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Extrahepatic cholestasis associated with paracoccidioidomycosis:Challenges in the differential diagnosis of biliopancreatic neoplasia

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World Journal of Gastrointestinal Oncology 2024年第6期16卷 2531-2540页

作者： Jose Sebastiao dos Santos Vitor de moura Arrais William Jose Rosseto ferreira Ricardo Ribeiro Correa filho mariangela Ottoboni Brunaldi Rafael Kemp Ajith Kumar Sankanrakutty Jorge Elias Junior Fernando Bellissimo-Rodrigues Roberto martinez Edson Zangiacomi martinez Jose Celso Ardengh Faculdade de medicina de Ribeirao Preto da Universidade de Sao Paulo(FmRP-USP) Departamento de Cirurgia e AnatomiaRibeirao Preto 14049-900Sao PauloBrazil Universidade de Sao Paulo Faculdade de Medicina de Ribeirao Preto da Universidade de Sao Paulo(FMRP-USP)Departamento de PatologiaRibeirao Preto 14048900Sao PauloBrazil Universidade de Sao Paulo Faculdade de Medicina de Ribeirao Preto da Universidade de Sao Paulo(FMRP-USP)Departamento de Imagens MédicasHematologia e Oncologia ClínicaRibeirao Preto 14048900Sao PauloBrazil Universidade de Sao Paulo Faculdade de Medicina de Ribeirao Preto da Universidade de Sao Paulo(FMRP-USP)Departamento de Medicina SocialRibeirao Preto 14015-010Sao PauloBrazil Universidade de Sao Paulo Faculdade de Medicina de Ribeirao Preto da Universidade de Sao Paulo(FMRP-USP)Departamento de Clínica MédicaRibeirao Preto 14015-010Sao PauloBrazil Universidade de Sao Paulo Faculdade de Medicina de Ribeirao Preto da Universidade de Sao Paulo(FMRP-USP)Departamento de Cirurgia e AnatomiaRibeirao Preto 14049-900Sao PauloBrazil and Hospital MoriahServico de Endoscopia DigestivaSao Paulo 04084-002Sao PauloBrazil

BACKGROUND Paracoccidioidomycosis(PCm)may involve the hepatic pedicle and peripan creatic lymph nodes,cause damage to the bile duct and manifest,exceptionally,in combination with extrahepatic cholestasis(EHC),making investigation and treatment *** To investigate the management of patients with visceral PCm admitted with *** All patients diagnosed with PCm treated in a public,tertiary teaching hospital between 1982 and 2020 were retrospectively *** also identified with EHC were allocated to two groups according to the treatment approach for the purpose of comparing clinical,laboratory,and imaging findings,resources used for etiological diagnosis,treatment results,and *** analyses were performed using the linear mixed-effects model(random and fixed effects),which was adjusted using the PROC mIXED procedure of the SAS®9.0 software,and Fisher’s exact *** Of 1645 patients diagnosed with PCm,40(2.4%)had *** these,20(50.0%)lived in the rural area and 29(72.5%)were men,with a mean age of 27.1 years(3-65 years).Jaundice as first symptom and weight loss of at least 10 kg were observed in 16 patients(40.0%),and a mass in the head of the pancreas was observed in 8(20.0%).The etiological diagnosis was made by tissue collection during surgery in 4 cases(10.0%)and by endoscopic methods in 3 cases(7.5%).Twenty-seven patients(67.5%)received drug treatment alone(Group 1),whereas 13(32.5%)underwent endoscopic and/or surgical procedures in combination with drug treatment(Group 2).EHC was significantly reduced in both groups(40.7% in Group 1,with a mean time of 3 months;and 38.4% in Group 2,with a mean time of 7.5 months),with no statistically significant difference between *** recurrence rates,associated mainly with treatment nonadherence,were similar in both groups:37% in Group 1 and 15.4% in Group *** mortality rate was 18.5% in Group 1 and 23% in Group 2,with survival estimates of 71.3% and 72.5%,respectively,with

