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Gene regulation of anthocyanin biosynthesis in two blood-flesh peach （Prunus persica （L.） Batsch） cultivars during fruit development

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2014年第9期15卷 809-819页

作者： Yun JIAO Rui-juan ma Zhi-jun SHEN juan YAN Ming-liang YU Jiangsu Key Laboratory for Horticultural Crop Genetic Improvement Institute of HorticultureJiangsu Academy of Agricultural Sciences

The blood-flesh peach has become popular in China due to its attractive anthocyanin-induced pigmenta- tion and antioxidant properties. In this study, we investigated the molecular mechanisms underlying anthocyanin accumulation by examining the expression of nine genes of the anthocyanin biosynthesis pathway found in the peach mesocarp. Expression was measured at six developmental stages in fruit of two blood-flesh and one white-flesh peach cultivars, using quantitative reverse transcription polymerase chain reaction （qRT-PCR）. Results show that the expression of the chalcone synthase （CHS） gene was closely related to anthocyanin accumulation in both of the blood-flesh peaches. In the white-flesh peach, we found that the transcription level of phenylalanine ammonia-lyase （PAL） during fruit development was much lower than that in the blood-flesh peach, even though all other genes of the anthocyanin biosynthesis pathway were highly expressed, suggesting that the PAL gene may be limiting in antho- cyanin production in the white-flesh peach. Moreover, the transcription levels of the CHS and UDP-glucose-flavonoid 3-O-glucosyltransferase （UFGT） genes were markedly up-regulated at three days after bag removal （DABR） in the blood-flesh peach, suggesting that CHS and UFGT are the key genes in the process of anthocyanin biosynthesis for both of the blood-flesh peaches. The present study will be of great help in improving understanding of the molecular mechanisms involved in anthocyanin accumulation in blood-flesh peaches.

关键词： Blood-flesh peach Anthocyanins Bagging Gene expression

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The Herbicidal Activity of Mutant Isolates from Botrytis cinerea

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Agricultural Sciences in China 2006年第8期5卷 622-628页

作者： ZHANG Jin-lin ZHANG Li-hui LIU Ying-chao ma juan LI Chuan DONG Jin-gao Mycotoxin Laboratory Hebei Agricultural University Baoding 071001 P.R.China

Fifteen mutant isolates were obtained by ultraviolet mutation from parent isolate Botrytis cinerea BC-4. Among them three mutant isolates, BC4-1, BC4-2, and BC4-15, showed strong herbicidal activity. BC4-1 showed maximum herbicidal activity for inhibition of germination and growth of Digitaria sanguinalis L. and Arnaranthus retroflexus L. The results also showed that herbicidal activity was influenced by differing pH of PD media, with pH value of 4.0 being the optimum. The crude toxin was extracted using chloroform, petroleum ether, and ethyl acetate, respectively, and the ethyl acetate extracts showed the strongest inhibitory activity on the germination and growth of D. sanguinalis L. and A. retroflexus L. Using HPLC, one fraction with an absorption peak at 271 nm was separated from the crude toxin. This fraction could strongly inhibit the growth of D. sanguinalis L. at a concentration of 100 mg L^-1 and could completely inhibit the seed germination of D. sanguinalis L. and A. retroflexus L. at a concentration of 50 mg L^-1 .

关键词： Botrytis cinerea toxin ultraviolet mutation herbicide activity separation and purification

Indoleamine-2,3-dioxygenase 1/cyclooxygenase 2 expression prediction for adverse prognosis in colorectal cancer

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World Journal of Gastroenterology 2018年第20期24卷 2181-2190页

作者： Wen-juan ma Xing Wang Wen-Ting Yan Zhong-Guo Zhou Zhi-Zhong Pan Gong Chen Rong-Xin Zhang State Key Laboratory of Oncology in South China Sun Yat-sen UniversityGuangzhou 510060Guangdong ProvinceChina Collaborative Innovation Center of Cancer Medicine Guangzhou 510060Guangdong ProvinceChina Tongji Medical College Huazhong University of Science and TechnologyWuhan 430074Hubei ProvinceChina Department of Hepatobiliary Surgery Cancer CenterSun Yat-sen UniversityGuangzhou 510060Guangdong ProvinceChina Department of Colorectal Surgery Cancer CenterSun Yat-sen UniversityGuangzhou 510060Guangdong ProvinceChina

