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Establishment of a Multiplex Detection Method for Common Bacteria in Blood Based on Human mannan-Binding Lectin Protein-Conjugated magnetic Bead Enrichment Combined with Recombinase-Aided PCR Technology

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Biomedical and Environmental Sciences 2024年第4期37卷 387-398页

作者： ZHAO Zi Jin CHEN Xiao Ping HUA Shao Wei LI Feng Yu ZHAO Meng XING Chen Hao WANG Jie TIAN Feng Yu ZHANG Rui Qing LYU Xiao Na HAN Zhi Qiang WANG Yu Xin LI Hong Yi SHEN Xin Xin ma xue jun TIE Yan Qing Graduate School Hebei North UniversityZhangjiakou 075000HebeiChina Department of Clinical Laboratory Hebei General HospitalShijiazhuang 050051HebeiChina National Key Laboratory of Intelligent Tracking and Forecasting for Infectious Diseases NHC Key Laboratory of Medical Virology and Viral DiseasesNational Institute for Viral Disease Control and PreventionChinese Center for Disease Control and PreventionBeijing 102206China National Institute for Communicable Disease Control and Prevention Chinese Center for Disease Control and PreventionBeijing 102206China Department of Clinical Laboratory Pathology Diagnostic Center Shiyan General HospitalShiyan 442000HubeiChina Graduate School Hebei Medical University Shijiazhuang 050031HebeiChina

Objective Recombinase-aided polymerase chain reaction(RAP)is a sensitive,single-tube,two-stage nucleic acid amplification *** study aimed to develop an assay that can be used for the early diagnosis of three types of bacteremia caused by Staphylococcus aureus(SA),Pseudomonas aeruginosa(PA),and Acinetobacter baumannii(AB)in the bloodstream based on recombinant human mannanbinding lectin protein(M1 protein)-conjugated magnetic bead(M1 bead)enrichment of pathogens combined with *** Recombinant plasmids were used to evaluate the assay *** blood influenza bacteria were used for the specific *** and clinical plasma samples were enriched with M1 beads and then subjected to multiple recombinase-aided PCR(M-RAP)and quantitative PCR(qPCR)*** analysis was used to evaluate the consistency between the two *** The M-RAP method had sensitivity rates of 1,10,and 1 copies/μL for the detection of SA,PA,and AB plasmids,respectively,without cross-reaction to other bacterial *** M-RAP assay obtained results for<10 CFU/mL pathogens in the blood within 4 h,with higher sensitivity than qPCR.M-RAP and qPCR for SA,PA,and AB yielded Kappa values of 0.839,0.815,and 0.856,respectively(P<0.05).Conclusion An M-RAP assay for SA,PA,and AB in blood samples utilizing M1 bead enrichment has been developed and can be potentially used for the early detection of bacteremia.

关键词： Staphylococcus aureus Pseudomonas aeruginosa Acinetobacter baumannii Human mannan-binding lectin protein Bloodstream infection Recombinase-aided PCR assay Multiple detection

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人工智能赋能老年教育:角色、挑战与路径

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广州开放大学学报 2024年第1期24卷 1-7,107页

作者：马学俊程维维宁波职业技术学院建筑与艺术学院浙江宁波315800 浙江纺织服装职业技术学院马克思主义学院浙江宁波315211

人工智能以其强大而又独特的优势,逐步扮演起变革者、管理者和评价者的角色,并与老年教育初步融合,探索形成了老年教育学习网、老年大学5G智慧校园、智慧养老社区等发展模式。然而,人工智能宛如达摩克利斯之剑,也使老年教育面临诸多风... 详细信息

人工智能以其强大而又独特的优势,逐步扮演起变革者、管理者和评价者的角色,并与老年教育初步融合,探索形成了老年教育学习网、老年大学5G智慧校园、智慧养老社区等发展模式。然而,人工智能宛如达摩克利斯之剑,也使老年教育面临诸多风险挑战:“唯技术主义”引发老年教育价值取向偏差位移;“信息茧房”导致老年教育内容汲取同质单一;“智能鸿沟”致使老年教育质量效果降低削弱;“机制缺位”制约老年教育发展布局长久有效。人工智能赋能老年教育是一项战略性、长期性、系统性的综合工程,理应通过优化智能技术、加大资源供给、提升智能素养、构建统筹机制等路径释放叠加效应,增强老年人的获得感、成就感和满足感。

