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A reactor system combining reductive dechlorination with co-metabolic oxidation for complete degradation of tetrachloro-entylene

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Journal of Environmental Sciences 2002年第4期14卷 445-450页

作者： TAE HO LEE MICHIHIKO IKE masanori fujita Department of Environmental Engineering Osaka University 2-1 YamadaokaDepartment of Environmental Engineering Osaka University 2-1 YamadaokaDepartment of Environmental Engineering Osaka University 2-1 Yamadaoka Suita Osaka 565-0871 Japan.Suit

A laboratory sequential anaerobic aerobic bioreactor system, which consisted of an anaerobic fixed film reactor and two aerobic chemostats, was set up to degrade tetrachloroethylene (PCE) without accumulating highly toxic degradation intermediates. A soil enrichment culture, which could reductively dechlorinate 900 μM (ca. 150 mg/L) of PCE stoichiometrically into cis 1,2 dichloroethylene ( cis DCE), was attached to ceramic media in the anaerobic fixed film reactor. A phenol degrading strain, Alcaligenes sp. R5, which can efficiently degrade cis DCE by co metabolic oxidation, was used as inoculum for the aerobic chemostats consisted of a transformation reactor and a growth reactor. The anaerobic fixed film bioreactor showed more than 99 % of PCE transformation into cis DCE in the range of influent PCE concentration from 5 μM to 35 μM at hydraulic retention time of 48h. On the other hand, efficient degradation of the resultant cis DCE by strain R5 in the following aerobic system could not be achieved due to oxygen limitation. However, 54% of the maximum cis DCE degradation was obtained when 10 μmol of hydrogen peroxide (H 2O 2) was supplemented to the transformation reactor as an additional oxygen source. Further studies are needed to achieve more efficient co metabolic degradation of cis DCE in the aerobic reactor.

关键词： anaerobic aerobic bioreactor co oxidation reductive dechlorination tetrachloroethylene

Optimal turning method of composting regarding hygienic safety

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Journal of Environmental Sciences 2005年第2期17卷 194-199页

作者： Masafumi Tateda Le Duc Trung Michihiko Ike masanori fujita Department of Environmental Systems Engineering College of TechnologyToyama Prefectural University5180 KurokawaKosugiImizuToyama 939-0398Japan Department of Environmental Engineering Graduate School of EngineeringOsaka University2-1 YamadaokaSuitaOsaka 565-0871Japan

The new turning method was proposed and verified its effectiveness to pathogens by laboratory scale *** the results obtained from the previous studies,it could be said that turning of a composting pile was essential in terms of hygienic aspects but the number of turning should be *** of inactivation was estimated for each composting *** this estimation,turning by layers,which is different from conventional turning that mixes compost pile entirely,was proposed and investigated its performance by *** operations with static pile method,complete mix(conventional)turning method,and proposed turning(layer turning)method were done and their effectiveness on inactivation of indicator microorganism was evaluated and *** results,the conventional turning method was not a proper method in terms of pathogen inactivation,whereas,the proposed turning method showed an excellent performance and should be employed in a composting operation.

关键词： composting turning inactivation hygienic safety

Comparative evaluation of processes for heavy metal removal from municipal solid waste incineration fly ash

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Journal of Environmental Sciences 1998年第4期10卷 75-82页

作者： Masafumi Tateda Michihiko Ike masanori fujita Department of Environmental Engineering Osaka UniversityJapan

Hydrochloric acid leaching, chloride evaporation, acetic acid leaching, and biological leaching were evaluated and compared as processes of heavy metal removal for municipal solid waste incineration fly ash(MSWFA). Six factors, namely, energy consumption, process efficiency, process handling, process cost estimation, cost reduction potential, and study progress, were used in order to find out their advantages and disadvantages and to help develop a better recovery process of heavy metals from MSWFA in terms of treatment of the waste material. Hydrochloric acid leaching process was found to be most balanced among the evaluated processes. It showed superiority on energy consumption, process cost estimation, and study progress. On the other hand, despite of its excellency in process efficiency, chloride evaporation process was most unfavorable mainly due to heavy energy dependence. Biological process, with huge potential of cost reduction, was concluded to be the second best process.

