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Serum ECM1 is a promising biomarker for staging and monitoring fibrosis in patients with chronic hepatitis B

Science China Life Sciences 2024年

作者： Lian Liu Danyan Zhang Rong Fan Shipeng Cheng Jichao Yang liyan ma Zhiyang Ling Yaguang Zhang Jinlin Hou Xiaomei Wang Bing Sun Junqi Niu Shanghai Institute of Biochemistry and Cell Biology Centre for Excellence in Molecular Cell Science Chinese Academy of Sciences University of Chinese Academy of Sciences School of Life Science and Technology ShanghaiTech University Department of Infectious Disease Nanfang Hospital Southern Medical University Med-X Institute Centre for Immunological and Metabolic Diseases The First Affiliated Hospital of Xi'an Jiaotong University Xi'an Jiaotong University Hepatology Department Centre of Infectious Diseases and Pathogen Biology First Hospital of Jilin University

It is critical to assess the extent and progression of liver fibrosis for patients to receive suitable treatments, but its diagnostic methods remain unmet. Extracellular matrix protein 1（ECM1） has previously been reported to be a key factor in the induction and progression of liver fibrosis. However, little is known about the use of ECM1 as a biomarker to evaluate fibrosis. In a CCl4-induced mouse model of liver fibrosis, the present study demonstrated that ECM1 decreased with gradually increasing fibrosis. Using biopsy as a reference, the serum ECM1 levels decreased with increasing fibrosis stage in 247 patients with liver fibrosis, but there were no significant changes between fibrosis stage 2 and stage 0–1. To improve the performance of ECM1, age, platelet count, and ECM1 concentration were combined to calculate an EPA（ECM1-platelet-age） score（ranging from 0 to 10）. The areas under the receiver operating characteristic curve of the EPA scores for the detection of F≥2, F≥3, and F4 were 0.6801, 0.7377, and 0.8083, respectively, which showed a comparable or significantly greater diagnostic performance for assessing fibrosis than that of the AST/ALT ratio, APRI score, or FIB-4 score. In HBV patients following antiviral treatment, the dynamics of the EPA score depended on the status of liver fibrosis development. The accuracy of the EPA score in predicting fibrosis regression and progression was 66.00% and 71.43%, respectively, while that of the LSM, another useful method for monitoring hepatic fibrosis changes during treatment, was only 52.00% and 7.14%, respectively. Compared with healthy controls, there were lower levels of serum ECM1 in HBV patients and individuals with HCV infection, maFLD, ALD, PBC, and DILI. These findings suggested that individuals with reduced ECM1 levels may have a risk of developing liver injury, and further examinations or medical care are *** conclusion, the ECM1-containing EPA score is a valuable noninvasive test for staging fibros

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以多发动脉血管病变为首发表现的儿童白塞病1例

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中华儿科杂志 2024年第5期62卷 476-477页

作者：王莲马爱玲张惠马立燕赖建铭宁夏医科大学总医院儿科银川750004 宁夏医科大学总医院病理科银川750004 宁夏医科大学总医院超声科银川750004 首都儿科研究所附属儿童医院风湿免疫科北京100020

患儿女,11岁3月龄,因反复下肢疼痛3个月入院,病程期间出现口腔溃疡、眼部溃疡及便血现象。血管彩色多普勒超声示左侧锁骨下动脉近心段管壁增厚,下肢动脉彩色多普勒超声示双侧股动脉局部管壁增厚,双侧胫后动脉中远段重度狭窄伴节段性闭塞... 详细信息

患儿女,11岁3月龄,因反复下肢疼痛3个月入院,病程期间出现口腔溃疡、眼部溃疡及便血现象。血管彩色多普勒超声示左侧锁骨下动脉近心段管壁增厚,下肢动脉彩色多普勒超声示双侧股动脉局部管壁增厚,双侧胫后动脉中远段重度狭窄伴节段性闭塞,双侧胫前动脉狭窄可能。电子结肠镜检查示回肠末端、盲肠及乙状结肠、直肠黏膜血水肿,散在多发糜烂和浅溃疡形成。给予醋酸泼尼松片及环磷酰胺,3周后双下肢疼痛缓解,可正常行走,足部溃疡逐渐好转,随访1年后全身血管超声均未见异常。

