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Expression and Characterization of HGV E2 cDNA in Pichia pastoris

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Wuhan University Journal of Natural Sciences 2002年第1期7卷 122-126页

作者： Wang Zhuo-hua ma hui-wen Tong Li-heng Peng Xi-liang College of Pharmacy Wuhan University Wuhan Hubei China Wuhan Research and Development Centre for Biotechnology Wuhan Hubei China

A 559 base pair fragment of cDNA locating at the putative E2 region of GBV-C/HGV was inserted intoPichia pastoris expression vector pPIC9K in the reading frame of α-factor secreting signal peptide. The recombinant expression plasmid pPIC9K-E2 was introduced intoP. pastoris GS 115 with electroporation and recombined with the host genome by homological recombination. The His+Mut+ recombinant yeasts were selected and cultivated in the BMMY medium. After 3 days induction with 0.5% methanol, the target protein (E2) accumulated up to 30% of total proteins in the supernatant. The expressed E2 protein was proved possessing antigenicity and high specificity with Western blot and ELISA probed with sera from the immunized rabbits and the patients infected by GBV-C/HGV.

关键词： HGV E2 Pichia pastoris expressoin

Numerical Simulation for Induction Refining Process of Metallurgical-Grade Silicon in Vacuum Furnace

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Journal of Iron and Steel Research International 2012年第S1期19卷 77-81页

作者： L Guo-qiang ma wen-hui WANG Hua YU wen-zhou YANG Xi Faculty of Metallurgy and Energy Engineering Kunming University of Science and Technology National Laboratory for Vacuum Metallurgy Kunming University of Science and Technology MOE's Engineering Research Center of Metallurgical Energy Conservation and Emission Reduction Kunming University of Science and Technology

The temperature and velocity distribution of melting pool fields is very important effect to the silicon purification in vacuum induction furnace.A numerical model for the electromagnetic-thermal-hydrodynamic coupling fields have been developed by using the finite element method(FEM)and a 2D numerical simulation for electromagnetic、 temperature and velocity fields of metallurgical-grade silicon melting in vacuum induction furnace were performed with a software Multi-physics Comsol 3.5a in this *** results showed that the temperature field was dependent observably on input power of coils and induction heating times and the maximum temperature gradient in melting pool was 215K in holding *** the silicon molted gradually a clockwise vortex was come into being for electromagnetic stirring in the smelting *** variation of velocity field in melting silicon is mainly influenced with the change of the current intensity and power *** numerical predications of temperature distribution are in good agreement with experiments.

关键词： metallurgical-grade silicon vacuum induction refining multi-fields coupling numerical simulation

Effects of Cu contamination on the electrical properties of multicrystalline silicon purified by directional solidification route

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Effects of Cu contamination on the electrical properties of ...

基于数据挖掘的马文辉治疗类风湿关节炎用药规律研究

2014中国功能材料科技与产业高层论坛

作者： Li Jiao Chen Xiu-hua ma wen-hui Zhang Cong Wei Kui-xian Faculty of Physical Science and Technology Yunnan University The National Engineering Laboratory for Vacuum Metallurgy Faculty of Metallurgical and Energy EngineeringKunming University of Science and Technology

ISBN: (纸本)9783038353522

The multicrystalline silicon wafers purified by directional solidification route were used to introduce copper *** resistivity and minority carrier lifetime of multicrystalline silicon wafers were investigated by four-point probe resistivity tester andμ-PCD,*** temperature,atmosphere and cooling rate were *** was found that copper contaminants have a greater impact on the electrical properties of multicrystalline *** results showed that copper impurities tend to exist at defect sites at high temperature,and high annealing temperature,argon atmosphere and slow cooling conditions make more impact on the electrical properties of multicrystalline silicon than low annealing temperature,air atmosphere and fast cooling.

