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The sexually dimorphic expression of glutamate transporters and their implication in pain after spinal cord injury

Neural Regeneration Research 2025年第11期20卷 3317-3329页

作者： Jennifer m.Colón-mercado Aranza I.Torrado-Tapias Iris K.Salgado Jose m.Santiago Samuel E.Ocasio Rivera Dina P.Bracho-Rincon Luis H.Pagan Rivera Jorge D.miranda department of Physiology University of Puerto RicoMedical Sciences CampusSan JuanPRUSA National Institutes of Health National Human Genome Research InstituteBethesdaMDUSA Universidad Central del Caribe School of MedicineBayamónPRUSA University of Puerto Rico Carolina CampusCarolinaPRUSA Institute of Neurobiology San JuanPRUSA

In addition to the loss of motor function,~60% of patients develop pain after spinal cord *** cellular-molecular mechanisms are not well understood,but the data suggests that plasticity within the rostral,epicenter,and caudal penumbra of the injury site initiates a cellularmolecular interplay that acts as a rewiring mechanism leading to central neuropathic *** can lead to the formation of new connections triggering abnormal sensory *** excitatory glutamate transporters are responsible for the reuptake of extracellular glutamate which makes them a critical target to prevent neuronal hyperexcitability and *** previous studies showed a sexually dimorphic therapeutic window for spinal cord injury after treatment with the selective estrogen receptor modulator *** this study,we investigated the anti-allodynic effects of tamoxifen in male and female rats with spinal cord *** hypothesized that tamoxifen exerts anti-allodynic effects by increasing the expression of glutamate transporters,leading to reduced hyperexcitability of the secondary neuron or by decreasing aberrant *** and female rats received a m.derate contusion to the thoracic spinal cord followed by subcutaneous slow-release treatment of tamoxifen or matrix pellets as a control(placebo).We used von Frey monofilaments and the“up-down method”to evaluate mechanical *** treatment decreased allodynia only in female rats with spinal cord injury revealing a sexdependent *** expression profile of glutamatergic transporters(excitatory amino acid transporter 1/glutamate aspartate transporter and excitatory amino acid transporter 2/glutamate transporter-1)revealed a sexual dimorphism in the rostral,epicenter,and caudal areas of the spinal cord with a pattern of expression primarily on *** rodents showed a significantly higher level of excitatory amino acid transporter-1 expression while male rodents showed increased

关键词： allodynia central neuropathic pain EAAT-1/GLAST EAAT-2/GLT-1 glutamate transporters selective estrogen receptor modulator sexual dimorphism spinal cord injury trauma

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Search for the rare decay B^(0)→J/ψФ

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Chinese Physics C 2021年第4期45卷 47-60页

