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Progastrin-releasing peptide and gastrin-releasing peptide receptor mRNA expression in non-tumor tissues of the human gastrointestinal tract

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World journal of Gastroenterology 2006年第16期12卷 2574-2578页

作者： Hans-jürg Monstein Niclas Grahn Mikael Truedsson Bodil Ohisson Molecular Biology Laboratory-LMC University HospitalDepartment of Biomedicine and SurgeryFaculty of Health SciencesS-581 85 LinkpingSweden Department of Clinical Sciences University Hospital MalmS-205 02 MalmSweden

AIM： To investigate the expression of gastrin-releasing peptide （GRP） and GRP-receptor mRNA in non-tumor tissues of the human esophagus, gastrointestinal tract, pancreas and gallbladder using molecular biology techniques. METHODS： Poly A^＋ mRNA was isolated from total RNA extracts using an automated nucleic acid extractor and, subsequently, converted into single-stranded cDNA （sscDNA）. PCR amplifications were carried out using genespecific GRP and GRP-receptor primers. The specificity of the PCR amplicons was further confirmed by Southern blot analyses using gene-specific GRP and GRP-receptor hybridization probes. RESULTS： Expression of GRP and GRP-receptor mRNA was detected at various levels in nearly all segments of the non-tumor specimens analysed, except the gallbladder. In most of the biopsy specimens, coexpression of both GRP and GRP-receptor mRNA appeared to take place. However, expression of GRP mRNA was more prominent than was GRP-receptor mRNA. CONCLUSION： GRP and GRP-receptor mRNAs are expressed throughout the gastrointestinal tract and provides information for the future mapping and determination of its physiological importance in normal and tumor cells.

关键词： Gastrin releasing peptide (GRP) Gastrinreleasing peptide receptor (GRPR) mRNA expression Morphogenesis Gastrointestinal tract

Colon capsule endoscopy: Current status and future directions

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World journal of Gastroenterology 2014年第44期20卷 16596-16602页

作者： Andrea O Tal johannes Vermehren j?rg G Albert Department of Internal Medicine I University Hospital Frankfurt

Colon capsule endoscopy(CCE; Pill Cam Colon; Given Imaging; Yoqneam, Israel) is a minimally invasive wireless technique for the visualization of the colon. With the recent introduction of the second generation colon capsule the diagnostic accuracy of CCE for polyp detection has significantly improved and preliminary data suggest it may be useful to monitor mucosal inflammation in patients with inflammatory bowel disease. Limitations include the inability to take biopsies and the procedural costs. However, given the potentially higher acceptance within an average risk colorectal cancer(CRC) screening population, its usefulness as a screening tool with regard to CRC prevention should be further evaluated.

关键词： Capsule endoscopy Endoscopy Colon capsule endoscopy Colonoscopy Colorectal cancer Colon Colonic capsule endoscopy Inflammatory bowel disease

Expression of the human TSPY gene in the brains of transgenic mice suggests a potential role of this Y chromosome gene in neural functions

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journal of Genetics and Genomics 2011年第5期38卷 181-191页

作者： Tatsuo Kido Stephanie Schubert jrg Schmidtke Yun-Fai Chris Lau Division of Cell and Developmental Genetics Department of MedicineVA Medical Centerand Institute for Human GeneticsUniversity of California Institute of Human Genetics Hannover Medical School

The testis specific protein Y-encoded （TSPY） is a member of TSPY/SET/NAP1 superfamily, encoded within the gonadoblastoma locus on the Y chromosome. TSPY shares a highly conserved SET/NAP-domain responsible for protein--protein interaction among TSPY/SET/NAP 1 proteins. Accumulating data, so far, support the role of TSPYas the gonadoblastoma gene, involved in germ cell tumorigenesis. The X-chromosome homolog of TSPY, TSPX is expressed in various tissues at both fetal and adult stages, including the brain, and is capable of interacting with the multi-domain adapter protein CASK, thereby influencing the synaptic and transcriptional functions and developmental regulation of CASK in the brain and other neural tissues. Similar to TSPX, we demonstrated that TSPY could interact with CASK at its SET/NAP-domain in cultured cells. Transgenic mice harboring a human TSPYgene and flanking sequences showed specific expression of the human TSPYtransgene in both testis and brain. The neural expression pattern of the human TSPYgene overlapped with those of the endogenous mouse Cask and Tspx gene. Similarly with TSPX, TSPY was co-localized with CASK in neuronal axon fibers in the brain, suggesting a potential role（s） of TSPY in development and/or physiology of the nervous system.

