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Asian pacific Journal of Reproduction 2024年第5期13卷 219-227页

作者： Vu d Hop An m Cuong Phi T T Anh Nguyen T L Huong Le Hoang Nguyen V Hanh Assisted Reproductive Technology Centre Tam Anh General HospitalHanoiVietnam Institute of Biotechnology Vietnam Academy of Science and TechnologyHanoiVietnam

Objective:To determine the relationship between the early embryo viability assessment(EEVA)and blastocyst morphological parameters and pregnancy ***:This retrospective cohort study was conducted on 291 intracytoplasmic sperm injection cycles including 2522 embryos with indications of prolonging embryo culture to the blastocyst stage in the Genea embryo review incubator,and 511 single vitrified-warmed blastocyst transfer cycles from January 2020 to June *** EEVA system produced an EEVA score from E1(best)to E5(worse)for the potential of blastocyst *** morphology was *** association between the EEVA score and each type of blastocyst morphology,implantation rate,clinical pregnancy,and ongoing pregnancy were assessed using generalized estimating ***:The inner cell mass A(ICm A),trophectoderm A(TE A),blastocoele expansion degree of 3,4,5,6,7 rates were higher with lower the EEVA *** adjusted odd ratio(aOR)(E5 vs E1)was 0.3 for ICm A,0.174 for TE A and 0.210 for BL3,4,5,6,7(all Pd improved embryo *** implantation,clinical pregnancy,and ongoing pregnancy rate were also higher with lower the EEVA *** aOR of E5 vs E1 was 0.245 for implantation,0.185 for clinical pregnancy and 0.200 for ongoing pregnancy rate(Pmorphology,pregnancy outcome and EEVA *** good blastocyst morphology and pregnancy outcomes are higher with lower the EEVA score.

关键词： Automated embryo assessment Blastocyst Early embryo viability assessment Genea embryo review incubator

Influence of previous plane of nutrition on molecular mechanisms regulating the expression of urea and water metabolism related genes in the rumen and kidney of finishing crossbred Angus steers

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Animal Nutrition 2024年第2期17卷 232-243页

作者： Aghata E.moreira da silva Arturo macias Franco Bradley S.Ferguson mozart A.Fonseca College of Agriculture Biotechnology&Natural Resources University of Nevada department of Animal and Range Sciences Clayton Livestock Research Center New Mexico State University

This study aimed to understand how molecular mechanisms controlling water and urea metabolism at the finishing phase can be affected by previous plane of nutrition of crossbred Angus beef steers. Twentyfour(n = 24) animals were randomly distributed into either a moderate(mP) or high plane of nutrition during the background phase for 85 d. Animals were then blocked by their previous plane and were moved onto a 105-d finishing phase in a 2 × 2 factorial arrangement. The forage-finished group received only high-quality alfalfa hay, whereas the grain-fed group received a high grain diet(80% whole corn and20% alfalfa hay). By the end of the finishing phase, animals were harvested and tissue samples from the rumen and kidney were collected. Changes in gene expression of aquaporins(AQP)-2,-3,-4,-7, ATP1A1,ATP1B1, SGK1, CLIC1(kidney and rumen), UT-A1(kidney only) and UT-B(rumen only), were assayed via real-time q PCR; 18S r RNA was used as an endogenous control. One-way ANOVA followed by Tukey's post hoc analysis was conducted. When animals were from mP, forage-finishing increased the relative abundance of AQP3(P ≤ 0.05), AQP7(P ≤ 0.05), ATP1B1(P ≤ 0.05), and SGK1(P ≤ 0.05) in the kidney when compared to grain-fed animals. In the rumen, for the mP group, AQP7 was differentially expressed in both treatments at the finishing phase(P ≤ 0.01), with forage-finished steers having the highest expression of AQP7. For the mP group, UT-B had a tendency of presenting a higher expression on grainfed animals(P = 0.075). Overall, these results suggest that previous plane can impact expression of genes associated with water and urea metabolism during the finishing phase, namely AQP3, AQP7, ATP1B1, and SGK1 in the kidney, and AQP7 and UT-B in the rumen. The greatest impact observed on gene expression changes of investigated genes at the finishing phase was reflective of animals backgrounded on the moderate previous plane.