作者： R.Aaij C.Abellin Beteta T.Ackernley B.Adeva m.Adinolfi H.Afsharnial C.A.Aidala S.Aiola Z.Ajaltouni S.Akar J.Albrecht F.Alessio m.Alexander A.Alfonso Albero Z.Aliouche G.Alkhazov P.Alvarez Cartelle S.Amato Y.Amhis L.An L.Anderlini A.Andreianov m.Andreotti F.Archilli A.Artamonov m.Artuso K.Arzymatov E.Aslanides m.Atzeni B.Audurier S.Bachmann m.Bachmaver J.J.Back S.Baker P.Baladron Rodriguez V.Balagura W.Baldinit J.Baptista Leite R.J.Barlow S.Barsuk W.Barter m.Bartolini F.Baryshnikov J.m.Basels G.Bassi B.Batsukh A.Battig A.Bay m.Becker F.Bedeschis I.Bediaga A.Beiter V.Belavin S.Belin V.Bellee K.Belous I.Belov I.Belyaev G.Bencivenni E.Ben-Haim A.Berezhnoy *** D.Berninghoff H.C.Bernstein C.Bertella E.Bertholet A.Bertolin C.Betancourt F.Betti m.O.Bettler Ia.Bezshyiko S.Bhasin J.Bhom L.Bian m.S.Bieker S.Bifani P.Billoir m.Birch F.C.R.Bishop A.Bizzeti m.Biorn m.P.Blago T.Blake F.Blanc S.Blusk D.Bobulska J.A.Boelhauve O.Boente Garcia T.Boettcher A.Boldyrev A.Bondar N.Bondar S.Borghi m.Borisyak m.Borsato J.T.Borsuk S.A.Bouchiba T.J.V.Bowcock A.Boyer C.Bozzi m.J.Bradley S.Braun A.Brea Rodriguezs m.Brodski J.Brodzicka A.Brossa Gonzalo D.Brundu A.Buonaura C.Burr A.Burschee A.Butkevich J.S.Butter J.Buytaert W.Byczynski S.Cadeddu H.Cai R.Calabrese L.Calefice L.Calero Diaz S.Cali R.Calladine m.Calvi m.Calvo Gomez P.Camargo magalhaes A.Camboni P.Campana D.H.Campora Perez A.F.Campoverde Quezada S.Capelli L.Capriotti A.Carbonel G.Carboni R.Cardinale A.Cardini I.Carli P.Carniti L.Carus K.Carvalho Akiba A.Casais Vidal G.Casse m.Cattaneo G.Cavallero S.Celani J.Cerasoli A.J.Chadwick m.G.Chapman m.Charles Ph.Charpentier G.Chatzikonstantinidis C.A.Chavez Barajas m.Chefdeville C.Chen S.Chen A.Chernov S.-G.Chitic V.Chobanova S.Cholak m.Chrzaszcz A.Chubykin V.Chulikov P.Ciambrone m.F.Cicala X.Cid Vidal G.Ciezarek P.E.L.Clarke m.Clemencic H.V.Cliff J.Closier J.L.Cobbledick V.Coco J.A.B.Coelho J.Cogan E.Cogneras L.Cojocariu P.Collins T.Colombo L.Congedo A.Contu N.Cooke G.Coombs G.Corti C.m.Costa Sobral B.Couturier D.C.Craik J.Crkovska m.Cruz Centro Brasileiro de Pesquisas Fisicas(CBPF) Riode JaneiroBrazil Universidade Federal do Riode Janeiro(UFRJ) Riode JaneiroBrazil Center for High Energy Physics Tsinghua UniversityBeijingChina School of Physics State Key Laboratory of Nuclear Physics and Technology Peking UniversityBeijingChina University of Chinese Academy of Sciences BeijingChina Institute Of High Energy Physics(IHEP) BejingChina Institute of Particle Physics Central China Normal UniversityWuhanHubeiChina Univ.Grenoble Alpes Univ.Savoie Mont BlancCNRSIN2P3-LAPPAnnecyFrance Universite Clermont Auvergne CNRS/IN2P3LPCClermont-FerrandFrance Aix marseille Univ CNRS/IN2P3CPPMMarseilleFrance Universitq Paris-Saclay CNRS/IN2P3IJCLabOrsayFrance LPNHE Sorbonne UniversiteParis Diderot Sorbonne Paris CiteCNRS/IN2P3ParisFrance I.Physikalisches Institut RWTH Aachen UniversityAachenGermany Fakultat Physik Technische Universitat DortmundDortmundGermany max-Planck-Institut fir Kernphysik(mPIK) HeidelbergGermany Physikalisches Institut Ruprecht-Karls-Universitat HeidelbergHeidelbergGermany School of Physics University College DublinDublinIreland INFN Sezione di Bari BariItaly INFN Sezione di Bologna BolognaItaly INFN Sezione di Ferrara FerraraItaly INFN Sezione di Firenze FirenzeItaly INFN Laboratori Nazionali di Frascati Frascatiltaly INFN Sezione di Genova GenovaItaly INFN Sezione di milano Bicocca Milanolaly INFN Sezione di milano MilanoItaly INFN Sezione di Cagliari Monserratoltaly Universita degli Studi di Padova Universita e INFNPadovaPadovaItaly INFN Sezione di Pisa PisaItaly INFN Sezione di Roma Tor Vergata RomaItaly INFN Sezione di Roma La Sapienza RomaItaly Nikhef National Institute for Subatomic Physics AmsterdamNetherlands Nikhef National Institute for Subatomic Physics and VU University Amsterdam AmsterdamNetherlands Henryk Niewodniczanski Institute of Nuclear Physics Polish Academy of Sciences KrakowPoland AGH-University of Science and Technology Faculty of Physics and Applied Computer Science

