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Chemical Research in Chinese Universities 2001年第1期17卷 121-126页

作者： meng xiang-ying LI xiang-gao TIAN Yi-xin LIU Yin-yan ZHENG Lian-xiu College of Chinese Medical Material Jilin Agricultural UniversityChangchun 130118P.R.China The School of Basic Medical Sciences Norman Bethune University of Medical SciencesChangchun 130021P.R.China People Hospital of Jilin Province Changchun 130021P.R.China

Chemical constituents namely flavonoids in the flower buds of Panax quinque folium were identified and their content was determined in different parts. The content determination was performed using dual wavelength TLC-scan and ultraviolet absorption. Content determination results showed that the content of flavonoid in the flower bud was higher than in the roots.

关键词： Panax quinquefulium Flower buds Flavonoid Root Fruit

Construction and Characterization of a cDNA Expression Library for the North American Ginseng Root Tissues

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Chemical Research in Chinese Universities 2007年第3期23卷 310-313页

作者： WANG ying WANG Kun BAO Yong-li WU Yin meng xiang-ying LI Yu-xin Research Center of Agriculture and Medicine Gene Engineering of Ministry of Education Institute of Genetics and Cytology Northeast Normal University Changchun 130024 P. R. China

The root of Panax ginseng plant undergoes a specific developmental process to become a biosynthesis and accumulation tissue for ginsenosides. To identify and analyze genes involved in the biosynthesis of ginsenoside, we constructed and characterized a full-length cDNA library for 6-year-old North American ginseng. The titer of primary cDNA library is 1.2 × 10^6 pfu/mL, the titer of amplified library is 2. 6 × 10^10 pfu/mL and the rate of recombinant is above 86%. The insert size ranges from 0. 3 to 2.0 kb. Sequencing results show that 18 of 58 genes are high homologous to the genes （GBRS, GBR3 and GBR1 ） known in GenBank, which are involved in biosynthesis of ginsenoside in North American ginseng plant; 16 of 58 genes are novel genes. The full-length cDNA library of North American ginseng root tissues is essential for the cloning of genes known and it is also an initial key for the screening and cloning of new genes.

关键词： cDNA library North American ginseng SMART

Mutation Analysis of AVPR2 and AQP2 Gene in Chinese Patients with Congenital Nephrogenic Diabetes Insipidus

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Chemical Research in Chinese Universities 2008年第3期24卷 312-315页

作者： WANG ying LI Hong-jun YU Zhen-xiang BAO Yong-li WU Yin YU Chun-lei meng xiang-ying LI Yu-xin Institute of Genetics and Cytology Northeast Normal University Changchun 130024 P. R. China Out-patient Department China-Japan Union Hospital of Jilin University Changchun 130033 P. R. China Department of Respiratory Medicine First Hospital of Jilin University Changchun 130021 P. R. China Research Center of Agriculture and Medicine Gene Engineering of Ministry of Education Northeast Normal University Changchun 130024 P. R. China

To detect mutations of the aquaporin 2 gene（AQP2） and the arginine vasopressin V2 receptor gene（AVPR2） of Chinese congenital nephrogenic diabetes insipidus, and to establish the foundation for further studying the emergence mechanism of the disease and clinical diagnosis, all the exons and part of introns of AQP2 and AVPR2 genes were amplified with intronic primers, using genomic DNA extracted from three patients with congenital nephrogenic diabetes insipidus and two mothers as template, PCR product was ligated into a T-vector and then sequenced. The result was compared with the database sequence to identify the mutable sites via a BLAST search, the incidence of every mutation was analyzed, and the putative transcription factor binding sites that maybe disturbed were analyzed by MAPPER. Mutation g.1394A〉G in exon 3 of AVPR2 was detected in all the subjects, g.861C〉T（S167L） in exon 2 of AVPR2 and IVS1＋3G〉A in intron of AQP2 were detected, respectively, in two patients, and c.836A〉C in 3′ untranslated region of AQP2 was detected in two patients and one mother. Four mutations were identified. g.1394A〉G of AVPR2 and c.836A〉C of AQP2 have high incidence in patients with nephrogenic diabetes insipidus. Detection on the two sites may become auxiliary diagnosis index of congenital nephrogenic diabetes insipidus.

