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Germline promoter hypermethylation of tumor suppressor genes in gastric cancer

World Journal of Gastroenterology 2012年第1期18卷 70-78页

作者： Pu-Yuan Wu Zheng Zhang Jing-mei Wang Wen-Wen Guo Nong Xiao Qiong He Ya-Ping Wang yi-mei Fan Department of Medical Genetics Medical SchoolNanjing University Nanjing 210093 Jiangsu Province China Jiangsu Key Laboratory of Molecular MedicineNanjing 210093 Jiangsu Province China Department of Pathology Drum Tower Hos-pital Affiliated to Medical School Nanjing University Nanjing210093 Jiangsu Province China Departmem of Pathology Lujiang Hos-pital Lujiang Chaohu 231500 Anhui Province China

AIM: To explore germline hypermethylation of the tumor suppressor genes MLH1, CDH1 and P16INK4a in suspected cases of hereditary gastric cancer (GC). METHODS: A group of 140 Chinese GC patients in whom the primary cancer had developed before the age of 60 or who had a familial history of cancer were screened for germline hypermethylation of the MLH1, CDH1 and P16INK4a tumor suppressor genes. GenomicDNA was extracted from peripheral blood leukocytes and modified by sodium bisulfite. The treated DNA was then subjected to bisulfi te DNA sequencing for a specif ic region of the MLH1 promoter. The methylation status of CDH1 or P16INK4a was assayed using methylation-specif ic PCR. Clonal bisulf ite allelic sequencing in positive samples was performed to obtain a comprehensive analysis of the CpG island methylation status of these promoter regions. RESULTS: Methylation of the MLH1 gene promoter was detected in the peripheral blood DNA of only 1/140 (0.7%) of the GC patient group. However, this methylation pattern was mosaic rather than the allelic pattern which has previously been reported for MLH1 in hereditary non-polyposis colorectal cancer (HNPCC) patients. We found that 10% of the MLH1 alleles in the peripheral blood DNA of this patient were methylated, consistent with 20% of cells having one methylated allele. No germline promoter methylation of the CDH1 or P16INK4a genes was detected. CONCLUSION: Mosaic germline epimutation of the MLH1 gene is present in suspected hereditary GC patients in China but at a very low level. Germline epimutation of the CDH1 or P16INK4a gene is not a frequent event.

关键词： Gastric cancer Germline promoter methylation MLH1 CDH1 P16INK4a

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A new triterpenoid saponin from the leaves and stems of Panax quinquefolium L.

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Chinese Chemical Letters 2009年第10期20卷 1207-1210页

作者： Guo Yu Li yi mei Zeng He Meng Xian Li Jin Hui Wang School of Pharmacy Shihezi University Shihezi 832002 China School of Traditional Chinese Materia Medica. Shenyang Pharmaceutical University Shenyang 110016 China

One new triterpcnoid saponin, quinquenoside L17 （1）, was isolated from the leaves and stems of Panax quinquefolium L., and its structure was elucidated as 20-O-[（β-D-xylopyranosyl-（1-6）-O-β-D-glucopyranosy）]-6-O-β-D-glucopyranosy1-dammar-24-ene- 3,6,12,20-tetraol, by the combination analysis of one-dimensional NMR and two-dimensional NMR, mass spectrometry, CD spectrum and chemical evidences.

关键词： Panax quinquefolium L. Quinquenoside L17 Triterpenoid saponin

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Formation and Control of Sharp {100}<021> Texture in Electrical Steel

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Journal of Iron and Steel Research International 2006年第2期13卷 54-58页

作者： WANG Jun-an ZHOU Bang-xin YAO mei-yi LI Qiang CHEN Wen-jue Institute of Materials Shanghai UniversityShanghai 200072China

The formation and control of sharp{100}〈021〉texture in electrical steels were experimentally investigated and the main influencing factors were *** steels of a base composition of Fe-3.2%Si with a certain amount of carbon and manganese were employed to obtain{100}〈021〉texture through one stage cold rolling and *** results indicated that by careful control of the amount of alloying elements and the annealing temperature,a relatively sharp{100}〈021〉texture in fairly thick sheets is *** primary recrystallization,a weak component of{100}〈021〉and strong component of(447)〈18114〉texture,which constitute∑9 coincidence site lattice boundaries with a common〈011〉axis for 39°difference,are *** special grain boundary promotes the growth of{100}〈021〉grains in addition to the lower surface energy of(100)grains during secondary recrystallization.