A search for the rare decay B^(0)→J/ψФis performed using Pp collision data collected with the LHCb dete-ctor at centre-of-mass energies of 7,8 and 13 TeV,corresponding to an integrated luminosity of9 *** significant signal of the decay is observed and an upper limitof 1.1x 10^(-7)at 90%confidence level is set on the branching fraction.

关键词： B physics flavour physics rare decay ω-Фmixing branching fraction

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Considerations for the Conduction and Interpretation of FAIRness Evaluations

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Data Intelligence 2020年第1期2卷 285-292页

作者： Ricardo de miranda Azevedo michel Dumontier Institute of Data Science Maastricht UniversityUniversiteitssingel 60Maastricht 6229 ERThe Netherlands

The FAIR principles were received with broad acceptance in several scientific ***,there is still some degree of uncertainty on how they should be *** self-report questionnaires have been proposed to assess the implementation of the FAIR ***,the FAIRmetrics group released 14,general-purpose maturity for representing ***,these metrics were conducted as open-answer ***,these metrics have been implemented into a software that can automatically harvest metadata from metadata providers and generate a principle-specific FAIRness *** so many different approaches for FAIRness evaluations,we believe that further clarification on their limitations and advantages,as well as on their interpretation and interplay should be considered.

关键词： FAIR metrics FAIR principles FAIR data FAIRness Implementation maturity indicators Scientific community

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Experimental salt cavern in offshore ultra-deep water and well designevaluation for CO_(2) abatement

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International Journal of mining Science and Technology 2019年第5期29卷 641-656页

作者： Alvaro maia da Costa Pedro V.m.Costa Antonio C.O.miranda mariana B.R.Goulart Okhiria D.Udebhulu Nelson F.F.Ebecken Ricardo C.Azevedo Sérgio m.de Eston Giorgio de Tomi Andre B.mendes Julio R.meneghini Kazuo Nishimoto Claudio mueller Sampaio Camila Brandao Alexandre Breda University of Sao Paulo(USP) Sao Paulo 01000-000Brazil m.decom-Technology in Geomechanics and Computing m.deling Rio de Janeiro 27570-000Brazil department of Civil and Environment Engineering University of Brasília(UnB)Brasília 70000-000Brazil COPPE Federal University of Rio de JaneiroRio de Janeiro 27570-000Brazil Technology Division of Shell Shell BrazilRio de Janeiro 27570-000Brazil

This paper presents a proposal for an experimental salt cavern in offshore ultra-deep water for CO2 abatement,including the instrumentation plan and well conceptual design evaluated for carbon capture and storage(CCS)*** studies are based on applied computational mechanics associated with field experimentation that has contributed to the technical feasibility of the underground potash mine at the State of Sergipe in *** knowhow allowed the stability analysis of several salt caverns for brine production at the State of Alagoas in Brazil and to the drilling through stratified thick layers of salt of the pre-salt reservoirs in Santos ***,this knowledge has been applied in the design of onshore and offshore salt caverns opened by dissolution for storage of natural gas and *** geomechanical study,through the application of computational mechanics,of offshore giant salt caverns of 450 m high by 150 m in diameter,shows that one cavern can store about 4 billion Sm3 or 7.2 million tons of *** the construction of the giant cavern,which will be the first gas storage offshore in the world,it has been decided to develop an experimental one,with smaller size,to obtained field *** experimental cavern will allow the calibration of parameters to be used in the structural integrity analysis of the cavern and well for storage of natural gas which is rich in CO2 under high pressure.