关键词：人工智能老年教育老年人风险挑战

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Detection and Identification of Six Foodborne Bacteria by Two-tube Multiplex Real Time PCR and Melting Curve Analysis

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Biomedical and Environmental Sciences 2014年第10期27卷 770-778页

作者： NIU Pei Hua ZHANG Chen WANG Ji TAN Wen Jie ma xue jun Key Laboratory for Medical Virology Ministry of Health National Institute for Viral Disease Control and Prevention Chinese Center for Disease Control and Prevention

Objective This study is aimed to develop a two-tube melting curve-based multiplex real time PCR assay （MCMRT-PCR） for the simultaneous detection of six common foodborne pathogenic bacteria （diarrhoeagenic Escherichia coli, Salmonella, and 5higella in tube 1, Staphylococcus aureus, Vibrio parahaemolyticus, and Listeria monocytogenes in tube 2）. Methods A two-tube MCMRT-PCR assay was performed on 7900HT Fast Real-Time PCR System {Applied Biosystems, USA）. Amplification by PCR was optimized to obtain high efficiency. The sensitivity and specificity of assays were investigated. Results The detection limit of optimized MCMRT-PCR assay was 3.9x102 CFU/mLfor S. aureus, 4.4x102 CFU/mL for L. monocytogenes, 3.0x102 CFU/mL for Salmonella, 2.5x102 CFU/mL for Shigella, 2.1x102 CFU/mL for V. parahaemolyticus, and 1.2x102 CFU/mL for E. coll. The feasibility of MCMRT-PCR was further evaluated using artificially contaminated milk, the sensitivity was at the level of 10s CFU/mL. Conclusion A two-tube MCMRT-PCR assay using six primer sets was developed for detection of multiple pathogens. Our findings demonstrates that the proposed two-tube assay is reliable, useful and rapid for simultaneous detection of six foodborne pathogenic bacteria with an intended application in provincial Centers for Diseases Control and Prevention （CDC）.

关键词： Detection Real-time PCR Melting curve Bacteria

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20个青贮玉米品种在陇东南地区的生产性能和营养价值评价

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草地学报 2024年第8期32卷 2620-2629页

作者：马学军左愈臻刘瑞刚陈平谢建鹏王斐何振富甘肃省农业科学院畜草与绿色农业研究所甘肃兰州730070 清水县畜牧兽医事务服务中心甘肃天水741000

为筛选出适合陇东南地区(甘肃天水)种植的优质高产青贮玉米(Zea mays)品种,本研究以20个青贮玉米品种为材料,测定并分析了各品种农艺性状、生物产量、营养物质含量及产量,并采用隶属函数法进行了综合评价。结果表明:粗蛋白含量与绿叶数... 详细信息

为筛选出适合陇东南地区(甘肃天水)种植的优质高产青贮玉米(Zea mays)品种,本研究以20个青贮玉米品种为材料,测定并分析了各品种农艺性状、生物产量、营养物质含量及产量,并采用隶属函数法进行了综合评价。结果表明:粗蛋白含量与绿叶数、果穗数、有效果穗数、果穗长、鲜果穗重和黄叶数显著相关(P<0.05);酸性洗涤纤维和中性洗涤纤维含量与株高、穗位高、全株叶片数和黄叶数极显著相关(P<0.01);淀粉含量与穗位高、全株叶片数、单株鲜重和株高显著相关(P<0.05)。本研究中鲜草产量达到60 t·hm^(-2)以上的品种有5个,干物质产量达到15 t·hm^(-2)以上的品种有6个,‘先玉1620’的总可消化营养物产量最高。综合评价得出,在本试验条件下,青贮综合生产性能高的品种为‘先玉1620’‘文玉3号’和‘科大101’,可以大面积在陇东南地区作为全株青贮玉米品种推广种植。

关键词：生物产量营养成分含量营养物质产量隶属函数综合评价

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Visual Detection of Murray Valley Encephalitis Virus by Reverse Transcription Loop-Mediated Isothermal Amplification

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Biomedical and Environmental Sciences 2015年第3期28卷 227-230页

