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RPLP0/TBP are the most stable reference genes for human dental pulp stem cells under osteogenic differentiation

World Journal of stem Cells 2024年第6期16卷 656-669页

作者： Daniel B Ferreira Leticia m Gasparoni Cristiane F Bronzeri Katiucia B s Paiva Laboratory of Extracellular matrix Biology and Cellular Interaction Department of AnatomyInstitute of Biomedical SciencesUniversity of São PauloSão Paulo 05508-000São PauloBrazil

BACKGROUND Validation of the reference gene(RG)stability during experimental analyses is essential for correct quantitative real-time polymerase chain reaction(RT-qPCR)data ***,in an unreliable way,several studies use genes involved in essential cellular functions[glyceraldehyde-3-phosphate dehydro-genase(GAPDH),18s rRNA,andβ-actin]without paying attention to whether they are suitable for such experimental conditions or the reason for choosing such ***,such studies use only one gene when minimum Information for Publication of Quantitative Real-Time PCR Experiments guidelines recom-mend two or more *** impacts the credibility of these studies and causes dis-tortions in the gene expression *** tissue engineering,the accuracy of gene expression drives the best experimental or therapeutical *** cultivated DPsCs under two conditions:Undifferentiated and osteogenic dif-ferentiation,both for 35 *** evaluated the gene expression of 10 candidates for RGs[ribosomal protein,large,P0(RPLP0),TATA-binding protein(TBP),GAPDH,actin beta(ACTB),tubulin(TUB),aminolevulinic acid synthase 1(ALAs1),tyro-sine 3-monooxygenase/tryptophan 5-monooxygenase activation protein,zeta(YWHAZ),eukaryotic translational elongation factor 1 alpha(EF1a),succinate dehydrogenase complex,subunit A,flavoprotein(sDHA),and beta-2-micro-globulin(B2m)]every 7 d(1,7,14,21,28,and 35 d)by *** data were analysed by the four main algorithms,ΔCt method,geNorm,NormFinder,and BestKeeper and ranked by the RefFinder *** subdivided the samples into eight *** All of the data sets from clonogenic and osteogenic samples were analysed using the RefFinder *** final ranking showed RPLP0/TBP as the two most stable RGs and TUB/B2m as the two least stable *** theΔCt method or NormFinder analysis showed TBP/RPLP0 as the two most stable ***,geNorm analysis showed RPLP0/EF1αin the first *** algorithms’two least stab

关键词： Dental pulp stem cells Reference gene Housekeeping gene Endogenous gene Osteogenic differentiation RefFinder

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Artificial Intelligence Prediction of One-Part Geopolymer Compressive strength for sustainable Concrete

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Computer modeling in Engineering & sciences 2024年第10期141卷 525-543页

作者： mohamed Abdel-mongy mudassir Iqbal m.Farag Ahmed.m.Yosri Fahad Alsharari saif Eldeen A.s.Yousef Department of Civil Engineering College of EngineeringJouf UniversitySakaka72388Saudi Arabia Department of Civil Engineering University of Engineering and TechnologyPeshawar54890Pakistan Civil Engineering Department Faculty of EngineeringAl-Azhar UniversityCairo11884Egypt Civil Engineering Department Faculty of EngineeringAswan UniversityAswan81528Egypt

Alkali-activated materials/geopolymer(AAms),due to their low carbon emission content,have been the focus of recent studies on ecological *** terms of performance,fly ash and slag are preferredmaterials for precursors for developing a one-part ***,determining the optimum content of the input parameters to obtain adequate performance is quite challenging and scarcely ***,in this study,machine learning methods such as artificial neural networks(ANN)and gene expression programming(GEP)models were developed usingmATLAB and GeneXprotools,respectively,for the prediction of compressive strength under variable input materials and content for fly ash and slag-based one-part *** database for this study contains 171 points extracted from literature with input parameters:fly ash concentration,slag content,calcium hydroxide content,sodium oxide dose,water binder ratio,and curing *** performance of the two models was evaluated under various statistical indices,namely correlation coefficient(R),mean absolute error(mAE),and rootmean square error(RmsE).In terms of the strength prediction efficacy of a one-part geopolymer,ANN outperformed *** and parametric analysis were also performed to identify the significant contributor to *** to a sensitivity analysis,the activator and slag contents had the most effects on the compressive strength at 28 *** water binder ratio was shown to be directly connected to activator percentage,slag percentage,and calcium hydroxide percentage and inversely related to compressive strength at 28 days and curing temperature.