关键词： Salt cavern Pre-salt reservoir Geomechanical study Carbon capture and storage

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Renin-angiotensin system in the central nervous system:focus on Huntington’s disease

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Neural Regeneration Research 2023年第10期18卷 2206-2207页

作者： Aline Silva de miranda Antonio Lucio Teixeira Laboratório de Neurobiologia Departamento de MorfologiaInstituto de Ciências BiológicasUniversidade Federal de Minas Gerais(UFMG)Belo HorizonteMGBrasil Laboratório Interdisciplinar de Investigação médica(LIIm) Faculdade de MedicinaUniversidade Federal de Minas Gerais(UFMG)Belo HorizonteMGBrasil Neuropsychiatry Program Department of Psychiatry and Behavioral SciencesMcGovern Medical SchoolUniversity of Texas Health Science Center at HoustonTXUSA Faculdade Santa Casa BH Belo HorizonteMGBrasil

The renin-angiotensin system(RAS)was originally conceived as a circulating hormonal system involved in the regulation of cardiovascular and renal *** the discovery of local RAS components in diverse organs,including t... 详细信息

关键词： Huntington organs angiotensin

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Reinforcement of Hydroxypropylcellulose Films by Cellulose Nanocrystals in the Presence of Surfactants

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Chinese Journal of Polymer Science 2016年第11期34卷 1301-1301,1302-1310页

作者： Noor Rehman maria Inez G.de miranda Simone m.L.Rosa Clara I.D.Bica Instituto de Quimica Universidade Federal do Rio Grande do Sul 91501-970 Porto Alegre/RS Brazil department of Chemistry Shaheed Benazir Bhutto University 18000 Sheringal Dir (Upper) KPK Pakistan Instituto de Ciencias Exatas e Tecnologicas Universidade FEEVALE Campus Ⅱ 93525-075 Novo Hamburgo/RS Brazil

Hydroxypropylcellulose（HPC） films were prepared by casting with cellulose nanocrystals in the presence of anionic surfactant sodium dodecylsulphate（SDS） and cationic surfactant hexadecyltrimethyl ammonium brom.de（CTAB）. The cellulose nanocrystals were isolated from maize straw, a biomass source produced in huge quantities as an agrowaste in Brazil. These bionanocomposite films had good transparency and their surface hydrophilic character was evidenced by static contact angle measurements. Thermogravimetry（TGA） measurement revealed that nanocrystals and surfactants changed the thermal stability of the HPC films. Dynamic mechanical analysis（DmA） showed that the tensile storage and loss moduli of the HPC films increased by increasing the contents of cellulose nanocrystals and surfactants, especially in the case of CTAB. This good reinforcing effect of HPC matrix can be explained as due to electrostatic attractive interactions brought about by the presence of CTAB and the nanocrystals.

关键词： surfactant attractive hydrophilic cellulose reinforcing modulus cationic electrostatic tensile ammonium

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NiTi laser textured implants with improved in vivo osseointegration:An experimental study in rats

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Journal of materials Science & Technology 2022年第19期114卷 120-130页

作者： m.m.Costa A.miranda F.Bartolomeu O.Carvalho S.matos G.miranda F.S.Silva Center for microElectromechanical Systems(CmEmS-Uminho) University of MinhoCampus de AzurémGuimarães 4800-058Portugal Life and Health Sciences Research Institute(ICVS) School of MedicineUniversity of MinhoCampus de GualtarBraga 4710-057Portugal ICVS/3B’s-PT Government Associate Laboratory Braga/GuimarãesPortugal Center for Innovation and Research in Oral Sciences(CIROS) Faculty of MedicineUniversity of CoimbraFMUCPortugal CICECO Aveiro Institute of MaterialsDepartment of Materials and Ceramic EngineeringUniversity of AveiroAveiro 3810-193Portugal

