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Purple acid phosphatase promoted hydrolysis of organophosphate pesticides in microalgae

environmental Science and Ecotechnology 2024年第2期18卷 137-149页

作者： Xiang Wang Guo-Hui He Zhen-Yao Wang Hui-Ying Xu Jin-Hua Mou Zi-Hao Qin Carol Sze Ki Lin Wei-Dong Yang Yalei Zhang Hong-ye Li Key Laboratory of Eutrophication and Red Tide Prevention of Guangdong Higher Education Institutes College of Life Science and TechnologyJinan UniversityGuangzhou510632China school of Energy and Environment City University of Hong KongTat Chee AvenueKowloonHong Kong Centre for Technology in Water and Wastewater School of Civil and Environmental EngineeringUniversity of Technology SydneyUltimoNSW2007Australia Southern marine Science and Engineering Guangdong Laboratory(Guangzhou) Guangzhou510000China State Key Laboratory of Pollution Control and resources Reuse College of Environmental Science and EngineeringTongji UniversityShanghai200092China

When organophosphate pesticides(OPs)are not used and handled in accordance with the current rules and standards,it results in serious threats to the aquatic environment and human *** tricornutum is a prospective microalgae-based system for pollutant removal and carbon *** engineered ***,designated as the OE line(endogenously expressing purple acid phosphatase 1[PAP1]),can utilize organic phosphorus for cellular ***,the competencies and mechanisms of the microalgae-based system(namely the OE line of ***)for metabolizing OPs remain to be *** this study,the OE line exhibited the effective biodegradation competencies of 7{2.2.and 68.2.for 30 mg L^(-1)of dichlorvos and 50 mg L^(-1)of glyphosate,accompanied by synergistic accumulations of biomass(0.91 and 0.95 g L^(-1))and lipids(32.71%and 32.08%),***,the biodiesel properties of the lipids from the OE line manifested a high potential as an alternative feedstock for microalgae-based biofuel production.A plausible mechanism of OPs biodegraded by overexpressed PAP1 is that sufficient inorganic P for adenosine triphosphate and concurrent carbon flux for the reduced form of nicotinamide adenine dinucleotide phosphate biosynthesis,which improved the OP tolerance and biodegradation competencies by regulating the antioxidant system,delaying programmed cell death and accumulating lipids via the upregulation of related *** sum up,this study demonstrates a potential strategy using a genetically engineered strain of *** to remove high concentrations of OPs with the simultaneous production of biomass and biofuels,which might provide novel insights for microalgae-based pollutant biodegradation.

关键词： Biodegradation Lipid accumulation Organophosphate pesticide Phaeodactylum tricornutum Purple acid phosphatase

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祁连山东段金塔河流域层状地貌时代与成因探讨

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山地学报 2005年第2期23卷 129-135页

作者：高红山潘保田李吉均邬光剑李炳元业渝光兰州大学西部环境教育部重点实验室和地理科学系中国科学院青藏高原研究所中国科学院地理科学与资源研究所国土资源部青岛海洋地质研究所甘肃兰州73000 北京100085 北京100101 山东青岛2.6071

祁连山东段金塔河流域分布着两级夷平面(山顶面与主夷平面)、一级剥蚀面和多级河流阶地,它们是晚新生代青藏高原阶段性隆升的产物。通过电子自旋共振(Electronspinresonance ,简写为ESR)、热释光(Lumines cence ,简写为TL)、红外释光(In... 详细信息

祁连山东段金塔河流域分布着两级夷平面(山顶面与主夷平面)、一级剥蚀面和多级河流阶地,它们是晚新生代青藏高原阶段性隆升的产物。通过电子自旋共振(Electronspinresonance ,简写为ESR)、热释光(Lumines cence ,简写为TL)、红外释光(Infra redstimulatedluminescence ,简写为IRSL)和14.C等绝对测年手段并结合区域对比的研究表明,该区山顶面形成于老第三纪,主夷平面形成于中新世至上新世,剥蚀面解体于1 4.0}B P 左右。其后金塔河流域发育5～6级阶地,形成时代大致为1 2.4.0}B P 、0 78maB P 、0 14.0}B P 、0 0 6maB P 、0 0 3maB P 和0 0 1maB P 。结合河流阶地的形成年代、发育特征以及邻区阶地的发育模式研究表明,它们应是构造隆升以及气候变化双重作用下的产物