AIM To evaluate indoleamine-2,3-dioxygenase 1/cyclooxygenase 2(IDO1/COX2) expression as an independent prognostic biomarker for colorectal cancer(CRC) *** We retrospectively studied the medical records of 95 patients who received surgical resection from August 2008 to January 2010. All patients were randomly assigned to adjuvant treatment with or without celecoxib groups after surgery. We performed standard immunohistochemistry to assess the expression levels of IDO1/COX2 and evaluated the correlation of IDO1/COX2 with clinicopathological factors and overall survival(OS) *** The expression of nuclear IDO1 was significantly correlated with body mass index(P < 0.001), and IDO1 expression displayed no association with sex, age, tumor differentiation, T stage, N stage, carcinoembryonic antigen, cancer antigen 19-9, CD3+ and CD8+ tumor infiltrating lymphocytes, and COX2. In univariate analysis, we found that nuclear IDO1(P = 0.039), nuclear/cytoplasmic IDO1 [hazard ratio(HR) = 2.044, 95% confidence interval(CI): 0.871-4.798, P = 0.039], nuclear IDO1/COX2(HR = 3.048, 95%CI: 0.868-10.7, P = 0.0049) and cytoplasmic IDO1/COX2(HR = 2.109, 95%CI: 0.976-4.558, P = 0.022) all yielded significantly poor OS outcomes. Nuclear IDO1(P = 0.041), nuclear/cytoplasmic IDO1(HR = 3.023, 95%CI: 0.585-15.61, P = 0.041) and cytoplasmic IDO1/COX2(HR = 2.740, 95%CI: 0.764-9.831, P = 0.038) have significantly poor OS outcomes for the CRC celecoxib subgroup. In our multivariate Cox model, high coexpression of cytoplasmic IDO1/COX2 was found to be an independent predictor of poor outcome in CRC(HR = 2.218, 95%CI: 1.011-4.48, P = 0.047) and celecoxib subgroup patients(HR = 3.210, 95%CI: 1.074-9.590, P = 0.037).CONCLUSION Our results showed that cytoplasmic IDO1/COX2 coexpression could be used as an independent poor predictor for OS in CRC.

关键词： Prognosis Indoleamine-2,3-dioxygenase 1 Cyclooxygenase 2 Colorectal cancer

HDAC-selective Inhibitor Cay10603 Has Single Anti-tumour Effect in Burkitfs Lymphoma Cells by Impeding the Cell Cycle

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Current Medical Science 2019年第2期39卷 228-236页

作者： Xiu-juan ma Gang XU Zhi-jie LI Fang CHEN Di WU Jia-ning MIAO Yue ZHAN Yang FAN Medical Research Center Shengjing HospitalChina Medical UniversityShenyang 110004China Department of Pediatrics Shengjing HospitalChina Medical UniversityShenyang 110004China Department of Hematology Laboratoiy Shengjing HospitalChina Medical UniversityShenyang 110004China

Histone deacetylases(HDACs)inhibitors are novel in cancer therapy ***6-selective inhibitors exert advantageous effects due to higher selectivity and less *** explored the anti-tumor effect and the molecular mechanism of cay 10603,a potent HDAC6 inhibitor in Burkitt's lymphoma *** study revealed cay 10603 inhibited the proliferation of Burkitt's lymphoma cell lines,and induced caspase-dependent ***0603 inhibited the expression of CDKs and cyciins to impede cell cycle progression in both Burkitt's lymphoma cell *** 10603 also showed the additive effect with vpl 6 *** data presented the promising anti-tumor effect of cay 10603 in the Burkitt's lymphoma therapy.

