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甜菜种子中线粒体的纯化研究

中山大学学报（自然科学版） 2000年第3期39卷 78-81页

作者：宋松泉 Kenneth m.FREDLUND Ian m.mφller 中山大学生命科学学院广东广州510275 Department of Seed Physiology Novartis Seeds AB Department of Plant Physiology Lund University

吸胀 2 4h的甜菜 (BetavulgarisL cv Loke)种子线粒体通过差速离心和修改的Percoll梯度 (上部为 0 3mol/L甘露醇 ,下部为 0 3mol/L蔗糖 )被纯化 .线粒体位于Percoll梯度的上部 ,大约 30～ 35mL处 .细胞色素C氧化酶 (线粒体的标记酶 )... 详细信息

吸胀 2 4h的甜菜 (BetavulgarisL cv Loke)种子线粒体通过差速离心和修改的Percoll梯度 (上部为 0 3mol/L甘露醇 ,下部为 0 3mol/L蔗糖 )被纯化 .线粒体位于Percoll梯度的上部 ,大约 30～ 35mL处 .细胞色素C氧化酶 (线粒体的标记酶 )活性、苹果酸脱氢酶活性和蛋白质含量主要分布在此部位 .

关键词：线粒体纯化 Percoll梯度甜菜种子

A patchy-saturated rock physics model for tight sandstone based on microscopic pore structures

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Applied Geophysics 2022年第2期19卷 147-160,306页

作者： Wu Chun-Fang Ba Jing Carcione José m. müller Tobias m. Zhang Lin School of Earth Sciences and Engineering Hohai UniversityNanjing 211100China National Institute of Oceanography and Applied Geophysics(OGS) Trieste 34010Italy

The wave-induced local fluid flow mechanism is relevant to the complex heterogeneity of pore structures in *** analysis of the local fluid flow mechanism is useful for accurately describing the wave propagation characteristics in reservoir *** the exploration and production of hydrocarbon reservoirs,the real stratum may be partially saturated with a multi-phase fluid mixture in ***,it is of great significance to investigate the wave velocity dispersion and attenuation features in relation to pore structures and *** this work,the characteristics of fabric microstructures are obtained on the basis of pressure dependency of dry rock moduli using the effective medium theory.A novel anelasticity theoretical model for the wave propagation in a partially-saturated medium is presented by combining the extended Gurevich squirt-flow model and White patchysaturation *** simulations are used to analyze wave propagation characteristics that depend on water saturation,external patchy diameter,and *** consider a tight sandstone from the Qingyang area of the Ordos Basin in west China and perform ultrasonic measurements under partial saturation states and different confining pressures,where the basic properties of the rock are obtained at the full gas *** comparison of experimental data and theoretical modeling results shows a fairly good agreement,indicating that the new theory is effective.

关键词： Pore structure patchy saturation state squirt-flow effect wave velocity dispersion and attenuation

Acoustic-electrical properties and rock physics models for shale-oil formations:prediction of reservoir properties of interbedded sandstone and shale layers

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Applied Geophysics 2022年第4期19卷 485-502,603页

作者： Pang meng-Qiang Ba Jing Wu Chun-Fang Carcione José maria müller Tobias School of Earth Science and Engineering Hohai UniversityNanjingu211000China Bochum University of Applied Sciences D-44801 BochumGermany Shanghai Technical Institute of Electronics and Information Shanghai201411China National Institute of Oceanography and Applied Geophysics(OGS) SgonicoTrieste 34010

In recent years,the Yanchang shale-oil formations of the Ordos Basin are rich in reserves with complex lithology and structure characteristics,low porosity and low permeability,and weak anomalies for oil and water discriminations,have been the key targets of unconventional oil/gas resource exploration and development in the relevant *** joint acoustic-electrical(AE)properties can be used to interpret reservoir lithology,mineralogy,pore structure,and fluid *** conduct tests of thin section analysis,X-ray diff raction,and ultrasonic and electrical experiments at diff erent pressures and saturation degrees,cores from the shale-oil formations in the Q area of the basin are *** variations in AE properties with respect to clay content,porosity,pressure(microfracture),and saturation are *** experimental results indicate that the rock physics behaviors of sandstones with diff erent clay contents vary *** AE properties of clean sandstones are basically dependent on the microfractures(pressure),while for muddy sandstones,the clay content is an important factor affecting the *** target reservoir consists of interbedded sandstone and shale *** AE equivalent medium equations and the Gurevich theory are applied to establish the joint models for the diff erent lithologies and simulate the variations in AE properties with respect to fluid type,pore structure,and mineral *** three-dimensional joint templates of clean and muddy sandstones,as well as shale,are developed based on the elastic and electrical attributes and then calibrated using the experimental and well-log *** reservoir properties are estimated with the templates and validated by the log *** results indicate that the joint templates based on lithology characteristics can eff ectively characterize the properties of interbedded sandstone and shale ***,the combined application of AE data provides more beneficial informatio