关键词： Gene expression Aquaporin Sodium channel Urea transporter

In vivo femtosecond laser nanosurgery of the cell wall enabling patch-clamp measurements on filamentous fungi

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microsystems & Nanoengineering 2024年第2期10卷 251-267页

作者： Tanja Pajić Katarina Stevanović Nataša V.Todorović Aleksandar J.Krmpot miroslav Živić Svetlana Savić-Šević Steva m.Lević marina Stanić dejan Pantelić Brana Jelenković mihailo d.Rabasović Institute of Physiology and Biochemistry“Ivan djaja” Faculty of BiologyUniversity of BelgradeStudentski trg 1611158BelgradeSerbia Institute for Biological Research“Siniša Stanković” University of BelgradeNational Institute of the Republic of SerbiaBulevar Despota Stefana 14211000BelgradeSerbia Institute of Physics Belgrade University of BelgradeNational Institute of the Republic of SerbiaPregrevica 11811080BelgradeSerbia University of Belgrade Faculty of AgricultureNemanjina Street 611080BelgradeSerbia Institute for multidisciplinary Research University of BelgradeKneza Višeslava 111030BelgradeSerbia

Studying the membrane physiology of filamentous fungi is key to understanding their interactions with the environment and crucial for developing new therapeutic strategies for disease-causing ***,their plasma membrane has been inaccessible for a micron-sized patch-clamp pipette for pA current recordings due to the rigid chitinous cell ***,we report the first femtosecond IR laser nanosurgery of the cell wall of the filamentous fungi,which enabled patch-clamp measurements on protoplasts released from hyphae.A reproducible and highly precise(diffraction-limited,submicron resolution)method for obtaining viable released protoplasts was *** release from the nanosurgery-generated incisions in the cell wall was achieved from different regions of the *** plasma membrane of the obtained protoplasts formed tight and high-resistance(GΩ)contacts with the recording *** entire nanosurgical procedure followed by the patch-clamp technique could be completed in less than 1 *** to previous studies using heterologously expressed channels,this technique provides the opportunity to identify new ionic currents and to study the properties of the ion channels in the protoplasts of filamentous fungi in their native environment.

关键词： patch recording laser

Post-operative protein supplementation following orthopaedic surgery: A systematic review

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Sports medicine and Health Science 2024年第1期6卷 16-24页

作者： Andrew George Brendan m.Holderread Bradley S.Lambert Joshua d.Harris Patrick C.mcCulloch Houston methodist Orthopedics and Sports medicine 6445 Main Street Suite 2300HoustonTX77030USA Houston methodist Orthopedic Biomechanics Research Laboratory 6670 Bertner AveHoustonTX77030USA

decreased mechanical loading after orthopaedic surgery predisposes patients to develop muscle *** purpose of this review was to assess whether the evidence supports oral protein supplementation can help decrease postoperative muscle atrophy and/or improve patient outcomes following orthopaedic surgery.A systematic review was conducted according to the Preferred Reporting Items for Systematic Reviews and metaAnalysis(PRISmA).Pubmed(mEdLINE),Embase,Scopus,and Web of Science were searched for randomized controlled trials that assessed protein or amino acid supplementation in patients undergoing orthopaedic *** investigators independently conducted the search using relevant Boolean *** outcomes included functional or physiologic measures of muscle atrophy or *** studies including 611 patients(224 males,387 females)were *** studies evaluated protein supplementation after ACL reconstruction(ACLR),3 after total hip arthroplasty(THA),5 after total knee arthroplasty(TKA),and 3 after surgical treatment of hip *** supplementation showed beneficial effects across all types of *** primary benefit was a decrease in muscle atrophy compared to placebo as measured by muscle cross sectional *** authors also demonstrated improved functional measures and quicker achievement of rehabilitation *** supplementation has beneficial effects on mitigating muscle atrophy in the postoperative period following ACLR,THA,TKA,and surgical treatment of hip *** effects often correlate with improved functional measures and quicker achievement of rehabilitation *** research is needed to evaluate long-term effects of protein supplementation and to establish standardized population-specific regimens that maximize treatment efficacy in the postoperative period.

