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Regulatory role of NFAT1 signaling in articular chondrocyteactivities and osteoarthritis pathogenesis

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BIOCELL 2023年第10期47卷 2125-2132页

作者： mingcai zhang TANNER CAMPBELL SPENCER FALCON JINXI WANG Harrington Laboratory for Molecular Orthopedics Department of Orthopedic SurgeryUniversity of Kansas Medical CenterKansas CityUSA

Osteoarthritis (OA), the most common form of joint disease, is characterized clinically by joint pain, stiffness,and deformity. OA is now considered a whole joint disease;however, the breakdown of the articular cartilage remains themajor hallmark of the disease. Current treatments targeting OA symptoms have a limited impact on impeding orreversing the OA progression. Understanding the molecular and cellular mechanisms underlying OA development isa critical barrier to progress in OA therapy. Recent studies by the current authors’ group and others have revealedthat the nuclear factor of activated T cell 1 (NFAT1), a member of the NFAT family of transcription factors, regulatesthe expression of many anabolic and catabolic genes in articular chondrocytes of adult mice. Mice lacking NFAT1exhibit normal skeletal development but display OA in both appendicular and spinal facet joints as adults. Thisreview mainly focuses on the recent advances in the regulatory role of NFAT1 transcription factor in the activities ofarticular chondrocytes and its implication in the pathogenesis of OA.

关键词： Osteoarthritis Chondrocyte NFAT1 Transcription factor Regulation of gene expression

Epigenetic regulation of gene expression in osteoarthritis

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Genes & Diseases 2015年第1期2卷 69-75页

作者： mingcai zhang Jinxi Wang Harrington Laboratory for Molecular Orthopedics Department of Orthopedic SurgeryUniversity of Kansas Medical CenterKansas CityKSUSA Department of Biochemistry&Molecular Biology University of Kansas Medical CenterKansas CityKSUSA

Osteoarthritis(OA)is the most common form of joint disease and the leading cause of chronic disability in middle-aged and older *** development of diseasemodifying therapy for OA currently faces major obstacles largely because the regulatory mechanisms for the function of joint tissue cells remain *** studies have found that the alterations in gene expression of specific transcription factors(TFs),pro-or antiinflammatory cytokines,matrix proteinases and extracellular matrix(ECM)proteins in articular cartilage may be involved in the development of ***,the regulatory mechanisms for the expression of those genes in OA chondrocytes are largely *** recent advances in epigenetic studies have shed light on the importance of epigenetic regulation of gene expression in the development of *** this review,we summarize and discuss the recent studies on the regulatory roles of various epigenetic mechanisms in the expression of genes for specific TFs,cytokines,ECM proteins and matrix proteinases,as well the significance of these epigenetic mechanisms in the pathogenesis of OA.

关键词： Epigenetics Osteoarthritis Transcription factor Cytokine Matrix proteinase

Brassinosteroids modulate nitrogen physiological response and promote nitrogen uptake in maize(Zea mays L.)

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The Crop Journal 2022年第1期10卷 166-176页

作者： Jiapeng Xing Yubin Wang Qingqing Yao Yushi zhang mingcai zhang Zhaohu Li State Key Laboratory of Plant Physiology and Biochemistry College of Agronomy and BiotechnologyChina Agricultural UniversityBeijing 100193China Crop Research Institute Shandong Academy of Agricultural SciencesJinan 250100ShandongChina

Brassinosteroids(BRs)are steroid hormones that function in plant growth and development and response to environmental stresses and nutrient ***,few studies have investigated the effect of BRs in modulating the physiological response to nitrogen(N)supply in *** the present study,BR signalingdeficient mutant zmbri1-RNAi lines and exogenous application of 2,4-epibrassinolide(e BL)were used to study the role of BRs in the regulation of physiological response in maize seedlings supplied with *** application of e BL increased primary root length and plant biomass,but zmbri1 plants showed shorter primary roots and less plant biomass than wild-type plants under low N(LN)and normal N(NN)*** induced the expression of the BR signaling-associated genes Zm DWF4,Zm CPD,Zm DET2,and Zm BZR1 and the production of longer primary roots than *** of Zm BRI1 weakened the biological effects of LN-induced primary root elongation.e BL treatment increased N accumulation in shoots and roots of maize seedlings exposed to LN or NN ***,zmbri1 plants showed lower N accumulation in shoots and roots than wild-type *** with reduced N accumulation,zmbri1 plants showed lower NO3-fluxes and^(15)NO_(3)^(-)*** expression of nitrate transporter(NRT)genes(Zm NPF6.4,Zm NPF6.6,Zm NRT2.1,Zm NRT2.2)was lower in zmbri1 than in wild-type roots,but e BL treatments up-regulated the transcript expression of NRT ***,BRs modulated N physiological response and regulated the transcript expression of NRT genes to promote N uptake in maize.