作者： GONG Rui WANG Han Hua QIN Hong GUO Xiao Ping ma xue jun Hubei International Travel Healthcare Center National Key Lab of Ports Infectious Disease Detection Hubei Entry-Exit Inspection and Quarantine Bureau of China Key Laboratory for Medical Virology National Institute for Viral Disease Control and PreventionChinese Center for Disease Control and Prevention

A sensitive reverse transcription loop-mediated isothermal amplification （RT-LAMP） assay was developed for rapid visual detection of Murray valley encephalitis virus （MVEV） infection. The reaction was performed in one step in a single tube at 63 ~C for 60 min with the addition of the hydroxynaphthol blue （HNB） dye prior to amplification. The detection limit of the RT-LAMP assay was 100 copies per reaction based on 10-fold dilutions of in vitro transcribed RNA derived from a synthetic MVEV DNA template. No cross-reaction was observed with other encephalitis-associated viruses. The assay was further evaluated using spiked cerebrospinal fluid sample with pseudotype virus containing the NS5 gene of MVEV.

关键词： Murray Transcription valley Amplification encephalitis prior amplification primer accession isothermal

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Preparation and Initial Application of a Monoclonal Antibody Specific for a Newly Discovered Conserved Linear Epitope of Rabies Virus Nucleoprotein

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Biomedical and Environmental Sciences 2012年第1期25卷 98-103页

作者： LV Xin jun ma xue jun WANG Li Hua LI Hao SHEN Xin Xin YU Peng Cheng TANG Qing LIANG Guo Dong State Key Laboratory for Infectious Disease Prevention and Control Department of Viral Encephalitis Institute for Viral Disease Control and Prevention Chinese Centers for Disease Control and Prevention Beijing 102206 China State Key Laboratory for Molecular Virology and Genetic Engineering Department of Core Facility Institute for Viral Disease Control and Prevention Chinese Centers for Disease Control and Prevention Beijing 102206 China

Objective To prepare monoclonal antibodies against a newly discovered and conserved linear epitope of Rabies virus nucleoprotein and to use them in a rabies diagnostic test. Methods Synthetic peptide containing the epitope was used as immunogen to prepare hybridoma cell lines by classical hybridoma technology. Anti-peptide monoclonal antibodies produced in ascites of inoculated Balb/c mice were labeled with fluorescein isothiocyanate （FITC） after purification and used in fluorescent antibody test （FAT）. Results Two positive hybridoma cell lines, RVNP-mabl-CL and RVNP-mab2-CL, were obtained. RVNP- mabl-CL produced a higher concentration of monoclonal antibody RVNP-mabl in Balb/c ascites. FITC-labeled RVNP-mabl showed correct results on certain Rabies virus-positive canine brain tissue samples and cells of a small subclone of baby hamster kidney 21 cell line （BSR）. Conclusion FITC-labeled RVNP-mabl has potential application for laboratory diagnosis of rabies

关键词： Rabies virus Nucleoprotein Epitope Peptide Monoclonal antibody

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信息能量一体融合关键技术与电厂实践案例

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电信工程技术与标准化 2024年第2期37卷 78-82页

作者：刘玮马骠李天任石一涵张宏坤马学军中国移动通信集团设计院有限公司北京100080

信息能量一体融合是将能量、信息这两大驱动人类文明进步的主线深度交织、相互促进,通过融合创新催生新需求、新模式、新业态。作为信息基础设施供应商,如何利用各种信息技术手段高效赋能行业,实现能效最大化是研究重点。本文阐述了信... 详细信息

信息能量一体融合是将能量、信息这两大驱动人类文明进步的主线深度交织、相互促进,通过融合创新催生新需求、新模式、新业态。作为信息基础设施供应商,如何利用各种信息技术手段高效赋能行业,实现能效最大化是研究重点。本文阐述了信息能量融合的基本内涵,理论分析了信息流与能量流融合作用机理、协同优化设计两大关键技术。以电力发电为例,抽象出能效度量模型,给出关键影响因子,分析信息流、能量流在燃料管理、汽轮机发电等生产环节相互作用和协同优化的实现方法,并给出最终能效提升的实际效果。

关键词：信息能量融合电力生产单位发电能耗

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Evolution of pore systems in low-maturity oil shales during thermal upgrading--Quantified by dynamic SEM and machine learning

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Petroleum Science 2024年第3期21卷 1739-1750页

