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Cloning and characterization of 66 kDa streptavidin-binding peptides(SBP)of Pisum sativum *** specific to ***

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BIOCELL 2019年第3期43卷 155-166页

作者： mahmoud moustafa SAAD ALAMRI TAREK TAHA ALI SHATI SULAIMAN ALRUMMAN MOHAMED ALKAHTANI Department of Biology College of ScienceKing Khalid University9004AbhaKingdom of Saudi Arabia Department of Botany Faculty of ScienceSouth Valley UniversityQenaEgypt Environmental Biotechnology Department Genetic Engineering and Biotechnology Research Institute(GEBRI)City of Scientific Research and Technological Applications(SRTA-City)New Borg El-Arab City21934AlexandriaEgypt

The aim of the current research was to clone and to characterize the partial 66 kDa streptavidin-binding peptide(SBP)found in the germinated embryos of Pisum sativum *** pea(*** ***)embryos possess prominent 66 kDa SBPs that gradually disappeared after few hours of germination in germinated embryos,but not in the *** total RNA was isolated from embryos of *** but could not be isolated from the *** partial nucleotides sequences of 66 kDa SBPs of embryonic stalk(*** ***)were cloned and identified using pMOSBlue vector.66 kDa(SBP)gene from the embryos of *** *** possesses 327 bp having an open reading frame(ORF)region in a part of the gene that encoded for 108 amino *** showed similarity among 66 kDa SBPs *** ***,with *** seed biotinylated protein(SBP65)and *** sbp65a mRNA with DNA distance matrix between 0.0094 to ***-TOF mass spectrometry analysis of 66 kDa(SBP)proteins showed it had similar short peptides to 19 proteins found in different organisms,especially Convicilin precursor,and the seed biotinylated protein in *** alignment results of both nucleotide sequences and amino acid residues either from cloning or MALDI-TOF-MS showed differences with related species,especially *** mRNA was found in the cotyledons during seeds germination,which means no metabolic activities and this part may act only as food reservoirs for growing newly embryos.

关键词： Cloning Embryo 66 kDa Pisum sativum Streptavidin-binding peptides(SBP)

Fungal assembly of L-asparaginase using solid-state fermentation:a review

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BIOCELL 2020年第2期44卷 147-155页

作者： SALLY NASER WESAMELDIN SABER MOHAMMAD EL-METWALLY mahmoud moustafa ATTALLA EL-KOTT Life Sciences Department College of Science and Literature Mahyel AseerKing Khalid UniversityAbhaSaudi Arabia Unit of Food Bacteriology Central Laboratory of Food HygieneMinistry of HealthBranch in ZagazigSharkiaEgypt Microbial Activity Unit Department of MicrobiologySoilsWater and Environment Research InstituteAgricultural Research CenterGizaEgypt Botany and Microbiology Department Faculty of ScienceDamanhour UniversityEl-BeheraEgypt Biology Department College of ScienceKing Khalid UniversityAbha9004Saudi Arabia Botany and Microbiology Department Faculty of ScienceSouth Valley UniversityQenaEgypt Zoology Department Faculty of ScienceDamanhour UniversityEl-BeheraEgypt

Because of its antitumor therapeutic-activity,as well as its application in food industries to improve the quality,L-asparaginase has attracted considerable attention from several *** recent years,fungi have occupied advanced rank among microorganisms in the production process of the *** review is spotting the light on the advantages of fungal enzyme and its applications in the food industry and *** solid-state fermentation was discussed as the wide alternative and most accepted biosynthesis ***,some lights were also spotted to the statistical experimental design of the fermentation process,mainly on the methodology of the response surface for L-asparaginase biosynthesis by ***,the immobilization of the enzyme and the features of the widely used solid substrates for the maximization of the production process were explored.