关键词： multicrystalline silicon Copper contamination minority carrier lifetime resistivity

来源： cnki会议评论

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中华中医药杂志 2018年第3期33卷 1067-1069页

作者：范天田舒晓芳姚博马文辉山西中医药大学研究生部太原030024 长治市中医研究所附属医院山西长治047500 山西中医药大学附属医院太原030024

目的:基于中医传承辅助平台软件(V2.5),发掘马文辉治疗类风湿关节炎(RA)的组方规律及用药特色。方法:收集2015年1月至2017年1月就诊于山西中医药大学附属医院风湿科的RA患者信息,进行频数分析、聚类分析及关联分析。结果:收集样本147例... 详细信息

目的:基于中医传承辅助平台软件(V2.5),发掘马文辉治疗类风湿关节炎(RA)的组方规律及用药特色。方法:收集2015年1月至2017年1月就诊于山西中医药大学附属医院风湿科的RA患者信息,进行频数分析、聚类分析及关联分析。结果:收集样本147例,得到方剂172首,涉及中药148味,挖掘出核心组合15个,新处方5个。结论:马文辉治疗RA,基于协调疗法的原则,以营卫倾移为核心机制,遵循寒热并用、攻补兼施、扶正祛邪,擅用藤类药以祛风通络,继用补肝肾、益气血、调营卫以扶正固本,清热解毒与活血化瘀贯穿始终。

关键词：马文辉数据挖掘中医传承辅助平台系统类风湿关节炎用药规律

Quantum Chemistry Study on Benzimidazoledithi Derivatives' Selective Pre-enrichment of Cadmium Cation

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Chinese Journal of Structural Chemistry 2018年第12期37卷 1860-1868,1843页

作者： LI Shao-Yuan GENG Chao YANG Zi-Heng YANG Xi CHEN Xiu-Min CHEN Xiu-Hua ma wen-hui State Key Laboratory of Complex Nonferrous Metal Resources Clean Utilization Kunming 650093China Faculty of Physical Science and Technology Yunnan UniversityKunming 650091China Yunnan Provincial Energy Research Institute Co. Ltd. Kunming 650093China

This work reports the mechanism of benzimidazoledithi(BDT) derivatives’ selective pre-enrichment of Cd2+under the stimulation of glutathione(GSH). The geometric and electronic properties of five BDT-M2+complexes(M = Cd, Cu, Hg, Pb, Co) have been investigated using density functional theory(DFT) at the B3 LYP/6-311 G(d,p) level with the GAUSSIAN 09 package program. The results show that BDT ligand exhibits alternative behaviors to different metal ions with the binding affinity in the order of Cu2+> Cd2+> Pb2+> Hg2+> Co2+. After adding the BDT-M2+complex into the GSH solution, the new S–S bonds can be formed, resulting with benzimidazole-metal ions(MBI-M2+) falling off into the GSH solution. Furthermore, the weak interaction between the new glutathione derivative(GSHD) and MBI-M2+were found. However, the strong chelation was detected between GSHD and MBI-M2+(M = Cu, Pb, Hg, Co) to hinder the adsorbed Cu2+, Pb2+and Hg2+, Co2+completely falling into the GSH solution, which suggests porous silicon composite modified by BDT has a certain selective pre-enrichment of Cd2+ion.

关键词： benzimidazoledithi(BDT) derivatives selective pre-enrichment glutathione cadmium ion density functional theory

Phosphorus Removal from Metallurgical-Grade Silicon by Vacuum Arc Refining

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Journal of Iron and Steel Research International 2012年第S2期19卷 925-928页

作者： WEI Kui-xian ma wen-hui YANG Bin LIU Da-chun DAI Yong-nian The National Engineering Laboratory for Vacuum Metallurgy Faculty of Metallurgical and Energy EngineeringKunming University of Science and Technology Key Laboratory for Nonferrous Vacuum Metallurgy of Yunnan Province Engineering Research Center for Silicon Metallurgy and Silicon materials of Yunnan Provincial Universities

Phosphorus is very difficult to remove during the purifying of metallurgical-grade silicon by metallurgical *** this paper,phosphorus evaporation removal by vacuum arc furnace with electromagnetic stirring was investigated in vacuum weak oxidation *** effects of refining time,electromagnetic stirring treatment and initial phosphorus content on the removal behavior of phosphorus in metallurgical-grade silicon were studied in detial. The result shows that the reduction of phosphorus using vacuum arc refining method was very obvious,especially in the first 10 minutes,and the removal rate of phosphorus increased with the refining *** stirring is also benefit to the removal of phosphorus evaporation,which can be attributed to the improved mobility of phosphorus element from the internal to the surface(i.e.,improved kinetics condition)of *** was also observed that high purity sample could be obtained in the initial materials with low phosphorus content.

