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Expression of aquaporin-1 in SMMC-7221 liver carcinoma cells promotes cell migration

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Chinese Science Bulletin 2006年第20期51卷 2466-2471页

作者： LI Yongming FENG Xuechao YANG Hong ma tonghui Membrane Channel Research Laboratory Northeast Normal UniversityChangchun 130024 China

Migration of tumor cells is a crucial step in tumor invasion and metastasis. Here we provide evidence that aquaporin expression is involved in tumor cell migration. RT-PCR, immunofluorescence and Western blot analysis demonstrated the AQP1 protein expression on the plasma membrane of SMMC-7221 human hepatoma cells. SMMC-7221 cell clones with high (SMMC-7221hPf) and low (SMMC-7221lPf) water permeability were identified by functional assays with corresponding high and low AQP1 expression. Cell migration rate was remarka- bly higher in SMMC-7221hPf cells than SMMC-7221l Pf cells, assessed by Boyden chamber and wound healing assays, whereas cell growth and adhesion were not different. Adenovirus-mediated AQP1 ex- pression in SMMC-7221lPf cells increased their water permeability and migration rate. These results pro- vide the first evidence that aquaporin-mediated membrane water permeability enhances tumor cell migration and may be associated with tumor invasion and metastasis.

关键词：细胞迁移瘤转移细胞容积肝肿瘤 SMMC-7221

Progress in studies of angio-statin and its anti-tumor effects

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Chinese Science Bulletin 2001年第6期46卷 454-460页

作者： Hong Yang Yin Wu Yuxin Li tonghui ma Baiqu Huang NE Normal Univ Inst Genet & Cytol Changchun 130024 Peoples R China

Angiostatin is a 38 ku circulating angiogenesis inhibitor purified from the serum and urine of mice bearing a murine Lewis lung carcinoma. It is regarded as an internal fragment of plasminogen containing the first 4 Kringle structures. In vitro, angiostatin specifically inhibits endothe-lial cell proliferation. In vivo, it inhibits angiogenesis and suppresses the growth of such primary tumors as Lewis lung carcinoma and hemangioendotheliolma in mice.

关键词： angiostatin angiogenesis endothelial cell anti-tumor.

Identification of Cinobufagin and Resibufogenin as Inhibitors of Enterovirus 71 Infection

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Chemical Research in Chinese Universities 2014年第6期30卷 953-958页

作者： CHEN Jiawen XU Lin SUN Shiyang ZHANG Huafei ma tonghui SU Weiheng JIANG Chunlai National Engineering Laboratory for AIDS Vaccine Key Laboratory for Molecular Enzymology & Engineering Ministry of Education School of Life Seiences Jilin University Changchun 130012 P. R. China" College of Basic Medical Sciences Dalian Medical University Dalian 116044 t~ R. China

In this paper, cinobufagin and resibufogenin were found to inhibit enterovirus 71（EV71） infection in vitro in cell viability and plaque reduction assays. The 50% inhibitory concentrations（lCs0） of einobufagin and resibufoge- nin were （10.94±2.36） and （218±31） nmol/L, respectively, the 50% cytotoxic concentrations（CCs0） of them were （1277±223） and （1385±254） nmol/L, respectively, and the anti-EV71 selectivity index（SI50） of cinobufagin was 116.7, which are promisingly developed into drug. Using a VP1 detection assay and a constructed reporter luciferase, we found that cinobufagin and resibufogenin disrupted the synthesis of EV71 protein. However, neither of them inhibited EV71 RNA replication. Our study suggests that cinobufagin and resibufogenin are the promising candidates that should he fllrther investigated for the treatment of EV71 caused disease.

关键词： Enterovirus 71 Coxsackievirus A16 Cinobufagin Resibufogenin Chansu

EFFECTS OF TRANSPORT STRESS ON THE INTESTINES INVOLVING NEURONAL NITRIC OXIDE SYNTHASE

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Frontiers of Agricultural Science and Engineering 2023年第2期10卷 285-295页

作者： Jing LAN tonghui ma Peng YIN Kedao TENG Yunfei ma College of Veterinary Medicine China Agricultural UniversityBeijing 100193China School of Engineering Medicine Beihang UniversityBeijing 100191China