Laser surface texturing is a versatile approach for manufacturing implants with suitable surfaces for *** work explores the use of laser to fabricate NiTi textured implants,testing two dif-ferent groove-based *** performance was evaluated in vivo through implantation in Sprague Dawley rats’femur,being then analyzed after 4 and 12 weeks of ***-out experiments and histological characterization allowed to assess bone-implant bond and osseointegration and to compare the laser textured solutions with non-textured *** showed that,at 4 weeks of implantation,mainly immature woven bone was present whilst at 12 weeks a more mature bone had ***-sidering the largest implantation time(12 weeks),results showed extraction forces considerably higher for textured implants(G2 and G3).moreover,when comparing G2 and G3,it was found that G2(having the highest textured surface area)displayed the maximum extraction force among all groups,with an increase of 212%when compared to non-textured implants(G1).These results prove that the design and manufacturing technology are effective to promote an im-proved bone-implant bond,aiming the development of orthopedic implants.

关键词： NiTi Laser surface texturing In vivo studies Osseointegration Implants

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Tritrichomonas foetus:New structures by high-resolution scanning helium ion microscopy

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BIOCELL 2021年第2期45卷 259-266页

作者： mARLENE BENCHImOL ABIGAIL miranda-mAGALHÃES ANTONIO PEREIRA-NEVES WANdeRLEY de SOUZA Universidade do Grande Rio Duque de Caxias25071-202Rio de Janeiro departamento de microbiologia Laboratório de Biologia Celular de PatógenosFiocruzInstituto Aggeu MagalhãesRecife52060-269Brazil Universidade Federal do Rio de Janeiro Instituto de Biofísica Carlos Chagas FilhoLaboratório de Ultraestrutura Celular Hertha Meyer Instituto Nacional de Ciência e Tecnologia(INBEB)and Centro Nacional de Biologia Estrutural e Bioimagens(CENABIO)Rio de Janeiro21941-902Brazil

Helium ion scanning microscopy(HIm)is a novel high-resolution scanning microscopy technique that uses helium ions instead of electrons to form images of the highest quality and resolution,providing a sub-nanometer resolution sputter uncoated biological ***,we took advantage of HIm to explore the cell surface of Tritrichomonas foetus,a protist parasite of cattle that provokes hard infection and abortion in *** describe thin protrusions,like nanotubes described in other cells,with different sizes(27 nm to 81 nm in thickness)and various lengths(from 73 nm to 2μm),as well bulbous structures either budding from the cell surface or present in the extremities of some *** flagella also presented these thin protrusions and different protein decoration,similar to those previously described using freeze-fracture *** between two cells were also seen,and their role in infection is *** cell surface was also examined and showed several pits indicative of endocytic activity and other types of arrays of *** observations were confirmed using Scanning Electron microscopy(SEm),negative staining,and conventional thin sectioning for observation by transmission electron *** findings provide new and relevant information that may contribute to a better understanding of protozoan biology and its interaction with mammalian cells.

关键词： Nanotubes microvesicles Surface specializations

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Effect of silymarin on biochemical indicators in patients with liver disease: systematic review with meta-analysis

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World Journal of Gastroenterology 2017年第27期23卷 5004-5017页

作者： Camila Ribeiro de Avelar Emile miranda Pereira Priscila Ribas de Farias Costa Rosangela Passos de Jesus Lucivalda Pereira magalhaes de Oliveira Postgraduation Program Federal University of Bahia departament of Science Nutrition Federal University of Bahia