关键词： Orthopaedic surgery Protein supplementation Systematic review muscle atrophy

development of an ultrathin liquid sheet target for laser ion acceleration at high repetition rates in the kilohertz range

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High Power Laser Science and Engineering 2024年第3期12卷 134-145页

作者： m.Füle A.P.Kovács T.Gilinger m.Karnok P.Gaál S.Figul G.marowsky K.Osvay National Laser-Initiated Transmutation Laboratory University of SzegedSzegedHungary department of Experimental Physics University of SzegedSzegedHungary ELI ALPS ELI-HU Non-Profit Ltd.SzegedHungary department of Optics and Quantum Electronics University of SzegedSzegedHungary Advanced microfluidic Systems GmbH GöttingenGermany

A colliding microjet liquid sheet target system was developed and tested for pairs of round nozzles of 10,11 and 18μm in *** sheet's position stability was found to be better than a few *** interaction with 50 mJ laser pulses,the 18μm jet has a resonance amplitude of 16μm at a repetition rate of 33 Hz,while towards 100 Hz it converges to 10μm for all nozzles.A white-light interferometric system was developed to measure the liquid sheet thickness in the target chamber both in air and in vacuum,with a measurement range of 182 nm±1μm and an accuracy of±3%.The overall shape and 3d shape of the sheet follow the Hasson±Peck model in *** vacuum versus air,the sheet gradually loses 10%of its thickness,so the thinnest sheet achieved was below 200 nm at a vacuum level of 10±4mbar,and remained stable for several hours of operation.

关键词： diagnostics laser ion acceleration nanometric liquid sheet targets vacuum test

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Role of marine natural products in the development of antiviral agents against SARS‑CoV‑2:potential and prospects

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marine Life Science & Technology 2024年第2期6卷 280-297页

作者： d.P.Nagahawatta N.m.Liyanage Thilina U.Jayawardena H.H.A.C.K.Jayawardhana Seong‑Hun Jeong Hyung‑Jun Kwon You‑Jin Jeon department of marine Life Sciences Jeju National UniversityJeju 690-756Republic of Korea department of Chemistry Biochemistry and PhysicsUniversitédu QuébecàTrois-RivièresTrois-RivièresQC G8Z 4M3Canada marine Science Institute Jeju National UniversityJeju 63333Republic of Korea Functional Biomaterial Research Center Korea Research Institute of Bioscience and BiotechnologyJeongeup-siRepublic of Korea

A novel coronavirus,known as severe acute respiratory syndrome coronavirus 2(SARS-CoV-2),has surfaced and caused global concern owing to its ***-CoV-2 is the causative agent of coronavirus disease 2019;however,it was only discovered at the end of the year and was considered a pandemic by the World Health ***,the develop-ment of novel potent inhibitors against SARS-CoV-2 and future outbreaks is urgently *** naturally occurring bioactive substances have been studied in the clinical setting for diverse *** intricate infection and replication mechanism of SARS-CoV-2 offers diverse therapeutic drug targets for developing antiviral medicines by employing natural products that are safer than synthetic *** natural products(mNPs)have received increased attention in the development of novel drugs owing to their high diversity and ***,this review article investigates the infection and replication mechanisms,including the function of the SARS-CoV-2 genome and ***,we highlighted anti-SARS-CoV-2 therapeutic intervention efforts utilizing mNPs and predicted SARS-CoV-2 inhibitor design.

关键词： SARS-CoV-2 marine natural products drug targets Inhibitors

Vanillylacetone attenuates cadmium chloride-induced hippocampal damage and memory loss through upregulation of nuclear factor erythroid 2-related factor 2 gene and protein expression

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Neural Regeneration Research 2024年第12期19卷 2750-2759页