Transport stress is commonly suffered by animals with gastrointestinal dysfunction a common ***,the mechanisms of transport stress-induced intestine impairment are largely *** aim of this study was to investigate the effects of transport stress on the expression of neuronal nitric oxide synthase(nNOS)and the distribution of nNOS-positive neurons of the intestines in rats and to explore the neuroendocrine mechanism of transport *** this study,Sprague Dawley rats(n=6)were subjected on a constant temperature shaker for 1(S1d)or 3 d(S3d).Rats exhibited increased serum glucose and diminished total number of leukocytes,in which lymphocytes level was also decreased in the S1d group(Pmal intestinal morphology was disrupted in the S1d rats,and the thickness of muscle layers was decreased in duodenum,jejunum and colon of S3d *** addition,it was found that nNOS expression,as well as the number of nNOSpositive neurons in the myenteric plexus were downregulated in duodenum,jejunum and colon of S3d rats compared with that of unstressed rats(Pmage and uncovers potential action mechanisms that nNOS-positive neurons and nNOS expression might be involved in modulating this process.

关键词： intestine nNOS nNOS-positive neurons transport stress

Detection of anti-aquaporin-4 autoantibodies in the sera of Chinese neuromyelitis optica patients

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Neural Regeneration Research 2013年第8期8卷 708-713页

作者： Miao Li Weiheng Su Jie Wang Francesco Pisani Antonio Frigeri tonghui ma Membrane Channel Research Laboratory Northeast Normal University Department of Neurosurgery China-Japan Union HospitalJilin University National Engineering Laboratory for AIDS Vaccine College of Life ScienceJilin University Department of General and Environmental Physiology and Centre of Excellence in Comparative Genomics (CEGBA) University of Bari

In this study, we recruited 10 neuromyelitis optica patients, two multiple sclerosis patients and two myelitis patients. Chinese hamster lung fibroblast （V79） cells transfected with a human aquaporin-4-mCherry fusion protein gene were used to detect anti-aquaporin-4 antibody in neuromyelitis optica patient sera by immunofluorescence. Anti-aquaporin-4 autoantibody was stably detected by immunofluorescence in neuromyelitis optica patient sera exclusively. The sensitivity of the assay for neuromyelitis optica was 90% and the specificity for neuromyelitis optica was 100%. The anti-aquaporin-4 antibody titers in sera were tested with serial dilutions until the signal disappeared. A positive correlation was detected between Expanded Disability Status Scale scores and serum anti-aquaporin-4 antibody titers. The anti-aquaporin-4 antibody assay is highly sensitive and specific in the sera of Chinese neuromyelitis optica patients. Detection of aquaporin-4 autoantibody is important for the diagnosis and treatment of neuromyelitis optica.

关键词： neural regeneration neurodegenerative diseases neuromyelitis optica multiple sclerosis aquaporin-4 autoimmune disease autoantibody immunofluorescence Expanded Disability StatusScale grants-supported paper photographs-containing paper neuroregeneration

Characteristics of TP53 germline variants and their correlations with Li-Fraumeni syndrome or Li-Fraumeni-like syndrome in Chinese tumor patients

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Journal of Genetics and Genomics 2022年第7期49卷 645-653页

作者： Panwen Tian Xiaoyan Zhang Sheng Yang Yu Fang Hongling Yuan Wei Li Honglin Zhu Fangping Zhao Jinlei Ding Yunshu Zhu Sizhen Wang Guochen Sun Hongbin Ni tonghui ma Ting Lei Department of Respiratory and Critical Care Medicine Lung Cancer Treatment CenterWest China HospitalSichuan UniversityChengduSichuan 610041China Department of Translational Medicine Genetron Health(Beijing)TechnologyCo.Ltd.Beijing 102206China National Cancer Center/National Clinical Research Center for Cancer/Cancer Hospital Chinese Academy of Medical Sciences and Peking Union Medical CollegeBeijing 100005China Department of Thoracic Surgery The Second Hospital of Dalian Medical UniversityDalianLiaoning 116023China Genetron Health(Beijing)Technology Co.Ltd.Beijing 102206China Department of Neurosurgery The First Medical Center of PLA General HospitalBeijing 100853China Department of Neurosurgery Nanjing Drum Tower Hospital(Nanjing Gulou Yi Yuan)The Affiliated Hospital of Nanjing University Medical SchoolNanjing 210008China

