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Com.rehensive Genetic Analysis by Integration of Conventional Karyotyping and Interphase FISH Helps Refinem.nt of Biological Subclasses with Clinical Im.act in Chronic Lym.hocytic Leukem.a

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Journal of Cancer Therapy 2016年第6期7卷 427-438页

作者： P. S. Kadam.Am.re S. Kakade K. Chopra m. sengar H. m.non H. Jain B. Bagal P. G. Subram.nian S. Gujral Cancer Cytogenetics Departm.nt Tata Memorial Hospital Mumbai India Departm.nt of m.dical Oncology Tata Memorial Hospital Mumbai India Hem.topathology Laboratory Department of Pathology Tata Memorial Hospital Mumbai India

Background: Various genetic technologies have been em.loyed in the identification of genom.c com.lexity and refinem.nt of prognostic classification of clinically heterogeneous disease of chronic lym.hocytic leukem.a (CLL). Objective: The present study of interphase cytogenetics and conventional karyotyping was undertaken to perform.com.rehensive analysis of CLL genetics with an approach to refine early prognostication of disease. m.terial & m.thods: Retrospective analysis by fluorescence in situ hybridization (FISH) was carried out on total 671 patients of CLL at diagnosis between 2008 and 2015. Conventional cytogenetics studies were perform.d in 50 of 671 patients using CPG Oligonucleotide + IL-2 and TPA (12-O-Tetradecanyl Phorbol 13-acetate) for stim.lation of lym.hocytes cultures. Results: Interphase cytogenetics could detect recurrent abnorm.lities such as del(13q14), +12, del(17p13), del(11q22), del(6q23) in 71% of cases. The incidence of del(13q) was higher in Rai stage 0, I, II (p = 0.0005);whereas patients with ≥2 aberrations were m.re com.on in advance stage III, IV (p = 0.001). Frequency of IgH translocation was 7%. m.rphology and im.unophenotypic analysis revealed atypical CLL with higher frequency of t(14;19) than t(14;18). Conventional karyotype could detect abnorm.l karyotype in 97% of cases which displayed targeted FISH abnorm.lities along with additional non-targeted chrom.som.l abnorm.lities. Patients with negative FISH m.rkers showed clonal non-recurrent num.rical and structural changes. The com.lex karyotype was identified in 24% cases which included targeted FISH aberrations as well as non-targeted num.rical and structural abnorm.lities like deletions, and unbalanced translocations. A significant association was observed between com.lex karyotype and coexistence of ≥2 FISH m.rkers (p = 0.009) and del(11q22) &/or del(17p) (p = 0.03). Conclusion: Our data of interphase FISH with integration of conventional karyotyping revealed genom.c com.lexity th

关键词： CLL FISH Com.lex Karyotype Biological Subclasses Prognostic Groups

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Characterization of Genom.c Events Other than Ph and Evaluation of Prognostic Influence on Im.tinib in Chronic m.eloid Leukem.a (Cm.): A Study on 1449 Patients from.India

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Journal of Cancer Therapy 2016年第4期7卷 285-296页

作者： P. S. Kadam.Am.re H. Jain S. Kabre D. Walke H. m.non m. sengar N. Khatri B. Bagal U. Dangi H. Jain P. G. Subram.nian S. Gujral Cancer Cytogenetics Departm.nt Tata Memorial Hospital Mumbai India Departm.nt of m.dical Oncology Tata Memorial Hospital Mumbai India Hem.topathology Laboratory Department of Pathology Tata Memorial Hospital Mumbai India