关键词： shale-oil formations acoustic-electrical(AE)properties interbedded layers clay content pore structure rock physics model

温度对甜菜种子萌发速率和线粒体活性的影响(英文)

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中山大学学报（自然科学版） 2002年第1期41卷 59-61页

作者：宋松泉 FREDLUND K m mΦller I m 中山大学生命科学学院广东广州510275 Department of Plant Physiology Lund University Department of Seed Physiology Novartis Seeds AB

研究了不同温度对甜菜种子萌发速率和线粒体活性的影响。在 7,14和 2 0℃中甜菜种子萌发 5 0 %需要的时间分别是 7,3和 2 3d ;萌发 5 0 %的热时间大约是 46℃·d。在 7,14和 2 0℃萌发的种子和幼苗的线粒体中 ,总蛋白含量以及细胞色素... 详细信息

研究了不同温度对甜菜种子萌发速率和线粒体活性的影响。在 7,14和 2 0℃中甜菜种子萌发 5 0 %需要的时间分别是 7,3和 2 3d ;萌发 5 0 %的热时间大约是 46℃·d。在 7,14和 2 0℃萌发的种子和幼苗的线粒体中 ,总蛋白含量以及细胞色素氧化酶 (CCO)和苹果酸脱氢酶 (mDH)的活性随着萌发进程增加 ;相对分子质量大约为 1880 0 ,35 0 0 0和 36 0 0 0的蛋白质随着萌发进程降低 ;相对分子质量大约为 2 2 0 0 0的蛋白质在 7℃中逐渐增加 ,在 14和 2 0℃中则先少量增加然后下降。 2 0℃中萌发的种子和幼苗的线粒体的CCO和mDH的比活性和总活性比在 7和 14℃中高。这些结果表明 ,萌发温度显著地影响种子的萌发速率、线粒体的CCO和mDH活性以及较小相对分子质量热休克蛋白 2

关键词： mDH活性甜菜种子萌发速率温度线粒体 CCO活性萌发时间

Key Intermediates: A Simple and Highly Selective Synthesis of 5-Amino-1-aryl-1H-pyrazole-4-carbonitrilesfor Applications in the Crop Protection

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Advances in Chemical Engineering and Science 2015年第3期5卷 239-261页

作者： Silvana C. Plem Diana m. müller marcelo C. murguía Laboratorio de Química Aplicada (LAQUImAP) Facultad de Bioquímica y Ciencias Biológicas Universidad Nacional del Litoral (UNL) Santa Fe Argentina

A series of six pyrazoles was synthesized by michael-type addition reaction. The molecules 5-amino-1-aryl-1H-pyrazole-4-carbonitrile (3a-f) were synthesized from (ethoxymethylene)malo-nonitrile (1) and fluorinated and non-fluorinated aryl hydrazines (2a-f) using ethanol and fluorinated ethanol as solvents at reflux. An excellent regio-selectivity was found when pyrazole derivatives were formed as an exclusive product. No other regioisomer or uncyclised hydrazide was observed. Their structures were confirmed by spectroscopy data (1H, 13C, 19F, COSY (correlation spectroscopy), HSQC (heteronuclear single-quantum correlation spectroscopy) and HmBC (heteronuclear multiple-bond correlation spectroscopy);mS (mass-spectrometry). The yields ranged from good to excellent (47% - 93%) under mild reaction conditions. It would indicate a high selectivity in the one-step work procedure. These products (3a-f) and derivatives have a potential academic and industrial use as key intermediates, in special, for application in crop protection.