关键词： Immobilization Statistical designs Response surface methodology Optimization

Antimicrobial activities of three seaweeds extract against some human viral and bacterial pathogens

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BIOCELL 2022年第1期46卷 247-261页

作者： JELAN MOFEED MOHAMED DEYAB ABDELNASER MOHAMED mahmoud moustafa SALLY NEGM EMAD EL-BILAWY Faculty of Science King Salman International UniversitySinaiEgypt Faculty of Fish Resources Suez UniversitySuezEgypt Faculty of Science Damietta UniversityDamiettaEgypt Department of Biology College of ScienceKing Khalid UniversityAbha9004Saudi Arabia Department of Botany and Microbiology Faculty of ScienceSouth Valley UniversityQenaEgypt Life Sciences Department Faculty of Science and ArtKing Khalid UniversityMahayel Aseer 61421Saudi Arabia Unit of Food Bacteriology Central Laboratory of Food HygieneMinistry of HealthSharkia44516Egypt

Microbial infections cause complicated health influences along with bad economic *** the present investigation,three dominant seaweeds namely,Amphiroa anceps,Corallina officinalis and Sargassum filipendula were collected from different Egyptian sites at the Red Sea and Mediterranean Sea during autumn *** extracts of the three algae were screened for their antibacterial activity against three pathogenic bacteria Salmonella typhiimurium,Staphylococcus aureus and Escherichia coli,in addition to in vitro antiviral activity against Rotavirus(RV),and Coxsackie virus B3(CVB3)that cause severe diseases in *** extract of ***,*** and *** inhibit *** cells by 57.1%,85.7%,and 91.4%,*** highest level of concentration of the *** extract(100μg/mL)inhibits 100%of Staphylococcus aureus cells followed by *** and *** extract which inhibit 82.5%and 75%of ***,the highest concentration of *** extract inhibits *** by 80%.The extract of *** exhibited a high antiviral effect against RV infection with TI=22 and virus titers lessened by 2.75 log TCID_(50) followed by extractions of *** with TI=18.3 and virus titers reduced by 2.5 log TCID_(50).Against CVB3 infection,the extract of *** causes the highest antiviral activity with TI=15 and reduce the viral titers by 2.5 log TCID_(50),followed by extractions of *** with TI=8.8 and inhibition of virus titers by 1.75 log TCID_(50).Extract of *** displayed the lowest antiviral effects against RV and CVB3 infection with TI=2.4 and 1.4,*** obtained results clarified that the extract of three marine seaweeds maintains a potent antimicrobial activity,making them a future promising source of new antimicrobial drugs.

关键词： Seaweeds Antimicrobial Antibacterial Cytotoxicity Antiviral

Potential detoxification of aflatoxin B2 using Kluyveromyces lactis and Saccharomyces cerevisiae integrated nanofibers

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BIOCELL 2017年第2期41卷 67-73页

作者： mahmoud moustafa TAREK TAHA MOHAMED ELNOUBY NEHAL EL-DEEB GAMAL HAMAD M.A.ABUSAIED SULAIMAN ALRUMMAN Department of Biology College of ScienceKing Khalid University9004AbhaKingdom of Saudi Arabia(KSA) Botany Department Faculty of ScienceSouth Valley UniversityQenaEgypt Environmental Biotechnology Department Genetic Engineering and Biotechnology Research Institute(GEBRI)City of Scientific Research and Technological Applications(SRTA-City)New Borg El-Arab City21934AlexandriaEgypt Composite and Nanostructured Materials Research Department Advanced Technology and New Materials Research InstituteCity of Scientific Research and Technological Applications(SRTA-City)New Borg El-Arab City21934AlexandriaEgypt Department of Biopharmaceutical Product Research Genetic Engineering and Biotechnology Research Institute(GEBRI)City of Scientific Research and Technological Applications(SRTA-City)New Borg El-Arab City21934AlexandriaEgypt Food Technology Department Arid Land Cultivation Research Institute(ALCRI)City of Scientific Research and Technological Applications(SRTA-City)New Borg El-Arab City21934AlexandriaEgypt Polymer Materials Research Department Advanced Technology and New Materials Research InstituteCity of Scientific Research and Technological Applications(SRTA-City)New Borg El-Arab City21934AlexandriaEgypt

