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Effects of polar cortical cytoskeleton and unbalanced cortical surface tension on intercellular bridge thinning during cytokinesis

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Acta Mechanica Sinica 2011年第6期27卷 1081-1090页

作者： li Wang mei-wen An Xiao-Na li Fang Yang Yang liu Institute of Applied Mechanics and Biomedical Engineering Taiyuan University of Technology 030024 Taiyuan China

To probe the contributions of polar cortical cytoskeleton and the surface tension of daughter cells to intercellular bridge thinning dynamics during cytokinesis,we applied cytochalasin D（CD） or colchicine（COLC） in a highly localized manner to polar regions of dividing normal rat kidney（NRK） *** observed cellular morphological changes and analyzed the intercellular bridge thinning trajectories of dividing cells with different polar cortical *** blebbistatin（BS） application was used to obtain cells losing active contractile force *** results show that locally released CD or colchicine at the polar region caused inhibition of cytokinesis before *** treatment at phases after ingression allowed completion of cytokinesis but dramatically influenced the trajectories of intercellular bridge *** single polar cortical actin induced transformation of the intercellular bridge thinning process,and polar cortical tension controlled deformation time of intercellular *** study provides a feasible framework to induce and analyze the effects of local changes in mechanical properties of cellular components on single cellular cytokinesis.

关键词： Cytokinesis Normal rat kidney epithelial cells Intercellular bridge Polar cortical cytoskeleton - Cortical surface tension

Differential expression of Rab27A/B correlates with clinical outcome in hepatocellular carcinoma

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World Journal of Gastroenterology 2012年第15期18卷 1806-1813页

作者： Wei-Wei Dong Quan Mou Jian Chen Jian-Tao Cui wen-mei li wen-Hua Xiao Department of Oncology the First Affiliated Hospital the General Hospital of Chinese People＇s Liberation Army Beijing 100048 China Department of surgery 252 Hospital of Chinese People＇s Liberation Army Baoding 071000 Hebei Province China Laboratory of Molecular Oncology Peking University Cancer Hospital/Institute Beijing 100142 China

AIM:To investigate the association of Rab27A and Rab27B expression with clinicopathological characteristics and prognosis of hepatocellular carcinoma(HCC).METHODS:We used reverse transcription polymerase chain reaction(RT-PCR),real-time PCR,and Western blotting to detect Rab27A and Rab27B mRNA and protein expression in 5 human HCC lines and the immortalized hepatic HL-7702 cell *** further examined 148 primary HCC samples matched with adjacent normal tissue and 80 non-HCC specimens by immunohistochemistry to evaluate the correlation of Rab27A and Rab27B expression with clinicopathological features and ***:Our data showed that Rab27A and Rab27Bwere differentially expressed in cell lines and primary HCC ***27A mRNA and protein were detected in 67%(4/6)of human cell lines and 80%(4/5)of HCC cell lines,while Rab27B was found in 50%(3/6)of human lines and 40%(2/5)of HCC ***27A expression was higher in primary HCC(46.2%,66/143)than in matched adjacent tissue(24.3%,33/136,Plinicopathological characteristics of 148 HCC specimens revealed significant correlations between Rab27A and Rab27B expression and tumor tumor-node-metastasis(TNM)classification(P=0.046 and P=0.027,respectively),and between strong Rab27A expression and tumor differentiation grade(P=0.008).Survival analyses revealed that patients with Rab27A+or Rab27B+tumors had significantly reduced overall survival compared with that of patients with Rab27A-or Rab27B-tumors(P= 0.015 and P=0.005,respectively).Risk analyses revealed that Rab27B+and TNMⅢ-Ⅳwere independent poor prognosis factors associated with a 3.36-and 3.37fold higher relative risk of death,***:Rab27A and Rab27B expression were closely correlated with tumor progression and can be valuable prognostic indicators for HCC patients.