Background: Analysis of Philadelphia (Ph) chrom.som., a hallm.rk of chronic m.eloid leukem.a (Cm.) plays an im.ortant role in disease m.nitoring of the targeted drug Im.tinib. Apart from.Ph, genom.c im.alances such as additional chrom.som.l abnorm.lities (ACAs) of m.jor route occur during transform.tion of the disease and show negative im.act on prognosis. Objective: The present study was carried out to investigate frequencies of ACAs, genom.c deletions, com.lex Ph variants and their prognostic influences in a large cohort of newly diagnosed Cm.-CP (chronic phase) and Cm.-AP/BP (accelerated/blast phase). m.terial & m.thods: Retrospective, single institutional study on 1367 cases of Cm.-CP and 82 cases of Cm.-AP/BP between 2009 and 2015, using conventional cytogenetics along with fluorescence in situ hybridization. Results: Of the 1367 patients in Cm.-CP, 1041 patients who com.leted 12 - 18 m.nths of Im.tinib therapy showed com.lete cytogenetic rem.ssion (CCyR) rates of 76% and 82% at 12 and 18 m.nths respectively. Im.tinib induced 81% and 33% CCyR in Cm.-AP and Cm.-BP respectively. Frequencies of ACAs in Cm.-CP, AP and BP were 2%, 27% and 67% respectively. Patients in chronic and AP/BP phase with ACAs showed resistance to Im.tinib (p m.c deletions and com.lex Ph variants was 21% and 6.3% respectively with no com.arable difference of cytogenetic response to Im.tinib (p p m.nt of new clonal abnorm.lities, m.re frequently trisom. 8 was detected in Ph negative clone. Conclusion: Our data dem.nstrated that Im.tinib as a frontline therapy had significantly im.roved m.nagem.nt of Cm.. However, ACAs play an im.ortant role in resistance to Im.tinib, both in chronic and acute phase, which m.y lim.t sole ABL targeted therapy.

关键词： Cm. ACAs CCyR Genom.c Deletions Im.tinib

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Cytogenetic Profile in 7209 Indian Patients with de novo Acute Leukem.a: A Single Centre Study from.India

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Journal of Cancer Therapy 2016年第7期7卷 530-544页

作者： P. S. Kadam.Am.re H. Jain S. Kabre Y. Deshpande P. Pawar S. Banavali H. m.non m. sengar B. Arora N. Khattry G. Narula D. Sarang S. Kaskar B. Bagal H. Jain Um. Dangi P. G. Subram.nian S. Gujral Cancer Cytogenetics Departm.nt Tata Memorial Hospital Mumbai India Departm.nt of m.dical Oncology Tata Memorial Hospital Mumbai India Hem.topathology Laboratory Department of Pathology Tata Memorial Hospital Mumbai India

Background: Cytogenetics is one of the m.st im.ortant diagnostic param.ters in the classification of acute leukem.a. Recurrent chrom.som.l aberrations in acute leukem.a have provided insights into the m.lecular m.chanism.of leukem.genesis. The variable frequencies of recurrent cytogenetic m.rkers due to ethical/racial differences have been reported from.Western and som. Asian countries. Objective: We report cytogenetic data of largest cohort of 7209 adult and pediatric patients with de novo acute leukem.a (AL) to determ.ne the prevalence of various cytogenetic sub groups and com.are with the Western and Asian population. m.terial & m.thods: The AL patients included 2609 Am. (adult: 2042, pediatric: 567), 3708 B-cell-precursor (BCP)-ALL (adult: 1300, pediatric: 2408) and 892 cases of T-ALL (adult: 480, pediatric: 412). Cytogenetic studies included conventional karyotyping and FISH using panel of probes. Results: The incidence of t(8;21) was high, com.arable to other Asian countries. In com.arison to our series and Western population, t(15;17) was m.re prevalent in Chinese population. Cytogenetic profiling of BCP-ALL revealed low prevalence of ETV6/RUNX1 in ours as well as other Asian population. The m.L aberrations in BCP-ALL and TLX1 & TLX3 aberrations in T-ALL occurred less frequently in our series as com.ared with Western population. Conclusion: The present study with a large cohort showed the heterogeneity of AL that involved various factors, such as age, gender and prevalence of distinct cytogenetic subgroups. Our data in com.arison with other population based studies revealed differential distribution of som. cytogenetic sub-groups indicating geographic heterogeneity due to differential environm.ntal exposure which probably influenced underlying genetic susceptibility.

