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基于SIRT1/PGC-1α信号通路探讨抑眩宁颗粒干预缺血性眩晕的作用机制

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中西医结合心脑血管病杂志 2024年第10期22卷 1787-1792页

作者：王福荣李明坤刘志广马金波开滦总医院赵各庄医院河北唐山063000 开滦总医院河北唐山063000

目的:探讨抑眩宁颗粒对沉默信息调节因子1(SIRT1)/过氧化物酶体增殖物激活受体γ辅激活因子1α(PGC-1α)信号通路的调控作用及对缺血性眩晕大鼠眩晕症状的改善作用。方法:对清洁级SD大鼠进行电刺激逃避反射训练3 d后建立缺血性眩晕模型... 详细信息

目的:探讨抑眩宁颗粒对沉默信息调节因子1(SIRT1)/过氧化物酶体增殖物激活受体γ辅激活因子1α(PGC-1α)信号通路的调控作用及对缺血性眩晕大鼠眩晕症状的改善作用。方法:对清洁级SD大鼠进行电刺激逃避反射训练3 d后建立缺血性眩晕模型。将大鼠分为模型组、抑眩宁颗粒组(6 g/kg)、SIRT1抑制剂(EX527)组(5 mg/kg)、抑眩宁颗粒+EX527组(抑眩宁颗粒6 g/kg+EX5275 mg/kg),各组给予相应药物干预7 d;另取16只清洁级SD大鼠作为假手术组(进行造模前训练,仅穿线不结扎)。采用跳台逃避实验测定跳台逃避潜伏期;多普勒激光血流仪测量大鼠前庭神经核组织血流量,计算血流量下降率;苏木精-伊红染色观察大鼠脑组织病理特征;酶联免疫吸附法检测大鼠脑组织丙二醛(MDA)、超氧化物歧化酶(SOD)活性、一氧化氮(NO)、白细胞介素-1β(IL-1β)、肿瘤坏死因子-α(TNF-α)含量;蛋白免疫印迹法检测大鼠脑组织SIRT1、PGC-1α、B淋巴细胞瘤-2相关X蛋白(Bax)、B淋巴细胞瘤-2(Bcl-2)蛋白表达水平。结果:与假手术组比较,模型组大鼠脑组织神经细胞变形、固缩和凋亡,跳台逃避潜伏期、给药后血流量下降率、脑组织MDA、NO、IL-1β、TNF-α含量、Bax蛋白表达水平升高(P<0.05),SOD活性、SIRT1、PGC-1α和Bcl-2蛋白表达水平降低(P<0.05)。与模型组比较,抑眩宁颗粒组大鼠脑组织凋亡变异的细胞数量减少,胶质周围的正常细胞数量增多,跳台逃避潜伏期、给药后血流量下降率、脑组织MDA、NO、IL-1β、TNF-α含量及Bax蛋白表达水平降低(P<0.05),SOD活性、SIRT1、PGC-1α和Bcl-2蛋白表达水平升高(P<0.05);EX527组大鼠脑组织神经细胞严重变形,固缩和凋亡现象明显,跳台逃避潜伏期、给药后血流量下降率、脑组织MDA、NO、IL-1β、TNF-α含量及Bax蛋白表达水平升高(P<0.05),SOD活性、SIRT1、PGC-1α和Bcl-2蛋白表达水平降低(P<0.05)。EX527可逆转抑眩宁颗粒对缺血性眩晕大鼠的改善作用(P<0.05)。结论:抑眩宁颗粒可能通过激活SIRT1/PGC-1α通路、抑制氧化应激和炎症反应,进而改善缺血性眩晕大鼠眩晕症状。

关键词：缺血性眩晕抑眩宁颗粒沉默信息调节因子1/过氧化物酶体增殖物激活受体γ辅激活因子1α SIRT1/PGC-1α 大鼠实验研究

Ameliorative Effect of Ginsenoside RT-5 on CDDP-Induced Nephrotoxicity

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Wuhan University Journal of Natural Sciences 2015年第4期20卷 343-349页

