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A Glimpse into the Natural History of Leiolepis rubritaeniata mertens,1961(Squamata:Leiolepididae)

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Asian Herpetological Research 2023年第2期14卷 147-155页

作者： gunther KÖHLER Panupong THAmmACHOTI Senckenberg Forschungsinstitut und Naturmuseum Senckenberganlage 25Frankfurt a.M.60325Germany Department of Biology Faculty of ScienceChulalongkorn UniversityBangkok 10330Thailand

During the months of April through July 2020 we studied aspects of the natural history of Leiolepis rubritaeniata,a species of butterfly lizard that occurs on the Khorat Plateau in Thailand and adjacent regions of Laos and Cambodia as well as in south-central *** present data on population size and structure,as well as location,size,compass orientation,and structure of the lizard’s ***,we present climatic data(ambient air temperature and precipitation)at the study site for the duration of our field work as well as temperature data for the complete year ***,we provide data on egg laying,incubation conditions and characteristics of the ***,we present an easy to use and reliable non-invasive method for the long-term recognition of individual butterfly lizards based on their unique dorsal *** lizards are utilized as a food source for the local human *** far,there are no farming projects in Thailand involving this species and all individuals are collected from the wild *** data are available on the population dynamics of *** but it can be assumed that habitat destruction due to land use change as well as its utilization for human consumption have negative effects on the long-term survival of the local populations of this lizard *** baseline data presented here are essential for any meaningful conservation strategy for these lizards.

关键词： Burrow structure butterfly lizard ecology Khorat Plateau Southeast Asia Squamata

Characterization of different plasma-treated cobalt oxide catalysts for oxygen reduction reaction in alkaline media

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Science Bulletin 2016年第8期61卷 612-618页

作者： Lisa m.Uhlig Gustav Sievers Volker Brüser Alexander Dyck gunther Wittstock NEXT ENERGY EWE-Research Centre for Energy Technology26129 OldenburgGermany Faculty of mathematics and Science CIS-Center of Interface ScienceInstitute of ChemistryCarl von Ossietzky University of Oldenburg26111 OldenburgGermany Leibniz Institute for Plasma Science and Technology 17489 GreifswaldGermany

Plasma-synthesized cobalt oxide supported on carbon has been analyzed for its use for electrocatalytic oxygen reduction reaction(ORR) in alkaline anion exchange membrane fuel cells(AEmFC). This work presents the ORR activity in 0.1 mol L^(-1) KOH and 0.1 mol L^(-1) K_2CO_3at25 °C. Cyclic voltammetry(CV) was used to determine the potentials at which the ORR occurs and to evaluate the stability of catalyst. moreover, a rotating ring-disk electrode(RRDE) was used to investigate the activity of the catalysts and the formation of the by-product hydroperoxide anion(HO2-) as well as to identify the preferred pathway of the ORR. Calculated kinetic parameters for the ORR for the cobalt catalysts are shown in this work together with a comparison to a commercial platinum catalyst. However, the cobalt oxide produced more by-products which could lead to damage of the membrane in a fuel cell through a radical attack of the polymer backbone.

关键词：钴催化剂碱性介质等离子体处理氧化钴阴离子交换膜表征旋转环盘电极燃料电池

AmmONIA DISPERSION FROm mULTI-FLOOR VERSUS STANDARD SINGLE-FLOOR PIG PRODUCTION FACILITIES BASED ON COmPUTATIONAL FLUID DYNAmICS SImULATIONS

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Frontiers of Agricultural Science and Engineering 2023年第3期10卷 374-389页

