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Commensal <i>E. Coli</i>Strains Uniquely Alter the ECm Topography Independent of Colonic Epithelial Cells

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Journal of Biomaterials and Nanobiotechnology 2012年第1期3卷 70-78页

作者： Shruthi S. Bharadwaj Victor Nekrasov ramana Vishnubhotla Crystal Foster Sarah C. Glover Department of Bioengineering University of Illinois at Chicago Chicago USA Department of medicine University of Florida Gainesville USA

The relationship between commensal bacteria and the epithelial cells lining the colon is normally symbiotic. However, in the setting of diseases which lead to a loss of the protective mucosal layer such as inflammatory bowel disease or colon cancer, commensal bacteria gain the ability to alter both the behavior of epithelial cells as well as their surrounding extra cellular matrix (ECm). While much work has been done to understand the effects of bacteria on diseased epithelial cells in the colon, very little has been done to understand their impact on the ECm. In our previous work, we have shown that topographical changes in the ECm on the luminal side of the colon have a profound influence on the behavior of colonic epithelial cells. However, we do not understand all of the mechanisms that lead to changes in the ECm topography. This study aimed to understand the role that commensal E. coli strains play in altering the ECm topography of type-1 collagen scaffolds. To do this, 1.2 mg/ml type 1 collagen scaffolds were infected with various commensal bacterial strains. At 24 hours post-infection collagen fiber dimensions and substrate topography were determined using standard molecular biology techniques and advanced imaging. Intriguingly, all of the commensal E. coli strains showed some form of substrate degradation. Especially in the case of commensal E. coli strain HS4, maximum nano-scaled protrusions were observed. This data suggests, for the first time, that studying the effects of bacteria alone on the ECm may be critical to improving our understanding of how the cellular microenvironment changes in both health and disease.

关键词： Topography Extra Cellular matrix Commensal Bacteria Western Blot Collagen Fiber

molecular Structure, Vibrational Analysis and First Order Hyperpolarizability of 4-methyl-3-Nitrobenzoic Acid Using Density Functional Theory

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Optics and Photonics Journal 2015年第3期5卷 91-107页

作者： Jyothi Prashanth Gaddam Ramesh Jarupula Laxman Naik Jai Kishan Ojha Byru Venkatram Reddy Gandham ramana Rao Department of Physics Kakatiya University Warangal India Department of Physics Govt. Degree & P.G. College Adilabad India

The Fourier Transform Infrared (FTIR) and FT-Raman spectra of 4-methyl-3-nitrobenzoic acid have been recorded in the range 4000 - 400 cm-1 and 3500 - 50 cm-1, respectively. The optimized geometry of the molecule, its vibrational frequencies along with corresponding intensities have been computed using the Density Functional Theory (DFT) employing B3LYP/6-311++G basis set. The scaled values of harmonic vibrational frequencies obtained in the computations have been compared with their experimental counter parts. The scaling factors have been refined to reproduce the frequencies with an RmS error of 11.68 cm-1 between the experimental and computed frequencies. The theoretically constructed spectra agree satisfactorily with those of experimental spectra. First order hyperpolarizability constants have also been evaluated.

关键词： 4-methyl-3-Nitrobenzoic Acid Vibrational Spectra DFT First Order Hyperpolarizability

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DVD刻录机的自适应写策略方法

记录媒体技术 2007年第3期 43-48页

作者： Vangala V.ramana 宋洁飞利浦电子

一、引言目前市场上存在着大量的光盘仅可以勉强达到光存储的技术规范要求,有些光盘甚至在某些方面完全不能达到要求,不过由于其价格低廉,仍然有着巨大的消费市场。这类光盘上存在的不可靠介质信息、生产过程中染料的不良操作以及不合... 详细信息

一、引言目前市场上存在着大量的光盘仅可以勉强达到光存储的技术规范要求,有些光盘甚至在某些方面完全不能达到要求,不过由于其价格低廉,仍然有着巨大的消费市场。这类光盘上存在的不可靠介质信息、生产过程中染料的不良操作以及不合适的介质设计等都会导致光盘的刻录质量下降,其中有些盘片上没有记录生产厂家资料和介质信息。

关键词：刻录机相变光盘盘片倍速刻录速度信息符策略方法生产过程技术规范要求光学头

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management of proximal tibial stress fracture associated with advanced knee osteoarthritis:A systematic review

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Chinese Journal of Traumatology 2024年第3期27卷 147-152页

作者： mukesh Kumar Saini mahendra Singh Devendra Singh Prem manohar Seervi Pera Jayavardhan Reddy Neelam ramana Reddy Department of Orthopaedics Dr SN Medical CollegeJodhpur342011India Department of Orthopaedics Star HospitalsB Block Banjara HillsHyderabad500034India