关键词： {100}〈021〉texture electrical steel recrystallization grain growth

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Surface characteristics of regenerative Fe-KOH/LSB catalysts for low-temperature catalytic hydrolysis of carbon disulfide and research of the surface regeneration mechanism

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Frontiers of Materials Science 2018年第4期12卷 426-437页

作者： Chi WANG Kai LI Xin SUN yi mei Xin SONG Ping NING Lina SUN Faculty of Chemical Engineering Kunming University of Science and TechnologyKunming 650500China Faculty of Environmental Science and Engineering Kunming University of Science and TechnologyKunming 650500China

In the present study, a series of regeneration conditions and the regeneration mechanism of modified lake sediment biochar (Fe-KOH/LSB) catalysts for low-temperature catalytic hydrolysis of carbon disulfide (CS2) were investigated. The results showed that Rm-WNA method had the best regeneration effect. Under optimal regeneration conditions, the sulfur capacity (13.86 mg[S]/g[catalyst]) of regenerated Fe- KOH/LSB was close to that of fresh Fe-KOH/LSB (14.88 mg[S]/g[catalyst]). The water washing process could wash away a small number of sulfates and a large number of alkaline groups. TG-DTA and DRTFIR results indicated that the nitrogen sweeping process could decompose Fe2(SO4)3 into Fe2O3, which partially recovered the catalytic and the adsorptive abilities. CO2-TPD results indicated that the alkali steeping process offer -OH groups, further improving the catalytic and the adsorptive abilities. After 3 times-regeneration, the sulfur capacity of Fe-KOH/LSB reached 13.31 mg[S]/g[catalyst], indicating that the Rm-WNA method had good stability for the recovery of the catalytic activity. BET, XPS and XRD results revealed that the decrease of the sulfur capacity for regeneration was attributed to the decrease of the adsorptive abilities of C and SiO2.

关键词： regeneration condition modified lake sediment regeneration mechanism biochar catalytic hydrolysis carbon disulfide

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Pure transvaginal natural orifice transluminal endoscopic surgery right hemicolectomy for colon cancer:A case report

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World Journal of Clinical Cases 2021年第7期9卷 1714-1719页

作者： Zi-Jia Song yi-Qing Shi yi-mei Jiang Kun Liu You Li Chang-Gang Wang Ren Zhao Department of General Surgery Ruijin HospitalShanghai Jiaotong University School of MedicineShanghai 201801China

BACKGROUND Pure natural orifice transluminal endoscopic surgery(NOTES)for colorectal cancer is a complex procedure and rarely used in clinical practice because of the ethical concerns and technical challenges,including loss of triangulation,in-line orientation,and instrument ***(v)NOTES,however,can overcome these technical *** report a case of pure vNOTES right hemicolectomy for colon cancer,attached with surgical *** SUMMARY A 65-year-old woman with a 2-year history of intermittent diarrhea was diagnosed with ascending colon adenocarcinoma by colonoscopy and *** vNOTES right hemicolectomy was performed with complete mesocolic excision by well-experienced *** operative time was 200 min and the estimated blood loss was 30 *** intraoperative or postoperative complications occurred within 30 d after the *** visual analog scale pain score on postoperative day 1 was 1 and dropped to 0 on postoperative days 2 and *** patient was discharged at postoperative day *** pathologic specimen had sufficient clear resection margins and 14 negative harvested lymph *** vNOTES right hemicolectomy,performed by well-experienced surgeons,overcomes the technical challenges of pure NOTES and may be feasible for colon cancer.

