检索结果-南通市图书馆

A practical guide for fast implementation of SNARE-mediated liposome fusion

Biophysics Reports 2024年第1期10卷 31-40页

作者： shen Wang Cong ma Key Laboratory of Molecular Biophysics of the Ministry of Education College of Life Science and TechnologyHuazhong University of Science and Technology

Soluble N-ethylmaleimide-sensitive factor attachment protein receptor(SNAER) family proteins are the engines of most intra-cellular and exocytotic membrane fusion pathways(Jahn and Scheller 2006).Over the past two decades, in-vitro liposome fusion has been proven to be a powerful tool to reconstruct physiological SNARE-mediated membrane fusion processes(Liu et al. 2017). The reconstitution of the membrane fusion process not only provides direct evidence of the capability of the cognate SNARE complex in driving membrane fusion but also allows researchers to study the functional mechanisms of regulatory proteins in related pathways(Wickner and Rizo 2017). Heretofore, a variety of delicate methods for in-vitro SNARE-mediated liposome fusion have been established(Bao et al. 2018;Diao et al. 2012; Duzgunes 2003; Gong et al. 2015; Heo et al. 2021; Kiessling et al. 2015; Kreye et al.2008; Kyoung et al. 2013; Liu et al. 2017; Scott et al. 2003). Although technological advances have made reconstitution more physiologically relevant, increasingly elaborate experimental procedures, instruments, and data processing algorithms nevertheless hinder the non-experts from setting up basic SNARE-mediated liposome fusion assays. Here, we describe a low-cost, timesaving, and easy-to-handle protocol to set up a foundational in-vitro SNARE-mediated liposome fusion assay based on our previous publications(Liu et al. 2023; Wang and ma 2022). The protocol can be readily adapted to assess various types of SNARE-mediated membrane fusion and the actions of fusion regulators by using appropriate alternative additives(e.g., proteins, macromolecules, chemicals, etc.). The total time required for one round of the assay is typically two days and could be extremely compressed into one day.

关键词： Liposome fusion SNARE In-vitro reconstitution Protocol

来源：

同方期刊数据库评论

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Study of factors influencing the occurrence rate of 60 Hz power line radiation in the topside ionosphere:A systematic survey using the CSES satellite

引用

Science China(Technological Sciences) 2024年第6期67卷 1879-1892页

作者： ma ZhenHui ZHAO ShuFan LIAO Li shen XuHui LU HengXin School of Emergency management Science and Engineering University of Chinese Academy of SciencesBeijing100049China State Key Laboratory of Space Weather National Space Science CenterChinese Academy of SciencesBeijing100190China Key Laboratory of Solar Activity and Space Weather National Space Science CenterChinese Academy of SciencesBeijing100190China National Institute of Natural Hazards Ministry of Emergency Management of ChinaBeijing100085China Institute of Geophysics China Earthquake AdministrationBeijing100081China

We analyze the topside ionosphere power line radiation(PLR)at 60 Hz over the US using electric field data collected by CSES satellite between January 2019 and December *** study aimed to further investigate the month-to-month variation characteristic of PLR occurrence rate observed by satellite and its several influencing factors,including solar radiation,lightning activity,and try to clarify the influence of electricity *** results show that the solar radiation(solar zenith angle and F10.7)plays a major role in the variation of the PLR occurrence rate,and that there is no direct connection with the number of *** the relationship between PLR occurrence rate and electricity consumption,the low occurrence rate associated with decreased weekend electricity consumption was not observed in the ***,there is a significant difference in PLR occurrence rate between the East and West Coasts of the US at the same latitude during the same time period,suggesting that the significant difference in PLR occurrence rate is caused by the significant difference in electricity consumption between the two *** excluding the effect of solar radiation on PLR occurrence rate,we concluded that only a significant difference in regional electricity consumption could lead to a corresponding change in PLR occurrence rate detected by the Low-Earth-Orbit ***,we also found there is seasonal variation in the diurnal differences of the PLR occurrence rate caused by seasonal variation of the lower ionosphere.