作者： Yicong XIN Li RONG gunther SCHAUBERGER Dejia LIU Xiusong LI Zhihua YANG Songming ZHU Dezhao LIU Key Laboratory of Equipment and Informatization in Environment Controlled Agriculture Ministry of Agriculture and Rural AffairsKey Laboratory of Intelligent Equipment and Robotics for Agriculture of Zhejiang ProvinceInstitute of Agricultural Bio-Environmental EngineeringCollege of Biosystems Engineering and Food ScienceZhejiang UniversityHangzhou 310058China Department of Civil and Architectural Engineering—Building Science Aarhus UniversityAarhus C 8000Denmark WG Environmental Health Unit for Physiology and BiophysicsUniversity of Veterinary MedicineA-1210 ViennaAustria Big Herdsman machinery Co. Ltd.Qingdao 266000China Zhejiang Huatong meat Products Co. Ltd.Yiwu 322000China

multi-floor buildings for raising pigs have recently attracted widespread attention as an emerging form of intensive livestock production especially in eastern China,due to the fact that they can feed a much larger number of animals per unit area of land and thus alleviate the shortage of land available for standard single-floor pig production ***,this more intensive kind of pig building will pose new challenges to the local environment in terms of pollutant *** compare the dispersion air pollutants(ammonia as a representative)emitted from multi-versus single-floor pig buildings,ammonia dispersion distance and concentration gradients were investigated through three-dimensional simulations based on computational fluid *** validation of an isolated cubic model was made to ensure the simulation method was *** effects of wind direction,wind speed and emission source concentration at 1.5 m(approximate human inhalation height)during summer were *** results showed that the ammonia dispersion distance of the multi-floor pig building was far greater than that of the single-floor building on a plane of Z=1.5 *** the wind direction was 67.5°,the wind speed was 2 m·s^(−1) and the emission source concentration was 20 ppmv,the dispersion distance of the multi-floor pig building could reach 1380 ***,the ammonia could accumulate in the yard to 7.68 ***,future site selection,wind speed and source concentration need to be given serious *** on the simulation used in this study with source concentration is 20 ppmv,the multi-floor pig buildings should be located 1.4 km away from residential areas to avoid affecting *** results of this study should guidance for any future development of multi-floor pig buildings.

关键词： pig building computational fluid dynamics ammonia dispersion

Can Quick Release Experiments Reveal the muscle Structure？ A Bionic Approach

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Journal of Bionic Engineering 2012年第2期9卷 211-223页

作者： D. F. B. Haeufle m. gunther R. Blickhan S. Schmitt Institute of Sports and Exercise-Science University of Stuttgart Allmandring 28 D-70569 Stuttgart Germany Stuttgart Research Centre for Simulation Technology （SRC SimTech） University of Stuttgart Pfaffenwaldring 7a D-70569 Stuttgart Germany Institute of motion Science Friedrich-Schiller-University Seidelstraβe 20 D-07749 Jena Germany

The goal of this study was to understand the macroscopic mechanical structure and function of biological muscle with respect to its dynamic role in the contraction. A recently published muscle model, deriving the hyperbolic force-velocity relation from first-order mechanical principles, predicts different force-velocity operating points for different load situations. With a new approach, this model could be simplified and thus, transferred into a numerical simulation and a hardware experiment. Two types of quick release experiments were performed in simulation and with the hardware setup, which represent two extreme cases of the contraction dynamics： against a constant force （isotonic） and against an inertial mass. Both experiments revealed hyperbolic or hyperbolic-like force-velocity relations. Interestingly, the analytical model not only predicts these extreme cases, but also additionally all contraction states in between. It was possible to validate these predictions with the numerical model and the hardware experiment. These results prove that the origin of the hyperbolic force-velocity relation can be mechanically explained on a macroscopic level by the dynamical interaction of three mechanical elements. The implications for the interpretation of biological muscle experiments and the realization of muscle-like bionic actuators are discussed.