关键词： Natural orifice transluminal endoscopic surgery Colon cancer Right hemicolectomy Complete mesocolic excision Surgical video Case report

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Effects of nursing care in fast-track surgery on postoperative pain, psychological state, and patient satisfaction with nursing for glioma

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World Journal of Clinical Cases 2021年第20期9卷 5435-5441页

作者： Yan-Hong Deng yi-mei Yang Jian Ruan Lin Mu Shi-Qiang Wang Department of Neurosurgery Chongqing University Cancer HospitalChongqing 400030China Sterile Supply Center Chongqing University Cancer HospitalChongqing 400030China Department of Anesthesiology Chongqing University Cancer HospitalChongqing 400030China

BACKGROUND The brain is the most complex organ in the human *** for a glioma always involves a multi-disciplinary *** care in fast-track surgery or enhanced recovery after surgery is such kind of work implemented by an interdisciplinary team to provide services to patients to improve their *** To explore the effects of nursing care in fast-track surgery on postoperative pain,psychological state,and patient satisfaction with nursing for *** From June 2018 to June 2020,138 patients who underwent operation for glioma at Cancer Hospital Affiliated to Chongqing University were *** were categorized into groups according to different nursing care that they *** them,69 patients receiving nursing care in fast-track surgery were included in an experimental group,and 69 patients receiving conventional postoperative nursing were included in a control *** analogue scale was used to evaluate postoperative pain in the two groups immediately after the operation and at 3 d after the ***-rating anxiety scale(SAS)and self-rating depression scale(SDS)were used to evaluate the psychological status of patients immediately after operation and on the 3rd postoperative day.A self-made satisfaction scale for patient satisfaction with nursing was used to evaluate and compare patient satisfaction with nursing between the two *** Time to excretion,time to out-of-bed activities,and length of hospital stay were significantly shorter in the observation group than in the control group(P0.05).There was no significant difference in postoperative pain score between the two groups(P>0.05).The pain score was significantly lower in the observation group than in the control group at 3 d after the operation(P<0.05).There was no significant difference in postoperative SAS or SDS score between the

关键词： Nursing care in fast-track surgery Glioma Visual analogue scale Self-rating anxiety scale Self-rating depression scale

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Analysis of Simple Sequence Repeats in Genomes of Rhizobia

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Agricultural Sciences in China 2008年第10期7卷 1189-1195页

作者： GAO Ya-mei HAN yi-qiang TANG Hui SUN Dong-mei WANG Yan-jie WANG Wei-dong College of Life Science and Technology Heilongjiang August First Land Reclamation University Daqing 163319 P.R.China

Simple sequence repeats （SSRs） or microsatellites, as genetic markers, are ubiquitous in genomes of various organisms. The analysis of SSR in rhizobia genome provides useful information for a variety of applications in population genetics of rhizobia. We analyzed the occurrences, relative abundance, and relative density of SSRs, the most common in Bradyrhizobium japonicum, Mesorhizobium loti, and Sinorhizobium meliloti genomes se- quenced in the microorganisms tandem repeats database, and SSRs in the three species genomes were compared with each other. The result showed that there were 1 410, 859, and 638 SSRs in B. japonicum, M. loti, and S. meliloti genomes, respectively. In the genomes of B. japonicum, M. loti, and S. meliloti, tetranucleotide, pentanucleotide, and hexanucleotide repeats were more abundant and indicated higher mutation rates in these species. The least abundance was mononucleotide repeat. The SSRs type and distribution were similar among these species.