关键词： CSES satellite power line radiation occurrence rate solar radiation lightning activity electricity consumption

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

A Study on the Contribution of Different Food Sources to Shrimp Growth in an Intensive Fenneropenaeus chinensis Pond

引用

Journal of Ocean University of China 2008年第4期7卷 453-456页

作者： SU Yuepeng ma shen TIAN Xiangli DONG Shuanglin The Key Laboratory of mariculture Ministry of Education College of Fisheries Ocean University of China Qingdao 266003 P R. China

Stable isotope methods can be used to determine the food sources and prey items of aquatic organisms accurately and reliably. This study examined the relative contribution of artificial foods （the formulated feed and Arternia） and natural foods to shrimp growth in an intensive Fenneropenaeus chinensis pond by using carbon and nitrogen stable isotopes. The results showed that the nutrition utilization efficiency of the harvested shrimp was low, only 33.18% of feed nitrogen and 21.73% of feed carbon being converted to shrimp flesh, Our stable isotope results showed that the shrimp obtained nutrition for maximum growth from artificial foods, whose contribution was 93.5%, with the remaining attributed to the natural foods. However, there was 0.94t harvested shrimp derived from natural foods （the rest of 13.56t harvested shrimp derived from artificial foods） in lha intensive pond with a shrimp production of 14.50 tha^-1. Therefore, unit area shrimp production can be increased by increasing the contribution proportion of natural foods in intensive shrimp farming.

关键词： shrimp pond nutrition utilization stable isotope contribution intensive

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Heterogeneous MXene-based films with graded electrical conductivity towards highly efficient EMI shielding and electrothermal heating

引用

Nano Research 2024年第8期17卷 7264-7274页

作者： Yu Zhang Guangcheng Zhang Zhonglei ma Jianbin Qin Xi shen Department of Aeronautical and Aviation Engineering The Hong Kong Polytechnic UniversityHung HomKowloonHong KongChina Shaanxi Key Laboratory of macromolecular Science and Technology School of Chemistry and Chemical EngineeringNorthwestern Polytechnical UniversityXi’an 710072China Research Institute for Sports Science and Technology The Hong Kong Polytechnic UniversityHung HomKowloonHong KongChina

The increasing need for electromagnetic interference(EMI)shielding of electronics in cold environments such as those in aircraft,space exploration,and wearable heaters to avoid hazardous icing conditions or hypothermia requires the development of thin and lightweight EMI shielding materials preferably by absorbing rather than reflecting electromagnetic(EM)waves while also generating heat through energy-efficient electrothermal ***,it is challenging to achieve absorption-dominant EMI shielding and energy-efficient electrothermal heating simultaneously in a thin and lightweight ***,we develop a heterogeneous composite film comprising a porous multi-walled carbon nanotubes(MWCNTs)/bacterial cellulose(BC)film and an aligned MXene/Ag nanowires(NWs)backing via a sequential vacuum filtration *** porous film contains random conductive networks of MWCNTs with moderate conductivity while the aligned MXene sheets atop Ag NWs network affords high conductivity in the backing,giving rise to graded electrical conductivity for absorption-dominant EMI *** increasing Ag NW coverage leads to significantly increased electrical conductivity without increasing the EM wave reflection as well as the density and thickness of the film,yielding excellent specific EMI shielding effectiveness(>8500 dB/(g·cm^(2))),low driving voltage for energy-efficient electrothermal heating(163℃at 2.5 V),and fast response time(60 s)at a low areal density of 0.015 mg/cm^(2).Combining EMI shielding and electrothermal heating,the heterogeneous film developed here are promising contenders for the protection of electronic equipment in low-temperature environment.

关键词： heterogenous composite film MXene electromagnetic interference(EMI)shielding thermal management

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Impact of High Sodium Diet on Neovascularization and Osseointegration around Titanium Implant:An in Vivo and in Vitro Study

引用

Biomedical and Environmental Sciences 2024年第7期37卷 739-753页

作者： Keyuan Xu Xiaoting Tang Yun Xiang Yiding shen Zhennan Deng Pingping ma Xinkun shen Department of Orthopaedics The Third Affiliated Hospital of Wenzhou Medical University(Ruian People’s Hospital)Wenzhou 325016ZhejiangChina School and Hospital of Stomatology Wenzhou Medical UniversityWenzhou 325027ZhejiangChina

Objective A high sodium(HS)diet is believed to affect bone metabolism *** its impact on osseointegration of titanium(Ti)implants holds significant implications for postoperative dietary management of implanted *** This investigation probed the impact of sodium ions(Na^(+))on neovascularization and osteogenesis around Ti implants in vivo,utilizing micro-computed tomography,hematoxylin and eosin staining,and immunohistochemical ***,in vitro experiments assessed the effects of varied Na^(+)concentrations and exposure durations on human umbilical vein endothelial cells(HUVECs)and MC3T3-E1 *** In vivo,increased dietary sodium(0.8%-6.0%)led to a substantial decline in CD34 positive HUVECs and new bone formation around Ti implants,alongside an increase in inflammatory *** vitro,an increase in Na^(+)concentration(140-150 mmol/L)adversely affected the proliferation,angiogenesis,and migration of HUVECs,especially with prolonged *** MC3T3-E1 cells initially exhibited less susceptibility to high Na^(+)concentrations compared to HUVECs during short-term exposure,prolonged exposure to a HS environment progressively diminished their proliferation,differentiation,and osteogenic *** These findings suggest that HS diet had a negative effect on the early osseointegration of Ti implants by interfering with the process of postoperative vascularized bone regeneration.