关键词： Artificial intelligence techniques one-part geopolymer artificial neural network gene expression modelling sustainable construction polymers

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metabolomics for quality assessment of poultry meat and eggs

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Food Quality and safety 2024年第1期8卷 27-40页

作者： m.s.Yogeswari Jinap selamat Nuzul Noorahya Jambari Alf Khatib mohd Hishammfariz mohd Amin suganya murugesu Laboratory of Food safety and Food Integrity Institute of Tropical Agriculture and Food SecurityUniversiti Putra MalaysiaSerdangSelangor Darul EhsanMalaysia Department of Food science Faculty of Food Science and TechnologyUniversiti Putra MalaysiaSerdangSelangor Darul EhsanMalaysia Department of Pharmaceutical Chemistry Kulliyyah of PharmacyInternational Islamic University MalaysiaKuantanPahang Darul MakmurMalaysia Faculty of Pharmacy Airlangga UniversitySurabayaIndonesia Department of Veterinary service malaysia Federal Government Administration CentrePutrajayaMalaysia

The poultry industry is experiencing rapid growth *** accelerated growth has led to multiple food fraud incidents across the food supply chain,which consequently created a demand for precise determination of quality poultry *** increase in demand for precise poultry production quality has necessitated advanced *** has emerged as a viable solution by offering detailed differentiation of biochemical indicators throughout the poultry supply ***,this study provides a means to address risk factors affecting the poultry industry without compromising animal welfare,which is a critical *** review focuses on important issues related to poultry product quality *** adulteration has escalated in recent years as it is driven by the increasing focus on consuming high-quality and nutritious ***,there is no specifc guideline for such determinations,especially when appearance,texture,and taste can be manipulated by substituting for food *** can pave the way for a deeper understanding of existing and novel biochemical indicators responsible for determining the quality of poultry meat and *** approach holds the potential to enhance the overall quality of poultry meat and egg products while also preventing food fraud.

关键词： Poultry metabolomics welfare application authentication.

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Effects of low molecular weight polysaccharide from sargassum thunbergii against palmitic acid-induced intracellular lipid accumulation in 3T3-L1 adipocyte and HepG2 cells

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Food science and Human Wellness 2024年第4期13卷 2244-2252页

作者： Hyo-Geun Lee D.P.Nagahawatta m.J.m.s.Kurera Kyung-mo song Yun-sang Choi You-Jin Jeon min-Cheol Kang Department of marine Life sciences Jeju National UniversityJeju 63243Republic of Korea Department of Biotechnology Faculty of Agriculture and Plantation ManagementWayamba University of Sri LankaMakanduraGonawila(NWP)60170Sri Lanka Research Group of Food Processing Korea Food Research Institute245Wanju 55365Republic of Korea

Low molecular weight polysaccharides can be isolated from sargassum thunbergii(LmPsT)and in vitro experiments were conducted to evaluate the inhibitory effects on *** natures of LmPsT were attained from *** and appraised their LmPsT on palmitic acid(PA)induced lipid accretion in Hep G2,and 3T3-L1 *** treatment lessened lipid deposition and intracellular free fatty acid and triglyceride intensities in PA-treated above mentioned *** mechanistic study publicized that LmPsT2 significantly suppressed adipogenesis and stimulated the PA-treated 3T3-L1 cells occupied in the lipolysis ***,in PA-treated Hep G2 cells,the free fatty acid oxidation was significantly increased by *** these constructive properties of LmPsT2 from ***,is a potential candidate for diminishing the intracellular lipids,and for a therapeutic agent in those conditions.