关键词：形成时代剥蚀面河流阶地金塔河祁连山东段

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Efficiency of sediment transport by flood and its control in the Lower yellow River

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Science China(Technological Sciences) 2004年第z1期47卷 173-185页

作者： NI Jinren1, LIU Xiaoyong2. LI Tianhong1, ZHAO ye’an3 & JIN Ling1 1. Department of environmental Engineering, Peking university The Key Laboratory of Water and Sedi- ment Sciences, Ministry of Education, Beijing 100871, China 2. General Institute of Water {2. and Hydropower Planning and Design, Ministry of Water Re- sources, Beijing 100011, China 3. The yellow River Conservancy Commission, Zhengzhou 4.0003, China 1. Department of environmental Engineering Peking University 100871 Beijing China 2. The Key Laboratory of Water and Sediment Sciences Ministry of Education 100871 Beijing China 3. General Institute of Water resources and Hydropower Planning and Design Ministry of Water Resources 100011 Beijing China 4. The yellow River Conservancy Commission 450003 Zhengzhou China

This paper presents the characteristics of sediment transport by flood in the Lower yellow River with the reach from Huayuankou to Gaocun, which is regarded as a typical braided pattern. The Artificial Neural Network Model on Water Use for Sediment Transport (WUST) by flood was established based on the measured data from 1980 to 1998. Consequently, simulations of controlling process of sediment transport by flood were made in terms of the control theory under different scenarios. According to the situation of sediment transport by flood in the Lower yellow River, Open-Loop control system and feedback control system were adopted in system design. In the Open-Loop control system, numerical simulations were made to reveal the relationship between average discharge of flood and the WUST with varying sediment concentrations. The results demonstrate that sediment concentration has significant influence on the controlling process of flood flow to WUST. It is practical and efficient to control WUST if sediment concentration is less than {4. kg/m3. In the feedback control system, controlling processes of sediment concentration and flood discharge for sediment transport were simulated respectively under given conditions, and it was found that sediment transport process could be controlled completely by sediment concentration and discharge at the inlet of the reach from Huayuankou to Gaocun. Using the same method, controlling processes of sediment transport by flood in other reaches in the Lower yellow River were also simulated. For the case of sediment concentration being {4. kg/m3, the optimized controlling discharge ranges from {4.90 to {4.00 m3/s in the lower reach of Huayuankou. This study is also of significance to flood control and flushing sediment in the Lower yellow River with proper operation modes of Xiaolangdi Reservoir.

关键词： the Lower yellow River, flood, sediment transport, efficiency, simulation, control.

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Underestimation of phosphorus fraction change in the supernatant after phosphorus adsorption onto iron oxides and iron oxide–natural organic matter complexes

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Journal of environmental Sciences 2017年第5期29卷 197-205页

作者： jinlong Yan tao Jiang Ying Yao Jun Wang Yuanli Cai Nelson W.Green Shiqiang Wei Chongqing Key Laboratory of Agricultural resources and Environrnent College of Resources and Environment Department of Environmental Science and Engineering Southwest University Chongcling 400716 China Department of Forest Ecology and management Swedish University of Agricultural Sciences Umea SE-90183 Sweden school of materials Science & Engineering Beijing Institute of Technology Beijing 100081 China College of Chemistry and Chemical Engineering Mianyang Normal University Mianyang 621000 China school of Chemical and Biomolecular Engineering Georgia Institute of Technology Atlanta CA 30332 USA