关键词：醋酸泼尼松片双下肢疼痛管壁增厚双侧股动脉胫后动脉足部溃疡白塞病血管超声

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Multi-vision Attention Networks for on-Line Red Jujube Grading

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Chinese Journal of Electronics 2019年第6期28卷 1108-1117页

作者： SUN Xiaoye ma liyan LI Gongyan Institute of Microelectronics of Chinese Academy of Sciences University of Chinese Academy of Sciences School of Computer Engineering and Science Shanghai University

To solve the red jujube classification problem, this paper designs a convolutional neural network model with low computational cost and high classification accuracy. The architecture of the model is inspired by the multi-visual mechanism of the organism and Dense *** further improve our model, we add the attention mechanism of SE-Net. We also construct a dataset which contains 23,735 red jujube images captured by a jujube grading system. According to the appearance of the jujube and the characteristics of the grading system, the dataset is divided into four classes: invalid, rotten, wizened and normal. The numerical experiments show that the classification accuracy of our model reaches to 91.89%,which is comparable to Dense Net-121, Inception V3,Inception V4, and Inception-Res Net v2. Our model has real-time performance.

关键词： Convolutional neural network(CNN) Deep learning Red jujube classification Real-time

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vWF:Ag国产试剂盒的性能验证研究

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标记免疫分析与临床 2024年第5期31卷 917-921,977页

作者：杨伟民马立艳首都医科大学附属北京友谊医院临床检验中心北京100050

目的评价国产vWF:Ag(胶乳免疫比浊法)试剂盒在进口血凝检测系统上的检测性能。方法共收集2023年4月至7月北京友谊医院西城院区157例住院患者和40例表观健康人员的枸橼酸钠抗凝血浆样本。vWF:Ag试剂检测质控在靶值宣称的范围内,再进行试... 详细信息

目的评价国产vWF:Ag(胶乳免疫比浊法)试剂盒在进口血凝检测系统上的检测性能。方法共收集2023年4月至7月北京友谊医院西城院区157例住院患者和40例表观健康人员的枸橼酸钠抗凝血浆样本。vWF:Ag试剂检测质控在靶值宣称的范围内,再进行试剂的性能验证。选取vWF:Ag国产试剂质控品进行精密度评价;选取北京九强公司的工作校准品进行正确度验证;选取高、低值临床样本进行线性范围评价;选取表观健康人群样本进行参考区间验证;选取50例高、中、低值均匀分布临床样本,将国产试剂和进口试剂的结果进行比对,并计算两者相关性和相对偏差;对国产试剂盒对干扰物质(直接胆红素、间接胆红素、血红蛋白、脂肪乳)的抗干扰性能进行验证。结果vWF:Ag国产试剂高、中、低3个质控浓度的批内精密度CV、批间精密度CV和总精密度CV均<10%。正确度两个水平的工作校准品与靶值的偏倚分别为-2.0%和3.3%,均<±10%。线性范围相关系数大于0.995,偏差<±15%,验证的线性范围为10%~253%。O型血人群参考范围为47.8%~138.5%、非O型血人群参考范围为76.5%~163.0%,验证通过。干扰物质胆红素≤40mg/dL,血红蛋白≤150mg/dL,脂肪乳≤600mg/dL,回收率在90%~110%之间,相对偏差<±10%,满足试剂要求。结论在沃芬全自动血凝分析仪ACL TOP500检测系统上,vWF:Ag(胶乳免疫比浊法)国产试剂盒性能良好,符合实验室检测质量要求,满足临床检测需求。

关键词： vWF:Ag 国产体外诊断试剂性能验证

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Migration of bisphenol A and its related compounds in canned seafood and dietary exposure estimation

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Food Quality and Safety 2022年第2期6卷 275-286页