关键词： High-sodium Implants Vascularization Osseointegration

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Influence of Probiotics on the Growth and Digestive Enzyme Activity of White Pacific Shrimp (Litopenaeus vannamei)

引用

Journal of Ocean University of China 2008年第2期7卷 215-218页

作者： Gómez R. Geovanny D. ma shen College of Fisheries Ocean University of China College of Fisheries Ocean University of China Qingdao 266003 P. R. China

The influence of Bacillus probiotics on the digestive enzyme activity and the growth of Litopenaeus vannamei were de- termined in this study. The shrimp was treated with five percentages (1.5, 3.0, 4.5, 6.0 and 7.5) of probiotics (Bacillus spp.) supple- mented to the feed and cultured for 45 d. The growth measured as the weight gain at the end of culturing was significantly (P<0.05) higher in probiotic-treated shrimps than that of the control (without receiving probiotics). Activities of protease and amylase, two digestive enzymes of the midgut gland and the intestine were significantly (P<0.05) higher in probiotic-treated shrimp than in the control.

关键词： Bacillus digestive enzymes growth midgut gland probiotic

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Influence of Thermal Homogenization Treatment on Structure and Impact Toughness of H13 ESR Steel

引用

Journal of Iron and Steel Research International 2009年第5期16卷 56-60页

作者： ma Dang-shen ZHOU Jian CHEN Zai-zhi ZHANG Zhong-kan CHEN Qi-an LI De-hui Central Iron and Steel Research Institute Beijing 100081 China Jiangsu Tiangong Tool Co Ltd Danyang 212312 Jiangsu China

The as-cast microstructure of H13 ESR ingot and the influence of high temperature diffusion treatment on the structure and impact toughness have been investigated. The results show that the dendrite arm spacing gradually becomes wide from the surface to the center of ingot, and the large primary carbide particles always exist in interdendritic segregation areas; by means of high temperature diffusion treatment of ingot prior to hot forging, the banded segregation is nearly eliminated, the annealed structure is more uniform and the isotropic properties have been improved remarkably.

关键词： H13 microstructure homogenization impact toughness isotropy

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Multi-scale cross-domain alignment for person image generation

引用

CAAI Transactions on Intelligence Technology 2024年第2期9卷 374-387页

作者： Liyuan ma Tingwei Gao Haibin shen Kejie Huang Department of Information Science&Electronic Engineering Zhejiang UniversityHangzhouZhejiangChina Alibaba Group HangzhouChina

Person image generation aims to generate images that maintain the original human appearance in different target *** works have revealed that the critical element in achieving this task is the alignment of appearance domain and pose *** alignment methods,such as appearance flow warping,correspondence learning and cross attention,often encounter challenges when it comes to producing fine texture *** approaches suffer from limitations in accurately estimating appearance flows due to the lack of global receptive ***,they can only perform cross-domain alignment on high-level feature maps with small spatial dimensions since the computational complexity increases quadratically with larger feature *** this article,the significance of multi-scale alignment,in both low-level and high-level domains,for ensuring reliable cross-domain alignment of appearance and pose is *** this end,a novel and effective method,named Multi-scale Crossdomain Alignment(MCA)is ***,MCA adopts global context aggregation transformer to model multi-scale interaction between pose and appearance inputs,which employs pair-wise window-based cross ***,leveraging the integrated global source information for each target position,MCA applies flexible flow prediction head and point correlation to effectively conduct warping and fusing for final transformed person image *** proposed MCA achieves superior performance on two popular datasets than other methods,which verifies the effectiveness of our approach.