关键词： force-velocity relation isotonic quick release proof of concept artificial muscle

Plant Phosphatidylcholine-Hydrolyzing Phospholipases C NPC3 and NPC4 with Roles in Root Development and Brassinolide Signaling in Arabidopsis thaliana

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molecular Plant 2010年第3期3卷 610-625页

作者： Rinukshi Wimalasekera Premysl Pejchar Andre Holka Jan martinec gunther RE. Scherer Leibniz University of Hannover Institute of Floriculture and Wood Science Section of Applied Molecular Physiology Herrenhauser Strasse 2 D-30419Hannover Germany Institute of Experimental Botany Academy of Sciences of the Czech Republic v.v.i. Rozvojova 263 165 02 Prague 6 Czech Republic

Phosphatidylcholine-hydrolyzing phospholipase C （PC-PLC） catalyzes the hydrolysis of phosphatidylcholine （PC） to generate phosphocholine and diacylglycerol （DAG）. PC-PLC has a long tradition in animal signal transduction to generate DAG as a second messenger besides the classical phosphatidylinositol splitting phospholipase C （PI-PLC）. Based on amino acid sequence similarity to bacterial PC-PLC, six putative PC-PLC genes （NPC1 to NPC6） were identified in the Arabidopsis genome. RT-PCR analysis revealed overlapping expression pattern of NPC genes in root, stem, leaf, flower, and silique. In auxin-treated PNPc3：GUS and PNPc4：GUS seedlings, strong increase of GUS activity was visible in roots, leaves, and shoots and, to a weaker extent, in brassinolide-treated （BL） seedlings. PNPc4：GUS seedlings also responded to cytokinin with increased GUS activity in young leaves. Compared to wild-type, T-DNA insertional knockouts npc3 and npc4 showed shorter primary roots and lower lateral root density at low BL concentrations but increased lateral root densities in response to exogenous 0.05-1.0 I^m BL BL-induced expression of TCH4 and LRX2, which are involved in cell expansion, was impaired but not impaired in repression of CPD, a BL biosynthesis gene, in BL-treated npc3 and npc4. These observations suggest NPC3 and NPC4 are important in BL-mediated signaling in root growth. When treated with 0.1 I^m BL, DAG accumulation was observed in tobacco BY-2 cell cultures labeled with fluorescent PC as early as 15 min after application. We hypothesize that at least one PC-PLC is a plant signaling enzyme in BL signal transduction and, as shown earlier, in elicitor signal transduction.

关键词： Auxin brassinolide signaling phosphate deficiency phosphatidylcholine-splitting phospholipase C (PC-PLC) NPC genes.

Patterns and Timing in Expression of Early Auxin-Induced Genes Imply Involvement of Phospholipases A （pPLAs） in the Regulation of Auxin Responses

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molecular Plant 2013年第5期6卷 1473-1486页

作者： Corinna Labusch maria Shishova Yunus Effendi maoyin Li Xuemin Wang gunther RE. Scherera Leibniz Universit&t Hannover Institut for Zierpflanzenbau und Geh61zwissenschaften Abt. Molekulare Ertragsphysiologie Herrenhauser Str. 2 D-30419 Hannover Germany Department of Plant Physiology and Biochemistry State University of St. Petersburg Universitetskaya em. 7/9 St. Petersburg 199034 Russia Department of Biology University of Missouri St Louis MO 63121 USA Donald Danforth Plant Science Center St Louis MO 63132 USA