关键词： TSPY TSPX CASK Y chromosome Neuron Testis

Carbonates as Foaming Agent in Chip-based Aluminium Foam Precursor

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journal of Materials Science & Technology 2010年第9期26卷 845-850页

作者： Marco Haesche Dirk Lehmhus jrg Weise Manfred Wichmann Irene Cristina Magnabosco Mocellin University of Bremen Fraunhofer IFAM Federal University of Santa Catarina Florianópolis

Replacement of TiH2 as foaming agent by CaCO3（lime） and CaMg（CO3）2（dolomite） for AlMg4.5-and AlSi9Cu3-foams was investigated considering inluences on foaming capability and cellular *** materials were produced from alloy chip and powder mixtures by means of the thixocasting ***9Cu3 variants showed expansion levels suffcient for commercial *** in expansion observed when CaCO3 and CaMg（CO3）2 were compared as foaming agent are explained based on the course of *** performance of dolomite-based foams relies on formation of stabilizing MgO phases,which do not develop during decomposition of CaCO3 in Al-Si-Cu alloys

关键词： Aluminium foam Foaming agent Calcium carbonate Dolomite Stabilisation

SEMS vs cSEMS in duodenal and small bowel obstruction:High risk of migration in the covered stent group

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World journal of Gastroenterology 2013年第37期19卷 6199-6206页

作者： Oliver Waidmann jrg Trojan Mireen Friedrich-Rust Christoph Sarrazin Wolf Otto Bechstein Frank Ulrich Stefan Zeuzem jrg Gerhard Albert Med-izinische Klinik 1 Schwerpunkt Gastroenterologie und Hepatolo-gieKlinikum der Johann Wolfgang Goethe-Universitt Klinik für Allgemein und Viszeralchirurgie Klinikum der johann Wolfgang Goethe-Universitt

AIM:To compare clinical success and complications of uncovered self-expanding metal stents(SEMS)vs covered SEMS(cSEMS)in obstruction of the small ***:Technical success,complications and outcome of endoscopic SEMS or cSEMS placement in tumor related obstruction of the duodenum or jejunum were retrospectively *** primary end points were rates of stent migration and *** end points were the effect of concomitant biliary drainage on migration rate and overall *** data was analyzed according to the Strengthening the Reporting of Observational Studies in Epidemiology ***:Thirty-two SEMS were implanted in 20 *** all patients,endoscopic stent implantation was *** migration was observed in 9 of16 cSEMS(56%)in comparison to 0/16 SEMS(0%)implantations(P=0.002).Stent overgrowth did not significantly differ between the two stent types(SEMS:3/16,19%;cSEMS:2/16,13%).One cSEMS dislodged and had to be recovered from the jejunum by way of *** until migration between SEMS and cSEMS in patients with and without concomitant biliary stents did not significantly differ(HR=1.530,95%CI0.731-6.306;P=0.556).The mean follow-up was 57±71 d(range:1-275 d).CONCLUSION:SEMS and cSEMS placement is safe in small bowel tumor ***,cSEMS is accompanied with a high rate of migration in comparison to uncovered SEMS.

关键词： Endoscopy Digestive system Intestinal neoplasms Self-expandable metal stents Tumor obstruction Self-expandable metal stents complications

Determination of relationships between thermal conductivity and material properties of rocks

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journal of University of Science and Technology Beijing 2004年第4期11卷 297-301页

作者： Kazim Grgülü Mining Engineering Dept. Cumhuriyet University58140-SivasTurkeynergy transfer between the adjacent parts of rocks in underground mines is widely influenced by the thermal conductivity of rocks. The relationships between the thermal conductivity and some material properties of rocks such as the uniaxial compressive strength unit mass tensile strength cohesion Young's modulus point load strength Schmidt rebound hardness Shore scleroscope hardness and toughness strength were investigated. The statistical analysis of the data obtained in laboratory tests shows that the thermal conductivity increases with increasing the uniaxial compressive strength unit mass tensile strength cohesion Young's modulus point load strength Schmidt rebound hardness and Shore scleroscope hardness and decreases with increasing the toughness strength.

Energy transfer between the adjacent parts of rocks in underground mines is widely influenced by the thermal conductivity of rocks. The relationships between the thermal conductivity and some material properties of rocks such as the uniaxial compressive strength, unit mass, tensile strength, cohesion, Young’s modulus, point load strength, Schmidt rebound hardness, Shore scleroscope hardness and toughness strength were investigated. The statistical analysis of the data obtained in laboratory tests shows that the thermal conductivity increases with increasing the uniaxial compressive strength, unit mass, tensile strength, cohesion, Young’s modulus, point load strength, Schmidt rebound hardness and Shore scleroscope hardness, and decreases with increasing the toughness strength.

