina department of Special Service No.988 Hospital of the Joint Service Support Force of PLAZhengzhou 450042Henan ProvinceChina

The study by Yang et al presents a comprehensive investigation into the thera-peutic potential of curcumin for gastric cancer(GC).Using network pharma-cology,the researchers identified 48 curcumin-related genes,31 of which overlap with GC *** genes,including ESR1,EGFR,CYP3A4,MAPK14,CYP1A2,and CYP2B6,are linked to poor survival in GC *** docking con-firmed strong binding affinity of curcumin to these *** vitro experiments demonstrated that curcumin effectively inhibits the growth and proliferation of BGC-823,suggesting its therapeutic potential in GC through multiple targets and pathways.

关键词： Curcumin Gastric cancer Network pharmacology Mechanism of action In vitro experiments

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鸡MHC B-LBⅡ新等位基因检测及多态性研究

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畜牧兽医学报 2005年第12期36卷 1247-1255页

作者：徐日福李奎陈国宏强巴央宗莫德林李长春樊斌刘榜华中农业大学动物科技学院分子生物学与动物育种教研室武汉430070 扬州大学动物科技学院扬州225009 西藏大学农牧学院畜牧兽医系林芝860000

在鸡的基因组中扩增了MHC B-LBⅡ基因第2外显子长度为374bp的片段,通过克隆、测序,发现了20个B-LBⅡ新等位基因.对第2外显子6个限制性酶切位点(AluⅠ、CaiⅠ、CfrⅠ、Hinl Ⅰ、HinfⅠ和RsaⅠ)的PCR-RFLP多态性进行分析,并运用在这6个位... 详细信息

在鸡的基因组中扩增了MHC B-LBⅡ基因第2外显子长度为374bp的片段,通过克隆、测序,发现了20个B-LBⅡ新等位基因.对第2外显子6个限制性酶切位点(AluⅠ、CaiⅠ、CfrⅠ、Hinl Ⅰ、HinfⅠ和RsaⅠ)的PCR-RFLP多态性进行分析,并运用在这6个位点所观测的纯合基因型组合对B-LBⅡ等位基因进行初步检测,结果显示,该方法的等位基因检出率可达65%,等位基因主型检出率为68.75%.对B-LBⅡ基因7个单倍型和20个等位基因第2外显子多态性分析表明,核苷酸的多态变异位点为63个,其中简约性信息位点48个,单变异位点15个;仅在中国地方鸡种所检测到的简约性信息位点13个,单变异位点11个.序列同源性达90.6%～99.5%;核苷酸异义替换率为14.64%±2.67%,高于同义替换率2.92%±0.94%.为鸡B-LBⅡ基因多态性进化机制研究提供了参考资料.

关键词：等位基因中国地方鸡种B-LBⅡ基因多态性

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交配组合和环境温度对西藏地区鸡蛋孵化效果的影响分析

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畜牧兽医学报 2006年第1期37卷 28-33页

作者：莫德林李奎强巴央宗唐晓惠朱猛进徐日福樊斌刘榜华中农业大学动物科技学院武汉430070 西藏大学农牧学院牧医系林芝860000

为提高外来高产鸡品种在高原地区的种蛋孵化率,改良当地藏鸡的生产和繁殖性能,在西藏环境条件下以藏鸡和引进隐性白羽鸡为亲本设立4种交配组合,对其繁殖性状及影响因素进行了分析,结果表明:以藏鸡为母本的种蛋受精率显著低于隐性白羽鸡(... 详细信息

为提高外来高产鸡品种在高原地区的种蛋孵化率,改良当地藏鸡的生产和繁殖性能,在西藏环境条件下以藏鸡和引进隐性白羽鸡为亲本设立4种交配组合,对其繁殖性状及影响因素进行了分析,结果表明:以藏鸡为母本的种蛋受精率显著低于隐性白羽鸡(P<0.05),但其孵化率显著高于后者(P<0.05),尤其在孵化后期母本为隐性白羽鸡的胚胎死亡率比藏鸡高7.95%。而母本为藏鸡的杂交组合Ⅲ,其孵化率比母本为隐性白羽鸡的杂交组合Ⅱ高20.44%,为以藏鸡为母本进行杂交改良提供了理论依据。Binary logistic回归分析结果进一步表明,交配组合与环境温度是影响孵化率的重要因素。

关键词：藏鸡隐性白羽鸡交配组合胚胎死亡率受精率孵化率

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