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Clinical application of free/total PSA ratio in the diagnosis of prostate cancer in men over 50 years of age with total PSA levels of 2.0-25.0 ng ml^(−1) in Western China

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Asian Journal of Andrology 2022年第2期24卷 195-200页

作者： Xue-Dan Gao Qiang Miao Jun-Long Zhang Jian-Zhao Zhai Xue-mei gui Yi-Han Cai Qian Niu Bei Cai Department of Laboratory Medicine/Research Center of Clinical Laboratory Medicine West China Hospital of Sichuan UniversityChengdu 610041China Department of Laboratory Medicine Nan’an Chinese Medicine HospitalChongqing 400060China

The goal of this study was to investigate the clinical application of free/total prostate-specific antigen(F/T PSA)ratio,considering the new broad serum total PSA(T-PSA)“gray zone”of 2.0–25.0 ng ml^(−1)in differential diagnosis of prostate cancer(PCa)and benign prostate diseases(BPD)in men over 50 years in Western China.A total of 1655 patients were included,528 with PCa and 1127 with *** T-PSA,free PSA(F-PSA),and F/T PSA ratio were *** operating characteristic curves were used to assess the efficiency of PSA and F/T PSA *** were 47.4%of cancer patients with T-PSA of 2.0–25.0 ng ml^(−1).When T-PSA was 2.0–4.0 ng ml^(−1),4.0–10.0 ng ml^(−1),and 10.0–25.0 ng ml^(−1),the area under the curve(AUC)of F/T PSA ratio was 0.749,0.769,and 0.761,*** best AUC of F/T PSA ratio was 0.811 when T-PSA was 2.0–25.0 ng ml^(−1),with a specificity of 0.732,a sensitivity of 0.788,and an optimal cutoff value of 15.5%.The AUC of F/T PSA ratio in different age groups(50–59 years,60–69 years,70–79 years,and≥80 years)was 0.767,0.806,0.815,and 0.833,respectively,and the best sensitivity(0.857)and specificity(0.802)were observed in patients over 80 *** T-PSA trend was in accordance with the Gleason score,tumor node metastasis(TNM)stage,and American Joint Committee on Cancer prognosis ***,the F/T PSA ratio can facilitate the differential diagnosis of PCa and BPD in the broad T-PSA“gray zone”.Serum T-PSA can be a Gleason score and prognostic indicator.

关键词： free/total prostate-specific antigen ratio gray zone prostate-specific antigen prostate cancer total prostate-specific antigen

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Melittin induces human gastric cancer cell apoptosis via activation of mitochondrial pathway

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World Journal of Gastroenterology 2016年第11期22卷 3186-3195页

作者： gui-mei Kong Wen-Hua Tao Ya-Li Diao Peng-Hua Fang Ji-Jun Wang Ping Bo Feng Qian Department of Jiangsu Key Laboratory of Integrated Traditional Chinese and Western Medicine for Prevention and Treatment of Senile Diseases Jiangsu Coinnovation Center for Prevention and Control of Important Animal Infectious Diseases and ZoonosesMedical School of Yangzhou University Department of Gastroenterology 1st Hospital of Yangzhou

