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Indirect plant defense against insect herbivores： a review

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Insect Science 2018年第1期25卷 2-23页

作者： Zainab Aljbory ming-shun chen Department of Entomology Kansas State University Manhattan Kansas USA Hard Winter Wheat Genetics Research Unit USDA-ARS Kansas State University Manhattan Kansas USA

Plants respond to herbivore attack by launching 2 types of defenses： direct defense and indirect defense. Direct defense includes all plant traits that increase the resistance of host plants to insect herbivores by affecting the physiology and/or be- havior of the attackers. Indirect defense includes all traits that by themselves do not have significant direct impact on the attacking herbivores, but can attract natural en- emies of the herbivores and thus reduce plant loss. When plants recognize herbivore- associated elicitors, they produce and release a blend of volatiles that can attract preda- tors, parasites, and other natural enemies. Known herbivore-associated elicitors include fatty acid-amino acid conjugates, sulfur-containing fatty acids, fragments of cell walls, peptides, esters, and enzymes. Identified plant volatiles include terpenes, nitrogenous compounds, and indoles. In addition, constitive traits including extrafloral nectars, food bodies, and domatia can be further induced to higher levels and attract natural enemies as well as provide food and shelter to carnivores. A better understanding of indirect plant defense at global and componential levels via advanced high throughput technolo- gies may lead to utilization of indirect defense in suppression of herbivore damage to plants.

关键词： elicitor indirect plant defense natural enemies volatile

Identification of a major QTL for Hessian fly resistance in wheat cultivar‘Chokwang’

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The Crop Journal 2022年第3期10卷 775-782页

作者： Lirong Zhang Yunfeng Xu ming-shun chen Zhenqi Su Yang Liu Yuzhou Xu Guixiao La Guihua Bai Department of Plant Pathology College of Plant ProtectionHebei Agricultural UniversityBaoding 071001HebeiChina Department of Agronomy Kansas State UniversityManhattanKS 66506USA Technological Innovation Center for Biological Control of Crop Diseases and Insect Pests of Hebei Province Baoding 071001HebeiChina Hard Winter Wheat Genetics Research Unit USDA-ARSManhattanKS 66506USA State Key Laboratory for Agrobiotechnology and Key Laboratory of Crop Heterosis and Utilization(MOE)and Beijing Key Laboratory of Crop Genetic Improvement China Agricultural UniversityBeijing 100193China Institute of South Subtropical Crops Chinese Academy of Tropical Agricultural SciencesZhanjiang 524013GuangdongChina Industrial Crop Research Institute Henan Academy of Agricultural SciencesZhengzhou 450002HenanChina

The Hessian fly(HF,Mayetiola destructor)is one of the destructive pests of wheat(Triticum aestivum L.)*** cultivars can effectively minimize wheat damage due to this insect *** identify new quantitative trait loci(QTL)for HF resistance,a population of recombinant inbred lines(RILs)was developed from a cross between the HF-resistant wheat cultivar‘Chokwang’and susceptible wheat‘Ning 7840’,and phenotyped for responses to HF attack.A linkage map was constructed using 1147 single nucleotide polymorphism(SNP)markers generated from genotyping-by-sequencing(GBS).One major QTL,***-6 BS,for HF-resistance was identified on chromosome arm 6 BS,which explained up to84.0%of the phenotypic variation and was contributed by *** RILs showed recombination in the candidate interval of ***-6 BS,which delimited ***-6 BS to a 4.75 Mb physical interval between 6,028,601 bp and 10,779,424 bp on chromosome arm 6 BS of IWGSC Chinese Spring reference genome Ref Seq *** GBS-SNPs in the candidate interval were converted into Kompetitive allele specific polymerase chain reaction(KASP)*** of them,KASP-6 B112698 and KASP-6 B7901215,were validated in a *** wheat ***-6 B112698 was nearly diagnostic,thus can be used to screen ***-6 BS and pyramid it with other resistance genes in breeding programs.

关键词： Wheat Hessian fly resistance Mayetiola destructor KASP GBS

Genes encoding a group of related small secreted proteins from the gut of Hessian fly larvae [Mayetiola destructor （Say）]

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Insect Science 2006年第5期13卷 339-348页

作者： ming-shun chen XIANG LIU YU-chenG ZHU JOHN C. REESE GERALD E. W ILDE USDA-ARS Plant Science and Entomology Research UnitUSA Department of Entomology Kansas State University Manhattan KansasUSA USDA-ARS-JWDSRC Stoneville Minnesota USA

A group of related genes has been isolated and characterized from the gut of Hessian fly larvae [Mayetiola destructor （Say）]. Members in this group appear to encode proteins with secretary signal peptides at the N-terminals. The mature putative proteins are small, acidic proteins with calculated molecular masses of 14.5 to 15.3 kDa, and isoelectric points from 4.56 to 4.88. Northern blot analysis revealed that these genes are expressed predominantly in the gut of Hessian fly larvae and pupae. Two related genes, GIOK1 and GIOK2, were isolated as tandem repeats. Both genes contain three exons and two introns. The intron/exon boundaries were conserved in terms of amino acid encoding, suggesting that they arose by gene duplication. The fact that the frequency of this group of clones in a gut cDNA library higher than that of total cDNA clones encoding digestive enzymes suggested that this group of proteins may perform an important function in the gut physiology of this insect. However, the exact functions of these proteins are as yet known since no sequence similarity could be identified between these proteins and any known sequences in public databases using standard methods.