关键词： thermal conductivity material properties underground mines

Vulnerability of groundwater resources under climate change in the Pannonian basin

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Geo-Spatial Information Science 2019年第4期22卷 345-358,I0010页

作者： Mărgărit-Mircea Nistor National Institute for Economic Research“Costin C.Kiritescu”of the Romanian Academy/Department of Mountain Economy(CE-MONT) Vatra DorneiRomania Department of Hydrogeology Earthresearch CompanyCluj-NapocaRomania

The impact of climate change on groundwater vulnerability has been assessed in the Pannonian basin over 1961–***-resolution climatemodels,aquifers composition,land cover,and digital elevation model were the main factors which served to perform the spatial analysis using Geographical Information *** analysis reported here is focused on the long-term period,including three temporal time sets:the past period of 1961–1990(1990s),the present period of 2011–2040(2020s),and the future period of 2041–2070(2050s).During the 1990s,the high and very high areas of groundwater vulnerability were identified in all the central,western,eastern,southeastern,and northern sides of the Pannonian *** these areas,the water availability is lower and the pollution load index is high,due to the agricultural *** low and very low vulnerability classwas depicted in the South-West part of the basin and in few locations from the peripheral areas,mainly in the North and *** medium groundwater vulnerability spreads over the Pannonian basin,but it ismore concentrated in the central,South,and *** most affected territory is Hungary,while the territories of Slovenia,Croatia,and Bosnia and Herzegovina are less *** the present and future periods,the very high groundwater vulnerability increased in areas by 0.74%and 0.87%,*** low class area decreased between the 1990s and the 2020s by 2.33%and it is expected to decrease up to 2.97%in the *** on this analysis and the groundwater vulnerabilitymaps,the Pannonian basin appears more vulnerable to climate change in the present and *** findings demonstrate that the aquifers from Pannonian basin experience high negative effect under climate *** addition,the land cover contributes to this negative status of groundwater *** original maps of groundwater vulnerability represent an instrument for water management planning and for research.

关键词： Vulnerability aquifers spatial analysis climatic models pollution load index infiltration index water availability

The Role of the Innate Immune System of the Liver in the Control of HBV and HCV

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Virologica Sinica 2008年第2期23卷 116-123页

作者： Ruth Brring jrg F. Schlaak Department of Gastroenterology University Hospital of Essen

Hepatitis B virus (HBV) and Hepatitis C virus (HCV) infection are among the most frequent causes of chronic liver disease worldwide. As recent studies suggested that Toll like receptor (TLR)-based therapies may represent a promising approach in the treatment of HBV infection, we have studied the role of the local innate immune system of the liver as possible mediator of this effect. Murine non-parenchymal liver cells (NPC; Kupffer cells, KC; sinusoidal endothelial cells, LSEC) were isolated from C57/BL6 and stimulated by TLR 1-9 agonists. Supernatants were harvested and assayed for their antiviral activity against HBV in HBV-Met cells and HCV in the murine HCV replicon cell line MH1. Only supernatants from TLR 3 and -4 stimulated KC and TLR 3 stimulated LSEC were able to potently suppress HBV and HCV replication. By using neutralizing antibodies we could demonstrate that the TLR 3-but not the TLR 4 mediated effect is exclusively mediated through IFN-β. Our data indicate that TLR 3 and -4 mediated stimulation of NPC leads to production of IFN-β which can potently suppress HBV and HCV replication. This is of relevance for the local control of viral hepatitis infection by the innate immune system of the liver, the development of novel TLR-based therapeutic approaches and sheds new light on the viral crosstalk between HCV (TLR 3 stimulator) and HBV.

关键词： Hepatitis B Kupffer cells Sinusoidal endothelial cells Interferon

Laser-induced plasma formation in water with up to 400 mj double-pulse LIBS

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Plasma Science and Technology 2024年第1期26卷 93-100页

作者： Marion HENKEL Michelle SIEMENS Ralf METHLING Benjamin EMDE jörg HERMSDORF Steffen FRANKE Diego GONZALEZ Leibniz Institute for Plasma Science and Technology(INP) Felix-Hausdorff-Straße 2Greifswald 17489Germany Laser Zentrum Hannover e.V.(LZH) Hollerithallee 8Hannover 30419Germany

Double-pulse LIBS is a promising technique for deep-sea *** measurements in shallow water with up to 400 mj each pulse were done to select laser parameters which promote optimized spectral line emission from plasma even at elevated pressures,where line broadening until loss of most of the spectral information can *** emission spectroscopy,using a Czerny-Turner spectrometer,has been applied to investigate the dependence of the emitted radiation on laser parameters and hydrostatic *** has been found,that higher laser pulse energies,especially with short pulse delay as required in high water pressure,can also have an adverse effect on the measured spectrum.

关键词： double-pulse LIBS underwater plasma formation emission spectrosocpy