Current investigation has shown that human exposure to aflatoxins is not limited to the administration of contaminated cereals,but water is another possible *** study was aimed to design easily applicable method to eliminate aflatoxin B2(AFB2)from contaminated drinking *** has been used for preparation of probiotic-coated polyvinyl alcohol(PVA)and cellulose acetate(CA)*** of these hybrid nanofibers were studied by scanning electron microscopy(SEM)and Fourier-transformed infrared spectroscopy(FT-IR).SEM showed the proper coating of probiotic strains(Kluyveromyces lactis CBS 2359 and Saccharomyces cerevisiae ATCC 9763)on both nanofiber *** areas(1-5 cm^(2))of the probiotic-nanofiber hybrid were used to enhance the removal of 20 ng/ml of aflatoxin B2(AFB2)from prepared AFB2-contaminated water over *** revealed that a 5 cm^(2) area of probiotic-coated PVA nanofibers can eliminate 97.5% of AFB2 as compared to 87.5%,90.5%,93.5%,and 95.5%,for 1 cm2,2 cm^(2),3 cm^(2),and 4 cm^(2),respectively,while probiotic-coated CA nanofibers were slightly less ***,the cytotoxicity of probiotics-CA treated water on cultured human fibroblasts was almost 10 times lower than the cytotoxicity recorded in probiotics-PVA treated ***,results of the current research suggest that probiotics-polymer nanofiber membranes can be used as an extra stage in the water purification system for the treatment of AFB2-contaminated water.

关键词： Nanofibers Aflatoxin B2 Cytotoxicity Water treatment

Nanoparticles induce the biosynthesis and activity of the new possible therapeutic proteinase source,Talaromyces purpureogenus KJ584844

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BIOCELL 2021年第1期45卷 119-127页

作者： SALLY NEGM MOHAMMAD EL-METWALLY WESAM ELDIN SABER SAHAR ABO-NEIMA mahmoud moustafa ATTALLA EL-KOTT Life Sciences Department College of Science and literature Mahyel AseerKing Khalid UniversityAbhaSaudi Arabia Unit of Food Bacteriology Central Laboratory of Food HygieneMinistry of HealthSharkiaEgypt Department of Botany and Microbiology Faculty of ScienceDamanhour UniversityEl-BeheraEgypt Microbial Activity Unit Department of MicrobiologySoilsWater and Environment Research InstituteAgricultural Research CenterGizaEgypt Physics Department Faculty of ScienceDamanhour UniversityDamanhourEgypt Department of Biology College of ScienceKing Khalid University9004AbhaSaudi Arabia Department of Botany and Microbiology Faculty of ScienceSouth Valley UniversityQenaEgypt Zoology Department Faculty of ScienceDamanhour UniversityEl-BeheraEgypt

The need for the bacterial proteinase is rapidly growing,urging to catch a lowcost medium for the microbial fermentation,nanoparticles can play a vital role in this *** proteinase of Talaromyces purpureogenus was produced on the tubers of Helianthus tuberosus that also operated as solid support for the fermentation *** interface amongst nitrogen sources(NH_(4)Cl and yeast extract)was investigated,applying the statistical modeling of central composite design under solid-state *** optimum medium for proteinase secretion was stimulated by 979.82 mg NH_(4)Cl and 437.68 mg yeast extract per 100 g substrate,yielding 108.15 U/g *** Plackett-Burman experimental design,the nanoparticles Co,Ni and Fe were assessed as inducers for proteinase *** nanoparticles(5 ppm)were the greatest in both proteinase production by the fungus as well as an inducer of the proteolysis process by the enzyme when using faba bean straw as a proteinaceous substrate in the reaction mixture,liberating the extreme quantity of amino acids,compared with the lack of the *** findings suggest the incorporation of Co nanoparticles in both the proteinase fabrication process and during the degradation of proteinaceous materials induce proteinase *** approach could be extended to modulate the productivity and activity of similar biomolecules.