作者： JIANG Yongtao QIU Xiaolei ma jinbo Lü Guangyao WANG Zongliang ZHANG Jingwen FU Fenghua WANG Hongbo Key Laboratory of Molecular Pharmacology and Drug Evaluation Yantai University Binzhou Medical University State Key Laboratory of Bioactive Substances and Functions of Natural Medicines Institute of Materia MedicaCAMS&PUMC

The aim of this study is to explore whether RT-5, one novel active ginsenosides from Ginseng, has protective effects against cisplatin（CDDP）-induced nephrotoxicity in vivo. One model of acute renal failure induced by CDDP in rats and one model of xenograft tumors of human cervical cancer in nude mice are established. Four groups are assigned： control, CDDP, RT-5, CDDP plus RT-5, in which the RT-5 is administered via oral gavage 24 h before CDDP intraperitoneal injection. The significant increase in blood urea nitrogen and creatinine are induced by CDDP treatment at 6 mg/kg, which are attenuated by pre-treated with RT-5 at 10 mg/kg. RT-5 could ameliorate CDDP-induced morphological damages in kidney by PAS staining, and reduce tubular apoptosis evaluated by TUNEL staining. Pretreatment with RT-5 notably inhibits CDDP-induced oxidative stress in kidney tissues. Interestingly, RT-5 does not interfere with the in vivo anti-cancer effects of CDDP against the growth of xenograft tumors in nude mice. These data suggest that co-treatment RT-5 with CDDP might attenuate the following nephrotoxicity without inhibiting its anti-tumor efficiency, which could provide one novel strategy for cancer treatment in clinics.

关键词： nephrotoxicity ginsenosides RT-5 cisplatin(CDDP)

Rapid growth of a large-scale(600 mm aperture) KDP crystal and its optical quality

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High Power Laser Science and Engineering 2014年第1期2卷 40-44页

作者： Guohui Li Guozong Zheng Yingkun Qi Peixiu Yin En Tang Fei Li Jing Xu Taiming Lei Xiuqin Lin Min Zhang Junye Lu jinbo ma Youping He Yuangen Yao Fujian Institute of Research on the Structure of matter Chinese Academy of Sciences

Potassium dihydrogen phosphate(KDP) single crystals are the only nonlinear crystals currently used for electro-optic switches and frequency converters in inertial confinement fusion research, due to their large dimension and exclusive physical properties. Based on the traditional solution-growth process, large bulk KDP crystals, usually with sizes up to600 × 600 mm2 so as to make a frequency doubler for the facility requirement loading highly flux of power laser, can be grown in standard Holden-type crystallizers, without spontaneous nucleation and visible defects, one to two orders of magnitude faster than by conventional methods. Pure water and KDP raw material with a few ion impurities such as Fe,Cr, and Al(less than 0.1 ppm) were used. The rapid-growth method includes extreme conditions such as temperature range from 60 to 35℃ , overcooling up to 5℃ , growth rates exceeding 10 mm/day, and crystal size up to 600 mm. The optical parameters of KDP crystals were determined. The optical properties of crystals determined indicate that they are of favorable quality for application in the facility.

关键词： large-scale KDP crystals rapid growth optical quality

Heat stress impairs floral meristem termination and fruit development by affecting the BR-SlCRCa cascade in tomato

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Plant Communications 2024年第4期5卷 208-223页

作者： Junqing Wu Pengxue Li Meng Li Danyang Zhu Haochuan ma Huimin Xu Shuang Li jinbo Wei Xinxin Bian Mengyao Wang Yixuan Lai Yuxin Peng Haixiao Li Abidur Rahman Shuang Wu College of Horticulture Haixia Institute of Science and TechnologyFujian Agriculture and Forestry UniversityFuzhou 350002China Department of Plant Bio-Sciences Faculty of AgricultureIwate UniversityMorioka 020-8550Japan United Graduate School of Agricultural Sciences Iwate UniversityMorioka 020-8550Japan These authors contributed equally to this article