AIM: To investigate the apoptotic effects of melittin on SGC-7901 cells via activation of the mitochondrial signaling pathway in ***: SGC-7901 cells were stimulated by melittin, and its effect on proliferation and apoptosis of was investigated by methyl thiazolyl tetrazolium assay, morphologic structure with transmission electron microscopy, annexin-V/propidium iodide double-staining assay, measuring mitochondrial membrane potential(MMP) levels, and analyzing reactive oxygen species(ROS) concentrations were analyzed by flow cytometry. Cytochrome C(Cyt C), apoptosis-inducing factor(AIF), endonuclease G(Endo G), second mitochondria-derived activator of caspases(Smac)/direct IAP binding protein with low isoelectric point(Diablo), and FAS were analyzed by western blot. The expression of caspase-3 and caspase-8 was measured using activity assay ***: Melittin was incubated at 1.0, 2.0, 4.0, or 6.0 μg/m L for 1, 2, 4, 6, or 8 h and showed a timeand concentration-dependent inhibition of SGC-7901 cell growth. Melittin induced SGC-7901 cell apoptosis, which was confirmed by typical morphological changes. Treatment with 4 μg/m L melittin induced early apoptosis of SGC-7901 cells, and the early apoptosis rates were 39.97% ± 3.19%, 59.27% ± 3.94%, and 71.50% ± 2.87% vs 32.63% ± 2.75% for 1, 2, and 4 h vs 0 h(n = 3, P < 0.05); the ROS levels were 616.53% ± 79.78%, 974.81% ± 102.40%, and 1330.94% ± 93.09% vs 603.74% ± 71.99%(n = 3, P < 0.05); the MMP values were 2.07 ± 0.05, 1.78 ± 0.29, and 1.16 ± 0.25 vs 2.55 ± 0.42(n = 3, P < 0.05); caspase-3 activity was significantly higher compared to the control(5492.3 ± 321.1, 6562.0 ± 381.3, and 8695.7 ± 449.1 vs 2330.0 ± 121.9), but the caspase activity of the non-tumor cell line L-O2 was not different from that of the control. With the addition of the caspase-3 inhibitor(Ac-DEVD-CHO), caspase-3 activity was significantly decreased compared to the control group(1067.0 ± 132.5 U/g vs 8695.7 ± 449.1 U/g). The expression

关键词： Melittin Gastric cancer Mitochondrial Apoptosis Cytochrome C

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The surface properties of aluminated meso–macroporous silica and its catalytic performance as hydrodesulfurization catalyst support

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Petroleum Science 2017年第2期14卷 424-433页

作者： Zhi-Gang Wang Jia-Ning Pei Sheng-Li Chen Zheng Zhou gui-mei Yuan Zhi-Qing Wang Guo-Qiang Ren Hong-Jun Jiang State Key Laboratory of Heavy Oil Processing College of Chemical Engineering China University of Petroleum Beijing 102249 People's Republic of China Shanghai Petrochemical Company of Sinopec Jinshan Shanghai 200540 People's Republic of China

Aluminated mesoporous silica was prepared by multiple post-grafting of alumina onto uniform mesoporous SiO2 ,which was assembled from monodisperse SiO2 ***（HDS）catalyst was prepared by loading Ni and Mo active components onto the aluminated uniform mesoporous SiO2 ,and its HDS catalytic performance was evaluated using hydrodesulfurization of dibenzothiophene as the probe reaction at 300℃ and 6.0 MPa in a tubular *** samples were characterized by N2 physisorption,scanning electronic microscopy,Fourier transform infrared spectrum,X-ray diffraction（XRD）,temperature-programmed desorption of ammonia（NH3-TPD）,^27Al nuclear magnetic resonance（^27Al-NMR）and high-resolution transmission electron microscopy（HRTEM）.The results showed that the Si–OH group content of SiO2 was mainly dependent on the pretreatment conditions and had significant influence on the activity of the Ni Mo *** surface properties of the aluminated SiO2 varied with the Al2O3-grafting *** after four cycles of grafting,the aluminated SiO2 behaved like amorphous *** addition,plotting of activity of Ni Mo catalysts supported on aluminated meso–macroporous silica materials against the Al2O3-grafting cycle yields a volcano curve.

关键词： Aluminum grafting Hydrodesulfurization Surface properties Catalyst support SiO2

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Expression of Aminopeptidase N1(APN1),the Main Receptor Protein for Bacillus thuringiensis CrylA Toxin from Helicoverpa armigera Larval Midgut in Trichoplusia ni cells

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Agricultural Sciences in China 2008年第3期7卷 329-335页

作者： CHANG Hong-lei LIANG Ge-mei WANG gui-rong YU Hong-kun GUO Yu-yuan WU Kong-ming State Key Laboratory for Biology of Plant Disease and Insect Pests Institute of Plant Protection Chinese Academy of Agricultural Sciences Beijing 100094 P.R.China