关键词： Cholestasis Jaundice Obstructive Blastomycosis Paracoccidioides Diagnosis Treatment

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R&D of back-end electronics for improved resistive plate chambers for the phase 2 upgrade of the CmS end-capmuon system

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Radiation Detection Technology and methods 2022年第3期6卷 306-316页

作者： H.Kou Z.-A.Liu J.Zhao J.Song Q.Hou W.Diao P.Cao W.Gong N.Wang A.Samalan m.Tytgat m.El Sawy G.A.Alves F.marujo E.A.Coelho F.Torres Da Silva De Araujo E.m.Da Costa H.Nogima A.Santoro S.Fonseca De Souza D.De Jesus Damiao m.Thiel m.barroso ferreira filho K.mota Amarilo A.Aleksandrov R.Hadjiiska P.Iaydjiev m.Rodozov m.Shopova G.Sultanov A.Dimitrov L.Litov B.Pavlov P.Petkov A.Petrov E.Shumka S.J.Qian C.Avila D.Barbosa A.Cabrera A.Florez J.Fraga J.Reyes Y.Assran m.A.mahmoud Y.mohammed I.Laktineh G.Grenier m.Gouzevitch L.mirabito K.Shchablo C.Combaret W.Tromeur G.Galbit A.Luciol X.Chen I.Bagaturia I.Lomidze Z.Tsamalaidze V.Amoozegar B.Boghrati m.Ebraimi E.Zareian m.mohammadi Najafabadi m.Abbrescia G.Iaselli G.Pugliese F.Loddo N.De Filippis R.Aly D.Ramos W.Elmetenawee S.Leszki I.margjeka D.Paesani L.Benussi S.Bianco D.Piccolo S.meola S.Buontempo F.Carnevali L.Lista P.Paolucci F.Fienga A.Braghieri P.Salvini P.montagna C.Riccardi P.Vitulo E.Asilar J.Choi T.J.Kim S.Y.Choi B.Hong K.S.Lee H.Y.Oh J.Goh I.Yu C.Uribe Estrada I.Pedraza H.Castilla-Valdez R.L.Fernandez A.Sanchez-Hernandez E.Vazquez m.Ramirez-Garcia N.Zaganidis A.Radi H.Hoorani S.muhammad A.Ahmad I.Asghar m.A.Shah W.A.Khan J.Eysermans I.Crotty on behalf of the CmS muon Group Department of Physics and Astronomy Ghent UniversityProeftuinstraat 869000 GhentBelgium UniversitéLibre de Bruxelles Avenue Franklin Roosevelt50-1050 BruxellesBelgium Centro Brasileiro Pesquisas Fisicas R.Dr.Xavier Sigaud150UrcaRio de JaneiroRio de Janeiro 22290-180Brazil Department de Fisica Nuclear e Altas Energias Instituto de FisicaUniversidade do Estado do Rio de JaneiroRua Sao Francisco Xavier524Rio de JaneiroRio de Janeiro 20559-900Brazil Bulgarian Academy of Sciences Institute for Nuclear Researches and Nuclear EnergyTzarigradsko Shaussee Boulevard 721784 SofiaBulgaria Faculty of Physics University of Sofia5 James Bourchier Boulevard1164 SofiaBulgaria School of Physics Peking UniversityBeijing 100871China State Key Laboratory of Particle Detection and Electronics Institute of High Energy PhysicsChinese Academy of SciencesBeijing 100049China University of Chinese Academy of Sciences B325Main BuildingNo.19(A)Yuquan RoadShijingshan DistrictBeijing 100049China Universidad de Los Andes Carrera 1No.18A-12BogotáColombia Egyptian Network for High Energy Physics Academy of Scientific Research and Technology101 Kasr El-Einy St.CairoEgypt Suez University SuezCairo RoadElsalam CitySuez 43522Egypt Center for High Energy Physics(CHEP-FU) Faculty of ScienceFayoum UniversityEl-Fayoum 63514Egypt Univ Lyon Univ Claude Bernard Lyon 1CNRS/IN2P3IP2I LyonUMR 582269622 VilleurbanneFrance Georgian Technical University 77 Kostava Str.0175 TbilisiGeorgia School of Particles and Accelerators Institute for Research in Fundamental Sciences(IPM)P.O.Box 19395-5531 TehranIran School of Engineering Damghan UniversityDamghan ***Iran INFN Sezione di BariVia Orabona 470126 BariItaly INFN Laboratori Nazionali di Frascati(LNF)Via Enrico Fermi 4000044 FrascatiItaly INFN Sezione di NapoliComplesso University Monte S.AngeloVia Cintia80126 NapoliItaly Dipartimento di Ingegneria Elettrica e delle Tecnologie dell’Informazione UniversitàDegli Studi di Napoli Federico II80126 NapoliI

