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Fabrication and Tissue Anchoring Performance of Nylon and Polypropylene Barbed Surgical Sutures

Journal of Donghua University(English Edition) 2013年第5期30卷 391-393页

作者：丛慧 ROE Simon mente peter RUFF Gregory KING W Martin College of Textiles North Carolina State University College of Veterinary Medicine North Carolina State University Department of Biomedical Engineering North Carolina State University Vilcom Circle Chapel Hill College of Textiles Donghua University

Barbed surgical sutures have attracted worldwide attention in recent years. The plurality of barbs projecting out and penetrating into surrounding tissue eliminates the need for tying a knot. In the current study,nylon and polypropylene( PP) barbed sutures with two different barb geometries designed to anchor in porcine skin and tendon tissues were fabricated by a specially designed mechanical cutting machine. The tensile properties were compared between the barbed and unbarbed control sutures. The in vitro tissue anchoring ability was measured by two tissue /suture pullout tests,one for skin and the other for tendon tissue. Regardless of the type of tissue,the PP barbed suture with its higher modulus had a superior anchoring ability. Given the same suture material,improved anchoring performance was shown in tendon tissue.

关键词： barbed surgical sutures nylon polypropylene(PP) barb configuration skin and tendon

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Identification of stable normalization genes for quantitative real-time PCR in porcine articular cartilage

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Journal of Animal Science and Biotechnology 2012年第4期3卷 181-187页

作者： Ryan S McCulloch Melissa S Ashwell Audrey T O'Nan peter L mente Joint Department of Biomedical Engineering North Carolina State University University of North Carolina Animal Science Department North Carolina State University

Background： Expression levels for genes of interest must be normalized with an appropriate reference, or housekeeping gene, to make accurate comparisons of quantitative real-time PCR results. The purpose of this study was to identify the most stable housekeeping genes in porcine articular cartilage subjected to a mechanical injury from a panel of 10 candidate genes. Results： Ten candidate housekeeping genes were evaluated in three different treatment groups of mechanically impacted porcine articular cartilage. The genes evaluated were： beta actin, beta-2-microglobulin, glyceraldehyde-3-phosphate dehydrogenase, hydroxymethylbilane synthase, hypoxanthine phosphoribosyl transferase, peptidylprolyl isomerase A （cyclophilin A）, ribosomal protein L4, succinate dehydrogenase flavoprotein subunit A, TATA box binding protein, and tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein--zeta polypeptide The stability of the genes was measured using geNorm, BestKeeper, and NormFinder software. The four most stable genes measured via geNorm were （most to least stable） succinate dehydrogenase flavoprotein, subunit A, peptidylprolyl isomerase A, glyceraldehyde-3-phosphate dehydrogenase, beta actin; the four most stable genes measured via BestKeeper were glyceraldehyde-3-phosphate dehydrogenase, peptidylprolyl isomerase A, beta actin, succinate dehydrogenase flavoprotein, subunit A; and the four most stable genes measured via NormFinder were peptidylprolyl isomerase A, sucdnate dehydrogenase flavoprotein, subunit A, glyceraldehyde-3-phosphate dehydrogenase, beta actin. Conclusions： BestKeeper, geNorm, and NormFinder all generated similar results for the most stable genes in porcine articular cartilage. The use of these appropriate reference genes will facilitate accurate gene expression studies of porcine articular cartilage and suggest appropriate housekeeping genes for articular cartilage studies in other species.

关键词： Cartilage Housekeeping Normalization Porcine Reference Stability

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