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Study of phylogenic relationships of some wild and crop species of Iranian Crocus by ITS nuclear loci

BIOCELL 2019年第3期43卷 225-232页

作者： FARSHID GOLSHANI BARAT ALI FAKHERI MAHMOOD SOLOUKI NAFISEH MAHDINEZHAD majid reza kiani feriz Department of Plant Breeding and Biotechnology Faculty of AgricultureUniversity of ZabolZabolIran Department Khorasan Razavi Agricultural and Natural Resources Research and Education CenterAREEOMashhadIran

ITS(Internal Transcription Spacer of nuclear ribosomal DNA)regions method was applied to identify and study the phylogenetic relationships existing between 41 individuals of Crocus,including 8 species collected in Iran(4 crop and 8 wild individuals)and 16 species(2 crop and 27 wild individuals)already registered in GenBank(NCBI).PCR amplifications were performed using the primer designed on the nucleotide sequence of nuclear region(ITS).A total of 12 sequences were obtained and registered in NCBI *** particular,4 of these sequences were not already present in the scientific *** polymorphic sites were counted for ITS(N=97).The results showed that,the Cytosine base is the most abundant *** high conservation was observed in the ITS regions of the populations(0.761).Each sample could be distinguished from the others in the phylogenic trees developed based on the data obtained by ITS barcode *** general,ITS sequence indicating high resolution at the species level is very suitable for phylogenetic studies in the Crocus *** analysis demonstrated the genetic relationship between crop saffron and wild Crocus *** to the results of this study,the wild species of *** and *** were considered as the closest relatives of the *** results of the study also indicated that,the different ecotypes of *** may have evolved through independent events probably due to the geographic and environmental pressures.

关键词： Crocus Haplotype ITS Phylogeny Saffron

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