作者： Na Lin Derong ma Zhidong Liu Xichang Wang liyan ma East China Sea Fisheries Research Institute Chinese Academy of Fishery SciencesShanghaiChina Shanghai Ocean University ShanghaiChina

The present study sought to investigate the migration of target bisphenols,such as bisphenol-A(BPA),bisphenol-F(BPA),bisphenol-A diglycidyI ether(BADGE),bisphenol-A(2,3-dihydroxypropyl)glycidyl ether(BADGE.H_(2)O),bisphenol-A bis(2,3-dihydroxypropyl)ether(BADGE.2H_(2)O),and bisphenol F diglycidyl ether(BFDGE)in 102 samples of several canned seafood,namely canned Antarctic krill,scallop,oysters,mussel,clam,and mantis shrimp stored for months at different temperatures through a high-performance liquid chromatographic-fluorescence detector com-bined with a microwave-assisted extraction *** for BFDGE,the other five bisphenols were observed in most of the analyzed *** canned shrimp showed the highest migration of BPA(0.089 mg/kg),exceeding the specific migration limit(SML)of BPA(0.05 mg/kg)specified by the European Union,while the migration levels of BADGE and its derivatives were within their *** migration behavior of bisphenols in the canned seafood was majorly affected by the analytes,storage conditions,and food ***,BADGE.H_(2)O,and BADGE 2H_(2)O were characterized by a rapid migration during the first half of the shelf life,which increased with the increase of temperature,followed by a stabilization or decline of their concentrations for prolonged ***,the migration of target bisphenols was significantly influenced by the storage temperature in some seafood ***,higher migration level of BPA was found in samples with higher fat *** average dietary exposure of Chinese adults to BPA,BPF,BADGE.2H_(2)O,BADGE.H_(2)O,and BADGE of canned seafood was estimated at 11.69,1.21,6.47,8.74,and 4.71 ng/(kg d),*** target hazard quotient values of all the analyzed bisphenols were below 1 for the Chinese adults,suggesting an insignificant exposure to these bisphenols through canned seafood consumption.

关键词： Bisphenol-A(BPA) BPA-related compounds canned seafood migration behavior dietary exposure

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Effect of Co substitution on the glass forming ability and magnetocaloric effect of Fe88Zr8B4 amorphous alloys

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Science China(Physics,Mechanics & Astronomy) 2017年第7期60卷 81-84页

作者： LiangHua Gan liyan ma BenZhen Tang Ding Ding Lei Xia Laboratory for Microstructure Institute of Materials Shanghai University Shanghai 200072 China

As greater attention is paid to energy consumption and global warming, magnetic refrigeration （MR） technologies based on the magneto-caloric effect （MCE） have been developed. Systems based on MR are expected to be more compact, energy efficient, and environmentally safe compared with traditional vapor-cycle refrigeration technologies .

关键词： Effect of Co substitution on the glass forming ability and magnetocaloric effect of Fe Co Figure Zr8B4 amorphous alloys

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Application of Pb isotopic tracing technique to constraining the source of Pb in the West Lake Longjing tea

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Chinese Journal Of Geochemistry 2011年第4期30卷 554-562页

作者： LU Yuanfa YANG Hongmei ma liyan CHEN Xiqing WANG Qunying Department of Geochemistry Yangtze University Jingzhou 434023 China Key Laboratory of Exploration Technologies for Oil and Gas Resources (Yangtze University) Ministry of Education Jingzhou 434023 China Yichang Institute of Geology and Mmeral Resources Yichang 443003 China