The aim of this article is to successfully express the Bt （Bacillus thuringiensis） toxin receptor protein located on the internal membrane of larval midgut of cotton bollworm （Helicoverpa armigera Hübner） within eukaryotic expression system, which is one of the key links for clarifying the relationship between receptor and Bt resistance. The fragments of aminopeptidase N1 （APN1） gene without signal peptide in the susceptible and the resistant H. armigera were cloned separately using PCR method, and were separately cloned into pUC 19 vector. After sequencing the gene, the fragments encoding for APN1 without signal peptide were cloned into the Bac-to-Bac baculovirus expression system with transfer vector pFastBacHTB under the polyhedron gene promoter. The recombinant transposing plasmid pFastBacHTB/APN1 was screened and then transformed into Escherichia coli DH10Bac. It was cultured in LB medium, which contained Te, Kan, Ge, X-gal, and IPTG. The resulting recombinant bacmid was transfected into cells of the insect Trichoplusia ni and recombinant baculoviruse was obtained. The lysate of cells infected with recombinant baculoviruse was analyzed by SDS-PAGE and blot analysis. The results showed that the recombinant baculoviruse was fully capable of expressing APN1. The APN1 gene successfully expressed in T. ni cell established the base for continuing the research on its function and relationshio of resistance with Bt.

关键词： APN1 baculovirus expression vector Trichoplusia ni expression

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Comparison of effects of obesity and non-alcoholic fatty liver disease on incidence of type 2 diabetes mellitus

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World Journal of Gastroenterology 2015年第32期21卷 9607-9613页

作者： Wei-Dong Li Kun-Fa Fu gui-mei Li Yan-Shu Lian Ai-Min Ren Yun-Jue Chen Jin-Rong Xia Department of Gastroenterology Zhongda Hospital School of Medicine Southeast University Jiangsu Provincial Geriatric Hospital Department of Pathology 81~(st) Hospital of the People’s Liberation Army Department of Preventive Medicine Jiankang Vocational College Physical Examination Center Nanjing Branch Jiangsu Armed Police General Hospital Occupational Health Management Center Nanjing Hospital of Occupational Disease Control

AIM: To compare and analyze the effects of obesity and non-alcoholic fatty liver disease(NAFLD) on the incidence of type 2 diabetes mellitus(T2DM) in Chinese ***: In 2008, a population of 4847 subjects was randomly sampled from 17 medical units for enrollment in this cohort study. Baseline information was obtained via a questionnaire on general information, physical examination(height, weight, and blood pressure), laboratory tests(triglycerides, total cholesterol, fasting blood glucose, alanine aminotransferase(ALT), uric acid, and creatinine), B-mode ultrasound, and ECG screening. The incidence of T2 DM after four years of follow-up was calculated. Numeric variable data was tested for normality, with the data expressed as mean ± SD. Kaplan-meier analysis was performed to calculate the cumulative incidence. The Cox proportional hazards model was used to analyze the relative risk(RR) of different body mass index(BMI) levels and NAFLD on T2 DM, as well as analyzingthe RR adjusted for age, sex, blood pressure, lipids, transaminases, uric acid, and ***: A total of 4736(97.71%) subjects completed 4-year follow-up, with a median follow-up time of 3.85 years, totaling 17223 person-years. 380 subjects were diagnosed with T2 DM, with a cumulative incidence of 8.0%. The cumulative incidence of T2 DM in the NAFLD and control groups was 17.4% vs 4.1%(P < 0.001), respectively, while the incidence in overweight and obese subjects was 11.0% vs 15.8%(P < 0.001), respectively. The incidence of T2 DM increased with an increase in baseline BMI. Cox regression analysis showed that the risk of T2 DM in the NAFLD group(RR = 4.492, 95%CI: 3.640-5.542) after adjustment for age, sex, blood pressure, lipids, ALT, uric acid, and creatinine was 3.367(2.367-4.266), whi le t he value(RR, 95%CI) in overweight and obese subjects after adjustment for age, sex, BMI, blood pressure, lipids and other factors was 1.274(0.997-1.629) and 1.554(1.140-2.091), respectively. Strat