Floral meristem termination is a key step leading to carpel initiation and fruit *** frequent occurrence of heat stress due to global warming often disruptsfloral determinacy,resulting in defective fruit ***,the detailed mechanism behind this phenomenon is largely ***,we identify CRABS CLAW a(SlCRCa)as a key regulator offloral meristem termination in *** func-tions as an indispensablefloral meristem terminator by suppressing SlWUS activity through the TOmaTO AGAMOUS 1(TAG1)–KNUCKLES(SlKNU)–INHIBITOR OF MERISTEM ACTIVITY(SlIma)network.A direct binding assay revealed that SlCRCa specifically binds to the promoter and second intron of WUSCHEL(SlWUS).We also demonstrate that SlCRCa expression depends on brassinosteroid homeostasis in theflo-ral meristem,which is repressed by heat stress via the circadian factor EARLY FLOWERING 3(SlELF3).These results provide new insights intofloral meristem termination and the heat stress response inflowers and fruits of tomato and suggest that SlCRCa provides a platform for multiple protein interactions that may epigenetically abrogate stem cell activity at the transition fromfloral meristem to carpel initiation.

关键词： heat stress floral meristem termination BR-CRC fruit development

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对甲砜基甲苯选择性氧化的研究与应用

石油化工高等学校学报 2020年第1期33卷 12-16页

作者：何龙马金波于芳辽宁石油化工大学化学化工与环境学部辽宁抚顺113001 沈阳市环境监测中心站辽宁沈阳110000

对甲砜基甲苯是一种常见的化工原料,经过氧化生成的对甲砜基苯甲醛和对甲砜基苯甲酸是很多医药、兽药、农药的重要中间体。采用氧气为氧化剂,N,N',N″-三羟基异氰尿酸(THICA)为催化剂,Co(OAc)2·4H2O为助剂对甲砜基甲苯的选择性氧化进... 详细信息

对甲砜基甲苯是一种常见的化工原料,经过氧化生成的对甲砜基苯甲醛和对甲砜基苯甲酸是很多医药、兽药、农药的重要中间体。采用氧气为氧化剂,N,N',N″-三羟基异氰尿酸(THICA)为催化剂,Co(OAc)2·4H2O为助剂对甲砜基甲苯的选择性氧化进行考察。当使用乙酸为溶剂时,对甲砜基苯甲酸回收率为96%;使用6 mol/L硝酸为溶剂时,对甲砜基苯甲醛回收率为15%。

关键词：对甲砜基甲苯对甲砜基苯甲酸对甲砜基苯甲醛 THICA 选择性氧化

Two-dimensional-lag complex logistic map with complex parameters and its encryption application

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Chinese Physics B 2024年第5期33卷 324-335页

作者：张芳芳武金波寇磊马凤英吴黎明张雪 School of Information and Automation Engineering Qilu University of Technology (Shandong Academy of Sciences)Jinan 250353China Institute of Oceanographic Instrumentation Qilu University of Technology (Shandong Academy of Sciences)Qingdao 266000China

With the rapid development of internet technology,security protection of information has become more and more prominent,especially information *** the great advantages of chaotic encryption,we propose a 2D-lag complex logistic map with complex parameters(2D-LCLMCP)and corresponding encryption ***,we present the model of the 2D-LCLMCP and analyze its chaotic properties and system stability through fixed points,Lyapunov exponent,bifurcation diagram,phase diagram,***,a block cipher algorithm based on the 2D-LCLMCP is proposed,the plaintext data is preprocessed using a pseudorandom sequence generated by the *** on the generalized Feistel cipher structure,a round function F is constructed using dynamic S-box and DNA encoding rules as the core of the block cipher *** generalized Feistel cipher structure consists of two F functions,four XOR operations,and one permutation operation per *** symmetric dynamic round keys that change with the plaintext are generated by the ***,experimental simulation and performance analysis tests are *** results show that the block cipher algorithm has low complexit,good diffusion and a large key *** the block length is 64 bits,only six rounds of encryption are required to provide sufficient security and robustness against cryptographic attacks.

