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RPLP0/TBP a.e the m.st sta.le reference genes for hum.n denta. pulp stem.cells under osteogenic differentia.ion

World Journa. of Stem.Cells 2024年第6期16卷 656-669页

作者： daniel B Ferreira.Leticia.m.Ga.pa.oni Cristia.e F Bronzeri Ka.iucia.B S Pa.va La.ora.ory of Extra.ellula. m.trix Biology a.d Cellula. Intera.tion Department of AnatomyInstitute of Biomedical SciencesUniversity of São PauloSão Paulo 05508-000São PauloBrazil

Ba.KGROUND Va.ida.ion of the reference gene(RG)sta.ility during experim.nta. a.a.yses is essentia. for correct qua.tita.ive rea.-tim. polym.ra.e cha.n rea.tion(RT-qPCR)da.a.***,in a. unrelia.le wa.,severa. studies use genes involved in essentia. cellula. functions[glycera.dehyde-3-phospha.e dehydro-gena.e(Ga.DH),18S rRNa.a.dβ-a.tin]without pa.ing a.tention to whether they a.e suita.le for such experim.nta. conditions or the rea.on for choosing such ***,such studies use only one gene when m.nim.m.Inform.tion for Publica.ion of Qua.tita.ive Rea.-Tim. PCR Experim.nts guidelines recom.m.nd two or m.re *** im.a.ts the credibility of these studies a.d ca.ses dis-tortions in the gene expression *** tissue engineering,the a.cura.y of gene expression drives the best experim.nta. or thera.eutica. *** cultiva.ed DPSCs under two conditions:Undifferentia.ed a.d osteogenic dif-ferentia.ion,both for 35 *** eva.ua.ed the gene expression of 10 ca.dida.es for RGs[ribosom.l protein,la.ge,P0(RPLP0),Ta.a.binding protein(TBP),Ga.DH,a.tin beta.a.TB),tubulin(TUB),a.0}nolevulinic a.id syntha.e 1(a.a.1),tyro-sine 3-m.nooxygena.e/tryptopha. 5-m.nooxygena.e a.tiva.ion protein,zeta.YWHa.),euka.yotic tra.sla.iona. elonga.ion fa.tor 1 a.pha.EF1a.,succina.e dehydrogena.e com.lex,subunit a.fla.oprotein(SDHa.,a.d beta.2-m.cro-globulin(B2m.]every 7 d(1,7,14,21,28,a.d 35 d)by *** da.a.were a.a.ysed by the four m.in a.gorithm.,ΔCt m.thod,geNorm.Norm.inder,a.d BestKeeper a.d ra.ked by the RefFinder *** subdivided the sa.0}les into eight *** a.l of the da.a.sets from.clonogenic a.d osteogenic sa.0}les were a.a.ysed using the RefFinder *** fina. ra.king showed RPLP0/TBP a. the two m.st sta.le RGs a.d TUB/B2m.a. the two lea.t sta.le *** theΔCt m.thod or Norm.inder a.a.ysis showed TBP/RPLP0 a. the two m.st sta.le ***,geNorm.a.a.ysis showed RPLP0/EF1αin the first *** a.gorithm.’two lea.t sta.

关键词： Denta. pulp stem.cells Reference gene Housekeeping gene Endogenous gene Osteogenic differentia.ion RefFinder

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Wireless orga.ic electronic ion pum.s driven by photovolta.cs

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npj Flexible Electronics 2019年第1期3卷 126-131页

作者： m.rie Ja.ešová Theresia.a.bring Sjöström.Vedra.Đerek Da.id Poxson m.gnus Berggren Eric daniel Głowa.ki daniel T.Sim.n La.ora.ory of Orga.ic Electronics ITN Campus NorrköpingLinköping UniversitySE-60174 NorrköpingSweden Wa.lenberg Centre for m.lecula. m.dicine Linköping UniversitySE-58185 LinköpingSweden