关键词： municipal solid waste incineration fly ash heavy metal removal process process evaluation.

Sorption of biodegradation end products of nonylphenol polyethoxylates onto activated sludge

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Journal of Environmental Sciences 2004年第4期16卷 564-569页

作者： Nguyen Viet Hung Masafumi Tateda Michihiko Ike masanori fujita Shinji Tsunoi Minoru Tanaka Department of Environmental Engineering Graduate School of EngineeringOsaka University 2-1 Yamada-oka Suita Osaka 565-0871 Japan Department of Environmental Systems Technology College of Technology Toyama Prefectural University 5180 Kurokawa Kosugi Imizu Toyama 939-0398 Japan Research Center for Environmental Preservation Osaka University 2-4 Yamada-oka Suita Osaka 565-0871 Japan

Nonylphenol(NP), nonylphenoxy acetic acid(NP1EC), nonylphenol monoethoxy acetic acid(NP2EC), nonylphenol monoethoxylate(NP1EO) and nonylphenol diethoxylate(NP2EO) are biodegradation end products(BEPs) of nonionic surfactant nonylphenolpolyethoxylates (NPnEO). In this research, sorption of these compounds onto model activated sludge was characterized. Sorption equilibrium experiments showed that NP, NP1EO and NP2EO reached equilibrium in about 12 h, while equilibrium of NP1EC and NP2EC were reached earlier, in about 4 h. In sorption isotherm experiments, obtained equilibrium data at 28℃ fitted well to Freundlich sorption model for all investigated compounds. For NP1EC, in addition to Freundlich, equilibrium data also fitted well to Langmuir model. Linear sorption model was also tried, and equilibrium data of all NP, NP1EO, NP2EO and NP2EC except NP1EC fitted well to this model. Calculated Freundlich coefficient(K F) and linear sorption coefficient(K D) showed that sorption capacity of the investigated compounds were in order NP>NP2EO>NP1EO>NP1EC≈NP2EC. For NP, NP1EO and NP2EO, high values of calculated K F and K D indicated an easy uptake of these compounds from aqueous phase onto activated sludge. Whereas, NP1EC and NP2EC with low values of K F and K D absorbed weakly to activated sludge and tended to preferably remain in aqueous phase.

关键词： activated sludge biodegradation end products endocrine disruptor nonylphenol polyethoxylates sorption

Biodegradation of synthetic surfactants by river microorganisms

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Journal of Environmental Sciences 1996年第3期8卷 275-284页

作者： Hideaki Maki Kenrou Tokuhiro Yasuhiro Fujiwara Michihiko Ike Kenji Furukawa masanori fujita(Department of Environmental Engineering, Faculty of Engineering, Osaka University, 2-1 Yamadaoka,Suita, Osaka 565, Japan) Dept. of Environmental Engineering Faculty of Engineering Osaka University 2-1 Yamadaoka Suita Osaka 565 Japan

Four kinds of widely used synthetic surfactants and polyethylene glycol (PEG) which corresponds to the hydrophilic moieties of polyoxyethylene-type nonionic surfactants were subjected to a complete biodegradation test using microorganisms from relatively unpolluted river waters (the TOC-Handai method). Sodium dodecyl sulfate (SDS), alcohol ethoxylate (AE) and PEG were mineralized by most of the microorganisms used as inocula, irrespective of differences in their degradation rates, which is in agreement with their biodegradabilities confirmed so far by other *** degradable surfactants were, however, not completely degraded by some river microorganisms. Among the surfactants tested, SDS was degraded most rapidly by microorganisms of each river in the same manner but the degradation rates of AE and PEG differed considerably according to the inoculum sampling station. Sodium linear alkylbenzensulfonate (LAS) and nonylphenol ethoxylate (NPE). which are known to be recalcitrant molecules, were not degraded completely by microorganisms of any of the river waters within the testing period; degradation of the former rarely proceeded , while that of the latter ceased leaving the well-known intermediates. The residual metabolites of NPE generated by biodegradation, which were analyzed by high-performance liquid chromatography and gas chromatography/mass spectrometry, revealed that this surfactant was transformed to carboxylated metabolites, nonylphenoxy acetic acid (NP1EC)and [(nonylphenoxy)ethoxy] acetic acid (NP2EC) exclusively.