关键词： Brassinosteroids Nitrogen uptake Nitrate transporter gene Root architecture Maize

Exogenous application of glycine betaine improved water use efficiency in winter wheat(Triticum aestivum L.) via modulating photosynthetic efficiency and antioxidative capacity under conventional and limited irrigation conditions

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The Crop Journal 2019年第5期7卷 635-650页

作者： Nazir Ahmed Yushi zhang Ke Li Yuyi Zhou mingcai zhang Zhaohu Li Engineering Research Center of Plant Growth Regulator Ministry of Education College of Agronomy and Biotechnology China Agricultural University

Improving water use efficiency(WUE)is an important subject in agricultural irrigation for alleviating the scarcity of water resources in semiarid regions of the North China ***,glycine betaine(GB)is one of the most effective compatible solutes synthesized naturally in plants for enhancing stress tolerance under abiotic stress,but little information is available on the involvement of GB in regulating crop WUE under field *** study was conducted to explore the role of exogenously applied GB in improving WUE and plant physiological and biochemical responses inwinterwheat subjected to conventional or limited irrigation during the 2015–2016 and 2016–2017 growing *** application of GB significantly enhanced antioxidant enzyme activities and reduced the accumulation ofmalondialdehyde and hydrogen peroxide under limited irrigation ***,GB-treated plantsmaintained higher leaf relative water content andmembrane stability,which led to higher chlorophyll content and gas exchange attributes for better intrinsic and instantaneouswater use efficiencies compared to control plants under limited irrigation ***-treated plants had higher indole-acetic acid and zeatin riboside levels but lower ABA levels compared to control plants under conventional and limited irrigation ***,GB enhanced the grain filling rate and duration,grain number per spike,and final grainweight,which resulted in higher grain yield compared to the ***,GB significantly improved the integrative and photosynthetic WUE under conventional and limited irrigation conditions,although GB treatment did not markedly affect total water *** results suggest the involvement of GB in improving WUEs in winter wheat by modulating hormonal balance,membrane stability,photosynthetic performance and antioxidant systems to maintain higher grain yield under conventional and limited irrigation conditions.

关键词： Winter wheat Glycine betaine Water use efficiency Limited irrigation Photosynthetic performance Antioxidant systems

Ethylene accelerates maize leaf senescence in response to nitrogen deficiency by regulating chlorophyll metabolism and autophagy

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The Crop Journal 2024年第5期12卷 1391-1403页

作者： Jiapeng Xing Ying Feng Yushi zhang Yubin Wang Zhaohu Li mingcai zhang State Key Laboratory of Plant Environmental Resilience College of Agronomy and BiotechnologyChina Agricultural UniversityBeijing 100193China College of Agriculture Northeast Agricultural UniversityHarbin 150030HeilongjiangChina

Leaf senescence is an orderly and highly coordinated process,and finely regulated by ethylene and nitrogen(N),ultimately affecting grain yield and nitrogen-use efficiency(NUE).However,the underlying regulatory mechanisms on the crosstalk between ethylene-and N-regulated leaf senescence remain a mystery in *** this study,ethylene biosynthesis gene ZmACS7 overexpressing(OE-ZmACS7)plants were used to study the role of ethylene regulating leaf senescence in response to N deficiency,and they exhibited the premature leaf senescence accompanied by increased ethylene release,decreased chlorophyll content and F_v/F_m ratio,and accelerated chloroplast ***,we investigated the dynamics changes of transcriptome reprogramming underlying ethylene-accelerated leaf senescence in response to N *** differentially expressed genes(DEGs)involved in chlorophyll biosynthesis were significantly down-regulated,while DEGs involved in chlorophyll degradation and autophagy processes were significantly up-regulated,especially in OE-ZmACS7 plants in response to N deficiency.A gene regulatory network(GRN)was predicted during ethylene-accelerated leaf senescence in response to N *** transcription factors(TFs)ZmHSF4,Zmb HLH106,and ZmEREB147 were identified as the key regulatory genes,which targeted chlorophyll biosynthesis gene ZmLES22,chlorophyll degradation gene ZmNYC1,and autophagy-related gene ZmATG5,***,ethylene signaling key genes might be located upstream of these TFs,generating the signaling cascade networks during ethylene-accelerated leaf senescence in response to N ***,these findings improve our molecular knowledge of ethylene-accelerated maize leaf senescence in response to N deficiency,which is promising to improve NUE by manipulating the progress of leaf senescence in maize.