关键词： Rab27A Rab27B Hepatocellular carcinoma Immunohisto-chemistry Prognosis

A new diterpene from the stems of Trigonostemon heterophyllus

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Chinese Journal of Natural Medicines 2014年第4期12卷 297-299页

作者： li Yi-Xing ZUO wen-Jian mei wen-li CHEN Hui-Qin DAI Hao-Fu Key Laboratory of Biology and Genetic Resources of Tropical Crops Ministry of Agriculture Institute of Tropical Bioscience and Biotechnology Chinese Academy of Tropical Agricultural Sciences Haikou Experimental Station Chinese Academy of Tropical Agricultural Sciences

AIM: To investigate the chemical constituents in the stems of Trigonostemon heterophyllus. METHOD: The chemical constituents were isolated by column chromatography on silica gel, Rp-18, and Sephadex LH-20, and their structures were elucidated on the basis of spectroscopic analysis. RESULTS: Three compounds were isolated and identified as a new diterpene, trigonoheterene B(1), together with two known compounds, trigonostemone(2) and trigonochinene B(3). CONCLUSION: Compound 1 is new. Compounds 2 and 3 showed antibacterial activities.

关键词： Trigonostemon heterophyllus Trigonoheterene B Diterpene Antibacterial activities

Metabolites from the endophytic fungus HP-1 of Chinese eaglewood

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Chinese Journal of Natural Medicines 2014年第2期12卷 151-153页

作者： ZUO wen-Jian JIN Peng-Fei DONG wen-Hua DAI Hao-Fu mei wen-li Key Laboratory of Biology and Genetic Resources of Tropical Crops Ministry of Agriculture Institute of Tropical Bioscience and Biotechnology Chinese Academy of Tropical Agricultural Sciences

AIM: To study the chemical constituents from the fermentation of the endophytic fungus HP-1 of Chinese eaglewood. METHODS: The chemical constituents were isolated by column chromatography on silica gel and Sephadex LH-20, and their structures were elucidated on the basis of spectroscopic analysis. RESULTS: Four compounds were isolated and identified as 3α, 3β, 10β-trimethyl-decahydroazuleno[6, 7]furan-8, 9, 14-triol(1), 4-hydroxyphenylacetic acid(2), 4-hydroxyphenethyl alcohol(3), and 5-hydroxymethyl-2-furancarboxaldehyde(4). CONCLUSION: Compound 1 was a new compound. Compound 2 showed antibacterial activity against Staphylococcus aureus.

关键词： Chinese eaglewood Metabolites Endophytic fungus Antibacterial activity Chaetomium sp.

Antioxidant and Cytotoxic Phenolic Compounds of Areca Nut(Areca catechu)

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Chemical Research in Chinese Universities 2010年第1期26卷 161-164页

作者： ZHANG Xing WU Jiao HAN Zhuang mei wen-li DAI Hao-fu Key Laboratory of Tropical Crop Bioteehnology Ministry of Agriculture Institute of Tropical Bioseience and Bioteehnology Chinese Academy of Tropical Agricultural Sciences Haikou 5 71101 P. R. China

Areca catechu L.（Palmae）, commonly known as an important economical seed crop, is widely culti- vated in tropical and subtropical areas, including India, Southeast Asia, East Africa and New Guinea. Areca nut（frequently known as betel nut） is the ripe fruit of the tree A. catechu. Areca nut can be chewed and it is a common masticatory in tropical and subtropical countries. It was estimated in the early 1990s that 10% to 20% of the world＇s population chewed betel quid daily. Areca nut is commonly used in folklore medicine for treatment of various diseases such as dyspep sia, constipation, beriberi and oedema.

关键词： Areca catechu Phenylpropanoid DPPH Antioxidant activity Cytotoxic activity

The Uniqueness of Decomposition of Symplectic Ternary Algebras with Trivial Center

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Journal of Mathematical Research and Exposition 2010年第3期30卷 423-428页

作者： Xi mei BAI wen li liU College of Mathematics and Computer Hebei University Hebei 071002 P. R. China

In this paper, the authors define the center of a Symplectic ternary algebra, and investigate the relationship between the center of a Symplectic ternary algebra and that of the lie triple system associated with it. As an application of the relationship, the unique decomposition theorem for Symplectic ternary algebras with trivial center is obtained.

关键词： Symplectic ternary algebras lie triple system center U-endomorphism.