关键词： biodegradation surfactants river microorganisms TOC

Low-Molecular-Weight Heparin and Protamine-Based Polyelectrolyte Nano Complexes for Protein Delivery (A Review Articles)

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Journal of Biomaterials and Nanobiotechnology 2011年第5期2卷 500-509页

作者： Masayuki Ishihara Satoko Kishimoto Megumi Takikawa Yasutaka Mori Shingo Nakamura masanori fujita 不详

We produced low-molecular-weight heparin/protamine micro (nano) particles (LMW-H/P MPs·NPs) as a carrier for heparin-binding growth factors (GFs), such as fibroblast growth factor (FGF)-2 and various GFs in platelet-rich plasma (PRP). A mixture of LMW-H (MW: approximately 5000 Da, 6.4 mg/ml) and protamine (MW: approximately 3000 Da, 10 mg/ml) at a ratio of 7:3 (vol:vol) yields a dispersion of micro (nano) particles (200 nm - 3 μm in diameter). The diluted LMW-H solution in saline (0.32 mg/ml) mixed with diluted protamine (0.5 mg/ml) at a ratio at 7:3 (vol:vol) resulted in soluble nanoparticles (approximately 100 nm in diameter). The generated NPs could be then stabilized by adding 2 mg/ml dextran (MW: 178-217 kDa) and remained soluble after lyophilization of dialyzed LMW-H /P NPs solution. The LMW-H/P MPs·NPs adsorb GFs, control their release, protect GFs and activate their biological activities. Furthermore, administration of GFs-containing F/P MPs·NPs exhibited significantly higher inductions of vascularization and fibrous tissue formation in vivo than GFs alone. LMW-H/P MPs·NPs can also efficiently bind to tissue culture plates and retain the binding of GFs. The LMW-H/P MPs·NP-coated matrix with various GFs or cytokines provided novel biomaterials that could control cellular activity such as proliferation and differentiation. Thus, LMW-H/P MPs·NPs are an excellent carrier for GFs and are a functional coating matrix for various kinds of cell cultures.

关键词： Polyelectrolyte Complexes Nanoparticles Heparin-Binding Growth Factors Platelet-Rich Plasma Drug Delivery

MicroRNA-329-3p inhibits the Wnt/β-catenin pathway and proliferation of osteosarcoma cells by targeting transcription factor 7-like 1

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Oncology Research 2024年第3期32卷 463-476页

作者： Hur SUN masanori KAWANO TATSUYA IWASAKI ICHRO ITONAGA YUTA KUBOTA HROSHI TSUMURA KAZUHRO TANAKA Department of Orthopaedic Surgery Faculty of MedicineOita UniversityOita879-5503Japan