关键词： sargassum thunbergii Low molecular weight polysaccharide Lipid accumulation Obesity Liver steatosis

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Natural Convection and Irreversibility of Nanofluid Due to Inclined magnetohydrodynamics(mHD)Filled in a Cavity with Y-shape Heated Fin:FEm Computational

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Computer modeling in Engineering & sciences 2024年第5期139卷 1505-1519页

作者： Afraz Hussain majeed Rashid mahmood sayed m.Eldin Imran saddique s.saleem muhammad Jawad school of Energy and Power Engineering Jiangsu UniversityZhenjiang212013China Department of mathematics Air UniversityIslamabad44000Pakistan Center of Research Faculty of EngineeringFuture University in EgyptNew Cairo11835Egypt Department of mathematics University of Management and TechnologyLahore54770Pakistan Department of mathematics University of SargodhaSargodha40100Pakistan Department of mathematics College of ScienceKing Khalid UniversityAbha61413Saudi Arabia Faculty of sciences The Superior UniversityLahore54000Pakistan

This study explains the entropy process of natural convective heating in the nanofluid-saturated cavity in a heated fin andmagnetic *** temperature is constant on the Y-shaped fin,insulating the topwall while the remaining walls remain *** walls are subject to impermeability and non-slip *** mathematical modeling of the problem is demonstrated by the continuity,momentum,and energy equations incorporating the inclined magnetic *** elucidating the flow characteristics Finite Elementmethod(FEm)is implemented using stable FE pair.A hybrid fine mesh is used for discretizing the *** and thermal plots concerning parameters are *** addition,a detailed discussion regarding generation energy by monitoring changes in magnetic,viscous,total,and thermal irreversibility is *** addition,line graphs are created for the u and v components of the velocity profile to predict the flow *** simulations assume the dimensionless representative of magnetic field Hartmann number Ha between 0 and 100 and a magnetic field inclination between 0 and 90 degrees.A constant 4% volume proportion of nanoparticles is employed throughout all scenarios.

关键词： Finite element method nanomaterials entropy mHD square cavity Y-fin

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Nanomotion of bacteria to determine metabolic profile

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Nanotechnology and Precision Engineering 2024年第1期7卷 1-9页

作者： s.N.Pleskova E.V.Lazarenko N.A.Bezrukov R.N.Kriukov A.V.Boryakov m.E.Dokukin s.I.surodin Research Laboratory of scanning Probe microscopy Lobachevsky State University of Nizhny NovgorodNizhny Novgorod 603950Russia Department of Nanotechnology and Biotechnology Nizhny Novgorod State Technical University n.a.R.E.AlekseevNizhny Novgorod 603950Russia sarov Physics and Technology Institute National Research Nuclear University MEPhISarov 607186Russia

In addition to their visible motion such as swimming(e.g.,with the help ofﬂagella),bacteria can also exhibit nanomotion that is detectable only with highly sensitive instruments,and this study shows that it is possible to detect bacterial nanomotion using an AFm detection *** results show that the nanomotion characteristics depend on the bacterial strain,and that nanomotion can be used to sense the metabolic activity of bacteria because the oscillations are sensitive to the food preferences of the bacteria and the type of surrounding medium.

关键词： Nanomotion Bacteria Cantilever Oscillation Atomic force microscopy metabolism

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Planetary Homeostasis of Reactive Nitrogen Through Anaerobic Ammonium Oxidation

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Engineering 2024年第7期38卷 175-183页

作者： Guibing Zhu Bangrui Lan shuci Liu Cameron m.Callbeck shanyun Wang Liping Jiang Asheesh Kumar Yadav Jan Vymazal mike s.m.Jetten Ganlin Zhang Yongguan Zhu Research Center for Eco-Environmental sciences Chinese Academy of SciencesBeijing 100085China Department of Environment and science Queensland GovernmentBrisbaneQLD 4102Australia Department of Environmental sciences University of BaselBasel 4056Switzerland CsIR-Institute of minerals and materials Technology Bhubaneswar 751013India Faculty of Environmental sciences Czech University of Life Sciences PraguePraha 16521Czech Republic Department of microbiology Radboud UniversityNijmegen 6525 XZThe Netherlands Key Laboratory of Watershed Geographic sciences Nanjing Institute of Geography and LimnologyChinese Academy of SciencesNanjing 210008China state Key Laboratory of soil and sustainable Agriculture Institute of Soil ScienceChinese Academy of SciencesNanjing 210008China University of Chinese Academy of sciences Beijing 100049China