关键词： Ethylene Leaf senescence N deficiency Chlorophyll metabolism Autophagy Gene regulatory network

GhKT2: a novel K^+ transporter gene in cotton(Gossypium hirsutum)

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Frontiers of Agricultural Science and Engineering 2018年第2期5卷 226-235页

作者： Yiru WANG Juan XU mingcai zhang Xiaoli TIAN Zhaohu LI Department of Agronomy College of Agronomy and Biotechnology China Agricultural University

Potassium is an essential nutrient for plant growth and productivity of crops. K+transporters are important for K+uptake and transport in plants. However,information on the function of K+transporters and K+channels in cotton is limited. The KT/KUP/HAK protein family is essential for a variety of physiological processes in plants, including nutrient acquisition and regulation of development. This study, identified a K+transporter gene,Gh KT2, expressed in the roots of cotton(Gossypium hirsutum) cv. Liaomian17. The deduced transcript of Gh KT2 is highly homologous to Cluster II of KUP/HAK/KT K+transporters and is predicted to contain 11 transmembrane domains. Gh KT2 has been localized to the plasma membrane, and its transcripts were detected in roots, stems, leaves and shoot apices of cotton ***, b-glucuronidase(GUS) expression driven by the Gh KT2 promoter could be detected in roots, mesophyll cells, and leaf veins in transgenic Arabidopsis. In addition,the expression of Gh KT2 was induced by low K+stress in cotton roots and p Gh KT2::GUS-transgenic Arabidopsis seedlings. The Gh KT2-overexpression Arabidopsis lines plants were larger and showed greater K+accumulation than the wild type(WT) regardless of K+concentration supplied. The net K+influx rate, measured by the noninvasive micro-test technique, in root meristem zone of Gh KT2-transgenic Arabidopsis lines was significantly greater than that of WT. Taken together, this evidence indicates that Gh KT2 may participate in K+acquisition from low or high external K+, as well as K+transport and distribution in plants.

关键词： cotton GhKT2 potassium transporter uptake

Increase in root density induced by coronatine improves maize drought resistance in North China

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The Crop Journal 2023年第1期11卷 278-290页

作者： Yuling Guo Guanmin Huang Qing Guo Chuanxi Peng Yingru Liu mingcai zhang Zhaohu Li Yuyi Zhou Liusheng Duan State Key Laboratory of Plant Physiology and Biochemistry College of Agronomy and BiotechnologyChina Agricultural UniversityBeijing 100193China

Drought stress caused by insufficient irrigation or precipitation impairs agricultural production *** this study,a two-year field experiment was conducted to investigate the effect of coronatine(COR),a functional analog of jasmonic acid(JA),on maize drought *** experiment included two water treatments(rainfed and irrigation),four COR concentrations(mock,0μmol L^(-1);A1,0.1μmol L^(-1);A2,1μmol L^(-1);A3,10μmol L^(-1))and two maize genotypes(Fumin 985(FM985),a drought-resistant cultivar and Xianyu 335(XY335),a drought-sensitive cultivar).Spraying 1μmol L^(-1)COR at seedling stage increased surface root density and size,including root dry matter by 12.6%,projected root area by 19.0%,average root density by 51.9%,and thus root bleeding sap by 28.2%under drought *** application also increased leaf area and SPAD values,a result attributed to improvement of the root system and increases in abscisic acid(ABA),JA,and salicylic acid(SA)*** improvement of leaves and roots laid the foundation for increasing plant height and dry matter *** application reduced anthesis and silking interval,increasing kernel number per *** treatment at 1μmol L^(-1)increased the yield of XY335 and FM985 by 7.9%and 11.0%,*** and path analysis showed that grain yields were correlated with root dry weight and projected root area,increasing maize drought resistance mainly via leaf area index and dry matter ***,COR increased maize drought resistance mainly by increasing root dry weight and root area,with 1μmol L-^(-1)COR as an optimal concentration.

