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REAL-TIME MONITORING OF MITOCHONDRIAL FUNCTION AND CEREBRAL BLOOD FLOW FOLLOWING FOCAL ISCHEMIA IN RATS

Journal of Innovative Optical Health Sciences 2008年第1期1卷 63-69页

作者： AMIR LIVNAT michael tolmasov EFRAT BARBIRO-michaelY AVRAHAM MAYEVSKY The Mina&Everard Goodman Faculty of Life-Sciences and The Gonda Multidisciplinary Brain Research Center Bar-Ilan University Ramat-Gan 52900Israel

Focal ischemia due to reduction of cerebral blood flow(CBF),creates 2 zones of damage:the core area,which suffers severe damage,and penumbra area,which surrounds the core and suffers intermediate levels of injury.Objectives:A novel method is introduced,which evaluates mitochondrial function in the core and in the penumbra,during focal cerebral ischemia.Methods:Wistar rats underwent focal cerebral ischemia by middle cerebral artery occlusion(MCAO)for 60 minutes,followed by 60 minutes of reperfusion.Mitochondrial function was assessed by a unique Multi-Site—Multi-Parametric(MSMP)monitoring system,which measures mitochondrial NADH using fluorometric technique,and CBF using Laser Doppler Flowmetry(LDF).Results:At the onset of occlusion,CBF dropped and NADH increased significantly only in the right hemisphere.CBF levels were significantly lower and NADH significantly higher in the core than in the penumbra.After reperfusion,CBF and NADH recovered correspondingly to the intensity of ischemia.Conclusion:Application of the MSMP system can add significant information for the understanding of the cerebral metabolic state under ischemic conditions,with an emphasis on mitochondrial function.

关键词： Mitochondrial function cerebral blood flow focal ischemia

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EFFECTS OF SEVERE HEMORRHAGE ON IN VIVO BRAIN AND SMALL INTESTINE MITOCHONDRIAL NADH AND MICROCIRCULATORY BLOOD FLOW

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Journal of Innovative Optical Health Sciences 2008年第2期1卷 177-183页

作者： MIRA M.MANDELBAUM EFRAT BARBIRO-michaelY michael tolmasov AVRAHAM MAYEVSKY The Mina&Everard Goodman Faculty of Life-Sciences and The Gonda Multidisciplinary Brain Research Center Bar-Ilan University Ramat-Gan52900Israel

Severe body stress induced by hypoxemia and hypotension may lead to total body energy state deterioration.The perfusion of the most vital organs is maintained at the expense of“less vital”organs.In the present study,we used a multi-site multiparametric(MSMP)monitoring system for real-time evaluation of tissue blood flow(TBF)and mitochondrial NADH fluorescence of the brain and the small intestine following hemorrhage.In Group 1,uncontrolled hemorrhage,mean arterial pressure(MAP)was decreased to 40mmHg within 2 minutes and shed blood was re-infused after 30minutes.In Group 2,controlled hemorrhage,during the 30minutes of hemorrhage,MAP was kept at 40mmHg.During hemorrhage,in both groups,the intestinal TBF and NADH deteriorated,while the brain remained relatively well protected.In Group 1,all parameters partly recovered within the hemorrhage phase,while in Group 2,complete recovery occurred only after resuscitation.At the end of the experiment,both models showed a decrease in intestinal viability(TBF decreased,NADH increased),while the brain metabolic state in Group 2 declined slightly.Our unique multi-parametric monitoring device demonstrated that,under hemorrhage,the small intestine responded entirely differently from the brain.This may suggest the potential usefulness of the monitoring of less vital organs,as proxy organs,in critical conditions such as massive hemorrhage.The present study also highlights the importance of mitochondrial function monitoring in similar conditions in the clinical environment.

关键词： Mitochondrial dysfunction multiparametric monitoring Laser Doppler Flowmetery fluorometric NADH monitoring

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