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Sublethal Effects of the Formamidine Amitraz on Honeybees Gut Lipids, Following in vivo Injections

Biomedical and Environmental Sciences 1991年第4期4卷 376-383页

作者： K.m'DIAYE m.bounias Laboratory of Biochemistry INRA-Avignon B.P.91 F 84143 Montfavet Cédex FranceLaboratory of Biochemistry INRA-Avignon B.P.91 F 84143 Montfavet Cédex France

The time-course and dose-related action of amitraz (AmZ) on gut lipids of worker honeybees were examined over 3 hours following in vivo injections of 0.25, 0.5, 1 and 2 nmols of pesticide per bee. Significant decreases were observed at 30-45 min with 0.25 nmols per bee, for phospholipids, fatty acids, steroids and triacylglycerols. However increases were observed either later or with higher doses. The decreasing action observed with 0.25 nmol AmZ per bee was inhibited by simultaneous injections of the a antagonist phentolamine (from 0.25 to 2.0 nmols per bee). The toxicity of AmZ to honeybees thus likely involves the mobilization of lipids from the gut, via action of this formamidine pesticide on a-adrenoceptors.

关键词： Sublethal Effects of the Formamidine Amitraz on Honeybees Gut Lipids Following in vivo Injections

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The Formamidine Amitraz as a Hyperglycemic α-Agonist in Worker Honeybees(Apis mellifera mellifera L.)in Vivo

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Biomedical and Environmental Sciences 1989年第2期2卷 106-114页

作者： P.CASCINO m.NECTOUX G.GUIRAUD m.bounias Laboratory of Biochemistry INRA-AvignonB.P.91F84140 MontfavetFrance

Intra-abdominal injection of amitraz(0.25 nmol per honeybee,i.e.,approx 2.3 nmol/g)to emerging worker bees,in vivo,led to a significant hypertrehalosemia(300-400%)followed by a hyperglucosemia(≈600%).maxima were reached at 0.5 and 2h,respectively.A strong negative correlation between glucosemia and trehalosemia appeared after injection of pure phentolamine (1 nmol per bee),suggesting stimulation of trehalase *** administration of the α-blocker at≥0.25 nmol per individual suppressed the hyperglycemic response of amitraz. The formamidine pesticide thus likely acts on the honeybee α-aminergic system.1989 Academic Press,Inc.

关键词： in Vivo Agonist in Worker Honeybees Apis mellifera mellifera L The Formamidine Amitraz as a Hyperglycemic

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Time and Dose-related Effects of the Pyrethroid Fluvalinate on Haemolymph Carbohydrates and Gut Lipids of Honeybees,Following in vivo Injection of very Low Doses

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Biomedical and Environmental Sciences 1993年第2期6卷 145-153页

作者： K.m'DIAYE m.bounias University University of AvignonBiomathematics & Toxicology23 rue du 58eR.I.F 84000 AvignonFranceof AvignonBiomathematics & Toxicology23 rue du 58eR.I.F 84000 AvignonFrance

The injection to emerging adult workerbees with fluvalinate doses ranging from 1 femtomol to 1 nanomol per individual resulted in a reduction of haemolymph carbohydrate concentrations, particularly at the lowest dose 1 hour after injections. At the same time, a large increase was observed for triacylglycerols and to a much lesser extent for steroids and phospholipids with 0.1 picomol per bee. By contrast, fatty acids, steroids and triacylglycerols exhibited a depress at the higher dose. most responses were thus biphasic, showing that much attention should be paid to the effects of very low doses of pesticide.

关键词： Time and Dose-related Effects of the Pyrethroid Fluvalinate on Haemolymph Carbohydrates and Gut Lipids of Honeybees,Following in vivo Injection of very Low Doses very

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