关键词： Hessian fly gut Mayetiola destructor secreted protein wheat

逆向工程中基于三目视觉自动提取并构造复杂曲面边界技术

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机器人 2002年第1期24卷 66-70,85页

作者：何炳蔚林志航杨明顺陈琨西安交通大学CIMS中心 710049

在逆向工程中复杂曲面零件表面的几何模型的构造是研究重点之一，根据零件表面的数字化数据提取零件表面的边界是构造零件几何模型的关键步骤．本文提出基于三目视觉方法来提取和构造复杂曲面边界的技术．从一幅图像中提取反映物体边... 详细信息

在逆向工程中复杂曲面零件表面的几何模型的构造是研究重点之一，根据零件表面的数字化数据提取零件表面的边界是构造零件几何模型的关键步骤．本文提出基于三目视觉方法来提取和构造复杂曲面边界的技术．从一幅图像中提取反映物体边界的特征点，再利用图像匹配方法得到这些特征点的空间坐标，最后以这些特征点构造出物体边界Ｂ样条曲线．

关键词：逆向工程计算机辅助设计复杂曲面边界几何模型三目视觉自动提取

LC-MS法评价2种盐酸非索非那定制剂的人体生物等效性

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沈阳药科大学学报 2011年第4期28卷 287-290,324页

作者：闫凯张明毕开顺陈晓辉沈阳药科大学药学院辽宁沈阳110016

目的建立LC-MS法测定人血浆中非索非那定浓度,评价盐酸非索非那定胶囊（受试制剂）与盐酸非索非那定片（参比制剂）的人体生物等效性。方法采用Kromasil C18（150 mm×4.6 mm,5μm）色谱柱;流动相：甲醇-质量分数为1%的甲酸水溶液（体... 详细信息

目的建立LC-MS法测定人血浆中非索非那定浓度,评价盐酸非索非那定胶囊（受试制剂）与盐酸非索非那定片（参比制剂）的人体生物等效性。方法采用Kromasil C18（150 mm×4.6 mm,5μm）色谱柱;流动相：甲醇-质量分数为1%的甲酸水溶液（体积比为88∶12）;离子源：大气压化学电离源,扫描方式：选择离子监测,用于定量分析的离子分别为m/z502.15（非索非那定）和m/z383.00（内标氯雷他定）。本法用于健康受试者口服盐酸非索非那定受试制剂和参比制剂的生物等效性研究。结果非索非那定质量浓度在10.0-2000.0μg.L-1内线性关系良好,方法的定量下限（LLOQ）为10.0μg.L-1。受试制剂与参比制剂的主要药物动力学参数：tmax分别为（1.9±0.2）和（1.9±0.2）h;ρmax分别为（173±77）和（183±83）μg.L-1;t1/2分别为（5.6±2.6）和（5.8±2.4）h;AUC0-t分别为（982±583）和（1081±772）μg.h.L-1,AUC0-∞分别为（1 176±738）and（1 271±998）μg.h.L-1。盐酸非索非那定胶囊的相对生物利用度为（90.8±15.3）%（n=18）。结论 2种盐酸非索非那定制剂具有生物等效性。

关键词：盐酸非索非那定药物动力学生物等效性液相色谱-质谱法

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基于公共网络的远程温度监测系统的设计

电气电子教学学报 2016年第4期38卷 82-85页

作者：路明吉顺平陈秋阳三江学院电气学院

温度信息由单片机处理并上传给上位机采集系统。在上位机中,使用VB编写程序,将处理好的数据传送到公共网络上的数据库中。使用ASP软件开发了动态网页,网页由FTP上传到公共服务器中。各种数字终端(计算机、手机和IPAD等)都可以浏览网页,... 详细信息

温度信息由单片机处理并上传给上位机采集系统。在上位机中,使用VB编写程序,将处理好的数据传送到公共网络上的数据库中。使用ASP软件开发了动态网页,网页由FTP上传到公共服务器中。各种数字终端(计算机、手机和IPAD等)都可以浏览网页,实现设备的温度远程监测。

关键词：公共网络数据库动态网页

Optimization of multi-model ensemble forecasting of typhoon waves

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Water Science and Engineering 2016年第1期9卷 52-57页