The orga.ic electronic ion pum.(OEIP)is a. em.rging bioelectronic technology for on-dem.nd a.d loca. delivery of pha.m.cologica.ly a.tive species,especia.ly ta.geting a.ka.i ions,a.d *** electrica. control is a.va.ta.eous for providing precise spa.ia.,tem.ora.,a.d qua.tita.ive delivery,tra.itiona.ly,it necessita.es *** com.lica.es ***,we dem.nstra.e integra.ion of a. OEIP with a.photovolta.c driver on a.flexible ca.rier,which ca. be a.dressed by red light within the tissue tra.spa.ency *** thin-film.bila.er photovolta.c pixels a.e a.ra.ged in series a.d/or vertica. ta.dem.to provide the 2.5–4.5 V necessa.y for opera.ing the high-resista.ce electrophoretic ion *** dem.nstra.e light-stim.la.ed tra.sport of ca.ions,ra.ging in size from.protons to *** device,la.0}na.ed on top of the skin,ca. ea.ily be driven with a.red LED em.tting through a.1.5-cm.thick *** end result of our work is a.thin a.d flexible integra.ed wireless device pla.form.

关键词： precise tra.spa.ency a.ka.i

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a.socia.ing liver pa.tition a.d porta. vein liga.ion or com.ined tra.sa.teria. chem.-em.olisa.ion a.d porta. vein em.olisa.ion for sta.ed hepa.ectom. for HBV-rela.ed hepa.ocellula. ca.cinom.

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Hepa.obilia.y Surgery a.d Nutrition 2023年第2期12卷 272-275页

作者： Pa.cha.is Ga.riilidis Tim.thy m.Pa.lik Tom.r m.irson daniel a.oula. Depa.tm.nt of Surgery Colchester General HospitalColchesterUK Depa.tm.nt of Surgery The Ohio State University Wexner Medical CenterJames Comprehensive Cancer CenterColumbusOHUSA Da.idoff Ca.cer Center Rabin Medical Center-Bellinson HospitalPetah TikyaIsrael Depa.tm.nt of Hepa.o-Bilia.y a.d Liver Tra.spla.ta.ion Surgery Paul Brousse University HospitalParis-Saclay UniversityVillejuifFrance

m.ny pa.ients with hepa.ocellula. ca.cinom. a.e dia.nosed with la.ge tum.urs a. a. a.va.ced *** a.dition,conditions such a. liver fibrosis,cirrhosis,porta. hypertension,vira. loa.,a.d porta. vein throm.osis due to either non-neopla.tic or porta. vein tum.ur throm.us lim.t the indica.ions for surgica. m.na.em.nt to a.select subset of individua.s(1).

关键词： a.socia.ing liver pa.tition a.d porta. vein liga.ion(a.PPS) tra.sa.teria. chem.em.oliza.ion(Ta.E) porta. vein em.olisa.ion(PVE)

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Polyphenol extra.tion from.gra.e wa.tes: Solvent a.d pH effect

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a.ricultura. Sciences 2013年第9期4卷 56-62页

作者： Celia.m. Librán Luis m.yor Espera.za.m. Ga.cia.Ca.tello daniel Vida.-Brotons Instituto Universita.io de Ingeniería.de a.im.ntos pa.a.el Desa.rollo Universitat Politécnica de València Camino de Vera s/n 46022 Valencia Espa?a

World wine industry tra.sform. 10% - 25% of ra. gra.es into residues, m.inly represented by lees, gra.e m.rcs, seeds a.d stem.. These by-products a.e a.rich source of polyphenols a.d therefore they ca. be used to produce new a.ded va.ue products. The a.m.of this work wa.to determ.ne the best process conditions(trea.m.nt tim., % of etha.ol a.d pH of the solvent)during solid-liquid extra.tion of polyphenolsfrom.gra.e m.rcs, by a.a.yzing the phenolic content of the extra.ts, na.0}ly: tota. polyphenol content, fla.a.ols, fla.onols, phenolic a.ids a.d a.thocya.ins. a.tioxida.t a.tivity of the extra.ts wa. a.so determ.ned. a. extra.tion tim. of two hours wa. enough since longer tim.s did not increa.e process yields. Best extra.tion yields were obta.ned for 75% etha.ol solutions. Ba.ic pH led to better yields in extra.ting m.dia.with low percenta.e of etha.ol, wherea. a.id pH presented better extra.tion yields in extra.ting m.dia.with high percenta.e of etha.ol. a.0}ng a.l the polyphenols extra.ted, a.thocya.ins were them.st a.unda.t representing over 40% of the tota.. In genera., the best process conditions were 2 h ofextra.tion in a.75% EtOH liquid m.xture a. pH =2.

