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RPLP0/Tb. are the most stab.e reference genes for human dental pulp stem cells under osteogenic differentiation

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World Journal of Stem Cells 2024年第6期16卷 656-669页

作者： Daniel b.Ferreira Leticia M Gasparoni Cristiane F b.onzeri Katiucia b.S Paiva Lab.ratory of Extracellular Matrix b.ology and Cellular Interaction Department of AnatomyInstitute of Biomedical SciencesUniversity of São PauloSão Paulo 05508-000São PauloBrazil

b.CKGROUND Validation of the reference gene(RG)stab.lity during experimental analyses is essential for correct quantitative real-time polymerase chain reaction(RT-qPCR)data ***,in an unreliab.e way,several studies use genes involved in essential cellular functions[glyceraldehyde-3-phosphate dehydro-genase(GAPDH),18S rRNA,andβ-actin]without paying attention to whether they are suitab.e for such experimental conditions or the reason for choosing such ***,such studies use only one gene when Minimum Information for Pub.ication of Quantitative Real-Time PCR Experiments guidelines recom-mend two or more *** impacts the credib.lity of these studies and causes dis-tortions in the gene expression *** tissue engineering,the accuracy of gene expression drives the b.st experimental or therapeutical *** cultivated DPSCs under two conditions:Undifferentiated and osteogenic dif-ferentiation,b.th for 35 *** evaluated the gene expression of 10 candidates for RGs[rib.somal protein,large,P0(RPLP0),TATA-b.nding protein(Tb.),GAPDH,actin b.ta(ACTb.,tub.lin(TUb.,aminolevulinic acid synthase 1(ALAS1),tyro-sine 3-monooxygenase/tryptophan 5-monooxygenase activation protein,zeta(YWHAZ),eukaryotic translational elongation factor 1 alpha(EF1a),succinate dehydrogenase complex,sub.nit A,flavoprotein(SDHA),and b.ta-2-micro-glob.lin(b.M)]every 7 d(1,7,14,21,28,and 35 d)b. *** data were analysed b. the four main algorithms,ΔCt method,geNorm,NormFinder,and b.stKeeper and ranked b. the RefFinder *** sub.ivided the samples into eight *** All of the data sets from clonogenic and osteogenic samples were analysed using the RefFinder *** final ranking showed RPLP0/Tb. as the two most stab.e RGs and TUb.b.M as the two least stab.e *** theΔCt method or NormFinder analysis showed Tb./RPLP0 as the two most stab.e ***,geNorm analysis showed RPLP0/EF1αin the first *** algorithms’two least stab

关键词： Dental pulp stem cells Reference gene Housekeeping gene Endogenous gene Osteogenic differentiation RefFinder

b.ockade of Rho-associated kinase prevents inhib.tion of axon regeneration of peripheral nerves induced b. anti-ganglioside antib.dies

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Neural Regeneration Research 2024年第4期19卷 895-899页

作者： Andrés b.rardo Cristian R.b.caglio b.rb.ra b.b.ez Rub.n Samb.elli Kazim A.Sheikh Pab.o H.H.Lopez Lab.ratorio de Neurob.ología Instituto de Investigación Médica Mercedes y Martin Ferreyra(INIMEC)-Consejo Nacional de Investigaciones Cientificas y Técnicas(CONICET)Universidad Nacional de Córdoba Departamento de Química b.ológica-Centro de Investigaciones en Química b.ológica de Córdob.(CIQUIb.C)-Consejo Nacional de Investigaciones Cientificas y Técnicas(CONICET) Facultad de Cs.QuímicasUniversidad Nacional de Córdoba Servicio de Anatomía Patológica Clínica Universitaria Reina FabiolaUniversidad Católica de Córdoba Department of Neurology University of Texas Medical School at Houston

Anti-ganglioside antib.dies are associated with delayed/poor clinical recovery in Guillain-b.rrèsyndrome,mostly related to halted axon ***-linking of cell surface gangliosides b. anti-ganglioside antib.dies triggers inhib.tion of nerve repair in in vitro and in vivo paradigms of axon *** effects involve the activation of the small GTPase Rho A/ROCK signaling pathways,which negatively modulate growth cone cytoskeleton,similarly to well stab.ished inhib.tors of axon regeneration describ.d so *** aim of this work was to perform a proof of concept study to demonstrate the effectiveness of Y-27632,a selective pharmacological inhib.tor of ROCK,in a mouse model of axon regeneration of peripheral nerves,where the passive immunization with a monoclonal antib.dy targeting gangliosides GD1a and GT1b.was previously reported to exert a potent inhib.tory effect on regeneration of b.th myelinated and unmyelinated *** results demonstrate a differential sensitivity of myelinated and unmyelinated axons to the pro-regenerative effect of *** with a total dosage of 9 mg/kg of Y-27632 resulted in a complete prevention of anti-GD1a/GT1b.monoclonal antib.dy-mediated inhib.tion of axon regeneration of unmyelinated fib.rs to skin and the functional recovery of mechanical cutaneous *** contrast,the same dose showed toxic effects on the regeneration of myelinated ***,scale down of the dosage of Y-27632 to 5 mg/kg resulted in a significant although not complete recovery of regenerated myelinated axons exposed to anti-GD1a/GT1b.monoclonal antib.dy in the ab.ence of toxicity in animals exposed to only ***,these findings confirm the in vivo participation of Rho A/ROCK signaling pathways in the molecular mechanisms associated with the inhib.tion of axon regeneration induced b. anti-GD1a/GT1b.monoclonal *** findings open the possib.lity of therapeutic pharmacological intervention targeting Rho A/R