关键词： Vacuum arc refining Silicon melts Phosphorus Electromagnetic stirring

Boron Removal From Metallurgical Grade Silicon by Molten Slag Refining in Induction Furnace

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Journal of Iron and Steel Research International 2012年第S2期19卷 779-782页

作者： WU Ji-jun ma wen-hui YANG Bin LIU Da-chun DAI Yong-nian The National Engineering Laboratory for Vacuum Metallurgy Faculty of Metallurgical and Energy Engineering Kunming University of Science and Technology Key Laboratory for Nonferrous Vacuum Metallurgy of Yunnan Province Engineering Research Center for Silicon Metallurgy and Silicon materials of Yunnan Provincial Universities

The impurities Al,Ca,Ti,B,P etc in metallurgical grade silicon(MG-Si)can be effectively removed by refining using molten slag based CaO-SiO_2,and it is especially effective for boron *** experiments of boron removal were studied using CaO-SiO_2 binary slag in induction *** results showed that the distribution coefficient of boron(L_B)between slag and silicon increased with more proportion of CaO/SiO_2(mass%).It was advantaged to boron removal for higher basicity of slag,so the boron in MG-Si was reduced from 18ppmw to 1.4ppmw with the addition of Li_2O and K_2O to CaO-SiO_2 *** proportion of SiO_2 in slag affected the oxidizing capacity of slag,which reduced the efficiency of boron removal.

关键词： Metallurgical grade silicon boron slag refining basicity induction furnace

Boron Removal From Metallurgical-Grade Silicon Using CaO-SiO_2 Slag

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Journal of Iron and Steel Research International 2012年第S2期19卷 762-765页

作者： DING Zhao WEI Kui-xian ma wen-hui WU Ji-jun XIE Ke-qiang ZHOU Yang The National Engineering Laboratory for Vacuum Metallurgy Faculty of Metallurgical and Energy Engineering Kunming University of Science and Technology Engineering Research Center for Silicon Metallurgy and Silicon materials of Yunnan Provincial Universities Kunming University of Science and Technology

The removal of boron impurity from metallurgical-grade silicon for solar cell application in slag system of CaO-SiO_2 is *** experiments are conducted in an electromagnetic induction furnace which is used to heat. The distribution coefficient of boron(L_B)between slag and silicon phase is particularly examined in terms of the optical basicity of *** the increase of optical basicity,L_B increases to a local maximum value of 1.58 when the optical basicity is 0.71 after getting to the minimum value of 0.72 when the optical basicity is *** that above optical basicity of 0.71,L_B decrease sharply which indicates that increasing the basicity of slag is not always effective in boron removal from silicon.

关键词： metallurgical-grade silicon slag refining distribution coefficient boron CaO-SiO_2

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马文辉教授治疗消化性溃疡的临证经验

世界最新医学信息文摘 2019年第12期19卷 165-166页

作者：姜建忠马文辉山西中医药大学研究生部山西中医学院附属医院风湿科

溃疡消化性是临床常见的消化性疾病之一,发病率、复发率较高。临床上根据消化性溃疡的发病机制、病理特点、临床表现的不同,其治疗方案也不同,并且中医和现代医学对消化性溃疡的认识及治疗也各有不同。马文辉教授在临床实践过程中对消... 详细信息

溃疡消化性是临床常见的消化性疾病之一,发病率、复发率较高。临床上根据消化性溃疡的发病机制、病理特点、临床表现的不同,其治疗方案也不同,并且中医和现代医学对消化性溃疡的认识及治疗也各有不同。马文辉教授在临床实践过程中对消化性溃疡的病因病机以及治疗上有独特的认识和体会。马教授针对情志因素导致的肝胃不和型消化性溃疡,运用疏肝理气,和胃止痛疗法,在临床中取得良好疗效。

关键词：马文辉消化性溃疡肝胃不和型临床经验