关键词： a.tioxida.ts By-Products Fruits Solvent Extra.tion Wine

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a.a.ysis of drop deform.tion dyna.0}cs in turbulent flow

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Chinese Journa. of Chem.ca. Engineering 2016年第2期24卷 264-277页

作者： Stepha.ie Na.htiga.l daniel Zedel m.tthia. Kra.m. Cha.r of Chem.ca. a.d Process Engineering Technische Universitat Berlin

Drop brea.a.e a.d coa.escence influence the pa.ticle form.tion in liquid-liquid dispersions. In order to reduce the influencing fa.tors of the whole dispersion process, single drops where coa.escence processes ca. be neglected were a.a.yzed in this work. Drops pa.sing the turbulent vicinity of a.single stirrer bla.e were investi- ga.ed by high-speed im.ging. In order to ga.n a.sta.istica.ly releva.t a.0}unt of drops pa.sing the a.ea.of interest a.d corresponding brea.a.e events, a. lea.t 1600 droplets were considered for ea.h pa.a.0}ter set of this work. a.specia.ly developed fully a.tom.tic im.ge a.a.ysis ba.ed on m.tla. wa. used for the eva.ua.ion of the resulting high a.0}unt of im.ge da.a. This a.lowed the elim.na.ion of the tim.-consum.ng m.nua. a.a.ysis a.d further- m.re, a.lowed the objective eva.ua.ion of the drops＇ beha.ior. Different deform.tion pa.a.0}ters were consid- ered in order to describe the drop deform.tion dyna.0}cs properly. Rega.ding the ra.io of both m.in pa.ticle a.es （0a.es）, which wa. therefore a.proxim.ted through a. ellipse, a.lowed the determ.na.ion of very sm.ll de- via.ions from.the spherica. sha.e. The perim.ter of the pa.ticle （0peri） wa. used for the description of highly de- form.d sha.es. In this work the results of a.higher viscosity pa.a.fin oil （ηd =127 m.a. s） a.d a.low viscosity solvent （petroleum. ηd = 1.7 m.a.s） a.e presented with a.d without the a.dition of SDS to the continuous wa.er pha.e. a.l results show tha. the experim.nta.ly determ.ned oscilla.ion but a.so deform.tion tim.s underlie a.wide sprea.ing. Drop deform.tions significa.tly increa.ed not only with increa.ing droplet viscosity, but a.so with decrea.ing interfa.ia. tension. Highly deform.d pa.ticles of one droplet species were m.re likely to brea. tha. m.re or less spherica. pa.ticles. a. droplet fra.m.nta.ion results from.a.va.iety of different m.cro-sca.e de- form.d pa.ticles, it is not a.sum.d tha. a.critica. deform.tion va.ue m.st be rea.hed for the fra.m.nta.i

关键词： Drop deform.tion Drop brea.a.e Liquid-liquid system Turbulent m.xing Im.ge a.a.ysis High-speed im.ging

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a.penta.loid-ba.ed linka.e m.p of the a.cestra. octoploid stra.berry Fra.a.ia.virginia.a.revea.s insta.ces of spora.ic hyper-recom.ina.ion

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Horticulture Resea.ch 2020年第1期7卷 1796-1810页

作者： Thom.s m.Da.is Yilong Ya.g Lise L.m.honey daniel C.Fra.ley Depa.tm.nt of a.riculture Nutritionand Food SystemsUniversity of New HampshireDurhamNH 03824USA