关键词： Experimental design Helianthus tuberosus proteolysis solid-state fermentation

Gene expression of 49 kDa apyrase,cytoskeletal proteins,ATPase,ADPase and amino acid contents of Pisum sativum(L.)cells germinated in Euryops arabicus(*** Jaub.&Spach)water extract

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BIOCELL 2019年第2期43卷 81-87页

作者： mahmoud moustafa SAAD ALAMRI HOIDA ZAKI NAGLAA LOUTFY TAREK TAHA ALI SHATI MOHAMED ALKAHTANI SAJDA SIDDIQI Department of Biology College of ScienceKing Khalid University9004AbhaKingdom of Saudi Arabia(KSA) Department of Botany Faculty of ScienceSouth Valley UniversityQenaEgypt Environmental Biotechnology Department Genetic Engineering and Biotechnology Research Institute(GEBRI)City of Scientific Research and Technological Applications(SRTA-City)New Borg El-Arab City21934AlexandriaEgypt

The present research reports of quick and marked changes induced by plant extract of Euryops arabicus in the gene expression of 49-kDa apyrases,cytoskeletal proteins,ATPases,ADPase and amount of amino acid of pea(Pisum sativum ***).Pellets of cytoskeletals proteins(27000 xg)were probed with anti-apyrase antibody,biotinylated anti-rat,actin and alpha and beta-tubulin for Western *** and ADPase activities were determined based on the hydrolytic efficacy of adenine triphosphate and adenine *** 72 hours,the abundance of apyrases,cytoskeletal proteins and amount of amino acid in pellets of 27000 xg of germinated pea seeds in *** extracts were sharply increased than those sown in distilled *** the samples exhibited that the stems had more amount from apyrases,cytoskeletal proteins,amino acids and ATPase and ADPase activities than primary leaves and primary roots that were germinated either on *** water extract or in distilled *** on the enzyme’s capability to hydrolyse nucleotide triphosphate and nucleotide diphosphate as well as the direct association between expression of 49-kDa apyrase and cytoskeletal proteins,*** water extract had an important effect on plant germinations.

关键词： Cytoskeletal proteins ATPase ADPase Pisum sativum Euryops arabicus

Isolation, purification and structure elucidation of three newbioactive secondary metabolites from Streptomyces lividans AM

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BIOCELL 2021年第5期45卷 1263-1271页

作者： MOHAMMAD EL-METWALLY MAMDOUH ABDEL-MOGIB MANAL ELFEDAWY GAAD SOHSAH AHMED REZK mahmoud moustafa MOHAMED SHAABAN Botany and Microbiology Department Faculty of ScienceDamanhour UniversityDamanhour22511Egypt Chemistry Department Faculty of ScienceMansoura UniversityEl-Mansoura35516Egypt Bioprocess Development Department Genetic Engineering and Biotechnology Research InstituteCity of Scientific Research and Technological Applications(SRTA-City)New Borg El-Arab CityAlexandria21934Egypt Biology Department College of ScienceKing Khalid UniversityAbha9004Saudi Arabia Chemistry of Natural Compounds Department Division of Pharmaceutical IndustriesNational Research CentreDokki-Cairo12622Egypt Organic and Bioorganic Chemistry Department of ChemistryBielefeld UniversityBielefeld33615Germany

Microorganisms are a huge mine of bioactive metabolites,and actinomycetes are one of the very active groups in this *** this article,we are concerned about the full taxonomical characterization of Streptomyces lividans AM,isolated from Egyptian *** isolate produced three new bioactive metabolites,namely:1-Nona-decanoyl,4-oleyl disuccinate(1),filoboletic acid;(9Z,11E)-8,13-dihydroxy octadeca-9,11-dienoic acid(2),and sitosteryl-3β-D-glucoside(3).Extensive 1D and 2D NMR and HR-mass spectrometry were used to elucidate the structures of the three ***,ten known compounds were also *** antimicrobial activity of the producing organism and newly reported compounds(1–3)was investigated against a selected group of pathogenic microorganisms.A full taxonomical characterization of the strain was described as well.

