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无可见骨病变的多发性骨髓瘤病人腰椎骨小梁微结构分析的初步结果

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国际医学放射学杂志 2011年第5期 487-487页

作者： m.Takasu C.Tani m.Ishikawa S.Date J.Horiguchi m.kiguchi 蔡兆诚 Department of Diagnostic Radiology Graduate School of Biomedical SciencesHiroshima University1-2-3 KasumiMinamikuHiroshima-shi 734-8551Japan

目的使用多层CT分析并鉴别无脊柱骨折的多发性骨髓瘤病人与对照者的腰椎骨小梁结构,并评价其准确性。材料与方法本研究为单中心回顾性研究,获得机构审查

关键词：多发性骨髓瘤骨小梁微结构 CT 骨髓瘤浆细胞瘤病人腰椎

Physiological functions of hemocytes newly emerged from the cultured hematopoietic organs in the silkworm, Bombyx moil

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Insect Science 2010年第1期17卷 7-20页

作者： Cheng-Long Wang Zhi-Xiang Wang michael m. Kariuki Qing-Zhi Ling Kenji kiguchi Er-Jun Ling Research Centre for Insect Sciences Shanghai Institute of Plant Physiology and Ecology Chinese Academy of Sciences Shanghai China Department of Veterinary Pathobiology College of Veterinary Medicine University of Missouri Columbia MO 65211 USA Department of Applied Biology Zhejiang Pharmaceutical College Ningbo Zhejiang Province China Laboratory of Silkworm Physiology Department of Applied Biology Faculty of Textile Science and Technology Shinshu University Ueda Nagano 386-8567 Japan

Cellular immunity is a very important part of insect innate immunity. It is not clear if hemocytes entering the hemolymph require a maturation process to become competent. The establishment of a tissue culture system for the insect hematopoietic organs would enable physiological function assays with hemocytes newly emerged from hematopoietic organs. To this end, we established a hematopoietic organ culture system for the purebred silkworm pnd pS and then studied the physiological functions of the newly emerged hemocytes. We found that Grace＇s medium supplemented with 10% heated silkworm larval plasma was better for culturing the hematopoietic organs ofpndpS. Newly emerged hemocytes phagocytosed propidium iodide-labeled bacteria and encapsulated the Iml-2 coated nickel beads as well as pupal tissue debris. This culture system is therefore capable of generating physiologically functional hemocytes. These hemocytes can be used to study the mechanisms of the hemocyte immune response among others.

关键词： Bombyx mori encapsulation hemocyte hematopoietic organ phagocytosis tissue culture

<i>Lef</i>1 may contribute to agenesis of the third molars in mice

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Open Journal of Stomatology 2013年第5期3卷 281-286页

作者： Takehiko Shimizu Takahiro Ichinosawa Yuri kiguchi Fusae Ishida Takahide maeda Department of Pediatric Dentistry Nihon University School of Dentistry at Matsudo Chiba Japan

Tooth agenesis is the most common developmental anomaly of the human dentition. Epilepsy-like disorder (EL) mice, which have a 100% incidence of agenesis of the third molars, may be a good model for the genetic study of human tooth agenesis. Our previous congenic breeding strategy using EL mice confined a major locus for agenesis of m3, designated am3, within an approximately 1 mega base pair (mbp) interval on chromosome 3, which contains five known genes;Lef1, Hadh, Cyp2u1, Sgms2 and Papss1. The aim of this study was to identify the strongest candidate for am3 among the five genes using real-time PCR analysis. The tooth germs of m3 in the bud stage of EL and control mice were dissected out, and total RNA was extracted. In real-time PCR analysis, a significantly low level of expression of Lef1, which is one of the essential transcription factors for early tooth development, was observed in m3 of EL mice. In addition, a significantly low level of expression of Fgf4, which is a direct transcriptional target for LEF1 in early tooth development, was observed in m3 of EL mice. Our results suggest that the cause of m3 agenesis of EL mice may be a low level of Lef1 expression in m3 in the bud stage of EL mice.

关键词： Hypodontia Gene Expression EL mice

前瞻性和回顾性心电门控64层CT冠状动脉成像影像质量,血管狭窄和放射剂量评估的对照性研究

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国际医学放射学杂志 2008年第5期31卷 399-400页

作者： N. Hirai J. Horiguchi C. Fujioka m. kiguchi H. Yamamoto N.matsuura 邱建星（译）唐光健（校） Department of Clinical Radiology Hiroshima University Hospital 1-2-3 Kasumi-cho Minami-ku Hiroshima 734-8551 Japan. 不详

目的证实前瞻性心电图（ECG）触发冠状动脉CT血管成像（下称前瞻性cT血管成像）和回顾性ECG门控冠状动脉CT血管成像（下称回顾性CT血管成像）同样有效。方法研究得到机构审查委员会的批准并获得知情同意书。60例心率低于75次/min且需行... 详细信息

目的证实前瞻性心电图（ECG）触发冠状动脉CT血管成像（下称前瞻性cT血管成像）和回顾性ECG门控冠状动脉CT血管成像（下称回顾性CT血管成像）同样有效。方法研究得到机构审查委员会的批准并获得知情同意书。60例心率低于75次/min且需行冠状动脉CT血管成像的病人入组。使用64层CT进行了前瞻性和回顾性CT血管成像。记录数据采集时间。2位独立心脏影像诊断医师使用美国心脏协会17段分段法，对影像质量进行主观评估（1为优秀，4为差），并评估血管的狭窄程度（0％狭窄，1％～49％狭窄，50％-75％狭窄，〉75％狭窄）。

关键词：冠状动脉成像回顾性心电门控血管狭窄 64层CT 影像质量剂量评估 CT血管成像美国心脏协会

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黑化诱导激素对白薯天蛾的发育影响(英文)

激光生物学报 2012年第1期21卷 27-30页

作者：孔璐璐李旭全路岸瑞杨兵木口憲爾凌尔军刘朝良安徽农业大学生命科学学院安徽合肥230036 中科院上海生命科学研究院植物生理生态研究所上海200032 日本信州大学纤维学部应用生物科学科长野3868567

黑化诱导激素是一种神经肽,它像激素一样调控昆虫的生长发育。给五龄白薯天蛾注射较高浓度的黑化诱导激素时,它们的吐丝期明显延长,蛹重也明显增加。给五龄各天白薯天蛾注射136 pmol每克体重的黑化诱导激素,它们的添食期、吐丝期延长,... 详细信息

黑化诱导激素是一种神经肽,它像激素一样调控昆虫的生长发育。给五龄白薯天蛾注射较高浓度的黑化诱导激素时,它们的吐丝期明显延长,蛹重也明显增加。给五龄各天白薯天蛾注射136 pmol每克体重的黑化诱导激素,它们的添食期、吐丝期延长,蛹重增加。因此,黑化诱导激素会延长天蛾幼虫的添食期和吐丝期,影响天蛾幼虫的发育。

关键词：黑化诱导激素白薯天蛾吐丝期添食期蛹重

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