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Purification and Biochemical Characterization of N-methyl-histidylesterase from Camel （Camelus dromedarius） Liver

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Journal of Chemistry and Chemical Engineering 2011年第8期5卷 671-683页

作者： manal h. A. Ahmad Ghaleb m. Abuerreish Department of Biological Sciences Faculty of Science University of Jordan Amman 11942 Jordan

Camel Liver Esterase （LE） was isolated through five consecutive steps： Extraction with Tris/hCl buffer, ph 8, precipitation with ammonium sulfate, affinity chromatography on Affi-gel-butyric acid and on Affi-gel-oleic acid, and preparative polyacrylamide gel electrophoresis （PAGE）. The Km values were found as： methyl butyrate （8.3 mm）, α-naphthyl acetate （3.65 mm）, 13-naphthyl myristate （66.7 mm）, p-nitrophenyl acetate （0.29 mm）, and phenyl acetate （5.26 mm）. The Ki of LE inhibition by bis（4-nitrophenyl） phosphate was 7.9 p.m, and 58 μm by phenyl-methyl-sulfonyl fluoride. The inhibition by these inhibitors is irreversible. p-hydroxymercuribenzoate or ethylenediamine-tetra-acetic acid did not inhibit the enzyme. LE showed a dimeric structure with molecular weight of 129 kD. The energy of activation of LE was 15.0, 5.5 and 10.75 Kcal, using the substrates： a-naphthyl acetate, p-nitrophenyl acetate, and methyl butyrate, respectively. The optimal ph for LE was between 8 and 10. The N-terminus was found as aspartic acid. The percentage of glycine residues （13.3%） was the highest whereas the percentage of cysteine residues （0.68%） was the lowest in LE. Amino acid composition shows that LE has -50% of its histidine residues as N-methylhistidines.

关键词： Camel. Camelus dromedarius Carboxyl esterase Esterase methylhistidyl esterase methyl-histidine Liver esterase

Comparison of different magnetic resonance imaging sequences for assessment of fistula-in-ano

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World Journal of Radiology 2014年第5期6卷 203-209页

作者： michael R Torkzad hkan Ahlstrm Urban Karlbom 12 Reris Grange Close Milford SurreyGU8 5FD United Kingdom Section of Radiology Section Department of Radiology Oncology and Radiation Science Uppsala University SE 75185 Uppsala Sweden Department of Surgery Uppsala University Hospital SE 75185 Uppsala Sweden

AIm: To assess agreement between different forms of T2 weighted imaging(T2WI), and post-contrast T1WI in the depiction of fistula tracts, inflammation, and internal openings with that of a reference test. mEThODS: Thirty-nine consecutive prospective cases were enrolled. The following sequences were used for T2WI: 2D turbo-spin-echo(2D T2 TSE); 3D T2 TSE; short tau inversion recovery(STIR); 2D T2 TSE with fat saturation performed in all patients. T1WI were either a 3D T1-weighted prepared gradient echo sequence with fat saturation or a 2D T1 fat saturation [Spectral presaturation with inversion(SPIR)]. Agreement for each sequence for determination of fistula extension, internal openings, and the presence of active inflammation was assessed separately and blindly against a reference test comprised of follow-up, surgery, endoscopic ultrasound, and assessment by an independent experienced radiologist with access to all ***: Fifty-six fistula tracts were found: 2 intersphincteric, 13 trans-sphincteric, and 24 with additional tracts. The best T2 weighted sequence for depiction of fistula tracts was 2D T2 TSE(Cohen's kappa = 1.0), followed by 3D T2 TSE(0.88), T2 with fat saturation(0.54), and STIR(0.19). Internal openings were best seen on 2D T2 TSE(Cohen's kappa = 0.88), followed by 3D T2 TSE(0.70), T2 with fat saturation(0.54), and STIR(0.31). Detection of inflammation showed Cohen's kappa of 0.88 with 2D T2 TSE, 0.62 with 3D T2 TSE, 0.63 with STIR, and 0.54 with T2 with fat saturation. STIR, 3D T2 TSE, and T2 with fat saturation did not make any contributions compared to 2D T2 TSE. Post-contrast 3D T1 weighted prepared gradient echo sequence with fat saturation showed better agreement in the depiction of fistulae(Cohen's kappa = 0.94), finding internal openings(Cohen's kappa = 0.97), and evaluating inflammation(Cohen's kappa = 0.94) compared to post-contrast 2D T1 fat saturation or SPIR where the corresponding figures were 0.71, 0.66, and 0.87, respectively.