关键词： Bioactive metabolites Streptomyces sp. Taxonomy Biological activity

Feasible design for electricity generation from Chlorella vulgaris using convenient photosynthetic conditions

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BIOCELL 2018年第1期42卷 7-11页

作者： mahmoud moustafa TAREK TAHA MOHAMED ELNOUBY M.A.ABU-SAIED ALI SHATI MOHAMED AL-KAHTANI SULAIMAN ALRUMMAN Department of Biology College of ScienceKing Khalid University9004AbhaKingdom of Saudi Arabia(KSA) Department of Botany Faculty of ScienceSouth Valley UniversityQenaEgypt Environmental Biotechnology Department Genetic Engineering and Biotechnology Research Institute(GEBRI)City of Scientific Research and Technological Applications(SRTA-City)New Borg El-Arab City21934AlexandriaEgypt Composite and Nanostructured materials research department Advanced Technology and New Materials Research InstituteCity of Scientific Research and Technological Applications(SRTA-City)New Borg El-Arab City21934AlexandriaEgypt Polymer Materials Research Department Advanced Technology and New Materials Research InstituteCity of Scientific Research and Technological Applications(SRTA-City)New Borg El-Arab City21934AlexandriaEgypt

Many recent studies are concerned with low cost,easy to handle and alternative renewable energy as a feasible solution for the upcoming crisis of energy *** are unicellular entities the can only depend on CO_(2),water and solar power to cover their nutritional *** current study is concerned with using algal cells in a polymeric hydrogel,as a cheap source of energy for electricity *** vulgaris has been proved to be a promising algal species for electricity generation,as compared with Micractinium *** hydrogel has been used for the immobilization of both algal species in order to protect them from the adverse surrounding conditions in addition to its ability to slowly release the required water molecules according to *** these conditions,*** showed the ability to generate 60 mV compared with 15 mV generated by *** electron micrographs showed nano-threads that bind the *** cells to each other,indicating the ability of algae to create nanowires that facilitate the electron transfer among algal cells and from cells to the nearest ***,we would expect an increase in the produced potential with simultaneous amendment of environmentally polluted water,such as sewage or waste *** of FTIR and raman spectroscopy proved the presence of the characteristic groups of PVA hydrogel and proved the proper integration of the algal cells inside the hydrogel cavities.

关键词： Chlorella vulgaris immobilization PVA hydrogel Electricity generation Algae Microbial fuel cell(MFC)

Secondary antiviral metabolites from fungi with special reference to coronaviruses

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BIOCELL 2022年第8期46卷 1979-1988页

作者： MOHAMED SALEM MOHAMMAD EL-METWALLY WESAMELDIN SABER SALLY NEGM ATTALLA EL-KOTT YASSER MAZROUA ABEER MAKHLOUF mahmoud moustafa Department of Chemistry Faculty of Science and ArtKing Khalid UniversityMohailAssirSaudi Arabia Department of Chemistry Faculty of ScienceAl-Azhar UniversityCairoEgypt Botany and Microbiology Department Faculty of ScienceDamanhour UniversityEl-BeheraEgypt Microbial Activity Unit Department of MicrobiologySoilsWater and Environment Research InstituteAgricultural Research CenterGizaEgypt Life Sciences Department College of Science and ArtKing Khalid UniversityMohailAseerSaudi Arabia Unit of Food Bacteriology Central Laboratory of Food HygieneMinistry of HealthSharkiaEgypt Department of Biology College of ScienceKing Khalid UniversityAbhaSaudi Arabia Zoology Department Faculty of ScienceDamanhour UniversityEl-BeheraEgypt Department of Business Administration Community CollegeKing Khalid UniversityAbhaSaudi Arabia Department of Agricultural Economics Faculty of AgricultureTanta UniversityTantaEgypt Department of Botany Faculty of AgricultureMenoufia UniversitySheben El-KomMenoufiaEgypt Botany and Microbiology Department Faculty of ScienceSouth Valley UniversityQenaEgypt

Profound inspection of the life forms on the earth teaches how to be the complexity of interrelationships among the various *** of the emergence of novel viruses all the time and the inadequate of vaccines and antivirals,viral contagions are amongst the most causative diseases affecting people *** exemplify a massive source of bioactive molecules as,many fungal secondary metabolities like Oxoglyantrypine,Carneic acid F,Scedapin C,Asteltoxin E,Phomanolide,Norquinadoline A and Quinadoline B have antiviral *** review deals with how secondary metabolites of fungi can help in the war against viruses in general and especially Coronaviruses moreover several pieces of literature pointed out that many clusters of fungi in different biotopes are waiting to be exploited.

关键词： COVID-19 SARS-CoV-2 Antibiotics Virus Fungi