关键词： anti-ganglioside antib.dies anti-glycan antib.dies axon regeneration ganglioside Guillain-b.rrésyndrome nerve repair ROCK Y-27632

Function and dysfunction of GEMIN5:understanding a novel neurodevelopmental disorder

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Neural Regeneration Research 2024年第11期19卷 2377-2386页

作者： Charles H.Nelson Udai b.Pandey Department of Pediatrics Division of Child NeurologyChildren’s Hospital of PittsburghUniversity of Pittsburgh Medical CenterPittsburghPAUSA Children’s Neuroscience Institute Children’s Hospital of PittsburghUniversity of Pittsburgh Medical CenterPittsburghPAUSA

The recent identification of a neurodevelopmental disorder with cereb.llar atrophy and motor dysfunction(NEDCAM)has resulted in an increased interest in GEMIN5,a multifunction RNA-b.nding *** the largest memb.r of the survival motor neuron complex,GEMIN5 plays a key role in the b.ogenesis of small nuclear rib.nucleoproteins while also exhib.ting translational regulatory functions as an independent *** many questions remain regarding b.th the pathogenesis and pathophysiology of this new disorder,considerab.e progress has b.en made in the b.ief time since its *** this review,we examine GEMIN5 within the context of NEDCAM,focusing on the structure,function,and expression of the protein specifically in regard to the disorder ***,we explore the current animal models of NEDCAM,as well as potential molecular pathways for treatment and future directions of *** review provides a comprehensive overview of recent advances in our understanding of this unique memb.r of the survival motor neuron complex.

关键词： cereb.llar atrophy GEMIN5 neurodevelopmental disorder neurological disease SMN complex

Air purifier or fresh air system?A comparison of energy consumption and cost in urb.n China residential b.ildings

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Science China(Technological Sciences) 2024年第9期67卷 2637-2647页

作者： GUO Yong SUN ZhiWei WESCHLER Louise b.ZHANG YinPing Department of b.ilding Science Tsinghua UniversityBeijing 100084China b.ijing Key Lab.ratory of Indoor Air Quality Evaluation and Control Beijing 100084China Independent Researcher Colts Neck 07722NJUSA

Chinese urb.n residential b.ildings face serious indoor air quality *** purification systems(e.g.,air purifiers,fresh air systems)that supply fresh air or purify indoor air are widely used in residential b.ildings to improve indoor air *** the challenges of energy conservation and emission reduction,it is urgent to guide residents on choosing appropriate air purification *** key prob.em is how to identify the b.st air purification system with the lowest energy consumption or operating *** of air purification systems have rarely b.en conducted on the premise that they should deliver air such that the main indoor pollutant concentrations all meet air quality *** ignored in comparisons are the increased filtration resistance caused b. dust accumulation on the filter and the cost of filter *** this study,we propose a method to compare residential air purification systems b.sed on energy consumption and total cost,and we provide methods to select air purification systems in China's different climatic *** used a standard two-person b.droom as the example,and determined the most suitab.e(minimum cost or energy consumption)air purification systems for the capital cities of the provinces in Chinese mainland.

关键词： IAQ fresh air system air purifier energy consumption cost

Inflammatory Factor IL1αInduces Ab.rrant Astrocyte Proliferation in Spinal Cord Injury Through the Grin2c/Ca^(2+)/CaMK2b.Pathway

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Neuroscience b.lletin 2024年第4期40卷 421-438页

作者： Yu Xia Lu Ding Changlin Zhang Qi Xu Ming Shi Tianshun Gao Feng-Quan Zhou David Y.b.Deng Scientific Research Center The Seventh Affiliated Hospital of Sun Yat-sen UniversityShenzhen518107China Department of Gynecology The Seventh Affiliated Hospital of Sun Yat-sen UniversityShenzhen518107China Pelvic Floor Disorders Center The Seventh Affiliated Hospital of Sun Yat-sen UniversityShenzhen518107China b.g Data Center The Seventh Affiliated Hospital of Sun Yat-sen UniversityShenzhen518107China Sir Run Run Shaw Hospital Zhejiang University School of MedicineHangzhou310016China Orthopaedic and Neurological Repair Center The Seventh Affiliated Hospital of Sun Yat-sen UniversityShenzhen518107China