This paper dealt with the Pb contents and Pb isotopic composition of the West Lake Longjing tea. The results showed that in the tea leaves, from young leaf →old leaf →tea limb, the Pb contents tend to increase gradu-ally from 1.63 →4.84 →6.07×10-6, wich revealed that the Pb was accumulated gradually in the tea. After cleaned, the Pb contents of tea leaves were significantly reduced. This indicated that the deposits on the surface of tea leaves made a great contributuion to Pb contents. The survey results for soils in the relevant tea gardens showed that soil from the Longjing tea garden has higher Pb contents, with an average level of 49.6×10-6, two times those of common soils (24×10-6) in China. Results of the systematic analysis for tea, tea garden soil and the samples with the relevant background of the Pb isotopic composition displayed that the Pb isotopic ratio of tea is 206Pb/207Pb=1.164±0.005 (2σ). The ratio of 206Pb/207Pb for the soil gradually decreased from residue phase, soil dilute acid extract phase, and then to urban topsoil, i.e., 1.175 →1.171 →1.170. The 208Pb/(206Pb+207Pb) ratios also show a similar variation trend. 206Pb/207Pb ratios in the samples with the relevant background were: vehicle exhaust, 1.124; coal-combustion, 1.156; atmosphere, 1.168; and water, 1.166. Comparative studies have shown that Pb pollution is popular in the environ-mental media (soil, atmosphere, water) in Hangzhou. With the aggravation of Pb pollution, the Pb isotopic composi-tion gradually changed from the natural background (soil residues) to the direction of automobile exhaust. This phe-nomenon could illustrate that the pollution source was the vehicle exhaust, while the coal-combustion contributed little to environmental pollution in Hangzhou. The Pb of the Longjing tea came mostly from soluble phase Pb in the polluted soil. Moreover, secondary pollution was caused by vehicle exhaust.

关键词：铅同位素组成西湖龙井茶同位素示踪技术茶园土壤汽车尾气应用铅同位素比值土壤残留

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乳腺数字X射线联合MRI对Bi-RADS 4级可疑恶性微钙化患者的诊断价值

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中国医药导报 2024年第18期21卷 156-158,163页

作者：刘兆丰金静强马立岩江苏省昆山市第二人民医院放射科江苏昆山215300

目的探讨乳腺数字X射线联合磁共振成像(MRI)评估Bi-RADS 4级可疑恶性微钙化的诊断价值。方法选取2021年6月至2023年7月江苏省昆山市第二人民医院行手术治疗的79例乳腺Bi-RADS 4级可疑恶性微钙化患者为研究对象,所有患者均进行乳腺数字X... 详细信息

目的探讨乳腺数字X射线联合磁共振成像(MRI)评估Bi-RADS 4级可疑恶性微钙化的诊断价值。方法选取2021年6月至2023年7月江苏省昆山市第二人民医院行手术治疗的79例乳腺Bi-RADS 4级可疑恶性微钙化患者为研究对象,所有患者均进行乳腺数字X射线、MRI检查。以病理结果为“金标准”,分析良性、恶性患者乳腺数字X射线图像特征、MRI特征,分析不同检查方法的诊断效能。结果以病理检查结果为“金标准”,恶性患者34例,良性患者45例,其中乳腺纤维腺瘤21例、乳腺囊性增生12例、乳腺炎12例。良、恶性患者乳腺微钙化、无定型、粗糙不均质分布比较,差异有统计学意义(P<0.05)。良、恶性患者时间-信号强度曲线各型占比比较,差异有统计学意义(P<0.05)。乳腺数字X射线联合MRI检查的特异度、准确度高于单一检测,差异有统计学意义(P<0.05)。乳腺数字X射线、MRI、乳腺数字X射线联合MRI检查与“金标准”一致性一般,Kappa值分别为0.625、0.630、0.726。结论乳腺数字X射线联合MRI检查诊断Bi-RADS 4级可疑恶性微钙化的价值较高,有助于临床医生准确地判断可疑微钙化患者的恶性可能性。

关键词：乳腺数字X射线磁共振成像 Bi-RADS 4级恶性微钙化

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北京某医院感染性腹泻患者致泻性大肠埃希菌毒力和耐药特征分析

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临床检验杂志 2024年第1期42卷 43-47页

作者：马思宇马立艳罗虎周妍妍王雅轩苏建荣首都医科大学附属北京友谊医院临床检验中心北京100050 北京医院国家老年医学中心北京100730 国家卫生健康委临床检验中心北京100730 中国医学科学院老年医学研究院北京100730