关键词： Fistula magnetic resonance imaging Diagnosis

Sugarcane bagasse amendment mitigates nutrient leaching from a mineral soil under tropical conditions

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Pedosphere 2022年第6期32卷 876-883页

作者： Nan XU Jehangir h.BhADhA Abul RABBANY Stewart SWANSON James mmCCRAY Yuncong LI Sarah LSTRAUSS Rao mYLAVARAPU horticultural Sciences Department University of FloridaGainesville FL 32611(USA) Everglades Research and Education Center University of FloridaBelle Glade FL 33430(USA) Department of Soil Waterand Ecosystem SciencesUniversity of FloridaGainesville FL 32611(USA) Institute of Food and Agricultural Sciences(IFAS)Extension University of FloridaLaBelle FL 33935(USA) Agronomy Department University of FloridaBelle Glade FL 33430(USA) Department of Soil Waterand Ecosystem SciencesUniversity of FloridaHomestead FL 33031(USA) Department of Soil Waterand Ecosystem SciencesUniversity of FloridaImmokalee FL 34142(USA)

Large quantities of organic by-products are generated by the sugarcane industry during sugar extraction *** by-products may be used as soil amendments to improve soil quality,as nutrient leaching is common in mineral soils of Florida in USA due to their sandy texture and frequent rain events.A soil column study was designed to evaluate the effects of bagasse application at 85 t ha^(-1) of fresh bagasse,170 t ha^(-1) of fresh bagasse,and 170 t ha^(-1) of fresh bagasse+nitrogen(N)on the leaching potential of carbon(C),N,phosphorus(P),and potassium(K).Bagasse was incorporated within the topsoil(0-15 cm)in outdoor soil columns exposed to natural conditions,with periodical irrigation during the *** approximately 57 weeks,the distributions of C,N,P,and K were evaluated at three soil depths(i.e.,0-15,15-30,and 30-53 cm)within the soil *** organic C(TOC),N,and P in leachates decreased significantly from the soils amended with bagasse compared with the control with no bagasse and no *** on K content changes in the columns,bagasse could also be utilized as a potential source of K for ***,application of bagasse as a soil amendment might be an effective way to sustainably reutilize organic by-products while reducing concerns regarding major nutrients entering groundwater *** results of this study could assist in developing nutrient management plans of using sugarcane bagasse as a potential soil amendment in mineral soils.

关键词： nutrient leaching potential nutrient loss pore volume sandy soil soil amendment soil column sugarcane by-product

Geochemical Siliceous and Silicified Facies of Phosphate Series of Ouled Abdoun Basin (morocco)

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Open Journal of Geology 2014年第7期4卷 295-302页

作者： h. El haddi A. Benbouziane m. mouflih Laboratory of Dynamics Sedimentary Basins and Geological Correlation Faculty of Sciences Ben M’sik Hassan II Mohammedia-Casablanca University Casablanca Morocco

Syn-sedimentary post-diagenetic phenomena are remarkable in moroccan phosphates. Indeed they are reference deposits for geological study, where important interests are given of determination and identification of these geochemical phenomena. Silicification is one of the phenomena that we have studied to determine the diagenetic processes that control the new-formation of silica in phosphate series of Ouled Abdoun. This study was performed on deposit EL halassa, which belongs to the Ouled Abdoun Basin;they spread out the maastrichtian to Lutetian. At first, petrographic approach was applied on different terms composing the phosphate series. In a second step, a geochemical approach on the same silica and silicified facies of phosphate series Ouled Abdoun Basin for characterizing silicification deduced the probable links between this diagenetic phenomenon and facies and forms’ affect. Ten major trace elements were measured. According to the phases’ petrographic relationship, the results were presented and discussed: silicate phase, the carbonate phase and clay phase.

关键词： Phosphates Diagenesis Silicification Ouled Abdoun morocco

Design and Analysis of a Compact Band Notch UWB Antenna for Body Area Network

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Journal of Electromagnetic Analysis and Applications 2018年第9期10卷 157-169页

作者： h. m. Arifur Rahman mohammad monirujjaman Khan Department of Electrical & Computer Engineering School of Engineering & Physical Science North South University Bashundhara Dhaka

This paper presents the design of a small printed ultra wideband antenna with Band Notched characteristics. Both the free space and on-body performances of this antenna were investigated through simulation. The newly designed UWB antenna is more revised small form factor sized, with the ability to avoid interference caused by WLAN (5.15 - 5.825 Ghz) and WimAX (5.25 - 5.85 Ghz) systems with a band notch. The return loss response, gain, radiation pattern on free space of the antenna were investigated. After that, the on-body performances were tested on 3-layer human body model with radiation pattern, gain, return loss, and efficiency at 3.5, 5.7, 8, 10 Ghz and all the results were compared with free space results. As the on-body performance was very good, the proposed antenna will be suitable to be used for multi-purpose medical applications and sports performance monitoring.