A New Phenylpropanoid Glycoside from Dragon＇s Blood of Dracaena cambodiana

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Chemical Research in Chinese Universities 2013年第3期29卷 483-486页

作者： SHEN Hai-yan WANG Hui ZUO wen-jian ZHAO You-xing LUO Ying mei wen-li DAI Hao-fu Key Laboratory of Biology and Genetic Resources of Tropical Crops Ministry of Agriculture Institute of Tropical Bioscience and Biotechnology Chinese Academy of Tropical Agricultural Sciences Haikou 571101 P R. China Hainan Key Laboratory for Research and Development of Natural Products from li Folk Medicine Haikou 571101 P. R. China Horticultural and Garden College Hainan University Haikou 570228 P. R. China

A new phenylpropanoid glycoside, named cambodianin F（1）, together with three known compounds, 1-O-β-D-glucopyranosyl-2-hydroxy-4-allylbenzene（ 2 ）, 1-O-（ 6-O-a-L-rhamnopyranosyl-β-D-glucopyranosyl）-2-hydroxy- 4-allylbenzene（3） and 1,2-di-O-β-D-glucopyranosyl-4-allylbenzene（4） was isolated from the dragon＇s blood of Dracaena cambodiana. The new compound was elucidated by HR-ESI-MS and spectroscopic techniques（UV, IR, 1D and 2D NMR）.

关键词： Dragon's blood Dracaena cambodiana Cambodianin F

Effect of Sc on Al_(3)Fe phase and mechanical properties of as-cast AA5052 aluminum alloy

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China Foundry 2024年第3期21卷 257-264页

作者： Yang li Qing Yu Feng-feng Chen Jia-wen He Hong-mei Yang Meng-nie li Faculty of Materials Science and Engineering Kunming University of Science and TechnologyKunming 650093China

The AA5052 aluminum alloy is widely used in automobile and aerospace manufacturing,and with the development of light-weight alloys,it is required that these materials exhibit better mechanical *** studies have demonstrated that the addition of Sc to aluminum alloys can improve both the microstructure and properties of the *** this study,the effect of Sc on the Fe-rich phase and properties of the AA5052 aluminum alloy was studied by adding 0%,0.05%,0.2%,and 0.3%*** results show that with the increase of Sc,the coarse needle-like Fe-rich phase gradually transforms into Chinese-script and then nearly spherical particles,reduce the size of Fe-rich phase,and refine the grain with increase of high angle grain boundaries(HAGBs).These microstructure changes enhance the strength of the AA5052 alloy through Sc *** ductility of the alloy is obviously improved because the addition of a lower amount of Sc changes the morphology of Fe-rich phase from needle-like into a Chinese-script,and it is subsequently reduced as a result of significant increase in HAGBs with increasing Sc content.

关键词： AA5052 aluminum alloy Al3Fe phase mechanical properties grain boundary

Molecular Cloning and mRNA Profile of Insulin-like Growth Factor Type 1 Receptor in Orange-spotted Grouper,Epinephelus coioides

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Acta Biochimica et Biophysica Sinica 2005年第5期37卷 327-334页

作者： Yue-mei KUANG wen-Sheng li Hao-Ran liN Institute of Aquatic Economic Animals and Guangdong Provincial Key Laboratory for Aquatic Economic Animals Zhongshan University, Guangzhou 510275, China Institute of Aquatic Economic Animals and Guangdong Provincial Key Laboratory for Aquatic Economic Animals Zhongshan UniversityGuangzhou 510275 China

The insulin-like growth factor type 1 receptor(IGF-IR)belongs to the tyrosine kinase(TK)receptor *** being mitogenic,IGF-IR plays a crucial role in cell survival,transformation andmaintenance of the malignant *** this study,we cloned the cDNA from the hypothalamus of theorange-spotted grouper(Epinephelus coioides)using reverse transcription PCR(RT-PCR)and the rapidamplification of cDNA ends(RACE)*** deduced amino acid sequence showed that the receptorcomprises 1413 amino acid *** contains cysteine-rich domains in its α-subunit,and a conservedtransmembrane domain and TK domains in its β-*** of the amino acid sequence withthose of other species showed that the grouper IGF-IR shares 90.2%,89.6%,71.9% and 72% similaritywith the IGF-IR of the Japanese flounder,turbot,zebrafish-a and zebrafish-b,*** comparedwith its mammalian homologue,grouper IGF-IR contains a large insertion at its *** has revealed that the grouper IGF-IR belongs to the b-type IGF-IRs and has a higher similarity withflounder and turbot IGF-IR,and a lower similarity(liver,heart and thymus *** expression of IGF-IR can be visualizedas a ubiquitous signal in unfertilized eggs,embryos and early development *** distribution pattern ofIGF-IR mRNA in grouper development suggests that IGF-IR plays an important role in the embryo andearly larval development stages.

关键词： orange-spotted grouper(Epinephelus coioides) insulin-like growth factor type 1 receptor cDNA cloning mRNA expression