关键词： Non-alcoholic fatty liver disease Type 2 diabetes

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Mapping QTLs on BTA6 affecting milk production traits in a Chinese Holstein population

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Chinese Science Bulletin 2005年第16期50卷 1737-1742页

作者： CHEN Huiyong ZHANG Qin WANG Chunkao SHU Juan mei gui YIN Cengceng HU Fang XU Jingjing GONG Weijia LI Hejun QIU Xiaotian Key Laboratory of Animal Genetics and Breeding of the Ministry of Agriculture College of Animal Science and Technology China Agricultural University Beijing 100094. China College of Animal Science and Technology. China Agricultural University Beijing 100094 China

A Chinese Holstein population with daughter design was analyzed using 14 microsatellites covering a map distance of 55.7 cM on chromosome 6 to fine map QTL for five milk production traits. 26 paternal half-sib families with 2356 daughters were involved. Two different approaches, linear regression approach and variance component ap-proach, were employed, with a one-QTL model and two-QTL model fitted. With a one-QTL model, the linear regression approach revealed a QTL near BMS470 with effects on milk yield, fat yield, protein yield, and fat percentage, and another QTL near BMS2460 for protein percentage. The variance component approach confirmed the results of linear regres-sion approach for the three yield traits, with the exception that the QTL for fat yield was mapped to a different position near BMS1242. The 95% confidence intervals resulted from linear regression, obtained by bootstrapping, were generally large, ranging from 31 to 53 cM, whereas the variance com-ponent approach revealed very small confidence intervals, calculated by LOD drop-off method, for the three yield traits, only 4―5 cM. With a two-QTL model, both approaches pro-vided strong evidence for the existence of two QTLs for the three yield traits. Along with the QTLs identified in one-QTL model analyses, the linear regression approach revealed a second QTL near BP7 with effects on all the three yield traits, whereas the variance component approach located the sec-ond QTL near ILSS035, BMS470, and BP7 for the three traits, respectively.

关键词：中国荷兰乳牛乳制品变异结构食品安全

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基于自适应多尺度积阈值的钢轨裂纹红外图像增强

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铁道学报 2015年第2期37卷 58-63页

作者：顾桂梅黄涛兰州交通大学自动化与电气工程学院甘肃兰州730070

利用红外热波技术检测钢轨斜裂纹伤损所得的红外图像存在整体亮度偏暗、边缘对比度低和纹理细节模糊的缺点,对测量裂纹的扩展深度不利。对此,本文提出一种基于非子采样Contourlet变换NSCT(Nonsubsampled Contourlet Transform)的自适应... 详细信息

利用红外热波技术检测钢轨斜裂纹伤损所得的红外图像存在整体亮度偏暗、边缘对比度低和纹理细节模糊的缺点,对测量裂纹的扩展深度不利。对此,本文提出一种基于非子采样Contourlet变换NSCT(Nonsubsampled Contourlet Transform)的自适应多尺度积阈值AMPT(Adaptive Multiscale Products Thresholding)钢轨裂纹红外图像增强算法。对图像进行NSCT分解得到不同尺度不同方向上的子带系数,由子带系数自适应地确定阈值并调整增强函数,将阈值作用于每一子带的多尺度积,对阈值化操作后的子带系数进行增强处理和逆变换,实现图像增强。实验结果表明:该算法在抑制噪声和提高图像整体对比度的基础上,有效突出了目标图像的边缘,对裂纹内部微弱细节纹理的增强效果优于其他传统的红外图像增强方法,为进一步提取代表裂纹深度的关键像素提供了有力支撑。

关键词：裂纹红外图像自适应增强非子采样Contourlet变换多尺度积阈值

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Unexpected expression of heat-activated transient receptor potential(TRP)channels in winter torpid bats and cold-activated TRP channels in summer active bats

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Zoological Research 2022年第1期43卷 52-63页