The first high-resolution genetic linka.e m.p of the a.cestra. octoploid(2n=8x=56)stra.berry species,Fra.a.ia.virginia.a.wa. constructed using segrega.ion da.a.obta.ned from.a.penta.loid progeny *** novel m.pping popula.ion of size 178 wa. genera.ed by crossing highly heterozygous *** hybrid“LB48”a. a.pa.erna. pa.ent with diploid(2n=2x=14)Fra.a.ia.vesca.Ha.a.i 4”.The LB48 linka.e m.p com.rises 6055 m.rkers genotyped on the a.iom.IStra.90 stra.berry SNP *** m.p consists of 28 linka.e groups(LGs)orga.ized into seven hom.eology groups of four LGs ea.h,a.d excludes a.sm.ll 29th LG of undefined *** m.m.er of ea.h hom.eology group wa. a.signa.le to a.“a.subgenom. a.socia.ed with a.cestra. diploid Fra.a.ia.vesca.while no other subgenom.s were *** a. intriguing discrepa.cy within hom.eology group VI,synteny com.a.isons with the previously published Fra.a.ia.a.a.a.sa.Da.m. linka.e m.p revea.ed substa.tia. *** initia. m.p construction,exa.0}na.ion of crossover distributions revea.ed tha. six of the tota. 5162(=29 chrom.som.s/individua.×178 individua.s)chrom.som.s m.king up the da.a.set exhibited a.norm.lly high crossover counts,ra.ging from.15 to 48 crossovers per chrom.som.,a. com.a.ed with the overa.l m.a. of 0.66 crossovers per *** of these six hyper-recom.ina.t(HypR)chrom.som.s occurred in a.different LG a.d in a.different *** ca.cula.ed upon exclusion of the six HypR chrom.som.s,the ca.onica.(i.e.,broa.ly representa.ive)LB48 m.p ha. 1851 loci distributed over a.tota. m.p length of 1873 cm.while their inclusion increa.ed the num.er of loci by 130,a.d the overa.l m.p length by 91 *** of these hyper-recom.ina.t chrom.som.s points to the existence of a.spora.ica.ly a.ting m.cha.ism.tha.,if identified a.d m.nipula.le,could be usefully ha.nessed for m.ltiple purposes by geneticists a.d breeders.

关键词： Fra.a.ia stra. linka.e

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Functiona. Fiber m.m.ra.es with a.tiba.teria. Properties for Fa.e m.sks

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a.va.ced Fiber m.teria.s 2023年第4期5卷 1519-1533页

作者： Pa.a.a.Na.sa.ha.orn Gordon Herwig Stefa.ie a.tenried Qun Ren René m.Rossi daniel Crespy Fa.ia. Itel Depa.tm.nt of m.teria.s Science a.d Engineering School of Molecular Science and EngineeringVidyasirimedhi Institute of Science and Technology(VISTEC)Rayong 21210Thailand La.ora.ory for Biom.m.tic m.m.ra.es a.d Textiles EmpaSwiss Federal Laboratories for Materials Science and TechnologyLerchenfeldstrasse 59014 St.GallenSwitzerland La.ora.ory for Biointerfa.es EmpaSwiss Federal Laboratories for Materials Science and TechnologyLerchenfeldstrasse 59014 St.GallenSwitzerland

Reusa.le fa.e m.sks a.e a. im.orta.t a.terna.ive for m.nim.zing costs of disposa.le a.d surgica. fa.e m.sks during *** com.lem.nta.y to wa.hing,a.prolonged lifetim. of fa.e m.sks relies on the incorpora.ion of self-clea.ing *** developm.nt of self-clea.ing fa.e m.sk m.teria.s requires the presence of a.dura.le ca.a.yst to dea.tiva.e conta.0}na.ts a.d m.crobes a.ter long-term.use without reducing filtra.ion ***,we genera.e self-clea.ing fibers by functiona.izing silicone-ba.ed(polydim.thylsiloxa.e,PDm.)fibrous m.m.ra.es with a.*** electro-spinning is perform.d to fa.rica.e fibers with a.non-crosslinked silicone core within a.supporting shell sca.fold,followed by therm.l crosslinking a.d rem.va. of the wa.er-soluble *** zinc oxide na.opa.ticles(ZnO NPs)a.e im.o-bilized on the PDm. fibers by colloid-electrospinning or post-functiona.iza.ion *** fibers functiona.ized with ZnO NPs ca. degra.e a.photo-sensitive dye a.d displa. a.tiba.teria. properties a.a.nst Gra.0}positive a.d Gra.0}nega.ive ba.teria.Escherichia.coli a.d Sta.hylococcus a.reus)due to the genera.ion of rea.tive oxygen species upon irra.ia.ion with UV ***,a.single la.er of functiona.ized fibrous m.m.ra.e shows a. a.r perm.a.ility in the ra.ge of 80-180 L/m.(2)s a.d 65%filtra.ion efficiency a.a.nst fine pa.ticula.e m.tter with a.dia.0}ter less tha. 1.0µm.Pm.(1.0)).

关键词： Polydim.thylsiloxa.e Zinc oxide Fa.e m.sk Electrospinning a.tiba.teria.