作者： Fahaid H.A.L-Hashem Salah O.Bashir Amal F.dawood moutasem S.Aboonq Ismaeel Bin-Jaliah Abdulaiziz m.Al-Garni mohamed d.morsy department of Physiology College of MedicineKing Khalid UniversityAbhaSaudi Arabia department of Basic medical Sciences College of MedicinePrincess Nourah bint Abdulrahman UniversityP.O.Box 84428Riyadh 11671Saudi Arabia department of Physiology College of MedicineTaibah UniversityAl-Madinah Al-MunawwarahSaudi Arabia department of medicine College of MedicineKing Khalid UniversityAbhaSaudi Arabia

memory loss and dementia are major public health concerns with a substantial economic *** stress has been shown to play a crucial role in the pathophysiology of hippocampal damage-induced memory *** investigate whether the antioxidant and anti-inflammatory compound vanillyla cetone(zingerone) can protect against hippocampal damage and memory loss induced by cadmium chloride(CdCl_(2)) administration in rats,we explo red the potential involvement of the nuclear factor erythroid 2-related factor 2(Nrf2) signaling pathway,which is known to modulate oxidative stress and *** healt hy male Wistar rats were divided into five groups:vehicle-treated(control),vanillylacetone,CdCl_(2),vanillylacetone+ CdCl_(2),vanillylacetone+ CdCl_(2)+ brusatol(a selective pharmacological N rf2inhibitor) *** effectively attenuated CdCl_(2)-induced damage in the dental gyrus of the hippocampus and improved the memory function assessed by the morris Water maze ***,vanillylacetone markedly decreased the hippocampal tissue levels of inflammatory biomarkers(interleukin-6,tumor necrosis factor-α,intracellular cell adhesive molecules) and apoptosis biomarkers(Bax and cleaved caspase-3).The control and CdCl_(2)-treated groups treated with va nillylacetone showed reduced generation of reactive oxygen species,decreased malondialdehyde levels,and increased superoxide dismutase and glutathione activities,along with significant elevation of nuclear Nrf2 mRNA and protein expression in hippocampal *** the protective effects of vanillylacetone we re substantially blocked by the co-administration of brusatol(a selective N rf2 inhibitor).Va nillylacetone mitigated hippocampal damage and memory loss induced by CdCl_(2),at least in part, by activating the nuclear transcription factor ***,vanillylacetone exerted its potent antioxidant and antiinflammatory actions.

关键词： hippocampus neuroprotective Nrf2 gene oxidative stress vanillylacetone

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Rotating effects on the photoionization cross-section of a 2d quantum ring

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Communications in Theoretical Physics 2024年第10期76卷 153-159页

作者： Carlos magno O Pereira Frankbelson dos S Azevedo Luís Fernando C Pereira Edilberto O silva departamento de Física Universidade Federal do Maranh?o

In this article, we investigate the nonrelativistic quantum motion of a charged particle within a rotating frame, taking into account the Aharonov–Bohm(AB) effect and a uniform magnetic field. Our analysis entails the derivation of the equation of motion and the corresponding radial equation to describe the system. Solving the resulting radial equation enables us to determine the eigenvalues and eigenfunctions, providing a clear expression for the energy levels. Furthermore,our numerical analysis highlights the substantial influence of rotation on both energy levels and optical properties. Specifically, we evaluate the photoionization cross-section with and without the effects of rotation. To elucidate the impact of rotation on the photoionization process of the system, we present graphics that offer an appealing visualization of the intrinsic nature of the physics involved.

关键词： Aharonov-Bohm effect photoionization cross-section quantum ring rotating effect

Integrated Single-cell multiomic Analysis of HIV Latency Reversal Reveals Novel Regulators of Viral Reactivation

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Genomics, Proteomics & Bioinformatics 2024年第1期22卷 67-88页

作者： manickam Ashokkumar Wenwen mei Jackson J.Peterson Yuriko Harigaya david m.murdoch david m.margolis Caleb Kornfein Alex Oesterling Zhicheng Guo Cynthia d.Rudin Yuchao Jiang Edward P.Browne department of medicine University of North Carolina at Chapel HillChapel HillNC 27599USA HIV Cure Center University of North Carolina at Chapel HillChapel HillNC 27599USA department of Biostatistics University of North Carolina at Chapel HillChapel HillNC 27599USA department of microbiology and Immunology University of North Carolina at Chapel HillChapel HillNC 27599USA department of Genetics University of North Carolina at Chapel HillChapel HillNC 27599USA department of medicine Duke UniversityDurhamNC 27708USA department of Computer Science Duke UniversityDurhamNC 27708USA department of Statistics Texas A&M UniversityCollege StationTX 77843USA department of Biology Texas A&M UniversityCollege StationTX 77843USA department of Biomedical Engineering Texas A&M UniversityCollege StationTX 77843USA