关键词： Nephrogenic diabetes insipidus Gene mutation AQP2 A VPR2

Components of Essential Oils in Different Parts of Daucus carota L. var. sativa Hoffm

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Chemical Research in Chinese Universities 2006年第3期22卷 328-334页

作者： WU Yin XU Ze-Li LI Hong-jun meng xiang-ying BAO Yong-li LI Yu-xin Research Center of Agriculture and Medicine C ene Engineering of Ministry of Education Institute of Genetics and Cytology Northeast Normal University Changchun 130024 P. R. China China-Japan Union Hospital Jilin University Changchun 130033 P. R. China

The components of the essential oils obtained from different parts of Daacus carota ***. saliva Hoffm were analyzed. The percentages of the essential oils extracted are 0. 27% （ mL/100 g material） for the flowers, 0. 0/% for the stems and leaves and 0. 01% for the roots. Fifty-four, Sixty-six and Thirty-three compounds were,respectively, separated and identified from the flowers, the stems and leaves and the roots, among which unsaturated alkene compounds are thirty-nine, thirty-nine and twenty-one, respectively, accounting in turn for up to 90. 21%, 90. 49% and 72. 65% of the total essential oils. Because alkene compounds have double bonds that are easily oxidized, it can he inferred that the components of the essential oils in the different parts of Daucus carota L. var. saliva Hoffm should show an activity of the antioformation of free radicals to some extent.

关键词： Daucus carota L. var. sativa Hoffm Essential oil. GC/MS

Synergistic Effects of Activin A and Fibroblast Growth Factor 2 in the Modulation of Insulin Expression

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Chemical Research in Chinese Universities 2006年第2期22卷 229-232页

作者： BAO Yong-li JIANG Hong-yu Ji Shou-xian WU Yin meng xiang-ying LI Yu-xin Institute of Genetics and Cytology Northeast Normal University Changchun 130024 P. R. China The First Affiliated Hospital Jilin University Changchun 130021 P. R. China The Second Affiliated Hospital Jilin University Changchun 130041 P. R. China

Diabetes is the most prevalent and serious metabolic disease, and the number of diabetic patients worldwide is increasing. The reduction of insulin biosynthes is in pancreatic E-cells is closely associated with the onset and progression of diabetes, therefore, it is important to search for ways to induce insulin-producing cells in non-E-cells. In the present study, it has been reported that activin A and a basic fibroblast growth factor 2 （ FGF2）, can synergistically increase the insulin mRNA level, in both mouse El4 striatal primary cell cultures and the hippocampal neuronal cell line HT22. Activin A and FGF2 can jointly stimulate the nuclear translocation of Smad3 and specifically activate ERK1/2. It is interesting to note that a specific inhibitor for MEK, U0126, can efficiently block the induction of an insulin promoter activity by activin A and FGF2. This indicates that activin A collaborates with FGF2, giving a signal to induce the insulin gene through selective activation of the ERK-type MAP kinase and Smad3 in mouse striatal and HT22 cells. These data suggest that activin A may act in concert with FGF2 for the development of insulin -positive neurons

关键词： Activin A Fibroblast growth factor 2 (FGF2) Insulin

Analysis of Essential Oils from Flower-buds, Leaves and Stems of Filipendula palmata(Pall.) Maxim.

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Chemical Research in Chinese Universities 2005年第6期21卷 658-662页

作者： WU Yin meng xiang-ying LIU Xiao-hua BAO Yong-li WANG Shu-ping LI Yu-xin Institute of Genetics and Cytology Northeast Normal University Changchun 130024 P. R. China

The essential oils of the flower-buds, leaves and stems of Filipendula palmata（ Pall. ） Maxim. were analyzed by GC and GC-MS. Thirty-three, forty-seven and forty-seven compounds in the flower-buds, leaves and stems were identified, respectively. Methyl salicylate exists in a great amount which is up to 70. 10% in the flower-buds. Its amount is also high in other two parts. The data obtained show that it may be one of the main natural mosquito-expelling and pain-alleviating components in the three parts. The flower-buds are the main active part with the mosquito-expelling function.