关键词： Aryl Pyrazole Aryl Hydrazine Amino Pyrazole 5-Amino-1-aryl-1H-pyrazole-4-carbonitriles

用0．5mV端电压的串列AmS装置开展10Be测量方法研究

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原子能科学技术 2013年第11期47卷 2131-2136页

作者：付东坡丁杏芳 A.m.maller m.Suter 周力平刘克新北京大学重离子物理研究所核物理与核技术国家重点实验室北京100871 苏黎世联邦理工学院离子束物理实验室苏黎世8093 北京大学地理系地表过程分析与模拟教育部重点实验室北京100871

本文通过对10Be离子在加速器质谱计(CAmS)高能端束流传输进行模拟计算,确定了10 Be离子束流传输的优化设计。设计安装了测量9Be束流的法拉第筒、能量吸收膜(SiN膜)装置、ΔE-Eres气体探测器等,结果表明,改进后的CAmS可进行10Be测量,10B... 详细信息

本文通过对10Be离子在加速器质谱计(CAmS)高能端束流传输进行模拟计算,确定了10 Be离子束流传输的优化设计。设计安装了测量9Be束流的法拉第筒、能量吸收膜(SiN膜)装置、ΔE-Eres气体探测器等,结果表明,改进后的CAmS可进行10Be测量,10Be测量总传输效率约为2%,测量本底约为4×10-14。

关键词：加速器质谱 10Be 传输效率本底

Nano-and micro-patterned Biomaterials

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复旦学报（自然科学版） 2007年第5期46卷 706-页

作者： m.C.Lensen m.Diez V.A.Schulte P.mela m.mller DWI e.V.and Institute of Technical and macromolecular Chemistry RWTH AachenGermany

1 Results Our objective is two fold: we (ⅰ) aim at the development of novel patterning methodologies in order to (ⅱ) achieve control over the positioning and alignment of living *** patterning of the biointerfaces is carried out both at the micro-and nanometer scale and involve (bio)chemical as well as topographic *** former are relatively easily obtained by patterning techniques adapted from (conventional) soft lithography,*** means of micro-contact printing (μ-CP).The topographic pat...

关键词： soft lithography hydrogels nanoimprinting UV-curing cell adhesion

New Amphiphilic Amino Acid Derivatives for Efficient DNA Transfection in Vitro

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Advances in Chemical Engineering and Science 2017年第2期7卷 191-205页

作者： Lucía C.Pena maría F.Argaraná maría m.De Zan Antonella Giorello Sebastián Antuna Claudio C.Prieto Carolina m.I.Veaute Diana m.müller LAQUImAP Dto.Química OrgánicaUniversidad Nacional del LitoralSanta FeArgentina Cát.microbiología General Universidad Nacional del LitoralSanta FeArgentina Laoratorio de Control de medicamentos Universidad Nacional del LitoralSanta FeArgentina Instituto de Investigaciones en Catálisis y Petroquímica Universidad Nacional del LitoralSanta FeArgentina Laboratorio de Cultivos Celulares Universidad Nacional del LitoralSanta FeArgentina Laboratorio de Inmunología Básica Universidad Nacional del LitoralSanta FeArgentina

Nucleic acids-based therapies have recently developed as next-generation agents for treating and preventing viral infection, cancer, and genetic disorders, but their use is still limited due to its relatively poor delivery into targeted cells. We designed and synthesized new amphiphilic amino acid derivatives (cysteine-based) of low molecular weight, formed by the same pentapeptide (AG2: WWCOO) N-acylated, with different hydrophobic chains containing from 12 to 18 carbons, named AG2-Cn (N), which dimerize by oxidation in the presence of pLenti-CmV-GFP Puro plasmid (P) in the respective gemini. We determined transfection efficiency, critical micelle concentration, particle size, ζ-potential and cytotoxicity for the derivatives obtained. We found that all the synthesized compounds were active for DNA delivery and had greater ability to transfect CHO-K1 cells. In particular, AG2-C18 is a promising carrier for gene delivery because it showed no cytotoxicity and its activity was greater than or equal to the commercial actives currently used.

关键词： Amphiphile N-Acylated Cysteine Gemini Ornithine Transfection

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彩色电视屏用光敏磷光体粉浆的研磨条件

发光学报 1975年第3期 7-9页

作者： m.mller 周国华 AEG电话公司西德

美国电化学学会第145次会议文章摘要中,有53篇和发光材料、显示器件有关。我们从中选译了一部份,陆续刊登以供参考。这次刊出的是和彩色电视有关的文章摘要,参考资料目录一律从略。

关键词：磷光体粉浆扫描电子显微照片润湿能力