关键词： rhizobium genome simple sequence repeat

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Synthesis and Herbicidal Activity of Novel 5-Substituted Benzenesulfonylureas

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Chemical Research in Chinese Universities 2007年第6期23卷 674-678页

作者： WANG mei-yi MU Xiao-li GUO Wan-cheng LI Yong-hong LI Zheng-ming State Key Laboratory of Elemento-Organic Chemistry Research Institute of Elemento-Organic Chemistry Nankai University Tianfin 300071 P. R. China

Sulfonylurea herbicides have been widely used because of their low application rates, good crop sdeetivities and low mammalian toxicities. However, some sulfonylureas might persist unfavourably in the environment with residual problems. In order to look for ecologically safer and environmentally benign sulfonylureas, and on keeping the pyrimidine ring being monosubstitated, 15 novd C5-monosubstituted benzenesulfonylurea compounds were synthesized. The structures of all the compounds synthesized were confirmed by demental analysis and ^1H NMR. Preliminary herbicidal activities of these new sulfonylurea compounds were determined by ALS screening （ in vitro） and pot bioassay experiments（ in vivo）. The herbicidal results show that some novel sulfonylureas are comparable to commercial Foramsulfuron and Monosulfuron.

关键词： Sulfonylurea 5-Substituted phenyl ring Herbicidal activity

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Design of multiplex logic gates:combining regulation of DNA structure with logical calculation

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Science China Chemistry 2014年第3期57卷 453-458页

作者： TAO Jia ZHENG Jing LI JiShan ZHAO Peng LI JuanPing MA Cheng yi mei YANG RongHua State Key Laboratory of Chemo/Biosensing and Chemometrics College of Chemistry and Chemical Engineering Hunan University

In this paper,we proposed a facile and accurate way for controlling multiplex fluorescent logic gates through changing the exciting and the observing *** proof-of-principle,a Pb2+-specific DNAzyme probe and a thymine(T)-rich DNA probe were introduced to a double-stranded(ds-)*** addition style of the two ions served as the four inputs by changing the distance of the three fluorophores,6-carboxyfluorescein(FAM),ALEXA 532(ALEXA)and carboxytetramethylrhodamine(TAMRA),all of which were modified on the dsDNA *** with the previous methods,the present approach needed neither different inputs nor the change of sequence of the probe to achieve multiplex logic ***,the modularity of the strategy may allow it to be extended to other types of logic gates.

关键词： logic gates FRET DNA mercury ions(Ⅱ) lead ions

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Sources of varieties and quality of circular Fructus Ligustri Lucidi

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Chinese Journal of Natural Medicines 2016年第3期14卷 236-240页

作者： LI Yan-Peng WANG Si-Jia ZANG yi-mei HU Zhong-Sheng LIU Chun-Sheng College of Traditional Chinese Medicine Beijing University of Chinese MedicineBeijing 100102China Hu Qiao Pharmaceutical Co.Engineering Research Center Bozhou 236000China

This study aimed to trace sources and quantitatively analyze the specnuezhenide content of circular Fructus Ligustri Lucidi for clinical use. Different specifications of Fructus Ligustri Lucidi were identified using DNA barcoding technology and the specnuezhenide content was analyzed by High Performance Liquid Chromatography(HPLC). The ITS sequence of circular Fructus Ligustri Lucidi was identical to that of standard privet, which was determined through botanical identification. ITS sequence similarity between circular Fructus Ligustri Lucidi and Fructus Ligustri Lucidi which was registered in NCBI ranged from 99.5% to 100%. The sequences of circular and other Fructus Ligustri Lucidi were clustered in a Neighbor-Joining tree with bootstrap value of 95, and these sequences could be distinguished from adulterants. Conforming to pharmacopoeia standard, the average specnuezhenide content of circular Fructus Ligustri Lucidi was higher than that of chicken waist Fructus Ligustri Lucidi. Circular Fructus Ligustri Lucidi derived from Ligustrum lucidum Ait. and the specnuezhenide content was higher in circular Fructus Ligustri Lucidi than that in chicken waist Fructus Ligustri Lucidi.

关键词： Fructus Ligustri Lucidi Specifications DNA barcoding Specnuezhenide

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