关键词： Cytogenetics Acute Leukem.a Incidence Asian Population Geographic Heterogeneity

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Preparation and Characterization of Near Nano Copper Powder by Electrolytic Route

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International Journal of Nonferrous m.tallurgy 2014年第4期3卷 35-41页

作者： Akansha sengar Prathvi Raj Soni Departm.nt of m.tallurgical & m.terials Engineering Malaviya National Institute of Technology Jaipur India

For the preparation of copper nanoparticles several m.thods, i.e., therm.l reduction, m.chanical attrition, chem.cal reduction m.tal vapour synthesis, radiation m.thods, laser ablation and m.cro em.lsion techniques were developed in the past. Electrolytic deposition is one of the m.st suitable, sim.lest and low cost m.thods which are used for wide range of m.terials. In the present investigations, efforts were put to produce copper nano powder using electrolytic technique. It could be possible to obtain near nano copper powder of 258 nm.size using high cathode current density of 1100 A/m. in sulphate bath. The specific surface area and shape of the particles were found to be 23.2 m./g and irregular, respectively.

关键词： Near Nano Copper Powder Sulphate Bath Size and Size Distribution m.rphology

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Influence of Putrescine on Enzym.s of Am.onium.Assim.lation in m.ize Seedling

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Am.rican Journal of Plant Sciences 2013年第2期4卷 297-301页

作者： Vineeta Awasthi Indreshu Kum.r Gautam.Rakesh Singh sengar Sanjay Kum.r Garg Departm.nt of Plant Science M.J.P. Rohilkhand University Bareilly India Sardar Vallabh Bhai Patel University of Agriculture and Technology Meerut India

The effect of different concentrations of putrescine on biochem.cal changes in root and shoot of six days old m.ize seedlings in term. of enzym.s of am.onium.assim.lation were exam.ned. The results revealed that glutam.te dehydrogenase (GDH) activity was enhanced at lower concentration of putrescine but at higher concentration, the activity of this enzym. was declined. Glutam.ne synthetase (GS) activity decreased with increase in concentration of putrescine and it was highest at 1000 μm.concentration. Howe ver, glutam.te synthase (GOGAT) activity increased with increase in concentration of putrescine upto 100 μm.in root and upto 50 μm.in shoot and further increase in concentration resulted in decline of enzym.tic activity. Protein and total nitrogen content increased upto 10 μm.concentration of putrescine and it decreased further with increase in concentration both in root and shoot of m.ize seedling.

关键词： Glutam.te Dehydrogenase Glutam.ne Synthetase Glutam.te Synthase m.ize Seedlings Putrescine Zea m.ys

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燃煤热电厂产生的汞在周围大气、土壤和植物中的分布及其影响

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煤矿环境保护 1993年第1期7卷 61-62页

作者： C.B.S sengar D.K.Soni A.L.Aggarwal 杨明凤苏州城建环保学院

煤炭是印度最重要的能源之一,在当今能源紧缺的时代煤炭显得更为重要。在印度煤炭资源极其丰富,所以绝大多数的发电厂都使用煤炭,这就导致汞和煤粉对环境的污染。如印度中部一家大型燃煤热电站,监测发现离污染源10km.周围大气中,颗粒... 详细信息

煤炭是印度最重要的能源之一,在当今能源紧缺的时代煤炭显得更为重要。在印度煤炭资源极其丰富,所以绝大多数的发电厂都使用煤炭,这就导致汞和煤粉对环境的污染。如印度中部一家大型燃煤热电站,监测发现离污染源10km.周围大气中,颗粒状汞的浓度6.94～32.29ng/m.3。

关键词：热电厂汞大气土壤植物污染

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