作者： jun Liu xue Bai Derek Elsworth Key Laboratory of Deep Underground Science and Engineering(Ministry of Education) Institute of New Energy and Low-Carbon TechnologySichuan UniversityChengdu610065SichuanChina International Quantum Academy Shenzhen518048GuangdongChina Shenzhen Institute of Advanced Electronic materials Shenzhen Institute of Advanced TechnologyChinese Academy of SciencesShenzhen518055GuangdongChina Department of Energy and Mineral Engineering Pennsylvania State University ParkPA 16802USA

In-situ upgrading by heating is feasible for low-maturity shale oil,where the pore space dynamically *** characterize this response for a heated substrate concurrently imaged by *** systematically follow the evolution of pore quantity,size(length,width and cross-sectional area),orientation,shape(aspect ratio,roundness and solidity)and their anisotropy—interpreted by machine *** indicate that heating generates new pores in both organic matter and inorganic ***,the newly formed pores are smaller than the original pores and thus reduce average lengths and widths of the bedding-parallel pore ***,the average pore lengths and widths are increased in the bedding-perpendicular ***,heating increases the cross-sectional area of pores in low-maturity oil shales,where this growth tendency fluctuates at300℃.In addition,the orientation and shape of the newly-formed heating-induced pores follow the habit of the original pores and follow the initial probability distributions of pore orientation and ***,limited anisotropy is detected in pore direction and shape,indicating similar modes of evolution both bedding-parallel and *** propose a straightforward but robust model to describe evolution of pore system in low-maturity oil shales during heating.

关键词： Low-maturity oil shale Pore elongation Organic matter pyrolysis In-situthermal upgrading Scanning electron microscopy(SEM) machine learning

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Some Convergence Properties for Weighted Sums of martingale Difference Random Vectors

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Acta mathematica Sinica,English Series 2024年第4期40卷 1127-1142页

作者： Yi WU xue jun WANG School of Big Data and Artificial Intelligence Chizhou UniversityChizhou 247000P.R.China School of Big Data and Statistics Anhui UniversityHefei 230601P.R.China

Let{X_(ni),F_(ni);1≤i≤n,n≥1}be an array of R^(d)martingale difference random vectors and{A_(ni),1≤i≤n,n≥1}be an array of m×d matrices of real *** this paper,the marcinkiewicz-Zygmund type weak law of large numb... 详细信息

关键词： martingale difference random vectors weighted sums marcinkiewicz–Zygmund type weak law of large numbers complete convergence strong law of large numbers multivariate simple linear regression model

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Rapid and Accurate Sequencing of Enterovirus Genomes Using MinION Nanopore Sequencer

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Biomedical and Environmental Sciences 2017年第10期30卷 718-726页

作者： WANG Ji KE Yue Hua ZHANG Yong HUANG Ke Qiang WANG Lei SHEN Xin Xin DONG Xiao Ping XU Wen Bo ma xue jun National Institute for Viral Disease Control and Prevention Chinese Center for Disease Control and PreventionBeijing 102206China Institute of Disease Control and Prevention Beijing 100071China Beijing Applied Biological Technologies Co. Ltd.Beijing 102206China

Objective Knowledge of an enterovirus genome sequence is very important in epidemiological investigation to identify transmission patterns and ascertain the extent of an outbreak. The MinION sequencer is increasingly used to sequence various viral pathogens in many clinical situations because of its long reads, portability, real-time accessibility of sequenced data, and very low initial costs. However, information is lacking on MinION sequencing of enterovirus genomes. Methods In this proof-of-concept study using Enterovirus 71 （EV71） and Coxsackievirus A16 （CA16） strains as examples, we established an amplicon-based whole genome sequencing method using MinION. We explored the accuracy, minimum sequencing time, discrimination and high-throughput sequencing ability of MinION, and compared its performance with Sanger sequencing. Results Within the first minute （min） of sequencing, the accuracy of MinION was 98.5% for the single EV71 strain and 94.12%-97.33% for 10 genetically-related CA16 strains. In as little as 14 min, 99% identity was reached for the single EV71 strain, and in 17 min （on average）, 99% identity was achieved for 10 CA16 strains in a single run. Conclusion MinION is suitable for whole genome sequencing of enteroviruses with sufficient accuracy and fine discrimination and has the potential as a fast, reliable and convenient method for routine use.

关键词： Nanopore sequencing MinION Enterovirus Single molecule sequencing Viral genome sequencing

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