关键词： Burkitt's lymphoma HDAC6 cell cycle apoptosis

Researches on chemically modified electrode *** reduction of oxygen on iron porphyrin modified electrode

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Chinese Journal of Chemistry 1984年第2期2卷 95-104页

作者： DONG Shao-Jun XU Li-juan ma Yue Changchun Institute of Applied Chemistry,Academia Sinica Changchun Institute of Applied Chemistry Academia Sinica

By surface organic synthesis a Fe(Ⅲ)tetra-o-aminophenyl porphyrin modified electrodewas successfully prepared on glassy carbon electrode surface through *** preparativeconditions were investigated in *** cyclic voltammetry and semidifferential neo-polarogra-phy in an acidic aqueous solution the behavior of the catalytic reduction ofoxygen on iron porphyriamodified electrode was *** on experimental results it was shown that the irreversibleprocess of 2-electron-reduction to hydrogen peroxide belongs to EC parallel catalytic process.

关键词： TAPP Researches on chemically modified electrode V.Catalytic reduction of oxygen on iron porphyrin modified electrode

Antioxidant and Anti-inflammatory Capacity of Ferulic Acid Released from Wheat Bran by Solid-state Fermentation of Aspergillus niger

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Biomedical and Environmental Sciences 2019年第1期32卷 11-21页

作者： YIN Zhi Na WU Wen Jia SUN Chong Zhen LIU Hui Fan CHEN Wen Bo ZHAN Qi Ping LEI Zhuo Gui XIN Xuan ma juan juan YAO Kun MIN Tian ZHANG Meng Meng WU Hui Biotechnology guangdong polytechnic science and trade School of Medical Technology and Nursing Shenzhen Polytechnic College of Food Science and Engineering South China University of Technology

Objective A strain of Aspergillus niger(A. niger), capable of releasing bound phenolic acids from wheat bran, was isolated. This strain was identified by gene sequence identification. The antioxidant and anti-inflammatory capacity of ferulic acid released from wheat bran by this A. niger strain(FA-WB) were evaluated. Methods Molecular identification techniques based on PCR analysis of specific genomic sequences were conducted; antioxidant ability was examined using oxygen radical absorbance capacity(ORAC), cellular antioxidant activity(CAA) assays, and erythrocyte hemolysis assays. RAW264.7 cells were used as a model to detect anti-inflammatory activity. Results The filamentous fungal isolate was identified to be A. niger. ORAC and CAA assay showed that FA-WB had better antioxidant activity than that of the ferulic acid standard. The erythrocyte hemolysis assay results suggested that FA-WB could attenuate AAPH-induced oxidative stress through inhibition of reactive oxy gen species(ROS) generation. FA-WB could significantly restore the AAPH-induced increase in intracellular antioxidant enzyme activities to normal levels as well as inhibit the intracellular malondialdehyde formation. TNF-?, IL-6, and NO levels indicated that FA-WB can inhibit the inflammation induced by lipopolysaccharide(LPS). Conclusion Ferulic acid released from wheat bran by a new strain of A. niger had good anti-inflammatory activity and better antioxidant ability than standard ferulic acid.

关键词： Fungal identification Fermentation Ferulic acid Antioxidant activity Oxidative hemolysis Anti-inflammatory activity.

含有界随机参数的双势阱Duffing-van der Pol系统的倍周期分岔

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物理学报 2006年第2期55卷 610-616页

作者：孙晓娟徐伟马少娟西北工业大学应用数学系西安710072

讨论简谐激励作用下含有界随机参数的双势阱Duffing-van der Pol系统的倍周期分岔现象.首先用Chebyshev多项式逼近法将随机Duffing-van der Pol系统化成与其等价的确定性系统,然后通过等价确定性系统来探索该系统的倍周期分岔现象.数值... 详细信息

讨论简谐激励作用下含有界随机参数的双势阱Duffing-van der Pol系统的倍周期分岔现象.首先用Chebyshev多项式逼近法将随机Duffing-van der Pol系统化成与其等价的确定性系统,然后通过等价确定性系统来探索该系统的倍周期分岔现象.数值模拟显示随机Duffing-van der Pol系统与均值参数系统有着类似的倍周期分岔行为,同时指出,随机参数系统的倍周期分岔有其自身独有的特点.文中的主要数值结果表明Chebyshev多项式逼近法是研究非线性随机参数系统动力学问题的一种有效方法.