Purpose The Large Hadron Collider(LHC)at European Organization for Nuclear Research is planned to be upgraded to the high luminosity *** the luminosity makes muon triggering reliable and ofﬂine reconstruction very *** enhance the redundancy of the Compact muon Solenoid(CmS)muon system and resolve the ambiguity of track reconstruction in the forward region,an improved Resistive Plate Chamber(iRPC)with excellent time resolution will be installed in the Phase-2 CmS *** iRPC will be equipped with Front-End Electronics(FEE),which can perform high-precision time measurements of signals from both ends of the *** Back-End Electronics(BEE)need to be researched and developed to provide sophisticated functionalities such as interacting with FEE with shared links for fast,slow control(SC)and data,in addition to trigger primitives(TPs)generation and data acquisition(DAQ).method The BEE prototype uses a homemade hardware board compatible with the mTCA standard,the back-end board(BEB).BEE interacts with FEE via a bidirectional 4.8 Gbps optical paired-link that integrates clock,data,and control *** clock and fast/slow control commands are distributed from BEB to the FEE via the *** uplink is used for BEB to receive the time information of the iRPC’sﬁred strips and the responses to the fast/slow control *** have a pipelined detector data for clusterﬁnding operation,recover(Demux)the time relationship of which is changed due to the transmission protocol for the continuous incoming mUXed data from *** at each bunch crossing(BX),clusteringﬁred strips that satisfy time and spatial constraints to generate *** incoming raw mUXed detector data and TPs in a time window and latency based on the trigger signal are read out to the DAQ *** Ethernet(GbE)of SiTCP and commercial 10-GbE are used as link standards for SC and DAQ,respectively,for the BEB to interact with the *** The joint test results of

关键词： CmS iRPC BEE TP DAQ SC

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Evaluation of Intraobserver and Interobserver Reliability of mechanical Axis Alignment measure of the Lower Limb through the Panoramic Radiograph in Patients in the Preoperative and Postoperative Periods of Total Knee Arthroplasty

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Open Journal of Orthopedics 2020年第9期10卷 221-233页

作者： murilo barroso matos José Leonardo Rocha de Faria Douglas mello Pavão márcio Liu Sandt Breno Chaves de Almeida Pigozzo Pedro Guilme Teixeira de Sousa filho Rodrigo Pires e Albuquerque CLIFOR-Orthopedics and Fractures Clinic Vitória Brazil Knee Surgery Center National Institute of Traumatology and Orthopedics Rio de Janeiro Brazil Genutarso-Orthopedics Clinic Rio de Janeiro Brazil Genu Surgery Group Fortaleza Brazil

Objective: To evaluate intraobserver and interobserver reliability of the measurement of the mechanical axis of the lower limb by means of the panoramic radiograph of lower limbs by physicians with different levels of medical practice. Method: A retrospective cross-sectional study with preoperative and postoperative radiographic analysis of a group of 100 patients submitted to total knee arthroplasty. Results: It was observed that there is a highly significant intraobserver and interobserver agreement (p Conclusion:mily:Verdana;"> The panoramic radiograph of the lower limbs is a reliable method for measuring the mechanical axis of the lower limb in patients submitted to total knee arthroplasty, regardless of the level of medical practice of the evaluator.