关键词： Maize Drought resistance Coronatine Root development Grain yields

Coronatine promotes maize water uptake by directly binding to the aquaporin ZmPIP2;5 and enhancing its activity

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Journal of Integrative Plant Biology 2023年第3期65卷 703-720页

作者： Rui He Huiqing Su Xing Wang Zhijie Ren Kun zhang Tianyu Feng mingcai zhang Zhaohu Li Legong Li Junhong Zhuang Zhizhong Gong Yuyi Zhou Liusheng Duan State Key Laboratory of Plant Physiology and Biochemistry Engineering Research Center of Plant Growth RegulatorMinistry of Education&College of Agronomy and BiotechnologyChina Agricultural UniversityBeijing 100193China College of Plant Protection China Agricultural UniversityBeiing 100193China Key Laboratory of Plant Gene Resources and Biotechnology for Carbon Reduction and Environmental Improvement Beijing Municipal GovernmentCollege of Life SciencesCapital Normal UniversityBeijing 100048China Center for Crop Functional Genomics and Molecular Breeding China Agricultural UniversityBeijing 100193China State Key Laboratory of Plant Physiology and Biochemistry College of Biological SciencesChina Agricultural UniversityBeijing 100193China

Water uptake is crucial for crop growth and development and drought stress tolerance. The water channel aquaporins(AQP) play important roles in plant water uptake. Here, we discovered that a jasmonic acid analog, coronatine(COR), enhanced maize(Zea mays) root water uptake capacity under artificial water deficiency conditions. COR treatment induced the expression of the AQP gene Plasma membrane intrinsic protein 2;5(ZmPIP2;5).In vivo and in vitro experiments indicated that COR also directly acts on ZmPIP2;5 to improve water uptake in maize and Xenopus oocytes. The leaf water potential and hydraulic conductivity of roots growing under hyperosmotic conditions were higher in ZmPIP2;5-overexpression lines and lower in the zmpip2;5 knockout mutant, compared to wild-type plants. Based on a comparison between ZmPIP2;5 and other PIP2s, we predicted that COR may bind to the functional site in loop E of ZmPIP2;5. We confirmed this prediction by surface plasmon resonance technology and a microscale thermophoresis assay, and showed that deleting the binding motif greatly reduced COR binding. We identified the N241 residue as the COR-specific binding site, which may activate the channel of the AQP tetramer and increase water transport activity,which may facilitate water uptake under hyperosmotic stress.

关键词： coronatine osmotic stress water uptake capacity ZmPIP2 5

Analysis of differential expression of genes induced by ethephon in elongating internodes of maize plants

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Frontiers of Agricultural Science and Engineering 2016年第3期3卷 263-282页

作者： Xiaoyi WEI Weiqiang zhang Qian zhang Pei SUN Zhaohu LI mingcai zhang Jianmin LI Liusheng DUAN State Key Laboratory of Plant Physiology and Biochemistry Engineering Research Center of Plant Growth Regulator Ministry of Education/College of Agronomy China Agricultural University Xinxiang Academy of Agricultural Science

Plant growth regulators(PGRs) are commonly used in cereal cropping systems to restrict plant height and control lodging. Ethephon has been reported to shorten internodes and increase grain yield of maize. To analyze the transcriptomic profiles of maize internode elongation following ethephon treatment, differentially expressed genes were compared between the treatment and contro samples of inbred line Zong 31 using the Affymetrix Maize Genome Array. According to the microarray data 326 probe sets showed significant change in expression Further research revealed that the most remarkable effects of ethephon on maize internodes elongation occurred during a 48 h period, when 89 differentially expressed genes were detected. There were dramatic change in transcript levels at 24 h and six Auxin transport genes and four gibberellin biosynthesis pathway genes were differentially expressed in Zong 31 in response to ethephon treatment. In summary, we showed that gaseous ethylene release is involved in internode meristem cell elongation through the regulation of plant hormone signaling in maize. This work provides a platform for studies in which candidate genes will be functionally tested for involvement in internode elongation.

关键词： maize ethephon internode elongation microarray qPCR