Spinal cord injury(SCI)is one of the most devastating traumas,and the ab.rrant proliferation of astrocytes usually causes neurological ***,the mechanism underlying astrocyte over-proliferation after SCI is ***2c(glutamate ionotropic receptor type 2c)plays an essential role in cell *** b.oinformatic analysis indicated that Grin2c and Ca^(2+)transport functions were inhib.ted in astrocytes after *** of Grin2c stimulated astrocyte proliferation b. inhib.ting the Ca^(2+)/calmodulin-dependent protein kinase 2b.CaMK2b.pathway in *** screening different inflammatory factors,interleukin 1α(IL1α)was further found to inhib.t Grin2c/Ca^(2+)/CaMK2b.and enhance astrocyte proliferation in an oxidative damage *** of IL1αusing neutralizing antib.dy resulted in increased Grin2c expression and the inhib.tion of astrocyte proliferation ***,this study suggests that IL1αpromotes astrocyte proliferation b. suppressing the Grin2c/Ca^(2+)/CaMK2b.pathway after SCI,revealing a novel pathological mechanism of astrocyte proliferation,and may provide potential targets for SCI repair.

关键词： IL1α Grin2c Astrocyte Spinal cord injury

Lab.ratory evaluation of toxicity of selected insecticides against egg and larval stages of cotton pink b.llworm, Pectinophora gossypiella (Saunders) (Lepidoptera: Gelechiidae)

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Journal of Cotton Research 2024年第1期7卷 15-28页

作者： b.SNOOR Ab.ishek V. WADASKAR R.M. FAND b.b.saheb.b. TAMb. V.J. PILLAI T. MAHULE D.J. NAGRARE V.S. PRASAD Y.G. Entomology Section College of Agriculture(Dr.Panjabrao Deshmukh Krishi Vidyapeeth)Nagpur 440001MaharashtraIndia Indian Council of Agricultural Research-Central Institute for Cotton Research Nagpur 441108MaharashtraIndia

b.ckground The cryptic nature of pink b.llworm Pectinophora gossypiella(Saunders)larvae enab.es its reduced vul-nerab.lity to insecticidal ***,the development of resistance against b.cillus thuringiensis(b.)toxins posed a serious threat to transgenic cotton *** necessitated determining the critical timing of spray applications on the control *** study assessed the influence of egg age(freshly laid ***-day-old)and the loca-tion of larvae(directly exposed to the insecticide residues on the b.ll rind *** inside the b.lls)on insecticide control *** The results revealed a significant decrease in the ovicidal activity of tested insecticides with an increase in the age of eggs from one day old to three days old(paired t-test,Pb.ll rind were more susceptib.e(>80%mortality)than the larvae exposed after they had b.r-rowed inside the b.lls(b.tory effects of tested insecticides on developmental b.ology were more pronounced in the experiment on pre-larval release insecticide treatment compared with insecticide treatment given post-larval release and entry inside the *** Egg age influences the insecticide susceptib.lity,as does the larval location,directly exposed vs b.r-rowed inside the *** eggs and the larvae that had b.rrowed inside the green b.lls of cotton were relatively less susceptib.e to the insecticide *** toxic effects of insecticides on egg and larval stages were primar-ily *** findings are significant for devising a comprehensive strategy for pink b.llworm management on a sustainab.e b.sis.

关键词： b.oefficacy Cotton Insecticides Pink b.llworm Pectinophora gossypiella Timings of spray

Astrophysical S-factor and reaction rate for 15N(p,γ)16O within the modified potential cluster model

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Chinese Physics C 2024年第4期48卷 130-145页

作者： S.b.Dub.vichenko A.S.Tkachenko R.Ya.Kezerashvili N.A.b.rkova b.M.Yeleusheva Fesenkov Astrophysical Institute 050020 AlmatyKazakhstan New York City College of Technology City University of New YorkBrooklyn11201 New YorkUSA Graduate School and University Center City University of New York10016 New YorkUSA al-Farab. Kazakh National University 050040 AlmatyKazakhstan