关键词： Knee mechanical Axis Total Knee Arthroplasty

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Impact of cytomegalovirus reactivation just before liver transplantation:A prospective cohort study

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World Journal of Gastrointestinal Pathophysiology 2021年第3期12卷 51-58页

作者： Claudio marcel B Stadnik Cassia ferreira B Caurio Edison m Rodrigues-filho Wagner L Nedel Guido PC Cantisani maria L Zanotelli Alessandro C Pasqualotto Infection Control Department Santa Casa de Misericordia de Porto AlegrePorto Alegre 90075075RSBrazil molecular Biology Laboratory Santa Casa de Misericordia de Porto AlegrePorto Alegre 90075075RSBrazil Intensive Care Santa Casa de Misericordia de Porto AlegrePorto Alegre 90075075RSBrazil Liver Transplant Unit Santa Casa de Misericordia de Porto AlegrePorto Alegre 90075075RSBrazil Infectious Diseases Unit Universidade Federal de Ciencias da Saude de Porto AlegrePorto Alegre 90035075RSBrazil

BACKGROUND Cytomegalovirus(CmV)is the most common viral pathogen after liver transplantation(LT).Although reactivation of CmV infection is generally described in the context of immunosuppression,it has also been described in critically ill immunocompetent patients including cirrhotic *** To determine the incidence of reactivated CmV prior to *** This was a prospective cohort study evaluating adult patients who underwent LT between 2014 and 2016.A plasma sample was obtained from all patients for CmV quantitative real-time PCR testing right before *** were followed for at least 1 year to assess the following outcomes:Incidence of CmV infection,organ rejection and overall *** A total of 72 patients were *** patients died before transplantation,thus 68 patients were followed up for a median of 44 mo(20-50 mo).In 23/72 patients(31.9%)CmV was reactivated before ***-transplantation,16/68(23.5%)patients had CmV infection and that was significantly associated with the recipient being CmV negative and a CmV-positive *** CmV reactivation was not associated with overall mortality(log rank:0.9).CONCLUSION This study shows that CmV infection is common in patients with chronic liver disease just before LT,but the clinical impact of this infection seems to be negligible.

关键词： Liver transplantation Cytomegalovirus infection Quantitative real-time PCR Risk factors Liver cirrhosis molecular biology

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Dynamical Donwscaling for Railroad Areas in Eastern Amazon and Southeastern Brazil:Current Climate and Near-Future Projections

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Atmospheric and Climate Sciences 2014年第2期4卷 155-163页

作者： Everaldo B.De Souza Bergson C.de moraes Douglas B.S.ferreira Luiz Gylvan meira filho Instituto Tecnológico VALE—ITV Grupo de Pesquisa sobre Mudancas do ClimaBelémBrazil

We performed a dynamic downscaling using REGCm4 regional model driven by mPI global model for current (1990/2012) and near-future (2015/2039) climate in order to characterize the seasonal rainfall regimes throughout the railroad areas in eastern Amazon and southeastern Brazil. The analysis of observational data for the current climate indicated the existence of pronounced spatial variations in rainfall regime across railroad regions during both the rainy and dry seasons. Although models have presented generalized underestimation, the regional model showed improvements on spatial representation and intensity of the rainfall in comparison with global model results. We reported the future projections taking into account the correction of simulated rainfall by the values of the biases found in each respective seasonal regime, so that the results are expressed by percentage changes of the future (2015/2037) relative to the current climate patterns. For the railroad in eastern Amazon, projections indicate a weak decrease of rainfall of about -15% in the rainy season (January to may), however during the dry season (June to October) are expected drastic reductions between -70% and -90% in south (Carajás in Pará state) and north (Sao Luis in maranhao state) portions. Conversely, for the railroad in southeast Brazil, model projections point out for an increased rainfall regime during the rainy season (October to February) around +30% to +40% in the east part of the region over the Espírito Santo state.