关键词： logistic map block ciphers chaotic system encryption

4-异丁酰氧基-2-环庚烯-1-酮的合成研究

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石油化工高等学校学报 2023年第2期36卷 78-82页

作者：曾天映马金波于芳辽宁石油化工大学石油化工学院辽宁抚顺113001 沈阳市环境监测中心站辽宁沈阳110000

通过异丁酰氯和4-羟基-2-环庚烯-1-酮缩合制备了4-异丁酰氧基-2-环庚烯-1-酮。在无缩合剂的条件下,考察了4-二甲氨基吡啶(DmaP)的物质的量、反应温度、反应时间等对酯化反应的影响。结果表明,该反应较佳的DmaP的物质的量为0.3 mmol,较... 详细信息

通过异丁酰氯和4-羟基-2-环庚烯-1-酮缩合制备了4-异丁酰氧基-2-环庚烯-1-酮。在无缩合剂的条件下,考察了4-二甲氨基吡啶(DmaP)的物质的量、反应温度、反应时间等对酯化反应的影响。结果表明,该反应较佳的DmaP的物质的量为0.3 mmol,较佳温度为25℃,较佳的反应时间为3 h;在以上反应条件下,酯化产物的产率为89%。核磁表征结果表明,合成的产物为目标产物4-异丁酰氧基-2-环庚烯-1-酮。该反应条件温和,无需缩合剂参与,产率高,符合绿色化学发展的概念。

关键词：酯化反应无缩合剂产率

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气相色谱法测定结球甘蓝中三氟甲吡醚农药的残留

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天津农业科学 2022年第11期28卷 49-54页

作者：马金波山西普华检测技术有限公司山西太原030032

为建立测定结球甘蓝中三氟甲吡醚农药残留量的气相色谱检测方法,采用加压流体萃取-多功能浓缩技术,利用丙酮进行加压流体萃取,萃取液经脱水、浓缩、溶剂交换后用气相色谱-电子捕获检测器(ECD)检测,以外标法定量。该方法检出限(信噪比法... 详细信息

为建立测定结球甘蓝中三氟甲吡醚农药残留量的气相色谱检测方法,采用加压流体萃取-多功能浓缩技术,利用丙酮进行加压流体萃取,萃取液经脱水、浓缩、溶剂交换后用气相色谱-电子捕获检测器(ECD)检测,以外标法定量。该方法检出限(信噪比法)三氟甲吡醚0.004 mg·kg^(-1);0.1~10 mg·L^(-1)浓度范围内线性关系良好,线性相关系数r>0.990;3个浓度添加水平(低、中、高)加标回收率为85.4%~117.0%;利用建立的方法对结球甘蓝样品进行测定,结果相对标准偏差1.5%。该方法提取效率高,样品提取浓缩后测定准确度高,重现性好,检出限低,适用于结球甘蓝中三氟甲吡醚农药残留量的测定。

关键词：三氟甲吡醚残留气相色谱(ECD)

气质联用测定韭菜中苯醚甲环唑、氟虫腈、甲拌磷三种农药的残留

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河南化工 2022年第10期39卷 52-55页

作者：马金波山西普华检测技术有限公司山西太原030032

采用固相萃取净化-多功能氮吹浓缩技术,建立了同时测定韭菜中苯醚甲环唑、氟虫腈、甲拌磷3种农药残留量的气相色谱-质谱联用检测方法。该方法净化效果好,样液损失率低,定性及定量结果准确,重现性性好,检出限低,适用于韭菜中3种农药残留... 详细信息

采用固相萃取净化-多功能氮吹浓缩技术,建立了同时测定韭菜中苯醚甲环唑、氟虫腈、甲拌磷3种农药残留量的气相色谱-质谱联用检测方法。该方法净化效果好,样液损失率低,定性及定量结果准确,重现性性好,检出限低,适用于韭菜中3种农药残留量的同时测定。

关键词：苯醚甲环唑氟虫腈甲拌磷残留量气质联用