Li-Fraumeni syndrome(LFS),a rare autosomal-dominant inheritance condition,is associated with a family cancer history as well as pathogenic/likely-pathogenic TP53 germline variants(P/LP TP53 GV).The current clinical methods for detecting LFS are ***,we retrospectively investigate P/LP TP53 GV among Chinese cancer patients by next-generation sequencing and evaluate its relationship with a family cancer history.A total of 270 out of 19,226 cancer patients have TP53 GV,including 53 patients with P/LP TP53 *** with P/LP TP53 GV are mainly found in male with glioma,lung cancer or *** median age of diagnosis for P/LP TP53 GV patients is significantly lower than that of non-P/LP TP53 GV patients(31-years vs.53-years;P<0.01).One LFS patient and 3 Li-Fraumeni-like syndrome(LFL)patients are among the 26 followed-up P/LP TP53 GV *** 25 types of P/LP TP53 GV,the highest variant frequencies occurred at codon 175 and 248.p.M237 I,p.R158 H,p.C238 Y and p.C275 R,are firstly identified among the Chinese LFS/LFL *** study reports the(P/LP)TP53 GV characteristics of Chinese pan-cancer *** findings suggest analyzing the P/LP TP53 GV in cancer patients is an effective strategy for identifying cancer predisposition syndrome.

关键词： Li-Fraumeni syndrome TP53 germline variants Pathogenic Likely-pathogenic Family cancer history

The hepatotoxic effect of bile acids can be largely attributed to direct i nflammasome activation in hepatocytes and Kupffer cells

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The hepatotoxic effect of bile acids can be largely attribut...

中国自然保护地空间重叠分析与保护地体系优化整合对策

2015年浙江省外科学学术年会暨国家级肝胆胰疾病诊治进展学习班

作者： Xiqiang Liu tonghui ma Timothy R.Billiar Dongsheng Huang Ran Tao Center for Organ Transplantation and Department of Surgery Ruijin HospitalShanghai Jiaotong University School of Medicine Department of Hepatobiliary-Pancreatic Surgery Zhejiang Provincial People' s Hospital(ZJPPH)

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关键词： bile acid hepatocytes inflammasome Kupffer cell

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生物多样性 2019年第7期27卷 758-771页

作者：马童慧吕偲雷光春北京林业大学自然保护区学院

自然保护地体系的构建是国际社会高度重视的生物多样性保护策略。近年来,中国已关注到自然保护地空间重叠交叉的问题,并出台了《关于建立以国家公园为主体的自然保护地体系的指导意见》。为落实这一战略,需要对现有保护地的彼此关系与... 详细信息

自然保护地体系的构建是国际社会高度重视的生物多样性保护策略。近年来,中国已关注到自然保护地空间重叠交叉的问题,并出台了《关于建立以国家公园为主体的自然保护地体系的指导意见》。为落实这一战略,需要对现有保护地的彼此关系与空间分布进行系统研究。为此,本研究收集了8,572个不同类型和级别自然保护地的坐标、生态系统类型、行政区域及边界等信息,筛选出1,532个具有空间重叠、管理部门交叉的自然保护地,计算地理集中指数并采用Arc GIS软件进行了核密度分析,得出空间重叠保护地分布的生态地理区、生态系统类型、交叉管理部门、所在省份等空间分布特点。研究结果显示:(1)鲁中山区、太行山、大别山、天目山–怀玉山、皖江等生态功能区的自然保护地重叠最为严重(核密度Mean> 6, max> 8),其中太行山区、大别山区、天目山–怀玉山区为重叠保护地密度高的生物多样性优先保护区,目前10个国家公园试点区域中仅大熊猫国家公园体制试点区、湖南南山国家公园体制试点区、钱江源国家公园体制试点区三处位于保护地重叠高密度区;(2)原主管部门中,原国家林业局与住房和城乡建设部的交叉管理保护地数量最多,为294个;(3)黑龙江、安徽、山东、河南、湖北、湖南等省范围内的自然保护地空间重叠状况明显高于其他省区,而晋冀豫与皖鄂赣这两处三省交界处重叠程度更高,其他多处三省交界区域也存在保护地的中度重叠。故上述生态地理区、原主管部门与行政区应作为中国自然保护地体系优先普查区域与优化整合重点对象。基于重叠保护地核密度热点区、生物多样性保护优先区与生态系统文化服务的分析框架,本研究按照国家公园、自然保护区、自然公园三类,对不同生态功能区自然保护地优化整合优先性与类型提出了初步建议,以期为当前中国自然保护地体系改革的紧迫需求提供参考。

关键词：自然保护地空间重叠交叉管理整合国家公园

AdMSC-derived exosomes alleviate acute lung injury through transferring mitochondrial respiratory component to improve bioenergetics and immune homeostasis of alveolar macrophages

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AdMSC-derived exosomes alleviate acute lung injury through t...