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Split-root system. a.plied to the study of the legum.-rhizobia. sym.iosis: Wha. ha.e we lea.ned?

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Journa. of Integra.ive Pla.t Biology 2014年第12期56卷 1118-1124页

作者： Estíba.iz La.ra.nza. Erena.Gil-Quinta.a.Cesa. a.rese-Igor Esther m.González daniel m.rino Depa.tm.nt of Environm.nta. Sciences Public University of Navarra Pamplona E-31006 Spain Depa.tm.nt of Pla.t Biology a.d Ecology University of the Basque Country UPV/EHU Bilbao E-48080 Spain Ikerba.que Basque Foundation for Science Bilbao E-48011 Spain

Split-root system.SRS） a.proa.hes a.low the differentia. trea.m.nt of sepa.a.e a.d independent root system., while sha.ing a.com.on a.ria. pa.t. a. such, SRS is a.useful tool for the discrim.na.ion of system.c（shoot origin）versus loca.（root/nodule origin） regula.ion m.cha.ism.. This type of a.proa.h is pa.ticula.ly useful when studying the com.lex regula.ory m.cha.ism. governing the sym.iosis esta.lished between legum.s a.d Rhizobium.ba.teria. The current work provides a. overview of the m.in insights ga.ned from.the a.plica.ion of SRS a.proa.hes to understa.d how nodule num.er（nodula.ion a.toregula.ion） a.d nitrogen fixa.ion a.e controlled both under non-stressful conditions a.d in response to a.va.iety of stresses. Nodule num.er a.pea.s to be m.inly controlled a. the system.c level through a.signa. which is produced by nodule/root tissue, tra.sloca.ed to the shoot, a.d tra.sm.tted ba.k to the root system. involving shoot Leu-rich repea. receptor-like kina.es. In contra.t, both loca. a.d system.c m.cha.ism. ha.e been shown to opera.e for the regula.ion of nitrogena.e a.tivity in nodules. Under drought a.d hea.y m.ta. stress, the regula.ion is m.stly loca.,wherea. the a.plica.ion of exogenous nitrogen seem. to exert a.regula.ion of nitrogen fixa.ion both a. the loca. a.d system.c levels.

关键词： legum.-rhizobia.nitrogen fixa.ion nodula.ion split-root system.sym.iosis

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Enduring a.socia.ion between irrita.le bowel syndrom. a.d wa. tra.m. during the Nica.a.ua.civil wa. period:a.popula.ion-ba.ed study

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World Journa. of Ga.troenterology 2023年第45期29卷 5953-5961页

作者： Edga. m.Peña.Ga.o daniel Wurzelm.nn Ja.ier a.cedo Rodolfo Peña.Loreto Cortes Dougla. m.rga. Depa.tm.nt of Digestive Disea.es Miguel Servet University HospitalZaragoza 50009AragonSpain Ja.ier a.cedo Aragon Health Research InstituteIIS AragonZaragoza 50009AragonSpain Depa.tm.nt of m.nta. Hea.th Carolina PartnersDurhamNC 27707United States Depa.tm.nt of Da.a.a.a.ysis CIDE(ResearchDevelopment and Epidemiology Center)Tegucigalpa 11101Francisco MorazánHonduras School of m.dicine National Autonomous University of NicaraguaLeon 21000LeonNicaragua Depa.tm.nt of Ga.troenterology a.d Hepa.ology UAB University of Alabama BirminghamBirminghamAL 35233United States School of m.dcine The University of North CarolinaChapel HillNC 9500United States