To evaluate the effect of silymarin on the serum levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST) and gamma glutamyl transpeptidase (γGT) in patients with liver diseases. mETHODSA systematic review with meta-analysis of ramdomized and controlled clinical trials was performed, evaluating the effects of sylimarin in patients with hepatic diseases, published by January 31, 2016. Clinical trials were sought on the basis of The Cochrane Central Register of Controlled Trials in the Cochrane Library, Pubmed/medline, Scopus, Web of Science, Lilacs and Clinical Trials. The trials with adult and elderly patients of both sexes, with Liver Diseases who took oral silymarin supplementation, as extract or isolated, as well as Silymarin combined with other nutrients, were included. The trials should provide information about the intervention, such as dosages and detailing of the product used, besides the mean and standard deviation of serum levels of ALT, AST and γGT of the baseline and at the end of the intervention. RESULTSAn amount of 10904 publications were identified. From those, only 17 were included in the systematic review and 6 in the meta-analysis, according to the used selection criteria. In this meta-analysis, the results indicated a reduction of 0.26 IU/mL (95%CI: -0.46-0.07, P = 0.007) at the level of ALT and 0.53 IU/mL (95%CI: -0.74-0.32, P = 0.000) at the serum levels of AST after using the silymarin, both, statistically significant, but with no clinical relevance. There was no significant change in the γGT levels. Subgroup analyzes were also performed for the biochemical markers in relation to the type of intervention, whether silymarin isolated or associated with other nutrients and the time of intervention (whether ≥ 6 mo or mo). Significant differences were not found. The evaluated studies presented a high degree of heterogeneity and low methodological quality in the carried out analysis. CONCLUSIONSilymarin minimally reduced, but w

关键词： Systematic review Liver diseases milk thistle Silymarin meta-analysis

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multiple Redox and Non-Redox Interactions define 2-Cys Peroxiredoxin as a Regulatory Hub in the Chloroplast

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molecular Plant 2009年第6期2卷 1273-1288页

作者： meenakumari muthuramalingam Thorsten Seidel miriam Laxa Susana m. Nunes de miranda Florian Gartner Elke Stroher Andrea Kandlbinder Karl-Josef Dietz Biochemistry and Physiology of Plants W5-134 Bielefeld University 33501 Bielefeld Germany

In plants, the highly abundant 2-cysteine peroxiredoxin （2-CysPrx） is associated with the chloroplast and involved in protecting photosynthesis. This work addresses the multiple interactions of the 2-CysPrx in the chloroplast, which depend on its redox state. Transcript co-regulation analysis showed a strong linkage to the peptidyl-prolyl-cis/trans isomerase Cyclophilin 20-3 （Cyp20-3） and other components of the photosynthetic apparatus. Co-expression in protoplasts and quantification of fluorescence resonance energy transfer （FRET） efficiency in vivo confirmed protein interactions of 2-CysPrx with Cyp20-3 as well as NADPH-dependent thioredoxin reductase C （NTRC）, while thioredoxin x （Trx-x） did not form complexes that could enable FRET. Likewise, changes in FRET of fluorescently labeled 2-CysPrx in vitro and in vivo proved redox dependent dynamics of 2-CysPrx. Addition of Cyp20-3 to an in vitro peroxidase assay with 2-CysPrx had no significant effect on peroxide reduction. Also, in the presence of NTRC, addition of Cyp20-3 did not further enhance peroxide reduction. In addition, 2-CysPrx functioned as chaperone and inhibited aggregation of citrate synthase during heat treatment. This activity was partly inhibited by Cyp20-3. As a new interaction partner of decameric 2-CysPrx, photosystem Ⅱ could be identified after chloroplast fractionation and in pull-down assays after reconstitution. In summary, the data indicate a dynamic function of plant 2-CysPrx as redox sensor, chaperone, and regulator in the chloroplast with diverse functions beyond its role as thiol peroxidase.

关键词： Oxidative and photo-oxidative stress photosynthesis chloroplast biology Arabidopsis cyclophilin peroxiredoxin thioredoxin.

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