关键词： Filipendula palmata( Pall. ) Maxim. Essential oil GC-MS

Isolation and Characterization of Proteins Interacting with Activin Type Ⅱ Receptors

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Chemical Research in Chinese Universities 2006年第2期22卷 217-220页

作者： LIU Biao BAO Yong-li WEI Zhuang WU Yin meng xiang-ying LI Yu-xin YIN Wei-tian Department of Hand Surgery China-japan Union Hospital Jilin University Changchun 130031 P. R. China Institute of Genetics and Cytology Northeast Normal University Changchun 130024 P. R. China

Regulation of the number of aetivin receptors that are present in the cell membrane plays a key role in the modulation of cellular responses to activin. In order to find the regulators, a novel protein ARIPzip, interacting with activin type II receptors, was searched and identified by using yeast two-hybrid screening. ARIPzip is a splicing variant of ARIP2. This has been discussed previously. ARIPzip can specifically interact with ActR Ⅱ A, and is widely distributed in mouse tissues. Overexpression of ARIPzip can cause the activin-induced transcriptional activities to increase in a dose-dependent manner while the overexpression of ARIV2 can decrease these activities. These data suggest that the C-terminal rezions of ARIP2 and ARIPzip are involved in the regulation of activin signaling.

关键词： Activin Activin receptor Ⅱ A( ActR Ⅱ A) Activin receptor interaction protein(ARIP)

Preparation of Polyclonal Antibodies Against Testis-specific Protease 50 and Characterization of Antibody Specificity

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Chemical Research in Chinese Universities 2006年第3期22卷 308-311页

作者： ZHANG Jing YANG Ming XU Ze-Li BAO Yong-li WU Yin WANG Yue-zeng meng xiang-ying JU Xing-da GU Yang LI Yu-xin Research Center of Agriculture and Medicine Gene Engineering of Ministry of Education Institute of Genetics and Cytology Northeast Normal University Changchun 130024 P. R. China China-Japan Union Hospital Jilin University Changchun 130031 P. R. China

Testis-specific protease 50 （TSP50） is a testis-specific oncogene, which is abnormally activated in most tested patients with breast cancer. This property makes it an attractive molecular marker and a promising target for the diagnosis and therapy of breast cancer. In order to obtain the protective and specific polyclonal antibodies for further research, TSPS0 cDNA was amplified by RT-PCR from normal human testicular tissue, and inserted into eukaryotic expression vector PeDNA3.1. Rabbit anti-TSPS0 polyclonal antibodies were prepared by means of intramuscular injection of peDNA3.1-TSPS0 into the rabbits. Titem of the anti-sera were measured by ELISA and Western blotting with the E. coli cell lysate containing the induced GST-TSPS0 fusion protein as an antigen. In addition, we examined the expression of TSPS0 in both breast cancer cell line MCF-7 and breast cancer tissue by immunofluorescent and immunohistochemistry analysis.

关键词： Breast cancer ELISA Polyclonal antibody TSPSO

Treatment of microalgae in ballast water using hydroxyl radical based on the D-2 ballast water discharge standard

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Treatment of microalgae in ballast water using hydroxyl radi...

Treatment of 10t/h Ballast Water in Oceanic Ships Using hydroxyl radical（·OH） Based on Strong Electric-Field Discharge

中国物理学会第十八届全国静电学术会议

作者： meng xiang-ying CHEN Cao BAI Min-dong SUN Jian meng Fan-peng Institute of Marine Engineering Department of Physics Dalian Maritime University College of Environment and Ecology Xiamen University

In this paper, the effect of hydroxyl radical（·OH） on microalgae in ballast water was experimentally *** the method of strong ionization discharge of dielectric barrier, large numbers of ·OH were produced to be dissolved into ballast water to kill the *** the concentration of total residual antioxidant（TRO） was about 1.9 mg/L, the maximum concentration of killed algae was about 12 000 cell/m L. The microalgae did not reactivate when detected at 48 hours and 120 hours, respectively. The contents of chlorophyl-a, chlorophyl-b and chlorophyl-c were decreased by 50%–80%, which is primary reason leading the death of microalgae. The water quality was back to normal level after 120 hours which was no secondary pollution to aquatic organisms and *** results indicated that the D-2 ballast water discharge standard of IMO was satisfied and ·OH is an ideal green medicamen for killing microalgae in the course of conveying ship’s ballast water.

关键词： hydroxyl radical(·OH) strong ionization discharge microalgae chlorophyll water quality

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Treatment of 10t/h Ballast Water in Oceanic Ships Using hydr...