关键词： Regional Climate modeling Rainy and Dry Seasons Climate Change Brazil Climate REGCm4

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Comparative Skill of Numerical Weather Forecasts in Eastern Amazonia

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Atmospheric and Climate Sciences 2013年第3期3卷 355-363页

作者： Bergson Cavalcanti de moraes Douglas Batista da Silva ferreira Luiz Gylvan meira filho Juarez Ventura de Oliveira Everaldo Barreiros de Souza Pedro Pereira ferreira Junior Renata Kelen Cardoso Camara Edson Jose Pda Rocha Joao Batista m.Ribeiro Vale Institute of Technology BelemBrazil Federal University of Para(UFPa) BelemBrazil

The present study evaluates the performance of three numerical weather forecasting models: Global Forecast System (GFS), Brazilian Regional Atmospheric modelling System (BRAmS) and ETA Regional model (ETA), by means of the mean Error (mE) and the Root mean Square Error (RmSE), during the most rainy four months period (January to April 2012) on Eastern Amazonia. The models displayed errors of superestimation and underestimation with respect to the observed precipitation, mainly over center-north of Pará and all of Amapá, where the precipitation is higher. Among the analyzed models, GFS shows the best performance, except during January and march, when the model to underestimated precipitation, possibly due to the anomalously high values recorded.

关键词： meteorological models mean Error Root mean Square Error Precipitation Eastern Amazonia

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Traditional Chinese medicine wrist pulse-taking is associated with pulse waveform analysis and hemodynamics in hypertension

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Journal of Integrative medicine 2016年第2期14卷 100-113页

作者： Nathalia Gomes Ribeiro de moura Ivan Cordovil Arthur de Sa ferreira Postgraduate Program of Rehabilitation Science Augusto Motta University Center21041-010Rio de JaneiroR JBrazil Division of Arterial Hypertension National Institute of Cardiology22240-002Rio de JaneiroR JBrazil Department of Physical Therapy Salgado de Oliveira University24030-060NiteroiR JBrazil

BACKGROUND： Pulse wave analysis（PWA） quantifies the phenomenon of pulse waveform propagation in patients with cardiovascular diseases, whereas pulse image analysis（PIA） is a subjective examination in traditional Chinese medicine. OBJECTIVE： This study evaluated the association of PIA with PWA and hemodynamics in patients with hypertension. DESIGN, SETTING, PARTICIPANTS AND INTERVENTIONS： This observational, cross-sectional study enrolled 45 patients（26 men,（55.2± 10.3） years, systolic blood pressure（155± 28） mm Hg, diastolic blood pressure（93± 17） mm Hg） for assessment of clinical and laboratorial data. mAIN OUTCOmE mEASURES： Primary outcomes comprised： pattern differentiation based on an automated method; PIA at the radial artery using the ‘simultaneous pressing＇ method for identification of factors such as strength（strong/weak）, depth（superficial/deep）, and speed（fast/moderate/slow）; and PWA at the same artery using a noninvasive system. RESULTS： Significant multivariate main effects were observed for depth（l=0.648, F_（5,29）=3.149, P=0.022, h^2 =0.352）, strength（l=0.608, F_（5,29） =3.736, P=0.010, h^2 =0.392）, and speed（l=0.535, F_（5,29） =5.302, P=0.002, h^2 =0.465）. General effects comprised high values of PWA and blood pressure for superficial, strong, and fast pulse images. A strong pulse was found for pulse pressure ≥ 62.5 mm Hg and systolic blood pressure ≥ 149.5 mm Hg, whereas a superficial pulse was found for heart rate ≥ 58.25 beats/min; a fast pulse was found for heart rate ≥ 69.6 beats/min and pulse wave velocity ≥ 9.185 m/s. CONCLUSION： Associations were explained by La Place＇s law, arterial remodeling in hypertension, alongside the traditional criterion for classifying speed in pulse images. PIA is associated with PWA and hemodynamics in patients with hypertension. Systolic and pulse pressures, heart rate, and pulse wave velocity are quantitative variables that have information to describe the qualitative pulse images such as strength, depth and speed.