An important factor in the emergence and progre sion of osteosarcoma(OS)is the dysregulated expression of microRNAs(miRNAs).Transcription factor 7-like 1(TCF7LI),a member of the T cell factor/lymphoid enhancer factor(TCF/LEF)transcription factor family,interacts with the Wnt signaling pathway regulator β-catenin and acts as a DNA-specific binding *** study sought to elucidate the impact of the interaction between miR 3293p and TCF7L1 *** growth and apoptosis of OS and analyze the regulatory expression relationship between miRNA and mRNA in osteosarcoma cells using a variety of ***329-3p was significantly downregulated,while TCF7L1 was considerably up-regulated in all examined OS cell ***,a clinical comparison study was performed using the TCGA ***,the regulatory relationship between miR-329-3p and TCF7L1 on the proliferation and apoptosis of OS cells was verified through in vitro and in vivo *** miR 329-3p was transfected into the OS cell line,the expression of TCF7L1 decreased,the proliferation of OS cells was inhibited,the cytoskeleton disintegrated,and the nucleus condensed to fom apoptotic *** expression of proteins that indicate apoptosis increased *** cell cycle was arrested in the G0/G1 phase,and the G1/S transition was *** introduction of miR 3293p also inhibited downstream Cyclin D1 of the Wnt *** experiments indicated that the overexpression of miR-329-3p signi ficanly inhibited the growth of OS xenografts in nude mice,and the expression of TCF7L1 and C-Myc in tumor tssues *** 329-3p was significantly reduced in OS cells and played a suppressive role in tumorigenesis and proliferation by targeting TCF7L1 both in vitro and in *** cell cycle arrest and pathway inhibition were observed upon the regulation of TCF7LI by miR *** these results,it can be inferred that miR.3293p exerts anticancer efects in osteosarcom

关键词： MiR-329-3p TCF7L1 Wnt/β-catenin pathway Osteosarcoma Proliferation

Correction: MicroRNA-329-3p inhibits the Wnt/β-catenin pathway and proliferation of osteosarcoma cells by targeting transcription factor 7-like 1

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Oncology Research 2024年第8期32卷 1369-1370页

作者： HUI SUN masanori KAWANO TATSUYA IWASAKI ICHIRO ITONAGA YUTA KUBOTA HIROSHI TSUMURA KAZUHIRO TANAKA Department of Orthopaedic Surgery Faculty of MedicineOita UniversityOita879-5503Japan

In the article‘MicroRNA-329-3p inhibits the Wnt/β-catenin pathway and proliferation of osteosarcoma cells by targeting transcription factor 7-like 1’(Oncology Research,2024,Vol.32,No.3,pp.463−***:10.32604/or.2023.044085),there was an error in the compilation of *** have revised Fig.8D to correct this error.A corrected version of Fig.8 is *** correction does not change any results or conclusions of the *** apologize for any inconvenience caused.

关键词： osteosarcoma correction revised

Hand Cooling Enhances the Proprioceptive Drift during Rubber Hand Illusion

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Journal of Behavioral and Brain Science 2024年第7期14卷 210-226页

作者： masanori Sakamoto Yuta Akaike Kazuya Tatsumi Hirotoshi Ifuku Division of Health and Physical Education Graduate School of Education Kumamoto University Kumamoto City Japan Lifelong Sports and Welfare Course Faculty of Education Kumamoto University Kumamoto City Japan

Background: The neural representation of the body is easily altered by integrating multiple sensory signals in the brain. The “Rubber Hand Illusion” (RHI) is one of the most popular experimental paradigms to investigate this phenomenon. During this illusion, ownership of a rubber hand is temporarily induced. It was shown that external and continuous cooling of the palm enhanced the RHI, suggesting an association between altered the autonomic nervous system regulation and altered the sense of ownership of a specific limb. Purpose: To investigate whether artificially cooling the entire hand for a short period affects the magnitude of the illusion. Methods: Participants immersed their entire hand in cool, cold, or warm water for 1 min before the RHI procedure. Results: We found that cooling the entire hand enhanced the proprioceptive drift during the RHI but not the subjective feeling of ownership. In contrast, warming and intense cooling of the entire hand did not affect the RHI strength. Conclusion: Our results suggest that transient and moderate cooling of the entire hand was sufficient in enhancing the illusory disembodiment of one’s own hand.

关键词： Hand Temperature Cooling Rubber Hand Illusion Ownership Proprioceptive Drift