The phosphorus（P） fraction distribution and formation mechanism in the supernatant after P adsorption onto iron oxides and iron oxide-humic acid（HA） complexes were analyzed using the ultrafiltration method in this *** an initial P concentration of 2. mg/L（I =0.01 mol/L and pH = 7）,it was shown that the colloid（1 kDa-0.4. μm） component of P accounted for 10.6%,11.6%,6.5%,and 4.0%of remaining total P concentration in the supernatant after P adsorption onto ferrihydrite（FH）,goethite（GE）,ferrihydrite-humic acid complex（FH-HA）,goethite-humic acid complex（GE-HA）,*** 〈1 kDa component of P was still the predominant fraction in the supernatant,and underestimated colloidal P accounted for 2.2.,55.1%,4..5%,and 38.7%of P adsorption onto the solid surface of FH,FH-HA,GE and GE-HA,***,the colloid P could not be ***,it could be interpreted that Fe3＋ hydrolysis from the adsorbents followed by the formation of colloidal hydrous ferric oxide aggregates was the main mechanism for the formation of the colloid P in the *** colloidal adsorbent particles co-existing in the supernatant were another important reason for ***,dissolve organic matter dissolved from iron oxide-HA complexes could occupy large adsorption sites of colloidal iron causing less colloid P in the ***,we believe that the findings can provide a new way to deeply interpret the geochemical cycling of P,even when considering other contaminants such as organic pollutants,heavy metal ions,and arsenate at the sediment/soil-water interface in the real environment.

关键词： Phosphorus Fraction Ultrafiltration Natural organic matter Iron-DOM Iron oxide-humic acid complexes

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环渤海河流营养盐的向海输送及环境效应

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中国环境科学 2024年第1期44卷 178-192页

作者：孙策策刘军张爱军李梦露吴文涛臧家业冉祥滨自然资源部第一海洋研究所自然资源部海洋生态环境科学与技术重点实验室山东青岛266061 中国水产科学研究院黄海水产研究所农业农村部海洋渔业可持续发展重点实验室山东省渔业资源与生态环境重点实验室山东青岛266071 山东长岛近海渔业资源国家野外科学观测研究站山东烟台265800 崂山实验室海洋地质过程与环境功能实验室山东青岛266237 中国海洋大学海洋化学理论与工程技术教育部重点实验室山东青岛266100

对2.17年枯水期、2.19年丰水期、2.2.年丰水期和2.2.年丰水期环渤海2.条主要入海河流溶解态无机营养盐向海输送的浓度、比值和通量进行了分析.结果表明:环渤海河流溶解无机氮(DIN)浓度在世界河流中处于较高水平,硝酸盐(NO_(3)^(-))为DI... 详细信息

对2.17年枯水期、2.19年丰水期、2.2.年丰水期和2.2.年丰水期环渤海2.条主要入海河流溶解态无机营养盐向海输送的浓度、比值和通量进行了分析.结果表明:环渤海河流溶解无机氮(DIN)浓度在世界河流中处于较高水平,硝酸盐(NO_(3)^(-))为DIN的主要赋存形态,溶解硅(DSi)和溶解无机磷(DIP)浓度较低.环渤海河流每年向渤海输送约0.61Gmol氨盐(NH_(4.^(+))、0.2.Gmol亚硝酸盐(NO_(2.^(-))、4.85Gmol NO_(3)^(-)、0.02.mol DIP和3.2.Gmol DSi.环渤海小河流在环渤海河流营养盐的向海输送中扮演着重要作用,尤其是DIP的输送可以占入海河流总通量的80%以上;小河流单位流域面积DIN、DIP和DSi输送通量均高于黄河.与世界河流相比,环渤海河流向海输送的营养盐比例失衡,具有高氮磷比、高硅磷比和低硅氮比的特点,磷限制显著.环渤海河流营养盐向海输送和结构失衡可能会进一步影响到渤海的初级生产和生态环境演变,值得深入研究.