关键词： medicine Chinese traditional hypertension syndrome differentiation rehabilitation

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Association between Infrared Thermography, Blood Count and Creatine Kinase in the Evaluation of the Welfare of Vaquejada Horses

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Open Journal of Veterinary medicine 2023年第6期13卷 53-67页

作者： Carolina Jones ferreira Lima da Silva Keity Laiane Gomes Trindade Raissa Karolliny Salgueiro Cruz César Fabiano Vilela Clarisse Simões Coelho José Dantas Ribeiro filho Helena Emília Cavalcanti da Costa Cordeiro manso Hélio Cordeiro manso filho Núcleo de Pesquisa Equina Universidade Federal Rural de Pernambuco Recife Brazil Faculdade de medicina Veterinária Centro Universitário Cesmac Maceió Brazil médico Veterinário Privado Americana Brazil Faculdade de medicina Veterinária Universidade Lusófona de Humanidades e Tecnologias Lisboa Portugal Departamento de medicina Veterinária Universidade Federal de Vi&#231osa Vi&#231osa Brazil

The Quarter-Horses regularly participate in non-traditional sports that simulate activities on cattle ranches. In the vaquejada races, the horses run at about 30 km/h, reaching maximum heart rates of around 200 bpm, indicating the great physical effort during competitions and with that could impact welfare. To test the hypothesis that vaquejada horses can be evaluated for the quality of welfare through a combination of non-invasive and invasive methods, an experiment was developed that aimed to assess the quality of welfare through thermography and blood biomarkers before and after vaquejada races. Ten vaquejada horses, which were in regular competition, were submitted to the vaquejadas racing test were used. Thermography was performed in 14 regions of interest (head, neck and thorax) in the following phases: pre-race and +1, +4 and +24 hours of the races. Blood samples were collected in pre-race, immediately after and +1, +4 and +24 hours of the races. Results were submitted to ANOVA and Tukey’s test, with p set at 5%. Thermography showed that local temperatures before the race and +24 hours after the races were similar (p > 0.05), as well as between +1 and +4 hours after the races (p > 0.05), which showed higher surface temperatures than the first group (pre-race and +24 hours) (p 0.05) after the exercises. In conclusion, the vaquejada horses evaluated by infrared thermography and blood analyses maintained the quality of well-being, with the physiological elevation of several parameters after the races and recovery in less than 24 hours after the races. Thermographic images were also able to help in the analysis of the regions of interest that have contact with harnesses, demonstrating that the use of adequate and correct equipment does not compromise animal welfare.

关键词： Exercise Equine CK Hematology Equestrian Sports Well-Being

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Biomarkers of Stress and Inflammation in Assessing the Quality of Welfare of Conditioned Vaquejada Horses

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Open Journal of Veterinary medicine 2023年第8期13卷 147-159页

作者： Keity Laiane Gomes Trindade Carolina Jones ferreira Lima da Silva Raissa Karolliny Salgueiro Cruz César Fabiano Vilela Joana de Sousa Araújo Simões Clarisse Simões Coelho José Dantas Ribeiro filho Helena Emília Cavalcanti da Costa Cordeiro manso Helio Cordeiro manso filho Núcleo de Pesquisa Equina Universidade Federal Rural de Pernambuco Recife Brazil Faculdade de medicina Veterinária Centro Universitário Cesmac Maceió Brazil médico Veterinário Privado Americana Brazil Equine Academic Division Faculty of Veterinary Medicine Lusofona University (ULusofona) Lisbon Portugal CIISA-Centre for Interdisciplinary Research in Animal Health Faculty of Veterinary Medicine University of Lisbon Lisbon Portugal Associate Laboratory for Animal and Veterinary Science (AL4AnimalS) Faculty of Veterinary Medicine University of Lisbon Lisbon Portugal mediterranean Institute for Agriculture Environment and Development Universidade de évora évora Portugal Departamento de medicina Veterinária Universidade Federal de Vi&#231osa Vi&#231osa Brazil