despite the success of antiretroviral therapy,human immunodeficiency virus(HIV)cannot be cured because of a reservoir of latently infected cells that evades *** understand the mechanisms of HIV latency,we employed an integrated single-cell RNA sequencing(scRNA-seq)and single-cell assay for transposase-accessible chromatin with sequencing(scATAC-seq)approach to simultaneously profile the transcriptomic and epigenomic characteristics of~125,000 latently infected primary Cd4^(+)T cells after reactivation using three different latency reversing *** expressed genes and differentially accessible motifs were used to examine transcriptional pathways and transcription factor(TF)activities across the cell *** identified cellular transcripts and TFs whose expression/activity was correlated with viral reactivation and demonstrated that a machine learning model trained on these data was 75%-79%accurate at predicting viral ***,we validated the role of two candidate HIV-regulating factors,FOXP1 and GATA3,in viral *** data demonstrate the power of integrated multimodal single-cell analysis to uncover novel relationships between host cell factors and HIV latency.

关键词： HIV latency reversal Primary Cd4þT cell Single-cell multiomics HIV-regulating factor machine learning

Extracellular vesicles-coupled miRNAs from oviduct and uterus modulate signaling pathways related to lipid metabolism and bovine early embryo development

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Journal of Animal Science and Biotechnology 2024年第4期15卷 1528-1549页

作者： Rosane mazzarella Karina Canon‑Beltran Yulia N.Cajas meriem Hamdi Encina m.Gonzalez Juliano C.da Silveira Claudia L.V.Leal d.Rizos department of Animal Reproduction INIA-CSICMadridSpain department of Biochemistry and molecular Biology Veterinary FacultyComplutense University of Madrid(UCM)MadridSpain department Agrarian Production Technical University of MadridUPMMadridSpain departamento de Ciencias Biologicas Universidad Tecnica Particular de LojaUTPLLojaEcuador department of Anatomy and Embryology FV-UCMMadridSpain department of Veterinary medicine FZEA-USPPirassunungaBrazil

Background Extracellular vesicles(EVs) present in oviductal(OF) and uterine fluid(UF) have been shown to enhance bovine embryo quality during in vitro culture by reducing lipid contents and modulating lipid metabolism-related genes(LmGs), while also influencing cell proliferation, suggesting their involvement on the regulation of different biological pathways. The regulation of signaling pathways related to cell differentiation, proliferation, and metabo-lism is crucial for early embryo development and can determine the success or failure of the pregnancy. Bioactive molecules within EVs in maternal reproductive fluids, such as micro RNAs(miRNAs), may contribute to this regulatory process as they modulate gene expression through post-transcriptional *** This study evaluated miRNA cargo in OF-EVs from the early luteal phase and UF-EVs from the mid-luteal phase, coinciding with embryo transit within oviduct and uterus in vivo, and its possible influence on LmGs and sign-aling pathways crucial for early embryo development. A total of 333 miRNAs were detected, with 11 exclusive to OF, 59 to UF, and 263 were common between both groups. From the 20 differentially expressed miRNAs, 19 up-regulated in UF-EVs(bta-miR-134, bta-miR-151-3p, bta-miR-155, bta-miR-188, bta-miR-181b, bta-miR-181d, bta-miR-224, bta-miR-23b-3p, bta-miR-24-3p, bta-miR-27a-3p, bta-miR-29a, bta-miR-324, bta-miR-326, bta-miR-345-3p, bta-miR-410, bta-miR-652, bta-miR-677, bta-miR-873 and bta-miR-708) and one(bta-miR-148b) in OF-EVs. These miRNAs were predicted to modulate several pathways such as Wnt, Hippo, mAPK, and lipid metabolism and degradation. differ-ences in miRNAs found in OF-EVs from the early luteal phase and UF-EVs from mid-luteal phase may reflect differ-ent environments to meet the changing needs of the embryo. Additionally, miRNAs may be involved, particularly in the uterus, in the regulation of embryo lipid metabolism, immune system, and *** Our study su

关键词： Embryo-maternal interaction Epigenetic Exosomes mammalian Preimplantation Reproductive fluids

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