关键词： Chebyshev多项式随机Duffing—van der Pol系统倍周期分岔

Identification of Boseongicola sediminum ***.,a Novel Decabromodiphenyl Ether(BDE-209)-tolerant Strain Isolated from Coastal Sediment in Xiamen,China

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Biomedical and Environmental Sciences 2021年第8期34卷 656-661页

作者： TANG Chen FAN Chun GUO Dong Bei ma Xiu juan CAI Qing Tao CHEN Xiao Xuan ZHANG Min LI Jia Yao AN Qiu Ying ZHAO Ran State Key Laboratory of Molecular Vaccinology and Molecular Diagnotics School of Public HealthXiamen UniversityXiamen 361102FujianChina Department of Environ mental and Occupational Medicine Shanghai Jinshan Disease Center for Prevention and ControlShanghai 201599China Department of Environmental and Occupational Health Huzhou Center for Disease Prevention and ControlHuzhou 313000ZhejiangChina

Polybrominated diphenyl ethers(PBDEs)are a group of aromatic organic bromine compounds,which have been used in a broad array of polymeric materials(plastics,foams,resins,and adhesives)as brominated flame *** are used in commercial and household products,including textiles,electronic equipment,airplanes,and automobiles,especially in China and the USA,because they are inexpensive with excellent flame-retardant effects[1].During the past decade,over 14,000 tonnes of PBDEs have entered the market,and have become ubiquitous environmental contaminants[2].

关键词： Xiamen resins aromatic

Panaxadiol inhibits programmed cell deathligand 1 expression and tumor proliferation via hypoxia-inducible factor(HIF)-1a and STAT3 in human colon cancer cells

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中国药理学与毒理学杂志 2019年第10期 888-889页

作者： WANG Zhe LI Ming-yue ZHANG Zhi-hong ma juan JIN Xue-jun Molecular Medicine Research Center Colege of PharmacyYanbian University

OBJECTIVE To investigate the potential anti-cancer activity of panaxadiol on PD-L1 expression and tumor proliferation in human colon cancer cells and to identify the underlying mechanism. METHODS HCT116, SW620, and HT29 cells were pretreated with panaxadiol(1, 3, 10, 30 μmol·L-1) for 48 h to verify cell viability by MTT assay. HCT-116 cells were pretreated with panaxadiol(1, 3, 10 μmol·L-1) for 12 h in Western blotting, flow cytometry analysis, and reverse transcription-PCR（RTPCR）. The interaction between panaxadiol and HIF-1α/STAT3 was studied by molecular docking and immunofluorescence. HCT-116/T-cell coculture model and T-cell killing assay were employed to investigated the immunosuppressive of HCT-116 cells. Colony cormation and Ed U labeling assay were performed to detect cell proliferation. Panaxadiol at a dose of 10 and 30 mg·kg-1were orally administered in xenografted assay and Immunohistochemical analysis. RESULTS Panaxadiol showed little cytotoxicity according to the result of MTT assay and exerted a potent inhibitory activity against PD-L1 expression both on cell surface and protein/m RNA level in a dose-dependent manner. It was subsequently shown that panaxadiol inhibited STAT3 activation through JAK1,JAK2, and Src pathways. Simultaneously panaxadiol promoted degradation and depressed synthesis of HIF-αprotein induced by hypoxia. Moreover, pretrement of panaxadiol could restore the activity of cytotoxic T lymphocyte（CTL） and regain their capacity of tumor cell killing in a T cell/tumor cell co-culture system. Colony formation and Ed U labeling assays Further demonstrated that panaxadiol inhibited tumor proliferation. The Proliferation inhibitory effect in vivo of panaxadiol was also proved by xenograft assay. CONCLUSION Taken together, these studies reveal previously unknown characteristics of panaxadiol provide new perspectives into the mechanism of cancer inhibition of the compound.

关键词： panaxadiol PD-L1 HIF-1α STAT3 proliferation