In recent decades, the intensity of training and equestrian competitions has significantly increased, thus the assessment of the well-being of the equine athlete has become essential in all equestrian modalities. The aim of this study was to ascertain whether equine athletes submitted to a vaquejada simulation test (VqST), comprised of three races, presented changes in blood biomarkers related to stress and health status. Fourteen healthy Quarter Horses, used as pull horses in this equestrian modality, were evaluated. Ten animals were submitted to the VqST and the remaining four were used as a control group. Blood samples were collected pre-test (during fast), immediately after, and at 1, 4 and 24 hours of recovery. The assessed blood biomarkers included cortisol, interleukin (IL)-6, IL-1β, iron, urea, creatinine, and gamma-glutamyl transferase (GGT) concentrations and results were analyzed using One Way ANOVA (time) with the SigmaStat 13.0 software. No differences between sample times were detected in both groups (p > 0.05) and no differences were found between groups (p > 0.05). The results suggest that all horses were well conditioned for the level of effort imposed by the three vaquejada races. The adaptation to physical exercise may enable the regulation of the acute response to stress in the tissues involved in the exercises, with no differences being observed in stress and health biomarkers, such as IL-6, IL-1β and cortisol. In conclusion, well-conditioned vaquejada horses exhibit a balanced regulation of biological processes, which contributes an increased athletic longevity and better quality of athletic life.

关键词： Horse Races Welfare Hormones Cortisol Interleukins Iron

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Phytochemical bioprospecting, antioxidant, antimicrobial and cytotoxicity activities of saline extract from Tithonia diversifolia(Hemsl) *** leaves

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Asian Pacific Journal of Tropical Biomedicine 2018年第5期8卷 245-253页

作者： Bruno Rafael Barboza Bárbara Rafaela da Silva Barros Bárbara de Azevedo Ramos maiara Celine de moura Thiago Henrique Napoleao maria Tereza dos Santos Correia Luana Cassandra Breitenbach barroso Coelho Iranildo José da Cruz filho Ana maria Souto maior Túlio Diego da Silva Leylianne de Cássia Rodrigues Nerys Edson Renan Barros de Santana Cláudia Sampaio de Andrade Lima Virgínia maria Barros de Lorena Cristiane moutinho Lagos de melo Department of Antibiotics Biosciences Centre Federal University of Pernambuco Department of Biochemistry Biosciences Centre Federal University of Pernambuco Center for Strategic Techmologies of the Northeast Department of Biophysics Biosciences Centre Federal University of Pernambuco Aggeu magalhaes Institute IAM/FIOCRUZ

Objective:To evaluate antimicrobial and antioxidant properties of saline extract from Tithonia diversifolia leaves by phytochemical bioprospecting,and investigate its safety against animal ***:The saline extract was prepared,with NaCl(0.15 m),by constant stirring of the dried and pulverized leaves,followed by volume reduction by *** extract was phytochemical characterized using ultra-performance liquid chromatography,and total phenol and flavonoid analysis also was *** antioxidant capacity was determined through DPPH*radical,the antimicrobial property was evaluated against standard bacteria and fungi,and the viability assays were performed against mice ***:Fifteen compounds were identified belonging to two main classes terpenoids and *** extract showed 22.185 mg GAE/g of total phenolic compounds and 3.220 mg QE/g of ***,extract showed higher antioxidant ability similar to butylated hydroxytoluene a standard molecule[(3.042±0.019)mg AAE/g and(4.12±0.10)mg AAE/g to saline extract and butylated hydroxytoluene,respectively].The antimicrobial assays demonstrated that the extract had a significant antifungal potential against Candida species and could be used with safety against mice splenocytes,in concentrations lower than 50μg/mL,promoting higher proliferation in these ***:Saline extract from Tithonia diversifolia leaves presents potential antioxidant,antifungal properties and induces immunostimulation in mice splenocytes.

关键词： Tithonia diversifolia Extract Antioxidant Antifungal properties Immunostimulation

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