关键词：环渤海河流营养盐营养盐结构通量渤海

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草地利用方式对土壤线虫群落结构及代谢足迹的影响

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中国环境科学 2024年第6期44卷 3342-3352页

作者：武振丹卢俊艳马尚飞叶贺张雅玲杨殿林红梅内蒙古农业大学草原与资源环境学院内蒙古呼和浩特010018 内蒙古土壤质量与养分资源重点实验室农业生态安全与绿色发展内蒙古高等学校重点实验室内蒙古呼和浩特010018 中国农业科学院草原研究所内蒙古呼和浩特010010 农业农村部环境保护科研监测所天津300191

2.2.年8月在贝加尔针茅草甸草原刈割、自由放牧与围封(对照)样地进行土壤线虫采集,探究草地不同利用方式对土壤线虫群落结构特征及代谢足迹的影响.本研究共捕获土壤线虫15632.,隶属于55属,群落优势属为鹿角唇属(Cervidellus),垫咽属(Ty... 详细信息

2.2.年8月在贝加尔针茅草甸草原刈割、自由放牧与围封(对照)样地进行土壤线虫采集,探究草地不同利用方式对土壤线虫群落结构特征及代谢足迹的影响.本研究共捕获土壤线虫15632.,隶属于55属,群落优势属为鹿角唇属(Cervidellus),垫咽属(Tylencholaimus)和丝尾垫刃属(Filenchus),分别占总捕获量的7.05%,7.14.和6.18%.较围封生境,线虫总多度在自由放牧生境显著降低.从功能类群来看,3种生境下植食性线虫(33.84.~37.4.%)与c-p 2.群(38.78%~4..58%)相对多度占比最大,且均在刈割生境与自由放牧生境显著增加.从多样性与生态指数来看,相较于围封,自由放牧生境成熟度指数(MI)与富集指数(EI)显著下降,基础指数(BI)显著增加,表明在自由放牧生境线虫群落多样性及稳定性降低,而食物网抗干扰能力增强;刈割生境结构指数(SI)显著提高,刈割生境食物网连通性提高,食物链加长.且在不同草地利用方式下线虫通路比值(NCR)>0.5瓦斯乐思卡指数(WI)>1,说明草甸草原土壤矿化途径的主要承担者是食微线虫,有机质分解以细菌通道为主.从代谢足迹来看,刈割和自由放牧生境均降低了线虫复合代谢足迹(TNf)和功能代谢足迹,食物网处于结构化状态,围封生境显著增加了线虫群落的整体活性和碳流动,食物网处于较为稳定的代谢平衡状态.不同草地利用方式下地下生物量(BGB),植物个体数(P-N)和速效钾(AK)是影响线虫群落发展的主要环境因子.综上所述,土壤线虫群落结构在不同草地利用方式下差异显著,不同生境中环境因子的变化是驱动土壤线虫群落变化的主要因子.

关键词：土壤动物群落特征代谢足迹草甸草原草地利用方式

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Reliable and Efficient Agrobacterium tumefaciens-Mediated Genetic Transformation of Dianthus spiculifolius

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Horticultural Plant Journal 2020年第3期6卷 199-204页

作者： Jingang Wang Shiyue Liu Hongping ma ye tao Shuang Feng Shufang Gong Jinzhu Zhang Aimin Zhou College of Horticulture and Landscape Architecture Northeast Agricultural UniversityHarbin 150030China Key Laboratory of Saline-Alkali Vegetation Ecology Restoration Northeast Forestry UniversityMinistry of EducationHarbin 150040China College of Life Sciences Northeast Forestry UniversityHarbin 150040China