The availability of nitrogen(N)is crucial for both the productivity of terrestrial and aquatic ecosystems ***,the overuse of artificial fertilizers and the energy required to fix nitrogen have pushed the global nitrogen cycle(N-cycle)past its safe operating limits,leading to severe nitrogen pollution and the production of significant amounts of greenhouse gas nitrous oxide(N2O).The anaerobic ammonium oxidation(anammox)mechanism can counteract the release of ammonium and N2O in many oxygenlimited situations,assisting in the restoration of the homeostasis of the Earth’s N *** this work,we looked into the characteristics of the anammox hotspots’distribution across various types of ecosystems *** hotspots are present at diverse oxic-anoxic interfaces in terrestrial systems,and they are most prevalent at the oxic-anoxic transition zone in aquatic *** on the discovery of an anammox hotspot capable of oxidizing ammonium anoxically into N2 without N2O by-product,we then designed an innovative concept and technical routes of nature-based anammox hotspot geoengineering for climate change,biodiversity loss,and efficient utilization of water *** 15 years of actual use,anammox hotspot geoengineering has proven to be effective in ensuring clean drinking water,regulating the climate,fostering plant and animal diversity,and enhancing longterm environmental *** sustainable biogeoengineering of anammox could be a workable natural remedy to resolve the conflicts between environmental pollution and food security connected to N management.

关键词： Biogeochemical N-cycle Oxic-anoxic interface Nature-based solution Biogeoengineering Nitrogen sustainable development

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Rare earth based mgPm_(2)X_(4)(X=s,se) spinel chalcogenides for spintronic and thermoelectric applications

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Journal of Rare Earths 2024年第8期42卷 1577-1585,I0006页

作者： Tariq m.Al-Daraghmeh Omar Zayed Ghulam m.mustafa Taharh Zelai Bisma Younas Hind Albalawi s.Bouzgarrou Othman Hakami Q.mahmood Khaild I.Hussein Basic sciences Department King Abdullah Ⅱ School of EngineeringPrincess Sumaya University For TechnologyP.O.Box 1438Al-jubaiha11941AmmanJordan Department of Physics Division of Science&TechnologyUniversity of EducationLahorePakistan Department of Physics Faculty of ScienceJazan University JazanSaudi Arabia Department of Physics The University of LahoreLahorePakistan Department of Physics College of SciencesPrincess Nourah bint Abdulrahman University(PNU)P.O.Box 84428Riyadh11671Saudi Arabia Department of Physics College of ScienceQassim UniversityPo.64BuraidahSaudi Arabia Laboratoire de microelectronique et Instrumentation(UR 03/13-04) Faculte des Sciences de MonastirAvenue de I'Environnement 5000MonastirTunisia Department of Chemistry Faculty of ScienceJazan University JazanSaudi Arabia Basic&Applied scientific Research Center Department of PhysicsCollege of ScienceImam Abdulrahman Bin Faisal UniversityP.O.Box 1982Dammam 31441Saudi Arabia Department of Radiological sciences College of Applied Medical SciencesKing Khalid UniversityAbha61421Saudi Arabia

In current report,the structural,magnetic,and thermoelectric properties of RE doped mgPm_(2)X_(4)(X=s,se) spinels were *** energy difference in ferromagnetic and antiferromagnetic states reveals the stability of mgPm_(2)(s/se)_(4) in the ferromagnetic *** co mputation of enthalpy of formation also ascertains thermodynamic stability of crystal ***-dependent band structure and density of states analysis reveal ferromagnetic semiconducting character showing different electronic behavior in both spin *** room temperature ferromagnetism,spin polarization and Curie temperature are estimated from exchange energies *** addition,exchange constants(N_(0)α and N_(0)β),exchange energy Δ_(x)(pd),crystal ifeld energy,and double exchange mechanism were studied to explore the magnetic ***,the electrical conductivity,thermal conductivity,seebeck co-efficient,and power factor show effect on electrons spin and their potential for thermoelectric devices.