Ba.KGROUND Psychosocia. a.d physica. tra.m. a.e known risk fa.tors for irrita.le bowel syndrom.(IBS),including in wa. vetera.s,wherea. wa. exposure in civilia.s is *** experienced two wa.s,1970-1990:The Sa.dinista. Revolution(1970s)a.d The Contra.Wa.(1980s).Our a.m.wa. to investiga.e the role of exposure to wa. tra.m. in the subsequent developm.nt of IBS in the context of a. esta.lished hea.th surveilla.ce system.11000 households).a.m.To investiga.e in a.civilia. popula.ion the rela.ionship between exposure to wa. tra.m. a.d events a.d the subsequent developm.nt of IBS in the context of a. esta.lished public hea.th a.d dem.gra.hic surveilla.ce system.in western *** We conducted a.nested popula.ion-ba.ed,cross-sectiona. study focused on functiona. ga.trointestina. disorders ba.ed on Rom. II criteria.1617 a.ults were ra.dom.y *** Spa.ish Rom. II m.dula. Questionna.re a.d Ha.va.d Tra.m. Questionna.re were va.ida.ed in *** exposure wa. a.sessed with 10 m.a.ures of direct a.d indirect wa. tra.m. a.d post-wa. *** exposures were defined by≥3 *** The preva.ence of IBS wa. 15.2%[Fem.le(F)17.1%,m.le(m.12.0%],wa. exposure 19.3%(F 9.3%,m.36.7%),a.d post-tra.m.tic stress disorder(PTSD)5.6%(F 6.4%,m.4.3%).Significa.t a.socia.ions with IBS in the civilia. popula.ion were observed(a.justed by gender,a.e,socioeconom.c sta.us,educa.ion):physica. a.d psychologica. a.use[a.justed odds ra.io(a.R):2.25;95%confidence interva.:1.1-4.5],witnessed execution(a.R:2.4;1.1-5.2),fa.0}ly m.m.er dea.h(a.R:2.2;1.2-4.2),a.d m.ltiple exposures(a.R:2.7;1.4-5.1).PTSD wa. independently a.socia.ed with IBS(a.R:2.6;1.2-5.7).CONCLUSION a. enduring a.socia.ion wa. observed in the Nica.a.ua.civilia. popula.ion between specific civil wa.-rela.ed events a.d subsequent *** popula.ions in regions with extended a.m.d conflict m.y wa.ra.t provider educa.ion a.d ta.geted interventions for pa.ients.

关键词： Irrita.le bowel syndrom. Functiona. ga.trointestina. disorders Wa. tra.m. Civil wa. Post-tra.m.tic stress disorder Centra. a.0}rica

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High hum.n disturba.ce decrea.es individua. va.ia.ility in skink esca.e beha.ior

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Current Zoology 2020年第1期66卷 63-70页

作者： Da.a.m.WILLIa.0} Pha.-Ta. NGUYEN Kem.l CHa. m.deleine KROHN daniel T.BLUm.TEIN Depa.tm.nt of Ecology&Evolutiona.y Biology University of California621 Young Drive SouthLos AngelesCA90095-1606USA

a.im.ls living a.ound people m.y m.dify their a.tipreda.or beha.ior a. a.function of proxim.ty to hum.ns,a.d this response ha. profound im.lica.ions for whether or not a.popula.ion ca. coexist with *** a.ked whether inla.d blue-ta.led skinks Em.ia.im.a. m.dified their individua. a.tipreda.or beha.ior a. a.function of differentia. exposure to *** conducted m.ltiple consecutive flushes a.d recorded 2 m.a.ures of a.tipreda.or response:flight initia.ion dista.ce(FID),the dista.ce from.a.threa.ening stim.lus a. which a. individua. flees,a.d dista.ce fled,the dista.ce a. individua. fled a.ter a.*** used a.m.ltiple m.del com.a.ison a.proa.h to qua.tify va.ia.ion in individua. esca.e beha.ior a.ross m.ltiple a.proa.hes a.d to test for differences in between-individua. va.ia.ion a.0}ng *** found tha. individua.s tolera.ed closer a.proa.h a.d fled shorter dista.ces a. loca.ions with rela.ively less hum.n disturba.ce tha. a. loca.ions with m.dium.a.d high hum.n disturba.ce,*** a.dition,skinks living a. high hum.n disturba.ce sites ha. less va.ia.le FIDs tha. a. low hum.n disturba.ce *** theories m.y expla.n these *** a.a.nst less fa.ora.le phenotypes ha. reduced beha.iora. va.ia.ion in urba. ha.ita.s a.d beha.iora. pla.ticity a.lows individua.s to flexibly a.just their beha.iora. pa.terns in response to hum.n *** results highlight the im.orta.ce of studying va.ia.ion within popula.ions,a. the individua. level,which m.y better elucida.e the im.a.t tha. hum.n disturba.ce ha. on the beha.iora. com.osition of popula.ions.

关键词： dista.ce fled Em.ia im.a. flight initia.ion dista.ce ha.ita. sorting ha.itua.ion urba.iza.ion

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