While it is known that patatin-related phospholipase A （pPLA） activity is rapidly activated within 3min by auxin, hardly anything is known about how this signal influences downstream responses like transcription of early auxin-induced genes or other physiological responses. We screened mutants with T-DNA insertions in members of the pPLA gene family for molecular and physiological phenotypes related to auxin. Only one in nine Arabidopsis thaliana ppla knockdown mutants displayed an obvious constitutive auxin-related phenotype. Compared to wild-type, ppla-IIlδ mutant seedlings had decreased main root lengths and increased lateral root numbers. We tested auxin-induced gene expression as a molecular readout for primary molecular auxin responses in nine ppla mutants and found delayed up- regulation of auxin-responsive gene exRression in all of themL Thirty minutes after auxin treatment, up-regulation of up to 40% of auxin-induced genes was delayed in mutant seedlings. We observed only a few cases with hypersensitive auxin-induced gene expression in ppla mutants. While, in three ppla mutants, which were investigated in detail, rapid up- regulation （as early as 10 min after auxin stimulus） of auxin-regulated genes was impaired, late transcriptional responses were wild-type-like. This regulatory or dynamic phenotype was consistently observed in different ppla mutants with delayed up-regulation that frequently affected the same genes. This defect was not affected by pPLA transcript levels which remained constant. This indicates aposttranslational mechanism as a functional link of pPLAs to auxin signaling. The need for a receptor triggering an auxin response without employing transcription regulation is discussed.

关键词： hormonal regulation hormone biology signal transduction.

Real-time high-resolution mid-infrared optical coherence tomography

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Light(Science & Applications) 2019年第1期8卷 1076-1088页

作者： Niels m.Israelsen Christian R.Petersen Ajanta Barh Deepak Jain mikkel Jensen gunther Hannesschlager Peter Tidemand-Lichtenberg Christian Pedersen Adrian Podoleanu Ole Bang DTU Fotonik Technical University of Denmark2800 KgsLyngbyDenmark NORBLIS IVS Virumgade 35 DVirum 2830Denmark Research Center for Non-Destructive Testing(RECENDT) Altenberger Straße 694040 LinzAustria NLIR ApS Hirsemarken 1Farum 3520Denmark Applied Optics Group School of Physical SciencesUniversity of KentCanterbury CT27NHUK

The potential for improving the penetration depth of optical coherence tomography systems by using light sources with longer wavelengths has been known since the inception of the technique in the early ***,the development of mid-infrared optical coherence tomography has long been challenged by the maturity and fidelity of optical components in this spectral region,resulting in slow acquisition,low sensitivity,and poor axial *** this work,a mid-infrared spectral-domain optical coherence tomography system operating at a central wavelength of 4μm and an axial resolution of 8.6μm is *** system produces two-dimensional cross-sectional images in real time enabled by a high-brightness 0.9-to 4.7-μm mid-infrared supercontinuum source with a pulse repetition rate of 1 mHz for illumination and broadband upconversion of more than 1-μm bandwidth from 3.58–4.63μm to 820–865 nm,where a standard 800-nm spectrometer can be used for fast *** images produced by the mid-infrared system are compared with those delivered by a state-of-the-art ultra-high-resolution near-infrared optical coherence tomography system operating at 1.3μm,and the potential applications and samples suited for this technology are *** doing so,the first practical mid-infrared optical coherence tomography system is demonstrated,with immediate applications in real-time non-destructive testing for the inspection of defects and thickness measurements in samples that exhibit strong scattering at shorter wavelengths.

关键词： resolution repetition illumination

Bright betatron radiation from direct-laseraccelerated electrons at moderate relativistic laser intensity

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matter and Radiation at Extremes 2021年第4期6卷 10-17页

作者： O.N.Rosmej X.F.Shen A.Pukhov L.Antonelli F.Barbato m.Gyrdymov m.m.gunther S.Zahter V.S.Popov N.G.Borisenko N.E.Andreev GSI Helmholtzzentrum fur Schwerionenforschung GmbH Planckstraße164291 DarmstadtGermany Goethe University FrankfurtMax-von-Laue-Straße 160438 Frankfurt am MainGermany Helmholtz Forschungsakademie Hessen fur FAIR(HFHF) Campus Frankfurt am MainMax-von-Laue-Straße 1260438 Frankfurt am MainGermany Heinrich-Heine-University Dusseldorf Universitatsstraße 1DusseldorfGermany York Plasma Institute University of YorkChurch LaneHeslingtonYork YO105DQUnited Kingdom University of Bordeaux CNRSCEACELIAUMR 5107F-33405 TalenceFrance Joint Institute for High Temperatures RASIzhorskaya St.13Bldg.2125412 MoscowRussia moscow Institute of Physics and Technology(State University) Institutskiy Pereulok 9141700 DolgoprudnyMoscow RegionRussia P.N.Lebedev Physical Institute RASLeninsky Prospekt 53119991 MoscowRussia