关键词： artificial intelligence image processing image reconstruction

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Growth,optical dispersion and magnetic behavior of Dy^(3+) doped yttrium iron garnet crystals

引用

Journal of Rare Earths 2024年第6期42卷 1110-1117,I0004页

作者： Yating Fang Hui shen Yudie ma Shijie Liao Shuang Xia Tian Tian Ding Zhou Yunfeng ma Jiayue Xu Institute of Crystal Growth School of Materials Science and EngineeringShanghai Institute of Technology National Engineering Center of Electromagnetic Radiation Control materials School of Electronic Science and EngineeringUniversity of Electronic Science and Technology of China

Y_(3)Fe_(5)O_(12)(YIG)crystals are highly desirable for the fabrication of mid-infrared isolators with the rapid growth of optical communications,although it is rather challenging to grow large size and high-quality single ***^(3+)doping is expected to improve the optical and magneto-optical prope ***,high quality Dy:YIG crystals and the adjustment of Dy^(3+)on the structure and optical behavior of YIG crystal remain unclear,impeding its practical ***,a series of Y_(3-x)Dy_(x)Fe_(5)O_(12)(x=0,0.5,1.0,1.5,3)solid solution crystals was grown by the flux-Bridgman method and single crystals up to 25 mm were *** the introduction of Dy^(3+),lattice parameters are gradually enlarged from 1.2379 nm(YIG)to 1.2420 nm(DyIG).Typical Dy^(3+)absorption peaks are observed around 1070,1265 and 1670 *** refractive index decreases from 2.37(500 nm)to 2.10(2500 nm)for YIG crystal,and it reduces from 2.47(500 nm)to 2.16(2500 nm)for DyIG *** optical bandgaps remain almost unchanged for Dy:YIG *** optical dispersion of the refractive indices was finely fitted by the Wemple and DiDomenico(WDD)and the Sellmeier models,*** the increase of Dy^(3+)content,the saturation magnetization(Ms)decreases significantly from 23.62 emu/g(YIG)to 5.33 emu/g(DyIG).Small coercive field is persisted for this system,endowing small external magnetic *** results provide valuable references for the manipulation of rare earths on the properties of magnetooptical crystals,which is beneficial to the design of high-performance garnet crystals for the application of optical switching and non-reciprocal related devices.

关键词： magneto-optical crystal Yttrium iron garnet Dy^(3+)doping Crystal growth Optical dispersion Rare earths

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Association analysis of BclI with benign lymphoepithelial lesions of the lacrimal gland and glucocorticoids resistance

引用

International Journal of Ophthalmology(English edition) 2023年第11期16卷 1734-1745页

作者： Xu-Juan Zhang Peng-Xiang Zhao Ming-shen ma Hao Wu Rui Liu Hui Wang Meng-Yu Liu Fei Xie Xue-Mei ma Faculty of Environment and Life Beijing University of TechnologyBeijing 100124China Beijing International Science and Technology Cooperation Base of Antivirus Drug Beijing 100124China Beijing Tongren Hospital Capital Medical UniversityBeijing 100730China State Key Laboratory of Military Stomatology&National Clinical Research Center for Oral Diseases&Shaanxi International Joint Research Center for Oral Diseases Department of General Dentistry and EmergencySchool of StomatologyAir Force MedicalXi’an 710032Shaanxi ProvinceChina

AIM:To evaluate the relationship between gene polymorphism(BclI,ER22/23EK,N363S)and the occurrence,progression and sensitivity to glucocorticoid of lacrimal gland benign lymphoepithelial lesion(LGBLEL).METHODS:Clinical peripheral blood samples of 52 LGBLEL patients and 10 normal volunteers were collected for DNA extraction and polymerase chain reaction sequencing to analyze single nucleotide polymorphism(SNP)*** lacrimal tissues of LGBLEL were surgically removed and made into paraffin sections for subsequent hematoxylin-eosin(HE)and masson staining *** duration of disease and hormone use of LGBLEL patients from diagnosis to surgery were also *** Meta-analysis follows PRISma guidelines to conducted a systematic review of human studies investigating the relationship between the NR3C1 BclI polymorphism and glucocorticoids(GCs)***:There was no association between ER22/23EK or N363S and the occurrence of LGBLEL or GCs sensitivity(P>0.05);BclI GC genotype was closely related to GCs resistance(P=0.03)as is the minor allele C(P=0.0017).The HE staining and masson staining showed that the GC genotype of BclI remarkably slowed down the disease progression and reduced fibrosis(Pmay be more sensitive to GCs and associated with lower fibrosis and slower disease *** results may guide the clinical treatment strategy for the LGBLEL patients.

关键词： lacrimal gland benign lymphoepithelial lesion Bcll single nucleotide polymorphisms glucocorticoids resistance fibrosis

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：