关键词： Ultra Wide Band Antenna Small Form Factor Antenna Body Area Networks Band Notch Printed Antenna Wireless Body Area Network multiple Slot Antenna

Coarse corn particles cause a negative effect on eggshell quality of semi-heavy laying hens

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Animal Nutrition 2019年第4期5卷 432-434页

作者： Vanessa R.m.Oliveira Alex m.V.Arruda Aurora S.melo Davyd h.Souza Joao B.F.Souza-Junior Raimunda T.V.Fernandes Joao P.A.F.Queiroz Department of Animal Sciences Universidade Federal Rural do Semi-AridoMossoro 59625900Brazil Department of Animal Science Federal University of CearaFortalezaCEP 60455-760Brazil

One of the possible ways to optimize the productive performance of poultry is through the physical processing of ingredients,which can improve the use of nutrients in these *** this context,this study was to evaluate the effects of different corn particle sizes in diets on the productive performance and egg quality of semi-heavy laying *** naked neck laying hens were used from 24 weeks of age and distributed in a completely randomized *** diets in different treatments contained corn grain ground into sieves with a diameter of 2,4,or 8 mm to provide corn particles with a geometric mean diameter(GmD) of 605(fine),1,030(medium),and 2,280 μm(coarse),*** feed and leftovers were weighed daily throughout the experimental period to determine the feed intake and feed *** corn particle sizes did not affect any aspect of the productive performance of hens,except for feed *** fed fine and medium corn particles exhibited higher values for egg yolk color,eggshell weight,and eggshell *** is suggested that semi-heavy laying hens should be fed mash diets containing corn particles with GmD from 605 to 1,030 μm,because coarse corn particles cause a negative effect on eggshell quality.

关键词： Egg quality Geometric mean diameter Productive performance Poultry nutrition

X-Raying Rainfall Pattern and Variability in Northeastern Nigeria: Impacts on Access to Water Supply

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Journal of Water Resource and Protection 2010年第11期2卷 952-959页

作者： h. T. Ishaku m. Rafee majid 不详

This paper is premised on the hypothesis that the amount of rainfall in Nigeria decreases with increasing distance from the coastal areas to the north semi arid lands. This belief widely held in some circles does not really follow this pattern due to other climatic factors. This paper examined rainfall pattern and its variability in northeastern Nigeria and its impacts on access to water supply. Data on the mean monthly rainfall over a period of 33 years (1970-2002) were collected from the Nigeria meteorological Agency (NImET) in Abuja, Nigeria. The result of the analysis indicates that the mean annual rainfall in the study area is not dependent on distance but some other climatic factors such as relief, solar radiation, temperature, winds, and nature of soil among others. Thus, the amount of rainfall received in Taraba and Borno states which are located in the southern most and extreme end of the study area respectively were higher than that of Gombe state which lies in between them.

关键词： Rainfall Pattern Variability Impact Access to Water Northeastern Nigeria

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营养对家禽先天免疫反应的影响

中国家禽 2010年第16期32卷 35-37页

作者： m.h.Kogut 许明(译) 美国农业部农业研究服务署南部平原研究中心不详

传染病一直是家禽业生产的一种威胁，如何阻止并控制传染病持续受到人们的关注。在动物生产中，由于外来和新发传染病的持续威胁以及对抗生素使用的日益关注，需要寻找更加实用的替代措施控制传染病。目前世界范围内减少致病菌广泛采用... 详细信息

传染病一直是家禽业生产的一种威胁，如何阻止并控制传染病持续受到人们的关注。在动物生产中，由于外来和新发传染病的持续威胁以及对抗生素使用的日益关注，需要寻找更加实用的替代措施控制传染病。目前世界范围内减少致病菌广泛采用的有两种方法，即免疫干扰措施和治疗用抗菌剂。日粮中的生物活性成分与免疫反应的互作可降低动物对传染病的易感性，

关键词：免疫反应家禽业营养动物生产生物活性成分传染病替代措施世界范围

DFT Study of Poly Furfuryl Alcohol-Rhodamine B Blend

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Advances in Chemical Engineering and Science 2014年第2期4卷 161-166页

作者： Aqeel m. Ali Ali h. Al-mowali Department of Chemistry College of Science University of Basrah Basrah Iraq molecular Physics Lab (mPL) Department of Physics College of Science University of Basrah Basrah Iraq

A theoretical study of poly furfuryle alcohol (PFA), rhodamine B (Rh B) dye and their blends (PFA-Rh B) is carried out by using the density functional theory (DFT). Electronic states and opto-electronic properties are investigated. The electronic states indicate that the oligomers of FA are insulators and Rh B is a wide band gap semiconductor. Their blends have a narrow band gap of about 0.75 eV. The optoelectronic properties are studied using TDDFT, which indicates that the chain length of poly furfuryl alcohol is an effective parameter to control both energies and intensities of absorption in which longer chain causes absorption with high intensity within long wavelength. A single broad baned of electron excitations is more like 8-oligomer-RhB case, which centers at wavelengths about 599 nm and 625 nm. This band of absorption covers the whole visible region of spectrum.

关键词： Poly Furfuryle Alcohol Rhodamine B DFT Electron Excitations