Purpose:Tibial stress fracture associated with knee osteoarthritis is an unusual and difficult clinical *** is no clear existing treatment guideline for this uncommon clinical *** aim of this study is to review the impact of various treatment options for patients with advanced knee osteoarthritis associated with proximal tibial stress ***:The study was performed using the databases of Pubmed and *** index for non-randomized studies score was used to evaluate the included studies’*** concluded data included the treatment approach,reported outcome measure,and time to fracture *** literature search was started in December 2021 and accomplished in January 2022.A narrative description of the different methods and comparison of their results were ***:Out of total assessed 69 studies,9 studies were included in our *** commonest treatment approach used was total knee arthroplasty by long tibial stem *** mean preoperative knee society score and knee functional score were 30.62 and 23.17,*** mean postoperative knee society knee score was 86.87,while the functional score was *** average reported time to achieve fracture union was 4 months(a range of 2.07-5.50 months).Conclusion:The optimal clinical outcome for treating either acute or mobile tibial stress fracture in patients with advanced knee osteoarthritis can be achieved with long stem total knee ***,due to heterogeneity of data,comparison of different treatment options for chronic proximal tibial stress fracture mal-union/non-union coexisting with knee osteoarthritic and such inferences need to be judged cautiously.

关键词： Tibial stress fracture Knee osteoarthritis Total knee arthroplasty

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金属、矿物废料加工及其二次回收

矿产保护与利用 1988年第2期 50-55页

作者： ramana G.Reddy 黄永校

绪言美国每年产生的贵金属化学废料大约有四千万吨。为了回收其中所含的贵金属和极需的金属,这些废料中估计有5%正在得到再利用或回收。这些化学废料的剩余部分可用焚化、倒入海中和堆放在陆地上的方法进行处理。本文试图对1985～1986... 详细信息

绪言美国每年产生的贵金属化学废料大约有四千万吨。为了回收其中所含的贵金属和极需的金属,这些废料中估计有5%正在得到再利用或回收。这些化学废料的剩余部分可用焚化、倒入海中和堆放在陆地上的方法进行处理。本文试图对1985～1986年间有关金属、矿物废料的加工,二次回收和安全处置方面所公布的研究工作作一评述。

关键词：金属金属材料金属废料废料处理回收工艺矿物

Characterization of Biofilms and mineralogical Scale in Underground Injection Well Disposal of Landfill Leachate and Industrial Wastewater Streams

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Journal of Geoscience and Environment Protection 2019年第11期7卷 69-91页

作者： Daniel E. meeroff Bishow Shaha Frederick Bloetscher Nwadiuto Esiobu Brian mercer Donald mcCorquordale ramana Kari michael Bennett Department of Civil Environmental & Geomatics Engineering Florida Atlantic University Boca Raton FL USA Department of Biological Sciences Florida Atlantic University Sanson Life Sciences Boca Raton FL USA Applied Biotech Inc. Plantation FL USA Solid Waste Authority of Palm Beach County West Palm Beach FL USA AECOm/URS West Palm Beach FL USA

Deep-well injection is a cost-effective alternative for industrial wastewater disposal, given the appropriate geology. Fouling of injection well tubing by biofilm or scale is common and reduces the effective diameter of the pipe, which results in increased wellhead pressure and lower injectivity. A detailed microbiological composition of biofilms and abiotic fingerprints use of mineral scale from an injection well has not been reported before. Therefore, biofilm and mineral scale samples from three depth intervals within a deep injection well (surface zone, D1= 0 - 61 m;intermediate zone, D2 = 62 - 457 m;and above the injection zone, D3 = 458 - 884 m) with recurrent biofilm development were collected to characterize the mineral composition and microbial community DNA. X-ray diffraction (XRD) analysis of the solids confirms the composition of the solids collected was mostly calcium carbonate (CaCO3) with minor contributions from common mineral salts. microbiological fingerprinting suggests that methanogenic archaea and sulfate-reducing bacteria both of which are anaerobic biofilm producers were the most prevalent members of the prokaryotic community at all sampled depths. methanosarcinae spp. increased with increasing depths, unlike other archaea. A non-pathogenic biofilm-producing Entamoeba dispar was the most prevalent member of the microbial domain (>30%) in all samples but was highest at the middle depth. The Chao alpha diversity indices for bacteria, viruses, and protozoans were highest at the shallow depth and gradually declined with increasing depth. The prevalent species above the injection zone depth are not barophilic organisms that thrive at high pressures, rather they are sulfate-reducing bacteria involved in anaerobic dissimilatory sulfate metabolism.