关键词： Rare earth elements spintronic applications Thermoelectric effects Exchange energies

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Laser processing effects on Ti−45Nb alloy surface,corrosive and biocompatible properties

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Transactions of Nonferrous metals society of China 2024年第8期34卷 2533-2551页

作者： I.CVIJOVIĆ-ALAGIĆ s.LAKETIĆ m.mOmČILOVIĆ J.CIGANOVIĆ Đ.VELJOVIĆ J.BAJAT V.KOJIĆ m.RAKIN Vinča Institute of Nuclear sciences-National Institute of thеRepublic of serbia University of BelgradeP.O.Box 52211001 BelgradeSerbia Faculty of Technology and metallurgy University of BelgradeKarnegijeva 411120 BelgradeSerbia Oncology Institute of Vojvodina Faculty of MedicineUniversity of Novi SadPut Dr Goldmana 421204 Sremska KamenicaSerbia

The Ti−45Nb(wt.%)alloy properties were investigated in relation to its potential biomedical *** surface modification was utilized to improve its performance in biological *** a result of the laser treatment,(Ti,Nb)O scale was formed and various morphological features appeared on the alloy *** electrochemical behavior of Ti−45Nb alloy in simulated body conditions was evaluated and showed that the alloy was highly resistant to corrosion deterioration regardless of additional laser surface modification ***,the improved corrosion resistance after laser treatment was evident(the corrosion current density of the alloy before laser irradiation was 2.84×10^(−8)A/cm2,while that after laser treatment with 5 mJ was 0.65×10^(−8)A/cm2)and ascribed to the rapid formation of a complex and passivating bi-modal surface oxide *** cytotoxicity and effects of the Ti−45Nb alloy laser surface modification on the mRC-5 cell viability,morphology,and proliferation were also *** Ti−45Nb alloy showed no cytotoxic ***,cells showed improved viability and adherence to the alloy surface after the laser irradiation *** highest average cell viability of 115.37%was attained for the alloy laser-irradiated with 15 *** showed that the laser surface modification can be successfully utilized to significantly improve alloy performance in a biological environment.

关键词： Ti−45Nb alloy laser surface scanning electrochemical properties biocompatibility cell morphology

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A review on transition metal oxides based photocatalysts for degradation of synthetic organic pollutants

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Journal of Environmental sciences 2024年第5期139卷 389-417页

作者： Athira Krishnan Anna swarnalal Divine Das midhina Krishnan Viswanathan s.saji smAshibli Department of Chemistry Amrita Vishwa VidyapeethamAmritapuriKerala690525India Interdisciplinary Research Center for Advanced materials King Fahd University of Petroleum&MineralsDhahran31261Saudi Arabia Department of Chemistry University of KeralaKariavattom CampusThiruvananthapuramKerala695581India

This review provides insight into the current research trend in transition metal oxides(TmOs)-based photocatalysis in removing the organic colouring matters from *** easy understanding,the research progress has been presented in four generations according to the catalyst composition and mode of application,viz:single component TmOs(the firstgeneration),doped TmOs/binary TmOs/doped binary TmOs(the second-generation),inactive/active support-immobilized TmOs(the third-generation),and ternary/quaternary compositions(the fourth-generation).The first two generations represent suspended catalysts,the third generation is supported catalysts,and the fourth generation can be suspended or *** review provides an elaborated comparison between suspended and supported catalysts,their general/specific requirements,key factors controlling degradation,and the methodologies for performance *** the plausible fundamental and advanced dye degradation mechanisms involved in each generation of catalysts were *** existing challenges in TmOs-based photocatalysis and how the researchers approach the hitch to resolve it effectively are *** research trends are also presented.

关键词： Photocatalysis Dye degradation Transition metal oxides and composites supported catalysts suspended catalysts

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