作者： Yang-Yang Li Qing-Yun Lv Guan-Tao Zheng Di Liu Ji Ma gui-mei He Li-Biao Zhang Shan Zheng Hai-Peng Li Yi-Hsuan Pan Key Laboratory of Brain Functional Genomics of Shanghai and Ministry of Education School of Life SciencesEast China Normal UniversityShanghai 200062China School of Life Science Department of Biomedical ScienceEast China Normal UniversityShanghai 200062China Institute of Eco-Chongming School of Life SciencesEast China Normal UniversityShanghai 200062China Guangdong Key Laboratory of Animal Conservation and Resource Utilization Guangdong Public Laboratory of Wild Animal Conservation and UtilizationInstitute of ZoologyGuangdong Academy of SciencesGuangzhouGuangdong 510260China CAS Key Laboratory of Computational Biology Shanghai Institute of Nutrition and HealthChinese Academy of SciencesShanghai 200031China

The ability to sense temperature changes is crucial for mammalian *** thermal sensing is primarily carried out by thermosensitive transient receptor potential channels(Thermo-TRPs).Some mammals hibernate to survive cold winter conditions,during which time their body temperature fluctuates ***,the underlying mechanisms by which these mammals regulate thermal responses remain *** quantitative real-time polymerase chain reaction(qRT-PCR)and the Western blotting,we found that Myotis ricketti bats had high levels of heat-activated TRPs(e.g.,TRPV1 and TRPV4)during torpor in winter and cold-activated TRPs(e.g.,TRPM8 and TRPC5)during active states in *** also found that laboratory mice had high mRNA levels of cold-activated TRPs(e.g.,Trpm8 and Trpc5)under relatively hot conditions(i.e.,40°C).These data suggest that small mammals up-regulate the expression of cold-activated TRPs even under warm or hot *** site analysis showed that some homeobox(HOX)transcription factors(TFs)regulate the expression of hot-and cold-activated TRP genes and that some TFs of the Pit-Oct-Unc(POU)family regulate warm-sensitive and cold-activated TRP *** dual-luciferase reporter assay results demonstrated that TFs HOXA9,POU3F1,and POU5F1 regulate TRPC5 expression,suggesting that Thermo-TRP genes are regulated by multiple TFs of the HOX and POU families at different *** study provides insights into the adaptive mechanisms underlying thermal sensing used by bats to survive hibernation.

关键词： Hibernation Bats Brain Thermo-TRPs

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Microadenocarcinoma in the head of the pancreas

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Chinese Medical Journal 2007年第20期120卷 1853-1854页

作者： LANG Zhi-qiang QU gui-mei YAO Wei-dong JIANG Lei Department of Pathology Yantai Yuhuangding Hospital Medical College of Qingdao University Yantai 264000 China

Microadenocarcinoma （MA） is a rare variant of ductal carcinoma of the pancreas. Although it is generally accepted that the MA is a pattern of owth, some researchers regard it as a distinctive entity. Here we report ... 详细信息

关键词： microadenocarcinoma pancreas

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Microcalorimetry studies of the antimicrobial actions of Aconitum alkaloids

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2015年第8期16卷 690-695页

作者： Yan-bin SHI Lian LIU Wei SHAO Ting WEI gui-mei LIN School of Mechanical and Automotive Engineering Qilu University of Technology Beijing Health Vocational College School of Pharmaceutical Science Shandong University

The metabolic activity of organisms can be measured by recording the heat output using microcalorimetry. In this paper, the total alkaloids in the traditional Chinese medicine Radix Aconiti Lateralis were extracted and applied to Eschenchia coil and Staphylococcus aureus. The effect of alkaloids on bacteda growth was studied by microcalorimetry. The power-time curves were plotted with a thermal activity monitor （TAM） air isothermal microcalorimeter and pa- rameters such as growth rate constant （p）, peak-time （Trn）, inhibitory ratio （I）, and enhancement ratio （E） were cal- culated. The relationships between the concentration of Aconitum alkaloids and p of E. coil or S. aureus were discussed. The results showed that Aconitum alkaloids had little effect on E. coil and had a potentially inhibitory effect on the growth of S. aureus.

关键词： Aconitum alkaloids Escherichia coil Staphylococcus aureus Microcalorimetry

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