关键词： Injection Well Leachate Industrial Wastewater Disposal Biofilm microbiology Well Rehabilitation metagenomic Sequencing

STAT1 and STAT2 Null Cells Are Resistant to RNA-Induced Apoptosis Due to Deficiency in Constitutive and Inducible Apoptosis-Regulating Genes

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American Journal of molecular Biology 2020年第3期10卷 165-187页

作者： Farag Bleiblo Paul michael Chilakamarti V. ramana T. C. Tai Joseph E. Parrillo Anand Kumar Aseem Kumar Department of microbiology Faculty of Science University of Benghazi Benghazi Libya Department of Chemistry and Biochemistry and the Biomolecular Sciences Programme Laurentian University Sudbury Canada Department of medicine Dartmouth-Hitchcock Medical Center Dartmouth Medical School Lebanon NH USA Division of Cardiovascular Disease and Critical Care medicine Cooper University Hospital Robert Wood Johnson Medical School Camden NJ USA Section of Critical Care medicine University of Manitoba Winnipeg Canada

Although much progress has been made in identifying the signaling pathways that mediate viral RNA-induced apoptosis and activation of interferon-stimulated genes, the role that bacterial RNA plays in regulating these responses has remained undetermined. Herein, we identified bacterial RNA as a novel inducer of the apoptotic cell death. Unlike the parental cells, STAT1 and STAT2 mutants display apoptotic defects which were reversed by restoring the expression of wild type proteins. While STAT1 mutants lacking tyrosine-701 or a functional SH2 domain were effective as the wild-type protein in restoring the apoptotic response, the mutant carrying a point mutation at serine-727 of STAT1 was resistant to bacterial RNA-induced apoptosis. We also determined that the lack of apoptosis in the STAT1 and STAT2 mutants was correlated with the constitutive and inducible activation of apoptosis regulating proteins. Furthermore, we show that bacterial RNA induces transcriptional activation of STAT1, STAT2, IRF1, and ISGF3, which was impaired in STAT1 or STAT2 mutants. These observations suggested that the participation of STATs in regulating the apoptotic response is independent of their downstream functions as cytokine-induced transcriptional activators. In addition to bacterial immunity, the results presented here may also have implications in cellular pathophysiology and RNA-based therapy.

关键词： STAT1 STAT2 Apoptosis Bacterial RNA

Co-Integration models for Koyna and Warna Reservoirs, India

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International Journal of Geosciences 2015年第10期6卷 1173-1178页

作者： D. V. ramana J. Pavan Kumar R. N. Singh R. K. Chadha National Geophysical Research Institute (CSIR) Hyderabad India Indian National Centre for Ocean Information Services (moES) Hyderabad India

Koyna region, a seismically active region, has many time series observations such as seismicity, reservoir water levels, and many bore well water levels. One of these series is used to predict others since these parameters are interlinked. If these series were stationary, we used correlation analysis. However, it is seen that maximum of these time series are nonstationary. In this case, co-integration method is used that is extracted from econometrics and forecast is possible. We have applied this methodology to study time series of reservoir water levels of this region and we find them to be co-integrated. Therefore, forecast of water levels for one of the reservoir is done from the other as these will never drift apart too much. The outcomes demonstrate that a joint modelling of both data sets based on underlying physics resolves to be sparingly useful for understanding predictability issues in reservoir induced seismicity.

关键词： Co-Integration Seismicity Forecast Koyna-Warna Reservoirs Water Levels

锶掺杂的La_(2)Ce_(2)O_(7)烧绿石催化甲烷氧化偶联性能研究

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现代化工 2022年第4期42卷 109-115,122页

作者：武国号 Palle ramana murthy 陈冰冰张晓石川宋春山郭新闻大连理工大学化工学院精细化工国家重点实验室辽宁大连116024 香港中文大学理学院化学系中国香港999077

基于烧绿石类材料热稳定性高、晶格氧流动性可调控的特点,通过溶胶-凝胶法制备了La_(2)Ce_(2)O_(7)催化剂,向其中掺杂碱土金属Sr,研究了其在甲烷氧化偶联(OCm)反应中的催化性能。通过一系列表征确认了La_(2)Ce_(2)O_(7)样品的缺陷立方... 详细信息

基于烧绿石类材料热稳定性高、晶格氧流动性可调控的特点,通过溶胶-凝胶法制备了La_(2)Ce_(2)O_(7)催化剂,向其中掺杂碱土金属Sr,研究了其在甲烷氧化偶联(OCm)反应中的催化性能。通过一系列表征确认了La_(2)Ce_(2)O_(7)样品的缺陷立方萤石结构;锶掺杂不仅显著提高了晶格氧的活动性,还提升了催化剂表面强碱位点的比例;与OCm反应活性关联发现,晶格氧流动性增强以及表面强碱位点的增加是提高OCm反应活性的关键因素;在优化的La_(1.5)Sr_(0.5)Ce_(2)O_(7)催化剂上,800℃下可以获得57%的C_(2)选择性和14%以上的C_(2)收率,连续反应30 h性能保持稳定。

关键词： La_(2)Ce_(2)O_(7) 锶掺杂晶格氧物种甲烷氧化偶联

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