Dianthus spiculifolius is a perennial herbaceous flower with strong environmental adaptability and is an important ornamental ground cover *** this study,seeds of *** were used as explants for callus induction,adventitious bud differentiation,and rooting by adding different concentrations of 2.4.dichlorophenoxyacetic acid(2.4.D),6-benzyl aminopurine(6-BA),and naphthaleneacetic acid(NAA)to Murashige and Skoog *** calli generated were co-cultured with Agrobacterium tumefaciens EHA105 containing pBI12.-GUS or pBI12.-GFP plasmids for 30 min,and transgenic regenerated plants were obtained by kanamycin(30mg·L^−1)***-PCR confirmed the stable expression of the exogenous GUS and GFP genes in the ***β-glucuronidase(GUS)histochemical staining confirmed GUS gene expression in transgenic calli,adventitious buds,and regenerated plants of *** green fluorescent protein(GFP)visual analysis showed GFP gene expression in transgenic ***,subcellular localization analysis showed that the three organelle marker proteins were not only successfully expressed but also accurately localized to their corresponding organelles in *** callus *** results indicated a successful establishment of a reliable and efficient ***-mediated genetic transformation system,which will contribute to functional gene research and genetic improvement of ***.

关键词： Agrobacterium tumefaciens Dianthus spiculifolius Callus Tissue culture Plant transformation

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黑河中游张掖地区沙漠化土地动态变化

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山地学报 2005年第2期23卷 153-157页

作者：齐善忠王涛罗芳丁建亮山东师范大学人口、资源与环境学院中国科学院寒区旱区环境与工程研究所山东省淄博市第十七中学山东济南2.0014 甘肃兰州730000 山东淄博2.5000

在我国的西北干旱区,由于所处的自然地理位置生态环境脆弱,加之人类不合理的经济活动,使得该区土地沙漠化现象严重,生态环境恶化。位于黑河流域中游的张掖地区,是我国西北地区重要的商品粮生产基地。由于近年来人口、经济快速增长的压... 详细信息

在我国的西北干旱区,由于所处的自然地理位置生态环境脆弱,加之人类不合理的经济活动,使得该区土地沙漠化现象严重,生态环境恶化。位于黑河流域中游的张掖地区,是我国西北地区重要的商品粮生产基地。由于近年来人口、经济快速增长的压力和人类对土地资源不合理的开发利用,产生了土地沙漠化等一系列环境问题,而且土地沙漠化总体呈上升扩展的趋势,严重地阻碍了绿洲经济和社会的持续、稳定发展。以1994.和2.0 0 0年具有相同时相(秋季)的LandsatTM 4.3、2.段(分别赋予红、绿、蓝三种颜色)、分辨率为30m的标准假彩色合成卫星影像的两期TM影像为信息源,利用Erdas8 4.感图象处理软件、Arcinfo8 1和Arcview3 2.GIS软件,对黑河流域中游张掖地区的沙漠化土地进行了动态监测研究和分析。结果表明:研究区的沙漠化土地类型共有8类,分别为流动沙地(丘)、半固定沙地(丘)、固定沙地(丘)、风蚀残丘、戈壁、闯田、潜在沙漠化土地和非生物治沙工程地;张掖地区各类沙漠化土地发展和逆转同时存在,1994.2.0 0 0年沙漠化土地面积增加了6 4.{4.7km2.。其中,临泽、山丹、民乐和肃南4.的沙漠化土地面积增加了714.85km2.,而张掖市和高台县沙漠化土地面积减少了72.6 8km2.。从总体上来看,全区的各类沙漠化土地变化仍呈上升

关键词：张掖地区沙漠化土地动态变化地理信息系统(GIS)

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Air pollution characteristics and their relationship with emissions and meteorology in the Yangtze River Delta region during 2.14.2.16

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Journal of environmental Sciences 2019年第9期31卷 8-20页

作者： tao ma Fengkui Duan Kebin He Yu Qin Dan Tong Guannan Geng Xuyan Liu Hui Li Shuo Yang Siqi ye Beiyao Xu Qiang Zhang Yongliang ma State Key Joint Laboratory of Environment Simulation and Pollution Control School of Environment State Environmental Protection Key Laboratory of Sources and Control of Air Pollution Complex Beijing Key Laboratory of Indoor Air Quality Evaluation and Control Tsinghua University Beijing 100084 China Ministry of Education Key Laboratory for Earth System Modeling Department of Earth System Science Tsinghua University Beijing 100084 China National Satellite Meteorological Center Beijing 100081 China College of resources and environmental Sciences China Agricultural University Beijing 100094 China