目的明确本院感染性腹泻患者致泻性大肠埃希菌毒力和耐药特征。方法采用VITEK MS微生物质谱检测系统初步鉴定,多重实时荧光PCR检测毒力基因,对本院感染性腹泻患者临床分离的致泻性大肠埃希菌(Diarrheagenic Escherichia coli,DEC)进行5... 详细信息

目的明确本院感染性腹泻患者致泻性大肠埃希菌毒力和耐药特征。方法采用VITEK MS微生物质谱检测系统初步鉴定,多重实时荧光PCR检测毒力基因,对本院感染性腹泻患者临床分离的致泻性大肠埃希菌(Diarrheagenic Escherichia coli,DEC)进行5种型别鉴定。微量肉汤稀释法和E-test法药敏试验检测DEC菌株的耐药表型特征。二代测序及生物信息学分析其耐药分子特征。采用Fisher确切概率法进行统计学分析。结果本院DEC检出率为11.9%,其中EAEC占比37.5%,非典型EPEC占比34.38%,ETEC占比25.0%,EIEC占比3.12%,未检出EHEC菌株。32株DEC对氨苄西林、四环素、甲氨苄啶/磺胺异恶唑耐药率最高,分别为53.12%、43.75%和37.5%。ESBLs(+)株占比18.75%,其多重耐药菌株检出率为83.83%,显著高于ESBLs(-)菌株,差异有统计学意义(P=0.042)。32株DEC共有25个ST型,优势基因型为ST10共4株(12.5%),ST28、ST31和ST3153各2株(各占比6.25%),其他21个型别各1株菌(各占比3.13%)。EAEC中检出1株携带bla_(NDM-1)的耐碳青霉烯类菌株。结论我院感染性腹泻患者的DEC以aggR、pic、astA、eae 4种毒力基因较为常见,以EAEC、EPEC为主要型别,基因型别呈高度多态性,检出多重耐药菌株。

关键词：致泻性大肠埃希菌毒力基因耐药基因

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Lactate anions participate in T cell cytokine production and function

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Science China(Life Sciences) 2021年第11期64卷 1895-1905页

作者： Jing Wen Shipeng Cheng Yaguang Zhang Ran Wang Jiefang Xu Zhiyang Ling liyan ma Xinghao Ai Bing Sun School of Life Science and Technology ShanghaiTech UniversityShanghai 201210China State Key Laboratory of Cell Biology CAS Center for Excellence in Molecular Cell ScienceShanghai Institute of Biochemistry and Cell BiologyChinese Academy of SciencesShanghai 200031China School of Life Science University of Science and Technology of ChinaHefei 230026China Shanghai Lung Cancer Center Shanghai Chest HospitalShanghai Jiao Tong UniversityShanghai 200030China University of Chinese Academy of Sciences Beijing 100049China

After antigen stimulation, T cells preferentially increase aerobic glycolysis to meet the bioenergetic and biosynthetic demands of T cell activation, proliferation, and effector functions. Lactate, a by-product of glycolysis, has been reported to function as an important energy source and signaling molecule. Here, we found that lactate anions are involved in cytokine production in Tcells after TCR activation. During ex vivo T cell activation, the addition of excess sodium lactate(Na L) increased the production of cytokines(such as IFNγ/IL-2/TNFα) more than the addition of sodium chloride(NaCl). This enhanced cytokine production was dependent on TCR/CD3 activation but not CD28 activation. In vivo, Na L treatment inhibited tumour growth in subcutaneously transplanted tumour models in a T cell-dependent manner, which was consistent with increased T cell cytokine production in the Na L treatment group compared to the Na Cl treatment group. Furthermore, a mechanistic experiment showed that this enhanced cytokine production was regulated by GAPDH-mediated post-transcriptional regulation. Taken together, our findings indicate a new regulatory mechanism involved in glycolysis that promotes T cell function.

关键词： lactate glycolysis TCR activation immunometabolism

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