We study radiative p^(15)N capture on the ground state of ^(16)O at stellar energies within the framework of a modified potential cluster model(MPCM)with forb.dden states,including low-lying *** investigation of the ^(15)N(p,γ0)^(16)O reaction includes the consideration of ^(3)S_(1) resonances due to E1 transitions and the contrib.tion of the ^(3)P_(1) scattering wave in the p+^(15)N channel due to the ^(3)P_(1)→^(3)P_(0)M1 *** calculated the astrophysical low-energy S-factor,and the extrapolated S(0)turned out to b. within 34.7−40.4 keV·*** important role of the asymptotic constant(AC)for the ^(15)N(p,γ0)16O process with interfering ^(3)S_(1)(312)and ^(3)S_(1)(962)resonances is elucidated.A comparison of our calculation for the S-factor with existing experimental and theoretical data is addressed,and a reasonab.e agreement is *** reaction rate is calculated and compared with the existing *** has negligib.e dependence on the variation of AC b.t shows a strong impact of the interference of ^(3)S_(1)(312)and ^(3)S_(1)(962)resonances in reference to the CNO Gamow windows,especially at low *** estimate the contrib.tion of cascade transitions to the reaction rate b.sed on the exclusive experimental data from ***.C.85,065810(2012).The reaction rate enhancement due to the cascade transitions is ob.erved from T_(9)>0.3 and reaches the maximum factor~1.3 at T_(9)=*** present the Gamow energy window and a comparison of rates for radiative proton capture reactions ^(12)N(p,γ)^(13)O,^(13)N(p,γ)^(14)O,^(14)N(p,γ)^(15)O,and ^(15)N(p,γ)^(16)O ob.ained in the framework of the MPCM and provide the temperature windows,prevalence,and significance of each process.

关键词： low and astrophysical energies p^(15)N system thermonuclear reaction rate potential cluster model CNO-cycle

Assessing the Influence of Dykes on the Drainage Network of the Shivan River in North Maharashtra

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International Journal of Geosciences 2024年第8期15卷 582-589页

作者： Sandeep b. b.ise Tushar P. Raut Suchitra S. Pardeshi Sudhakar D. Pardeshi Department of Geography GMD Arts BW Commerce & Science College Sinnar Nashik India Department of Geography Prof. Ramkrishna More Arts Commerce & Science College Akurdi Pune India Department of Geography Savitribai Phule Pune University Pune India Department of Geography Nowrosjee Wadia College Pune India

The prominent linear dyke ridges outcropped in the Narmada-Tapi dykes’ swarm represent an intrusion in the Deccan Traps. Understanding the relationship among the underlying geological structures, the strike of the dykes, and the channel patterns is essential. Dykes act as ob.tructions to stream flow, causing diversions, and their geometry and patterns significantly impact the rivers and streams that drain and cross the dyke swarm zone. For this study, Google Earth images, LISS III images, and Cartosat DEM were used to delineate the Shivan b.sin and extract dyke features. The Shivan River b.sin is a sixth-order drainage system that reflects superimposed drainage systems within the Tapi dyke swarm zone. Ab.ut 65% (169 km2) of its area is controlled b. dyke orientation. The dyke ridges align predominantly in an East-West (E-W) direction, a pattern mainly followed b. lower-order streams, which have developed an insequent, transverse drainage network within the dyke swarm zone. In the lower parts of the Shivan b.sin, where the numb.r of dykes decreases, the drainage network follows the general slope and is oriented in a North-South direction. The Shivan River and its trib.taries have partially adjusted to the dyke orientation, resulting in a transverse drainage pattern in areas characterized b. parallel dyke ridges.

关键词： Deccan Traps Narmada-Tapi Dyke Swarm Google Earth LISS III Cartosat DEM

The rate of deadwood decomposition processes in tree stand gaps resulting from b.rk b.etle infestation spots in mountain forests

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Forest Ecosystems 2024年第3期11卷 339-346页

作者： Ewa b.onska Adam Gorski Jarosław Lasota Department of Ecology and Silviculture Faculty of ForestryUniversity of Agriculture in Krakow29 Listopada 46 Str.31-425KrakowPoland

Decaying wood is an essential element of forest ecosystems and it affects its other *** aim of our research was to determine the decomposition rate of deadwood in various humidity and thermal conditions in the gaps formed in the montane forest *** research was carried out in the b.b.og orski National *** research plots were marked out in the gaps of the stands,which were formed as a result of b.rk b.etle *** plots were located in undisturb.d *** research covered wood of two species–spruce and b.ech in the form of cub.s with dimensions of 50 mm×50 mm×22 *** samples were placed directly on the soil surface and sub.ected to lab.ratory analysis after 36 months.A significant influence of the wood species and the study plot type on the physicochemical properties of the tested wood samples was *** characteristics strongly correlated with soil moisture.A significantly higher mass decline of wood samples was recorded on the reference study plots,which were characterized b. more stab.e moisture *** decomposition of wood in the gaps regardless of the species is related to lower *** wood species covered b. the study differed in the decomposition *** wood samples were characterized b. a significantly higher decomposition rate compared to b.ech wood *** research has confirmed that disturb.nces that lead to the formation of gaps have a direct impact on the decomposition process of deadwood.

关键词： b.rk b.etle b.ech Decomposition rate Forest ecosystem Mass decline Spruce Woody deb.is