Direct laser acceleration(DLA)of electrons in a plasma of near-critical electron density(NCD)and the associated synchrotron-like radiation are discussed for moderate relativistic laser intensity(normalized laser amplitude a0≤4.3)and ps length *** regime is typical of kJ PW-class laser facilities designed for high-energy-density(HED)*** experiments at the PHELIX facility,it has been demonstrated that interaction of a 1019 W/cm2 sub-ps laser pulse with a sub-mm length NCD plasma results in the generation of high-current well-directed superponderomotive electrons with an effective temperature ten times higher than the ponderomotive potential[Rosmej et al.,Plasma *** Fusion 62,115024(2020)].Three-dimensional particle-in-cell simulations provide good agreement with the measured electron energy distribution and are used in the current work to study synchrotron radiation from the DLA-accelerated *** resulting x-ray spectrum with a critical energy of 5 keV reveals an ultrahigh photon number of 731011 in the 1–30 keV photon energy range at the focused laser energy of 20 *** simulations of betatron x-ray phase contrast imaging based on the DLA process for the parameters of a PHELIX laser are *** results are of interest for applications in HED experiments,which require a ps x-ray pulse and a high photon flux.

关键词： intensity relativistic critical

RIG-I immunotherapy overcomes radioresistance in p53-positive malignant melanoma

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分子细胞生物学报:英文版 2023年第1期15卷 13-26页

作者： Silke Lambing Yu Pan Tan Paraskevi Vasileiadou Stefan Holdenrieder Patrick müller Christian Hagen Stephan Garbe Rayk Behrendt martin Schlee Jasper G.van den Boorn Eva Bartok marcel Renn gunther Hartmann Institute of Clinical Chemistry and Clinical Pharmacology University Hospital BonnBonn 53127Germany Institute of Laboratory medicine German Heart CentreMunich 80636Germany Department of Radiation Oncology University Hospital BonnBonn 53127Germany Unit of Experimental Immunology Department of Biomedical SciencesInstitute of Tropical MedicineAntwerp 2000Belgium Institute of Experimental Haematology and Transfusion medicine University Hospital BonnBonn 53127Germany mildred Scheel School of Oncology BonnUniversity Hospital BonnMedical FacultyBonn 53127Germany

Radiotherapy induces DNA damage,resulting in cell cycle arrest and activation of cell-intrinsic death ***,the radioresistance of some tumour entities such as malignant melanoma limits its clinical *** innate immune sensing receptor retinoic acid-inducible gene I(RIG-I)is ubiquitously expressed and upon activation triggers an immunogenic form of cell death in a variety of tumour cell types including *** date,the potential of RIG-I ligands to overcome radioresistance of tumour cells has not been ***,we demonstrate that RIG-I activation enhanced the extent and immunogenicity of irradiation-induced tumour cell death in human and murine melanoma cells in vitro and improved survival in the murine B16 melanoma model in *** analysis pointed to a central role for p53,which was confirmed using p53^(-/-)B16 *** vivo,the additional effect of RIG-I in combination with irradiation on tumour growth was absent in mice carrying p53^(-/-)B16 tumours,while the antitumoural response to RIG-I stimulation alone was *** results identify p53 as a pivotal checkpoint that is triggered by RIG-I resulting in enhanced irradiation-induced tumour cell ***,the combined administration of RIG-I ligands and radiotherapy is a promising approach to treating radioresistant tumours with a functional p53 pathway,such as melanoma.

关键词： RIG-I p53 melanoma immunotherapy irradiation radiotherapy radioresistance