With rapid economic growth and urbanization, the Yangtze River Delta(YRD) region in China has experienced serious air pollution challenges. In this study, we analyzed the air pollution characteristics and their relationship with emissions and meteorology in the YRD region during 2.14.2.16. In recent years, the concentrations of all air pollutants, except O3,decreased. Spatially, the PM2.5, PM10, SO2. and CO concentrations were higher in the northern YRD region, and NO2.and O3 were higher in the central YRD region. Based on the number of non-attainment days(i.e., days with air quality index greater than 100), PM2.5 was the largest contributor to air pollution in the YRD region, followed by O3, PM10, and ***, particulate {2.tter pollution has declined gradually, while O3 pollution *** conditions {2.inly influenced day-to-day variations in pollutant concentrations. PM2.5 concentration was inversely related to wind speed, while O3 concentration was positively correlated with temperature and negatively correlated with relative *** air quality improvement in recent years was {2.inly attributed to emission *** 2.14.2.16, PM2.5, PM10, SO2. NOx, CO, NH3, and volatile organic compound(VOC)emissions in the YRD region were reduced by 2..3%, 2..2., 32.4., 8.1%, 15.9%, 4.5%, and0.3%, respectively. Regional transport also contributed to the air pollution. During regional haze periods, pollutants from North China and East China aggravated the pollution in the YRD region. Our findings suggest that emission reduction and regional joint prevention and control helped to improve the air quality in the YRD region.

关键词： Air pollution Spatiotemporal variation Meteorological condition Emission inventory Regional transport Yangtze River Delta

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An organic visible-photocatalytic-adsorbence mechanism to high-efficient removal of heavy metal antimony ions

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materials Reports(Energy) 2024年第3期4卷 49-59页

作者： Linji Yang Ke Sun tao Liu Ciyuan Huang Libin Zhang Yang Zhou Kai Chen Shangfei Yao Ziyang Zhang Chenfu Zhao Hongxiang Zhu Bingsuo Zou Shuangfei Wang Dongfeng Xue school of Chemistry and Chemical Engineering Guangxi Key Laboratory of Processing for Non-ferrous Metals and Featured MaterialsState Key Laboratory of Featured Metal Materials and Life-cycle Safety for Composite StructuresSchool of ResourcesEnvironment and MaterialsGuangxi UniversityNanning 530004China Department of Biochemistry and Cell Biology YouJiang Medical University for NationalitiesBaise CityGuangxi Zhuang Autonomous Region 533000China Shenzhen Institute for Advanced Study University of Electronic Science and Technology of ChinaShenzhen 518110China

Purification of emerging heavy metal antimony contaminated water based on advanced ingenious *** activated modified coconut shell charcoal(CSC)was synthesized and evaluated as a substrate-supported loaded organic photovoltaic material,PM6:PYIT:PM6-b-PYIT,to prepare a surprisingly highly efficient,stable,environmentally friendly,and recyclable organic photocatalyst(CSC–N–P.P.P),which showed excellent effects on the simultaneous removal of Sb(Ⅲ)and Sb(Ⅴ).The removal efficiency of CSC-N-P.P.P on Sb(Ⅲ)and Sb(Ⅴ)reached an amazing 99.9%in quite a short duration of 15 *** the same time,under ppb level and indoor visible light(~1 W m^(2.),it can be treated to meet the drinking water standards set by the European Union and the *** environmental Protection Agency in 5 min,and even after 2. cycles of recycling,the efficiency is still maintained at about 80%,in addition to the removal of As(Ⅲ),Cd(Ⅱ),Cr(Ⅵ),and Pb(Ⅱ)can also be *** catalyst not only solves the problems of low reuse rate,difficult structure adjustment and high energy consumption of traditional photocatalysts but also has strong applicability and practical *** pioneering approach provides a much-needed solution strategy for removing highly toxic heavy metal antimony pollution from the environment.

关键词： Organic photocatalyst Photocatalysis Purification Indoor visible light

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