作者： shun-qi Pan Yang-ming Fan Jia-ming chen Chia-chuen Kao Hydro-environmental Research Centre School of Engineering Cardiff University Coastal Ocean Monitoring Center National Cheng Kung University Department of Hydraulic and Ocean Engineering National Cheng Kung University

Accurately forecasting ocean waves during typhoon events is extremely important in aiding the mitigation and minimization of their potential damage to the coastal infrastructure, and the protection of coastal communities. However, due to the complex hydrological and meteorological interaction and uncertainties arising from different modeling systems, quantifying the uncertainties and improving the forecasting accuracy of modeled typhoon-induced waves remain challenging. This paper presents a practical approach to optimizing model-ensemble wave heights in an attempt to improve the accuracy of real-time typhoon wave forecasting. A locally weighted learning algorithm is used to obtain the weights for the wave heights computed by the WAVEWATCH III wave model driven by winds from four different weather models （model-ensembles）. The optimized weights are subsequently used to calculate the resulting wave heights from the model-ensembles. The results show that the opti- mization is capable of capturing the different behavioral effects of the different weather models on wave generation. Comparison with the measurements at the selected wave buoy locations shows that the optimized weights, obtained through a training process, can significantly improve the accuracy of the forecasted wave heights over the standard mean values, particularly for typhoon-induced peak waves. The results also indicate that the algorithm is easy to imnlement and practieal for real-time wave forecasting.

关键词： Wave modeling Optimization Forecasting Typhoon waves WAVEWATCH III Locally weighted learning algorithm

Detection of apoptosis induced by new type gosling viral enteritis virus in vitro through fluorescein annexin V-FITC/PI double labeling

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World Journal of Gastroenterology 2008年第14期14卷 2174-2178页

作者： shun chen An-Chun cheng ming-Shu Wang Xi Peng Avian Diseases Research Centre College of Veterinary Medicine of Sichuan Agricultural University Yaan 625014 Sichuan Province China Avian Diseases Research Centre College of Veterinary Medicine of Sichuan Agricultural University Key Laboratory of Animal Diseases and Human Health of Sichuan Province Yaan 625014 Sichuan Province China College of Life Science and Technology of Southwest University for Nationalities Chengdu 610041 Sichuan Province China

AIM: To achieve a better understanding of the pathogenesis of new type gosling viral enteritis virus (NGVEV) and the relationship between NGVEV and host cells. METHODS: The apoptosis of duck embryo fibroblasts (DEF) induced by NGVEV was investigated by fluorescence-activated cell sorter (FACS) and fluorescence microscope after the cells were stained with Annexin V-FITC and propidium iodide (PI). RESULTS: By staining cells with a combination of fluorescein annexin V-FITC and PI, it is possible to distinguish and quantitatively analyze non-apoptotic cells (Annexin V-FITC negative/PI negative), early apoptotic cells (Annexin V-FITC positive/PI negative), late apoptotic/necrotic cells (Annexin V-FITC positive/ PI positive) and dead cells (Annexin V-FITC negative/PI positive) through flow cytometry and fluorescence microscope. The percentage of apoptotic cells increased with the incubation time and reached a maximum at 120 h after infection, while the percentage of non- apoptotic cells decreased.

关键词： Gosling viral enteritis New type Virus Duck embryo fibroblasts Apoptosis Fluorescein annexin V-FITC/PI

Development of Species-Specific PCR Primers and Sensitive Detection of the Tylenchulus semipenetrans in China

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Agricultural Sciences in China 2011年第2期10卷 252-258页

作者： LIU Guo-kun chen Juan XIAO shun ZHANG Shao-sheng PAN Dong-ming College of Plant Protection Fujian Agriculture and Forestry UniversityFuzhou 350002P.R.China College of Horticulture Fujian Agriculture and Forestry UniversityFuzhou 350002P.R.China

Tylenchulus semipenetrans is the most economically important and widespread nematode pest of citrus in ***-ITS of 14 populations of *** which were collected from different citrus groves or Chinese fir（Cunninghamia lanceolata） plantations in China were amplified and *** species-specific primers were designed for the first time to diagnosis *** based on the sequences of rDNA-ITS regions of geographic population *** primers were sensitive to amplify the expected band size（297 bp） from DNA template of a single second-stage juvenile（J2） or different life stages of *** specific band was amplified from 15 non-target nematode species which were commonly found in citrus *** and reliability of the primers were validated by further PCR amplification of 16 extra populations of *** collected from 4 provinces of *** primers successfully detected a single J2 of *** within a whole nematode community comprising a large numbers of non-target *** developed diagnostic technique can be used for accurate identification of *** and also as a decision tool for nematode management for citrus or Chinese fir in China.

关键词： Tylenchulus semipenetrans rDNA-ITS species-specific primers diagnosis detection